Rh6DG028700

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000434
Physical Location & Seq
Reverse (-)
2584440 .. 2594281
9842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG028700.1

Sequence Viewer

Length: 1944 bp
ATGGTGAGCCATGGAGGTGGTGGTGGTGGTGGTGGTGCGACTACTACCTCCAAAGCCCACCGCCTGGTGTTCATTCTCCCAATCATTATCATCTTTGGATTGCTTTCATGGGTTTTCACTTCTCACGTGGTCTCTACTCCAAAGTCTTCAACAAAGATTGGATCCAGTTTTGTTGTTGGTGTTGAAGGGTACGGTACTACAATGGAAAAAAATCACTCTCAGATCATTAATGGGACAAGACATTATACTAATCTAGAGAGGCTGGAGGGGCGTCTACGACGAGCTCGTGTCACAATTAAGATGGGAAACCGATCACAAGATTCCGACTACATCCCCCATGGACCAATGTATTGGAATGCTAATGCATTTCACAGAAGCTATATTGAAATGGAGAAACAGTTCAAGGTGTTTGTGTATGGAGAAGGGGAACTTCCATTGTTTCATAATGGGCCTTGCAAAAGCATATATTCCATGGAGGGGAACTTTATACATGAACTGGAGAAATTAATGAACGTCTTGACAATATTTTTGTTCTTAATGTTGGCGTTTACAACTAAAATTCAATCCTCTGTTGTCTTTGACGTGACAAGTGCAAAATATGGGGGAAAGCCAAACTCAGACATTACAAAGGCTTTGCTGAATGCTTGGACTGATGCATGTGCATCACCGAGGGAGAGTAAAGTTCTTGTTCCAAAAGGCACCTACGGATTAAAAGGAGCAATTTTCAGAGGTCATTGCAAGGCTTCTATTGAGCTTCAGGTCCGAGGCACATTGCAGGCTCCAAAAGACACTAGCCAAGTCACATCACCGGATACTTGGGTTGGTTTTCGGTACCTTAATAGGCTCACCTTGTCAGGTGGTGGAACTTTTGATGGCCGCGGAGCACTCTCTTGGAAACAGAACGACTGTAACGAAAACAAAAACTGCAAATCTCGGGTCGTGAACATAAGGTTCGACTTCGTCAACGATACAATAATTAAGGACATAACTTCACTTGACAGCAAGAACTTTCACTTCAACGTTTTCGCCTGCCACAATGTTACTTTCCAACATGCAACCATCACAGCACCGGGAGAGAGTGTTAACACAGACGGTATCCACATTGCACGTTCAACAATGGTCACCGTCGCCAACACGAGCATCGGAACCGGAGACGATTGTATTTCCATCGGTGATGGGACTTCACAACTTAAATTTACCAACGTGACTTGCGGACCAGGCCATGGCATAAGTATTGGAAGCCTCGGAGGGCACCGCGGTGAACAACCTGTAACTGGCGTCATAATCAAGAACGTAACATTCAATAATACGCAAAATGGTGCAAGAATCAAGACGTGGCCAGCCTCCTATGCCGGCCTTGTGTCAGATATACACTATGAGGATATTACAATGGTTAATGTCAGCAACCCGATCATTATAGACCAAGAGTACTGCCCATGGAATTTGTGCAACAAGCAGATTCCTTCAAAAGTTAAGATCAGTAATGTGAGCTTCAAGAACATTAAAGGCACGTCCACAACTCAGCTTGCGGTAAATTTTATTTGTAGCAGGAGCGTGCCATGCGAGAATGTGGAGATTGCCGGCATTGATCTCACGTATACTGGACACAAAGGACTTATTAATTCACAATGCTCCAATGTCAAACTGGCGACGATTGTTAACGTGACAAAGGCTCTTGCTTGTGGTAACTATCCACCAATAATTACATCAAAGAACCACACCAAGAGTCATACTCCAACAATTAGTCGGTCAAAGAATTACCAGACCGAGACGAAAAACCAGACTGATTCTGTTGTAGGGAATGAGCCAGCCAGAGGTCATCAACGTAAGTATGCTCTGATTGCGACGTCCTTGGCCCCAAAAAGTGATGATTACTTTTCAACAGTGACGCCCTCCGCTCCAAAGAGTCGCAGTACTTATGGGTGGCTACAACCGATGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

647

Amino Acids

70.7

Weight (kDa)

9.29

Isoelectric Point (pI)

31.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 220 - 548 3.8e-98 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1850
Acc65I GGTACC 1 cut(s) 831
AccB1I GGYRCC 3 cut(s) 698, 831, 1251
AccB7I CCANNNNNTGG 2 cut(s) 64, 1223
AccBSI CCGCTC 1 cut(s) 1898
AccI GTMKAC 2 cut(s) 274, 1598
AccII CGCG 2 cut(s) 879, 1257
AciI CCGC 8 cut(s) 61, 877, 879, 1212, 1255, 1257, 1529, 1896
AclI AACGTT 1 cut(s) 1020
AclWI GGATC 2 cut(s) 156, 169
AcoI YGGCCR 2 cut(s) 874, 1337
AcsI RAATTY 4 cut(s) 558, 1193, 1441, 1534
AcuI CTGAAG 1 cut(s) 740
AcvI CACGTG 1 cut(s) 127
AcyI GRCGYC 4 cut(s) 271, 1278, 1847, 1889
AfaI GTAC 5 cut(s) 191, 196, 833, 1430, 1915
AfiI CCNNNNNNNGG 5 cut(s) 64, 477, 1223, 1274, 1814
AjiI CACGTC 3 cut(s) 583, 1335, 1512
AjnI CCWGG 2 cut(s) 63, 1216
AleI CACNNNNGTG 1 cut(s) 1257
AloI GAACNNNNNNTCC 2 cut(s) 383, 415
AluBI AGCT 5 cut(s) 284, 378, 754, 1491, 1525
AluI AGCT 5 cut(s) 284, 378, 754, 1491, 1525
Alw21I GWGCWC 2 cut(s) 286, 886
Alw26I GTCTC 3 cut(s) 136, 1146, 1763
AlwI GGATC 2 cut(s) 156, 169
Ama87I CYCGRG 1 cut(s) 933
AoxI GGCC 6 cut(s) 449, 874, 1219, 1337, 1354, 1854
ApoI RAATTY 4 cut(s) 558, 1193, 1441, 1534
ArsI GACNNNNNNTTYG 4 cut(s) 511, 543, 921, 953
AseI ATTAAT 3 cut(s) 228, 506, 1620
Asp700I GAANNNNTTC 1 cut(s) 398
Asp718I GGTACC 1 cut(s) 831
AspS9I GGNCC 5 cut(s) 341, 449, 760, 1214, 1855
AsuC2I CCSGG 1 cut(s) 1071
AsuHPI GGTGA 7 cut(s) 16, 657, 798, 838, 1114, 1184, 1271
AvaI CYCGRG 1 cut(s) 933
AvaII GGWCC 3 cut(s) 341, 760, 1214
BaeGI GKGCMC 1 cut(s) 1254
BaeI ACNNNNGTAYC 2 cut(s) 804, 837
BalI TGGCCA 1 cut(s) 1339
BamHI GGATCC 1 cut(s) 161
BanI GGYRCC 3 cut(s) 698, 831, 1251
BanII GRGCYC 1 cut(s) 286
BauI CACGAG 2 cut(s) 285, 1135
BbrPI CACGTG 1 cut(s) 127
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 2 cut(s) 286, 886
BccI CCATC 5 cut(s) 295, 866, 1067, 1169, 1175
BciT130I CCWGG 2 cut(s) 65, 1218
BciVI GTATCC 2 cut(s) 805, 1106
BcnI CCSGG 1 cut(s) 1071
BcoDI GTCTC 3 cut(s) 136, 1146, 1763
BfaI CTAG 2 cut(s) 254, 792
BfuI GTATCC 2 cut(s) 805, 1106
BisI GCNGC 1 cut(s) 877
BlsI GCNGC 1 cut(s) 878
BmcAI AGTACT 2 cut(s) 1430, 1915
Bme1390I CCNGG 3 cut(s) 65, 1071, 1218
Bme18I GGWCC 3 cut(s) 341, 760, 1214
BmeT110I CYCGRG 1 cut(s) 933
BmgBI CACGTC 3 cut(s) 583, 1335, 1512
BmgT120I GGNCC 5 cut(s) 341, 449, 760, 1214, 1855
BmiI GGNNCC 7 cut(s) 163, 700, 780, 833, 1147, 1253, 1857
BmrFI CCNGG 3 cut(s) 65, 1071, 1218
BmsI GCATC 4 cut(s) 643, 671, 1149, 1926
BpiI GAAGAC 1 cut(s) 138
BplI GAGNNNNNCTC 2 cut(s) 1717, 1749
BpmI CTGGAG 2 cut(s) 284, 518
BpuMI CCSGG 1 cut(s) 1071
BsaAI YACGTR 2 cut(s) 127, 1596
BsaHI GRCGYC 4 cut(s) 271, 1278, 1847, 1889
BsaI GGTCTC 1 cut(s) 136
BsaWI WCCGGW 2 cut(s) 808, 1148
BsaXI ACNNNNNCTCC 4 cut(s) 383, 413, 1065, 1095
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 477, 1223, 1274, 1814
Bse118I RCCGGY 2 cut(s) 1352, 1580
Bse1I ACTGG 5 cut(s) 165, 501, 1279, 1606, 1650
Bse3DI GCAATG 3 cut(s) 733, 770, 1101
BseBI CCWGG 2 cut(s) 65, 1218
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 5 cut(s) 64, 477, 1223, 1274, 1814
BseMI GCAATG 3 cut(s) 733, 770, 1101
BseMII CTCAG 3 cut(s) 233, 630, 1535
BseNI ACTGG 5 cut(s) 165, 501, 1279, 1606, 1650
BseSI GKGCMC 1 cut(s) 1254
Bsh1236I CGCG 2 cut(s) 879, 1257
BshFI GGCC 6 cut(s) 451, 876, 1221, 1339, 1356, 1856
BshNI GGYRCC 3 cut(s) 698, 831, 1251
BsiHKAI GWGCWC 2 cut(s) 286, 886
BsiHKCI CYCGRG 1 cut(s) 933
BsiSI CCGG 5 cut(s) 809, 1070, 1149, 1353, 1581
BslFI GGGAC 2 cut(s) 247, 1192
BslI CCNNNNNNNGG 5 cut(s) 64, 477, 1223, 1274, 1814
BsmAI GTCTC 3 cut(s) 136, 1146, 1763
BsmBI CGTCTC 2 cut(s) 1146, 1763
BsmFI GGGAC 2 cut(s) 247, 1192
BsmI GAATGC 2 cut(s) 361, 646
BsnI GGCC 6 cut(s) 451, 876, 1221, 1339, 1356, 1856
Bso31I GGTCTC 1 cut(s) 136
BsoBI CYCGRG 1 cut(s) 933
Bsp1286I GDGCHC 3 cut(s) 286, 886, 1254
Bsp143I GATC 6 cut(s) 161, 222, 311, 1410, 1476, 1588
Bsp19I CCATGG 5 cut(s) 10, 337, 471, 1222, 1436
BspACI CCGC 8 cut(s) 61, 877, 879, 1212, 1255, 1257, 1529, 1896
BspANI GGCC 6 cut(s) 451, 876, 1221, 1339, 1356, 1856
BspCNI CTCAG 3 cut(s) 232, 629, 1534
BspFNI CGCG 2 cut(s) 879, 1257
BspLI GGNNCC 7 cut(s) 163, 700, 780, 833, 1147, 1253, 1857
BspPI GGATC 2 cut(s) 156, 169
BspT107I GGYRCC 3 cut(s) 698, 831, 1251
BspTNI GGTCTC 1 cut(s) 136
BsrBI CCGCTC 1 cut(s) 1898
BsrDI GCAATG 3 cut(s) 733, 770, 1101
BsrFI RCCGGY 2 cut(s) 1352, 1580
BsrI ACTGG 5 cut(s) 165, 501, 1279, 1606, 1650
BssAI RCCGGY 2 cut(s) 1352, 1580
BssMI GATC 6 cut(s) 161, 222, 311, 1410, 1476, 1588
BssNAI GTATAC 1 cut(s) 1599
BssNI GRCGYC 4 cut(s) 271, 1278, 1847, 1889
BssSI CACGAG 2 cut(s) 285, 1135
BssT1I CCWWGG 6 cut(s) 10, 337, 471, 1222, 1436, 1851
Bst1107I GTATAC 1 cut(s) 1599
Bst2BI CACGAG 2 cut(s) 285, 1135
Bst2UI CCWGG 2 cut(s) 65, 1218
Bst4CI ACNGT 6 cut(s) 194, 399, 908, 1094, 1126, 1885
BstACI GRCGYC 4 cut(s) 271, 1278, 1847, 1889
BstBAI YACGTR 2 cut(s) 127, 1596
BstC8I GCNNGC 8 cut(s) 777, 1030, 1341, 1354, 1527, 1556, 1582, 1809
BstDEI CTNAG 3 cut(s) 219, 616, 1521
BstDSI CCRYGG 7 cut(s) 10, 337, 471, 877, 1222, 1255, 1436
BstEII GGTNACC 1 cut(s) 1120
BstF5I GGATG 1 cut(s) 330
BstFNI CGCG 2 cut(s) 879, 1257
BstKTI GATC 6 cut(s) 164, 225, 314, 1413, 1479, 1591
BstMAI GTCTC 3 cut(s) 136, 1146, 1763
BstMBI GATC 6 cut(s) 161, 222, 311, 1410, 1476, 1588
BstMWI GCNNNNNNNGC 5 cut(s) 268, 1218, 1349, 1560, 1841
BstNI CCWGG 2 cut(s) 65, 1218
BstNSI RCATGY 2 cut(s) 660, 1055
BstPI GGTNACC 1 cut(s) 1120
BstSCI CCNGG 3 cut(s) 63, 1069, 1216
BstSLI GKGCMC 1 cut(s) 1254
BstUI CGCG 2 cut(s) 879, 1257
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 161
BstXI CCANNNNNNTGG 2 cut(s) 17, 351
BstYI RGATCY 1 cut(s) 161
BstZ17I GTATAC 1 cut(s) 1599
BsuI GTATCC 2 cut(s) 805, 1106
BsuRI GGCC 6 cut(s) 451, 876, 1221, 1339, 1356, 1856
BtgI CCRYGG 7 cut(s) 10, 337, 471, 877, 1222, 1255, 1436
BtrI CACGTC 3 cut(s) 583, 1335, 1512
BtsCI GGATG 1 cut(s) 330
BtsIMutI CAGTG 1 cut(s) 1890
Cac8I GCNNGC 8 cut(s) 777, 1030, 1341, 1354, 1527, 1556, 1582, 1809
Cfr10I RCCGGY 2 cut(s) 1352, 1580
Cfr13I GGNCC 5 cut(s) 341, 449, 760, 1214, 1855
Cfr42I CCGCGG 2 cut(s) 880, 1258
CseI GACGC 3 cut(s) 260, 1267, 1897
Csp6I GTAC 5 cut(s) 190, 195, 832, 1429, 1914
CviQI GTAC 5 cut(s) 190, 195, 832, 1429, 1914
DdeI CTNAG 3 cut(s) 219, 616, 1521
DpnI GATC 6 cut(s) 163, 224, 313, 1412, 1478, 1590
DpnII GATC 6 cut(s) 161, 222, 311, 1410, 1476, 1588
EaeI YGGCCR 2 cut(s) 874, 1337
Ecl136II GAGCTC 1 cut(s) 284
Eco130I CCWWGG 6 cut(s) 10, 337, 471, 1222, 1436, 1851
Eco24I GRGCYC 1 cut(s) 286
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 3 cut(s) 341, 760, 1214
Eco53kI GAGCTC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 740
Eco72I CACGTG 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 933
Eco91I GGTNACC 1 cut(s) 1120
EcoICRI GAGCTC 1 cut(s) 284
EcoO65I GGTNACC 1 cut(s) 1120
EcoRII CCWGG 2 cut(s) 63, 1216
EcoT14I CCWWGG 6 cut(s) 10, 337, 471, 1222, 1436, 1851
EcoT22I ATGCAT 2 cut(s) 367, 658
EcoT38I GRGCYC 1 cut(s) 286
ErhI CCWWGG 6 cut(s) 10, 337, 471, 1222, 1436, 1851
Esp3I CGTCTC 2 cut(s) 1146, 1763
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FaqI GGGAC 2 cut(s) 247, 1192
FblI GTMKAC 2 cut(s) 274, 1598
Fnu4HI GCNGC 1 cut(s) 877
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 877
FspBI CTAG 2 cut(s) 254, 792
GluI GCNGC 1 cut(s) 877
GsuI CTGGAG 2 cut(s) 284, 518
HaeIII GGCC 6 cut(s) 451, 876, 1221, 1339, 1356, 1856
HapII CCGG 5 cut(s) 809, 1070, 1149, 1353, 1581
HgaI GACGC 3 cut(s) 260, 1267, 1897
Hin1I GRCGYC 4 cut(s) 271, 1278, 1847, 1889
HincII GTYRAC 3 cut(s) 964, 1084, 1660
HindII GTYRAC 3 cut(s) 964, 1084, 1660
HinfI GANTC 6 cut(s) 320, 1326, 1459, 1726, 1787, 1906
HpaI GTTAAC 2 cut(s) 1084, 1660
HpaII CCGG 5 cut(s) 809, 1070, 1149, 1353, 1581
HphI GGTGA 7 cut(s) 16, 657, 798, 838, 1114, 1184, 1271
Hpy166II GTNNAC 9 cut(s) 275, 549, 943, 964, 1084, 1262, 1515, 1599, 1660
Hpy188I TCNGA 9 cut(s) 222, 325, 619, 728, 764, 1145, 1247, 1366, 1839
Hpy188III TCNNGA 6 cut(s) 254, 517, 940, 1288, 1330, 1495
Hpy8I GTNNAC 9 cut(s) 275, 549, 943, 964, 1084, 1262, 1515, 1599, 1660
Hpy99I CGWCG 4 cut(s) 282, 1130, 1654, 1849
HpyAV CCTTC 3 cut(s) 179, 416, 1473
HpyCH4III ACNGT 6 cut(s) 194, 399, 908, 1094, 1126, 1885
HpyF10VI GCNNNNNNNGC 5 cut(s) 268, 1218, 1349, 1560, 1841
HpyF3I CTNAG 3 cut(s) 219, 616, 1521
Hsp92I GRCGYC 4 cut(s) 271, 1278, 1847, 1889
KpnI GGTACC 1 cut(s) 835
KroI GCCGGC 2 cut(s) 1352, 1580
KroNI GCCGGC 2 cut(s) 1354, 1582
KspAI GTTAAC 2 cut(s) 1084, 1660
KspI CCGCGG 2 cut(s) 880, 1258
Kzo9I GATC 6 cut(s) 161, 222, 311, 1410, 1476, 1588
LmnI GCTCC 6 cut(s) 716, 784, 881, 1551, 1637, 1903
LweI GCATC 4 cut(s) 643, 671, 1149, 1926
MaeI CTAG 2 cut(s) 254, 792
MalI GATC 6 cut(s) 163, 224, 313, 1412, 1478, 1590
MbiI CCGCTC 1 cut(s) 1898
MboI GATC 6 cut(s) 161, 222, 311, 1410, 1476, 1588
MboII GAAGA 1 cut(s) 138
MflI RGATCY 1 cut(s) 161
MhlI GDGCHC 3 cut(s) 286, 886, 1254
MlsI TGGCCA 1 cut(s) 1339
MluNI TGGCCA 1 cut(s) 1339
MlyI GAGTC 2 cut(s) 1735, 1915
MmeI TCCRAC 3 cut(s) 348, 1072, 1760
Mox20I TGGCCA 1 cut(s) 1339
Mph1103I ATGCAT 2 cut(s) 367, 658
MroNI GCCGGC 2 cut(s) 1352, 1580
MroXI GAANNNNTTC 1 cut(s) 398
MscI TGGCCA 1 cut(s) 1339
MslI CAYNNNNRTG 2 cut(s) 15, 1257
Msp20I TGGCCA 1 cut(s) 1339
MspA1I CMGCKG 2 cut(s) 879, 1257
MspI CCGG 5 cut(s) 809, 1070, 1149, 1353, 1581
MspR9I CCNGG 3 cut(s) 65, 1071, 1218
Mva1269I GAATGC 2 cut(s) 361, 646
MvaI CCWGG 2 cut(s) 65, 1218
MvnI CGCG 2 cut(s) 879, 1257
MwoI GCNNNNNNNGC 5 cut(s) 268, 1218, 1349, 1560, 1841
NaeI GCCGGC 2 cut(s) 1354, 1582
NciI CCSGG 1 cut(s) 1071
NcoI CCATGG 5 cut(s) 10, 337, 471, 1222, 1436
NdeII GATC 6 cut(s) 161, 222, 311, 1410, 1476, 1588
NgoMIV GCCGGC 2 cut(s) 1352, 1580
NlaIV GGNNCC 7 cut(s) 163, 700, 780, 833, 1147, 1253, 1857
NmuCI GTSAC 7 cut(s) 289, 583, 799, 1120, 1204, 1663, 1885
NsiI ATGCAT 2 cut(s) 367, 658
NspI RCATGY 2 cut(s) 660, 1055
OliI CACNNNNGTG 1 cut(s) 1257
PcsI WCGNNNNNNNCGW 2 cut(s) 283, 909
PctI GAATGC 2 cut(s) 361, 646
PdiI GCCGGC 2 cut(s) 1354, 1582
PdmI GAANNNNTTC 1 cut(s) 398
PfeI GAWTC 4 cut(s) 320, 1326, 1459, 1787
PflFI GACNNNGTC 1 cut(s) 959
PflMI CCANNNNNTGG 2 cut(s) 64, 1223
PkrI GCNGC 1 cut(s) 878
PleI GAGTC 2 cut(s) 1734, 1914
PmaCI CACGTG 1 cut(s) 127
PmlI CACGTG 1 cut(s) 127
PpsI GAGTC 2 cut(s) 1734, 1914
Ppu21I YACGTR 2 cut(s) 127, 1596
PshBI ATTAAT 3 cut(s) 228, 506, 1620
Psp124BI GAGCTC 1 cut(s) 286
Psp1406I AACGTT 1 cut(s) 1020
Psp6I CCWGG 2 cut(s) 63, 1216
PspCI CACGTG 1 cut(s) 127
PspEI GGTNACC 1 cut(s) 1120
PspGI CCWGG 2 cut(s) 63, 1216
PspN4I GGNNCC 7 cut(s) 163, 700, 780, 833, 1147, 1253, 1857
PspPI GGNCC 5 cut(s) 341, 449, 760, 1214, 1855
PsuI RGATCY 1 cut(s) 161
PsyI GACNNNGTC 1 cut(s) 959
RsaI GTAC 5 cut(s) 191, 196, 833, 1430, 1915
RsaNI GTAC 5 cut(s) 190, 195, 832, 1429, 1914
RseI CAYNNNNRTG 2 cut(s) 15, 1257
SacI GAGCTC 1 cut(s) 286
SacII CCGCGG 2 cut(s) 880, 1258
SatI GCNGC 1 cut(s) 877
Sau3AI GATC 6 cut(s) 161, 222, 311, 1410, 1476, 1588
Sau96I GGNCC 5 cut(s) 341, 449, 760, 1214, 1855
ScaI AGTACT 2 cut(s) 1430, 1915
SchI GAGTC 2 cut(s) 1735, 1915
ScrFI CCNGG 3 cut(s) 65, 1071, 1218
SduI GDGCHC 3 cut(s) 286, 886, 1254
SfaNI GCATC 4 cut(s) 643, 671, 1149, 1926
Sfr303I CCGCGG 2 cut(s) 880, 1258
SgrBI CCGCGG 2 cut(s) 880, 1258
SinI GGWCC 3 cut(s) 341, 760, 1214
SmiMI CAYNNNNRTG 2 cut(s) 15, 1257
SsiI CCGC 8 cut(s) 61, 877, 879, 1212, 1255, 1257, 1529, 1896
SspI AATATT 1 cut(s) 525
SspMI CTAG 2 cut(s) 254, 792
SstI GAGCTC 1 cut(s) 286
StyD4I CCNGG 3 cut(s) 63, 1069, 1216
StyI CCWWGG 6 cut(s) 10, 337, 471, 1222, 1436, 1851
TaaI ACNGT 6 cut(s) 194, 399, 908, 1094, 1126, 1885
TaqI TCGA 1 cut(s) 954
TaqII GACCGA 2 cut(s) 1737, 1781
TatI WGTACW 2 cut(s) 1428, 1913
TauI GCSGC 1 cut(s) 879
TfiI GAWTC 4 cut(s) 320, 1326, 1459, 1787
TscAI CASTG 1 cut(s) 1890
TseFI GTSAC 7 cut(s) 289, 583, 799, 1120, 1204, 1663, 1885
Tsp45I GTSAC 7 cut(s) 289, 583, 799, 1120, 1204, 1663, 1885
TspDTI ATGAA 5 cut(s) 61, 96, 431, 507, 524
TspGWI ACGGA 1 cut(s) 720
TspRI CASTG 1 cut(s) 1890
Tth111I GACNNNGTC 1 cut(s) 959
Van91I CCANNNNNTGG 2 cut(s) 64, 1223
VpaK11BI GGWCC 3 cut(s) 341, 760, 1214
VspI ATTAAT 3 cut(s) 228, 506, 1620
XapI RAATTY 4 cut(s) 558, 1193, 1441, 1534
XbaI TCTAGA 1 cut(s) 253
XceI RCATGY 2 cut(s) 660, 1055
XcmI CCANNNNNNNNNTGG 2 cut(s) 17, 1642
XmiI GTMKAC 2 cut(s) 274, 1598
XmnI GAANNNNTTC 1 cut(s) 398
XspI CTAG 2 cut(s) 254, 792
ZraI GACGTC 1 cut(s) 1848
ZrmI AGTACT 2 cut(s) 1430, 1915
Zsp2I ATGCAT 2 cut(s) 367, 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.