Rh2DG545200

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000434
Physical Location & Seq
Forward (+)
77411487 .. 77415478
3992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG545200.1

Sequence Viewer

Length: 1248 bp
ATGGCTCTCAAACTTGTCATGGTTATGTTTCCATTACTTATGTTCTTATCCTTGACATCCTCGACATCTGGAAGGAGAGAGCTTCTAGCCGATGGTGGTGACTTTGACGTGACCAACGCGAAATATGGTGCAAAGCCCGATTCTGATATTACTGAGGCTTTAGCAAATGCTTGGAAAGACGCCTGTGCATCAACATCGGCAAGTAAAGTTATTGTTCCGAAAGGGACATTCAAAGTAAAAGAAGCAAGTTTCAAAGGTCCTTGCAAGGCTCCTATTGAGGTTCAGGTCCAAGGCACATTACAGGCGCCAGAAGACTCAAGCCAATTATCGAAACAAGATACTTGGATCGAGTTTTTATACCTCAACGACTTCACACTATCGGGTGGTGGAACCTTTGATGGCCAAGGACAAAAAACTTGGAAAGCCATTGATCCAAAAAACGCAAAGGCTGGCACCATTAATATCAGGTTCCACGCCGTCAAGAAGTCATTAGTTAAGGATATAACTTCACTTAATAGCAAGAACTTCCACATCAACGTAATAAGTTGCGAGCAACTTACGTTCGATCATGTCACCGTCACTGCACCTGGAGATAGCCCTAACACAGATGGAATACATATCGCGCGCTCAACCGGGGTCAACGTTACTGATACAAATATCGGAACCGGAGATGATTGCATTTCAATTGGTGATGGTACCAAGCAACTTACAATAACTAAGGTTACTTGTGGACCAGGCCATGGAATAAGCGTTGGAAGTCTTGGAAGGTACGACAAAGAAGACCCTGTGGAAGGAATCAATGTTAAGAACTGTACCCTCAGTAAAACGACTAATGGTGTTAGAATCAAGACATGGGCTAAAAATCCTCAAGCTAGCACTTGCTCAGAAATTCACTTTGAGGATATCATCATGAATGATGTTCAAAACCCAATCGTCGTAGATCAAGAATACTGCCCATGGGATCAATGTGATAAACAGGCTTCATCGAAAATCAAGATCAGCGATGTGAGTTTCAAGAACATTAGAGGGACGACTTCCACTCCGTTGGGCGTGAAGATTGTATGCGCCAAGGGCTTACCATGTGAGAAGGTGGAGATGACTGGCATTGATCTCAAATTCACCGGCACAGGTGCACTCACGTCTCAATGTACGAATGTCAAACCCACAATTGCTAATGTGGCACAGCCTCTTGCTTGTGCTACCGATGCTCCTGTACAGGCTGGACCAGCTGAGAAGTCTAATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

415

Amino Acids

44.28

Weight (kDa)

6.33

Isoelectric Point (pI)

27.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 62 - 388 7.2e-102 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 695
AccB1I GGYRCC 3 cut(s) 304, 452, 695
AccB7I CCANNNNNTGG 1 cut(s) 740
AccII CGCG 3 cut(s) 119, 623, 625
AclI AACGTT 1 cut(s) 642
AclWI GGATC 3 cut(s) 353, 425, 969
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 2 cut(s) 888, 1115
AcyI GRCGYC 2 cut(s) 180, 305
AfaI GTAC 5 cut(s) 697, 770, 814, 1150, 1215
AfiI CCNNNNNNNGG 2 cut(s) 740, 791
AgsI TTSAA 5 cut(s) 232, 253, 684, 923, 1015
AjiI CACGTC 2 cut(s) 109, 1140
AjnI CCWGG 2 cut(s) 586, 733
AluBI AGCT 3 cut(s) 82, 872, 1229
AluI AGCT 3 cut(s) 82, 872, 1229
Alw21I GWGCWC 1 cut(s) 1135
Alw26I GTCTC 1 cut(s) 1146
Alw44I GTGCAC 1 cut(s) 1131
AlwI GGATC 3 cut(s) 353, 425, 969
AoxI GGCC 2 cut(s) 400, 736
ApaLI GTGCAC 1 cut(s) 1131
ApoI RAATTY 2 cut(s) 888, 1115
AseI ATTAAT 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 695
AspLEI GCGC 4 cut(s) 307, 625, 627, 1067
AspS9I GGNCC 4 cut(s) 257, 286, 731, 1223
AsuC2I CCSGG 1 cut(s) 634
AsuHPI GGTGA 4 cut(s) 110, 565, 701, 1111
AsuNHI GCTAGC 1 cut(s) 872
AvaII GGWCC 4 cut(s) 257, 286, 731, 1223
BaeGI GKGCMC 1 cut(s) 1135
BalI TGGCCA 1 cut(s) 402
BanI GGYRCC 3 cut(s) 304, 452, 695
BbsI GAAGAC 2 cut(s) 318, 786
Bbv12I GWGCWC 1 cut(s) 1135
BccI CCATC 4 cut(s) 86, 392, 602, 686
BceAI ACGGC 1 cut(s) 461
BcgI CGANNNNNNTGC 2 cut(s) 177, 211
BciT130I CCWGG 2 cut(s) 588, 735
BcnI CCSGG 1 cut(s) 634
BcoDI GTCTC 1 cut(s) 1146
BfaI CTAG 2 cut(s) 86, 873
BfoI RGCGCY 1 cut(s) 308
Bme1390I CCNGG 3 cut(s) 588, 634, 735
Bme18I GGWCC 4 cut(s) 257, 286, 731, 1223
BmgBI CACGTC 2 cut(s) 109, 1140
BmgT120I GGNCC 4 cut(s) 257, 286, 731, 1223
BmiI GGNNCC 7 cut(s) 270, 306, 391, 454, 470, 664, 697
BmrFI CCNGG 3 cut(s) 588, 634, 735
BmsI GCATC 2 cut(s) 197, 1195
BmtI GCTAGC 1 cut(s) 876
BpiI GAAGAC 2 cut(s) 318, 786
BpmI CTGGAG 1 cut(s) 609
BpuEI CTTGAG 2 cut(s) 301, 852
BpuMI CCSGG 1 cut(s) 634
BsaHI GRCGYC 2 cut(s) 180, 305
BsaJI CCNNGG 6 cut(s) 289, 403, 633, 739, 956, 1068
BsaWI WCCGGW 1 cut(s) 665
BsaXI ACNNNNNCTCC 4 cut(s) 1024, 1054, 1192, 1222
Bsc4I CCNNNNNNNGG 2 cut(s) 740, 791
Bse118I RCCGGY 1 cut(s) 1121
Bse1I ACTGG 1 cut(s) 1105
BseBI CCWGG 2 cut(s) 588, 735
BseDI CCNNGG 6 cut(s) 289, 403, 633, 739, 956, 1068
BseGI GGATG 1 cut(s) 56
BseLI CCNNNNNNNGG 2 cut(s) 740, 791
BseMII CTCAG 4 cut(s) 144, 832, 897, 1221
BseNI ACTGG 1 cut(s) 1105
BsePI GCGCGC 1 cut(s) 623
BseSI GKGCMC 1 cut(s) 1135
BsgI GTGCAG 1 cut(s) 567
Bsh1236I CGCG 3 cut(s) 119, 623, 625
BshFI GGCC 2 cut(s) 402, 738
BshNI GGYRCC 3 cut(s) 304, 452, 695
BsiHKAI GWGCWC 1 cut(s) 1135
BsiSI CCGG 3 cut(s) 633, 666, 1122
BslFI GGGAC 2 cut(s) 238, 1042
BslI CCNNNNNNNGG 2 cut(s) 740, 791
BsmAI GTCTC 1 cut(s) 1146
BsmBI CGTCTC 1 cut(s) 1146
BsmFI GGGAC 2 cut(s) 238, 1042
BsnI GGCC 2 cut(s) 402, 738
Bsp1286I GDGCHC 1 cut(s) 1135
Bsp1407I TGTACA 1 cut(s) 1213
Bsp143I GATC 7 cut(s) 345, 430, 565, 940, 961, 996, 1108
Bsp19I CCATGG 2 cut(s) 739, 956
BspANI GGCC 2 cut(s) 402, 738
BspCNI CTCAG 4 cut(s) 145, 831, 896, 1222
BspFNI CGCG 3 cut(s) 119, 623, 625
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 7 cut(s) 270, 306, 391, 454, 470, 664, 697
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 3 cut(s) 353, 425, 969
BspT107I GGYRCC 3 cut(s) 304, 452, 695
BsrFI RCCGGY 1 cut(s) 1121
BsrGI TGTACA 1 cut(s) 1213
BsrI ACTGG 1 cut(s) 1105
BssAI RCCGGY 1 cut(s) 1121
BssECI CCNNGG 6 cut(s) 289, 403, 633, 739, 956, 1068
BssHII GCGCGC 1 cut(s) 623
BssMI GATC 7 cut(s) 345, 430, 565, 940, 961, 996, 1108
BssNI GRCGYC 2 cut(s) 180, 305
BssT1I CCWWGG 5 cut(s) 289, 403, 739, 956, 1068
Bst2UI CCWGG 2 cut(s) 588, 735
Bst4CI ACNGT 2 cut(s) 577, 812
BstACI GRCGYC 2 cut(s) 180, 305
BstAUI TGTACA 1 cut(s) 1213
BstC8I GCNNGC 4 cut(s) 451, 551, 625, 874
BstDEI CTNAG 5 cut(s) 153, 717, 818, 883, 1230
BstDSI CCRYGG 2 cut(s) 739, 956
BstENI CCTNNNNNAGG 1 cut(s) 789
BstF5I GGATG 1 cut(s) 56
BstFNI CGCG 3 cut(s) 119, 623, 625
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 4 cut(s) 307, 625, 627, 1067
BstKTI GATC 7 cut(s) 348, 433, 568, 943, 964, 999, 1111
BstMAI GTCTC 1 cut(s) 1146
BstMBI GATC 7 cut(s) 345, 430, 565, 940, 961, 996, 1108
BstMWI GCNNNNNNNGC 4 cut(s) 1071, 1178, 1205, 1226
BstNI CCWGG 2 cut(s) 588, 735
BstSCI CCNGG 3 cut(s) 586, 632, 733
BstSLI GKGCMC 1 cut(s) 1135
BstUI CGCG 3 cut(s) 119, 623, 625
BstV2I GAAGAC 2 cut(s) 318, 786
BstXI CCANNNNNNTGG 1 cut(s) 1045
BsuRI GGCC 2 cut(s) 402, 738
BtgI CCRYGG 2 cut(s) 739, 956
BtgZI GCGATG 1 cut(s) 1017
BtrI CACGTC 2 cut(s) 109, 1140
BtsCI GGATG 1 cut(s) 56
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 4 cut(s) 451, 551, 625, 874
CciI TCATGA 1 cut(s) 909
CfoI GCGC 4 cut(s) 307, 625, 627, 1067
Cfr10I RCCGGY 1 cut(s) 1121
Cfr13I GGNCC 4 cut(s) 257, 286, 731, 1223
CseI GACGC 1 cut(s) 188
Csp6I GTAC 5 cut(s) 696, 769, 813, 1149, 1214
CviAII CATG 7 cut(s) 19, 569, 740, 852, 910, 957, 1080
CviQI GTAC 5 cut(s) 696, 769, 813, 1149, 1214
DdeI CTNAG 5 cut(s) 153, 717, 818, 883, 1230
DinI GGCGCC 1 cut(s) 306
DpnI GATC 7 cut(s) 347, 432, 567, 942, 963, 998, 1110
DpnII GATC 7 cut(s) 345, 430, 565, 940, 961, 996, 1108
EaeI YGGCCR 1 cut(s) 400
Eco130I CCWWGG 5 cut(s) 289, 403, 739, 956, 1068
Eco32I GATATC 1 cut(s) 904
Eco47I GGWCC 4 cut(s) 257, 286, 731, 1223
EcoNI CCTNNNNNAGG 1 cut(s) 789
EcoO109I RGGNCCY 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 586, 733
EcoRV GATATC 1 cut(s) 904
EcoT14I CCWWGG 5 cut(s) 289, 403, 739, 956, 1068
EgeI GGCGCC 1 cut(s) 306
EheI GGCGCC 1 cut(s) 306
ErhI CCWWGG 5 cut(s) 289, 403, 739, 956, 1068
Esp3I CGTCTC 1 cut(s) 1146
FaeI CATG 7 cut(s) 22, 572, 743, 855, 913, 960, 1083
FaqI GGGAC 2 cut(s) 238, 1042
FatI CATG 7 cut(s) 18, 568, 739, 851, 909, 956, 1079
FokI GGATG 1 cut(s) 43
FspBI CTAG 2 cut(s) 86, 873
GlaI GCGC 4 cut(s) 306, 624, 626, 1066
GsuI CTGGAG 1 cut(s) 609
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 2 cut(s) 402, 738
HapII CCGG 3 cut(s) 633, 666, 1122
HgaI GACGC 1 cut(s) 188
HhaI GCGC 4 cut(s) 307, 625, 627, 1067
Hin1I GRCGYC 2 cut(s) 180, 305
Hin1II CATG 7 cut(s) 22, 572, 743, 855, 913, 960, 1083
Hin6I GCGC 4 cut(s) 305, 623, 625, 1065
HinP1I GCGC 4 cut(s) 305, 623, 625, 1065
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HinfI GANTC 4 cut(s) 140, 314, 795, 843
HpaII CCGG 3 cut(s) 633, 666, 1122
HphI GGTGA 4 cut(s) 110, 565, 701, 1111
Hpy166II GTNNAC 3 cut(s) 640, 731, 1133
Hpy188I TCNGA 4 cut(s) 145, 219, 662, 886
Hpy188III TCNNGA 7 cut(s) 69, 481, 847, 910, 944, 994, 1015
Hpy8I GTNNAC 3 cut(s) 640, 731, 1133
Hpy99I CGWCG 1 cut(s) 938
HpyAV CCTTC 4 cut(s) 66, 759, 785, 1081
HpyCH4III ACNGT 2 cut(s) 577, 812
HpyCH4IV ACGT 5 cut(s) 108, 537, 560, 642, 1139
HpyCH4V TGCA 6 cut(s) 131, 188, 264, 584, 678, 1133
HpyF10VI GCNNNNNNNGC 4 cut(s) 1071, 1178, 1205, 1226
HpyF3I CTNAG 5 cut(s) 153, 717, 818, 883, 1230
HpySE526I ACGT 5 cut(s) 108, 537, 560, 642, 1139
Hsp92I GRCGYC 2 cut(s) 180, 305
Hsp92II CATG 7 cut(s) 22, 572, 743, 855, 913, 960, 1083
HspAI GCGC 4 cut(s) 305, 623, 625, 1065
KasI GGCGCC 1 cut(s) 304
KpnI GGTACC 1 cut(s) 699
Kzo9I GATC 7 cut(s) 345, 430, 565, 940, 961, 996, 1108
LmnI GCTCC 2 cut(s) 274, 1213
LweI GCATC 2 cut(s) 197, 1195
MaeI CTAG 2 cut(s) 86, 873
MaeII ACGT 5 cut(s) 108, 537, 560, 642, 1139
MaeIII GTNAC 6 cut(s) 98, 109, 571, 577, 643, 721
MalI GATC 7 cut(s) 347, 432, 567, 942, 963, 998, 1110
MboI GATC 7 cut(s) 345, 430, 565, 940, 961, 996, 1108
MboII GAAGA 3 cut(s) 323, 791, 1066
MfeI CAATTG 2 cut(s) 684, 1167
MhlI GDGCHC 1 cut(s) 1135
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 5 cut(s) 323, 684, 888, 1115, 1167
MluNI TGGCCA 1 cut(s) 402
Mly113I GGCGCC 1 cut(s) 305
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 733
MnlI CCTC 9 cut(s) 70, 148, 271, 371, 827, 876, 892, 1019, 1197
Mox20I TGGCCA 1 cut(s) 402
MscI TGGCCA 1 cut(s) 402
MseI TTAA 4 cut(s) 459, 495, 513, 804
MslI CAYNNNNRTG 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 1229
MspI CCGG 3 cut(s) 633, 666, 1122
MspR9I CCNGG 3 cut(s) 588, 634, 735
MunI CAATTG 2 cut(s) 684, 1167
MvaI CCWGG 2 cut(s) 588, 735
MvnI CGCG 3 cut(s) 119, 623, 625
MwoI GCNNNNNNNGC 4 cut(s) 1071, 1178, 1205, 1226
NarI GGCGCC 1 cut(s) 305
NciI CCSGG 1 cut(s) 634
NcoI CCATGG 2 cut(s) 739, 956
NdeII GATC 7 cut(s) 345, 430, 565, 940, 961, 996, 1108
NheI GCTAGC 1 cut(s) 872
NlaIII CATG 7 cut(s) 22, 572, 743, 855, 913, 960, 1083
NlaIV GGNNCC 7 cut(s) 270, 306, 391, 454, 470, 664, 697
NmuCI GTSAC 4 cut(s) 98, 109, 571, 577
PagI TCATGA 1 cut(s) 909
PauI GCGCGC 1 cut(s) 623
PfeI GAWTC 3 cut(s) 140, 795, 843
PflMI CCANNNNNTGG 1 cut(s) 740
PleI GAGTC 1 cut(s) 308
PluTI GGCGCC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
PpuMI RGGWCCY 1 cut(s) 257
PshBI ATTAAT 1 cut(s) 459
Psp1406I AACGTT 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 586, 733
PspGI CCWGG 2 cut(s) 586, 733
PspN4I GGNNCC 7 cut(s) 270, 306, 391, 454, 470, 664, 697
PspPI GGNCC 4 cut(s) 257, 286, 731, 1223
PspPPI RGGWCCY 1 cut(s) 257
PteI GCGCGC 1 cut(s) 623
PvuII CAGCTG 1 cut(s) 1229
RsaI GTAC 5 cut(s) 697, 770, 814, 1150, 1215
RsaNI GTAC 5 cut(s) 696, 769, 813, 1149, 1214
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 4 cut(s) 459, 495, 513, 804
Sau3AI GATC 7 cut(s) 345, 430, 565, 940, 961, 996, 1108
Sau96I GGNCC 4 cut(s) 257, 286, 731, 1223
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 3 cut(s) 588, 634, 735
SduI GDGCHC 1 cut(s) 1135
SfaNI GCATC 2 cut(s) 197, 1195
SfoI GGCGCC 1 cut(s) 306
SinI GGWCC 4 cut(s) 257, 286, 731, 1223
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 316, 867
SmoI CTYRAG 2 cut(s) 316, 867
Sse9I AATT 5 cut(s) 323, 684, 888, 1115, 1167
SspDI GGCGCC 1 cut(s) 304
SspMI CTAG 2 cut(s) 86, 873
StyD4I CCNGG 3 cut(s) 586, 632, 733
StyI CCWWGG 5 cut(s) 289, 403, 739, 956, 1068
TaaI ACNGT 2 cut(s) 577, 812
TaiI ACGT 5 cut(s) 111, 540, 563, 645, 1142
TaqI TCGA 5 cut(s) 62, 329, 348, 564, 986
TasI AATT 5 cut(s) 323, 684, 888, 1115, 1167
TatI WGTACW 1 cut(s) 1213
TfiI GAWTC 3 cut(s) 140, 795, 843
Tru1I TTAA 4 cut(s) 459, 495, 513, 804
Tru9I TTAA 4 cut(s) 459, 495, 513, 804
TscAI CASTG 1 cut(s) 586
TseFI GTSAC 4 cut(s) 98, 109, 571, 577
Tsp45I GTSAC 4 cut(s) 98, 109, 571, 577
TspDTI ATGAA 2 cut(s) 926, 972
TspGWI ACGGA 1 cut(s) 1032
TspRI CASTG 1 cut(s) 586
Van91I CCANNNNNTGG 1 cut(s) 740
VneI GTGCAC 1 cut(s) 1131
VpaK11BI GGWCC 4 cut(s) 257, 286, 731, 1223
VspI ATTAAT 1 cut(s) 459
XagI CCTNNNNNAGG 1 cut(s) 789
XapI RAATTY 2 cut(s) 888, 1115
XspI CTAG 2 cut(s) 86, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.