RLG00000015266

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000434
Physical Location & Seq
Forward (+)
63798888 .. 63799957
1070 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015266

Sequence Viewer

Length: 936 bp
ATGGCCCCCACTGGTCCCAACGCTTTGCTGAAAGCTTGGACTGATGCATGTGCATCACCGAGGGAGAGTAAAGTTCTTGTTCCAAGAGGCACCTACGGATTAAAAGGAGCAATTTTGAGAGGTCATTGCAAGGCTCCTATTGAACTTCAGGTTCGAGGCACTTTGCAGGCTCTAAAAGACACTAGCCGAGTCACGTCACCGGATACTTGGGTTGGTTTTCAGTACCTTAATAGGCTCACCTTGTCAGGTGGTGGAACTTTTGATGGCCGCGGAGCACTCTCTTGGAAACAGAACGACTGTAACGAAAACAAAAACTGCAAATCTCGGGTCGTGAACATAAGGTTCGACTTCGTCAACGATACAATAATTAAGGACATAACTTCACTTGACAGCAAGAACTTTCACTTCAACGTTTTCGCCTGCTACAATGATACTTTCCAACATGCAACCATCACAGCACCGGGAGAGAGTATTAACACAGAAGGTATCCACATTGCACGTTCAACGATGGTCACTGTCGCCAACACCAGCATCGGAACCGGAGACAATTGGATTTCCATCGGTGATGGGACTTCACAACTTAAATTTACCAACGTGACTTGCGGACCAGGCCATGGCATAAGCATTGGAAGCCTCGGAGGGCATCGCGATGAACAACCTGTAACTGGCGTCATAATCAAGAACGTAACATTCAATAATACGCAGAATGATGCAAGAATCAAGACTTGGCAGGCCTTTTATGCCGGGCTTGTGTCAGATATACACTATGAGGATATTACCATGGTTAATGTCAGCAACCCGATCATTATAGACCAAGAGTACTGCCCATGGAATATGTGCAACAAGCAGATTCCTTCAAAAGTTAAGATCAGTAATGTGAGCTTCAAGAACATTAAAGGCACGTCCACAAATAATCTTAGCCCGTTAGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

312

Amino Acids

33.96

Weight (kDa)

8.57

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 17 - 304 1.5e-83 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 614
AccII CGCG 2 cut(s) 270, 648
AciI CCGC 3 cut(s) 268, 270, 603
AclI AACGTT 1 cut(s) 411
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 1 cut(s) 584
AcuI CTGAAG 1 cut(s) 131
AcyI GRCGYC 1 cut(s) 669
AfaI GTAC 2 cut(s) 224, 821
AfiI CCNNNNNNNGG 2 cut(s) 614, 665
AgsI TTSAA 6 cut(s) 143, 409, 504, 694, 858, 886
AjiI CACGTC 2 cut(s) 195, 903
AjnI CCWGG 1 cut(s) 607
AjuI GAANNNNNNNTTGG 2 cut(s) 709, 741
AluBI AGCT 2 cut(s) 35, 882
AluI AGCT 2 cut(s) 35, 882
Alw21I GWGCWC 1 cut(s) 277
Alw26I GTCTC 1 cut(s) 537
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 4 cut(s) 3, 265, 610, 732
ApoI RAATTY 1 cut(s) 584
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
AspS9I GGNCC 3 cut(s) 4, 14, 605
AsuC2I CCSGG 2 cut(s) 462, 745
AsuHPI GGTGA 4 cut(s) 48, 189, 229, 575
AvaI CYCGRG 1 cut(s) 324
AvaII GGWCC 2 cut(s) 14, 605
BaeI ACNNNNGTAYC 2 cut(s) 195, 228
BanI GGYRCC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 277
BccI CCATC 5 cut(s) 257, 458, 502, 560, 566
BciT130I CCWGG 1 cut(s) 609
BciVI GTATCC 2 cut(s) 196, 497
BcnI CCSGG 2 cut(s) 462, 745
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 1 cut(s) 183
BfuI GTATCC 2 cut(s) 196, 497
BisI GCNGC 1 cut(s) 268
BlsI GCNGC 1 cut(s) 269
BmcAI AGTACT 1 cut(s) 821
Bme1390I CCNGG 3 cut(s) 462, 609, 745
Bme18I GGWCC 2 cut(s) 14, 605
BmeT110I CYCGRG 1 cut(s) 324
BmgBI CACGTC 2 cut(s) 195, 903
BmgT120I GGNCC 3 cut(s) 4, 14, 605
BmiI GGNNCC 5 cut(s) 6, 16, 91, 135, 538
BmrFI CCNGG 3 cut(s) 462, 609, 745
BmsI GCATC 5 cut(s) 34, 62, 540, 652, 700
BpuMI CCSGG 2 cut(s) 462, 745
BsaBI GATNNNNATC 1 cut(s) 557
BsaHI GRCGYC 1 cut(s) 669
BsaJI CCNNGG 6 cut(s) 59, 268, 613, 634, 780, 827
BsaWI WCCGGW 2 cut(s) 199, 539
Bsc4I CCNNNNNNNGG 2 cut(s) 614, 665
Bse1I ACTGG 2 cut(s) 16, 670
Bse3DI GCAATG 2 cut(s) 124, 492
Bse8I GATNNNNATC 1 cut(s) 557
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 6 cut(s) 59, 268, 613, 634, 780, 827
BseJI GATNNNNATC 1 cut(s) 557
BseLI CCNNNNNNNGG 2 cut(s) 614, 665
BseMI GCAATG 2 cut(s) 124, 492
BseNI ACTGG 2 cut(s) 16, 670
Bsh1236I CGCG 2 cut(s) 270, 648
BshFI GGCC 4 cut(s) 5, 267, 612, 734
BshNI GGYRCC 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 324
BsiSI CCGG 4 cut(s) 200, 461, 540, 744
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 2 cut(s) 614, 665
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 1 cut(s) 583
BsnI GGCC 4 cut(s) 5, 267, 612, 734
BsoBI CYCGRG 1 cut(s) 324
Bsp1286I GDGCHC 1 cut(s) 277
Bsp143I GATC 2 cut(s) 801, 867
Bsp19I CCATGG 3 cut(s) 613, 780, 827
Bsp68I TCGCGA 1 cut(s) 648
BspACI CCGC 3 cut(s) 268, 270, 603
BspANI GGCC 4 cut(s) 5, 267, 612, 734
BspFNI CGCG 2 cut(s) 270, 648
BspLI GGNNCC 5 cut(s) 6, 16, 91, 135, 538
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 2 cut(s) 124, 492
BsrI ACTGG 2 cut(s) 16, 670
BssECI CCNNGG 6 cut(s) 59, 268, 613, 634, 780, 827
BssMI GATC 2 cut(s) 801, 867
BssNI GRCGYC 1 cut(s) 669
BssT1I CCWWGG 3 cut(s) 613, 780, 827
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 2 cut(s) 299, 517
BstACI GRCGYC 1 cut(s) 669
BstC8I GCNNGC 3 cut(s) 168, 421, 732
BstDEI CTNAG 1 cut(s) 917
BstDSI CCRYGG 4 cut(s) 268, 613, 780, 827
BstFNI CGCG 2 cut(s) 270, 648
BstKTI GATC 2 cut(s) 804, 870
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 2 cut(s) 801, 867
BstMWI GCNNNNNNNGC 3 cut(s) 609, 630, 740
BstNI CCWGG 1 cut(s) 609
BstNSI RCATGY 2 cut(s) 51, 446
BstSCI CCNGG 3 cut(s) 460, 607, 743
BstUI CGCG 2 cut(s) 270, 648
BsuI GTATCC 2 cut(s) 196, 497
BsuRI GGCC 4 cut(s) 5, 267, 612, 734
BtgI CCRYGG 4 cut(s) 268, 613, 780, 827
BtgZI GCGATG 2 cut(s) 629, 663
BtrI CACGTC 2 cut(s) 195, 903
BtsIMutI CAGTG 2 cut(s) 9, 513
BtuMI TCGCGA 1 cut(s) 648
Cac8I GCNNGC 3 cut(s) 168, 421, 732
Cfr13I GGNCC 3 cut(s) 4, 14, 605
Cfr42I CCGCGG 1 cut(s) 271
CseI GACGC 1 cut(s) 658
Csp6I GTAC 2 cut(s) 223, 820
CviAII CATG 5 cut(s) 48, 443, 614, 781, 828
CviQI GTAC 2 cut(s) 223, 820
DdeI CTNAG 1 cut(s) 917
DpnI GATC 2 cut(s) 803, 869
DpnII GATC 2 cut(s) 801, 867
EaeI YGGCCR 1 cut(s) 265
Eco130I CCWWGG 3 cut(s) 613, 780, 827
Eco147I AGGCCT 1 cut(s) 734
Eco47I GGWCC 2 cut(s) 14, 605
Eco57I CTGAAG 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 3 cut(s) 613, 780, 827
EcoT22I ATGCAT 1 cut(s) 49
ErhI CCWWGG 3 cut(s) 613, 780, 827
FaeI CATG 5 cut(s) 51, 446, 617, 784, 831
FaqI GGGAC 1 cut(s) 583
FatI CATG 5 cut(s) 47, 442, 613, 780, 827
Fnu4HI GCNGC 1 cut(s) 268
Fsp4HI GCNGC 1 cut(s) 268
FspBI CTAG 1 cut(s) 183
GluI GCNGC 1 cut(s) 268
HaeIII GGCC 4 cut(s) 5, 267, 612, 734
HapII CCGG 4 cut(s) 200, 461, 540, 744
HgaI GACGC 1 cut(s) 658
Hin1I GRCGYC 1 cut(s) 669
Hin1II CATG 5 cut(s) 51, 446, 617, 784, 831
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 33
HinfI GANTC 4 cut(s) 189, 717, 850, 929
HpaII CCGG 4 cut(s) 200, 461, 540, 744
HphI GGTGA 4 cut(s) 48, 189, 229, 575
Hpy166II GTNNAC 3 cut(s) 334, 355, 906
Hpy188I TCNGA 3 cut(s) 536, 638, 757
Hpy188III TCNNGA 6 cut(s) 331, 647, 679, 721, 886, 933
Hpy8I GTNNAC 3 cut(s) 334, 355, 906
HpyAV CCTTC 2 cut(s) 476, 864
HpyCH4III ACNGT 2 cut(s) 299, 517
HpyCH4IV ACGT 6 cut(s) 194, 411, 499, 594, 684, 902
HpyCH4V TGCA 9 cut(s) 47, 53, 129, 166, 318, 446, 497, 713, 840
HpyF10VI GCNNNNNNNGC 3 cut(s) 609, 630, 740
HpyF3I CTNAG 1 cut(s) 917
HpySE526I ACGT 6 cut(s) 194, 411, 499, 594, 684, 902
Hsp92I GRCGYC 1 cut(s) 669
Hsp92II CATG 5 cut(s) 51, 446, 617, 784, 831
KspI CCGCGG 1 cut(s) 271
Kzo9I GATC 2 cut(s) 801, 867
LmnI GCTCC 3 cut(s) 107, 139, 272
LweI GCATC 5 cut(s) 34, 62, 540, 652, 700
MaeI CTAG 1 cut(s) 183
MaeII ACGT 6 cut(s) 194, 411, 499, 594, 684, 902
MaeIII GTNAC 7 cut(s) 190, 195, 299, 511, 595, 661, 685
MalI GATC 2 cut(s) 803, 869
MboI GATC 2 cut(s) 801, 867
MfeI CAATTG 1 cut(s) 547
MhlI GDGCHC 1 cut(s) 277
MluCI AATT 4 cut(s) 111, 366, 547, 584
MlyI GAGTC 1 cut(s) 198
MmeI TCCRAC 1 cut(s) 463
MnlI CCTC 7 cut(s) 54, 80, 113, 149, 632, 644, 763
Mph1103I ATGCAT 1 cut(s) 49
MseI TTAA 8 cut(s) 101, 228, 369, 474, 582, 786, 864, 894
MslI CAYNNNNRTG 1 cut(s) 648
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 4 cut(s) 200, 461, 540, 744
MspR9I CCNGG 3 cut(s) 462, 609, 745
MunI CAATTG 1 cut(s) 547
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 2 cut(s) 270, 648
MwoI GCNNNNNNNGC 3 cut(s) 609, 630, 740
NciI CCSGG 2 cut(s) 462, 745
NcoI CCATGG 3 cut(s) 613, 780, 827
NdeII GATC 2 cut(s) 801, 867
NlaIII CATG 5 cut(s) 51, 446, 617, 784, 831
NlaIV GGNNCC 5 cut(s) 6, 16, 91, 135, 538
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 4 cut(s) 190, 195, 511, 595
NruI TCGCGA 1 cut(s) 648
NsiI ATGCAT 1 cut(s) 49
NspI RCATGY 2 cut(s) 51, 446
PceI AGGCCT 1 cut(s) 734
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 3 cut(s) 717, 850, 929
PflFI GACNNNGTC 1 cut(s) 350
PflMI CCANNNNNTGG 1 cut(s) 614
PkrI GCNGC 1 cut(s) 269
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Psp1406I AACGTT 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 5 cut(s) 6, 16, 91, 135, 538
PspPI GGNCC 3 cut(s) 4, 14, 605
PsyI GACNNNGTC 1 cut(s) 350
RruI TCGCGA 1 cut(s) 648
RsaI GTAC 2 cut(s) 224, 821
RsaNI GTAC 2 cut(s) 223, 820
RseI CAYNNNNRTG 1 cut(s) 648
SacII CCGCGG 1 cut(s) 271
SaqAI TTAA 8 cut(s) 101, 228, 369, 474, 582, 786, 864, 894
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 2 cut(s) 801, 867
Sau96I GGNCC 3 cut(s) 4, 14, 605
ScaI AGTACT 1 cut(s) 821
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 3 cut(s) 462, 609, 745
SduI GDGCHC 1 cut(s) 277
SfaNI GCATC 5 cut(s) 34, 62, 540, 652, 700
Sfr303I CCGCGG 1 cut(s) 271
SgrBI CCGCGG 1 cut(s) 271
SinI GGWCC 2 cut(s) 14, 605
SmiMI CAYNNNNRTG 1 cut(s) 648
Sse9I AATT 4 cut(s) 111, 366, 547, 584
SseBI AGGCCT 1 cut(s) 734
SsiI CCGC 3 cut(s) 268, 270, 603
SspMI CTAG 1 cut(s) 183
StuI AGGCCT 1 cut(s) 734
StyD4I CCNGG 3 cut(s) 460, 607, 743
StyI CCWWGG 3 cut(s) 613, 780, 827
TaaI ACNGT 2 cut(s) 299, 517
TaiI ACGT 6 cut(s) 197, 414, 502, 597, 687, 905
TaqI TCGA 2 cut(s) 154, 345
TasI AATT 4 cut(s) 111, 366, 547, 584
TatI WGTACW 1 cut(s) 819
TauI GCSGC 1 cut(s) 270
TfiI GAWTC 3 cut(s) 717, 850, 929
Tru1I TTAA 8 cut(s) 101, 228, 369, 474, 582, 786, 864, 894
Tru9I TTAA 8 cut(s) 101, 228, 369, 474, 582, 786, 864, 894
TscAI CASTG 2 cut(s) 16, 520
TseFI GTSAC 4 cut(s) 190, 195, 511, 595
Tsp45I GTSAC 4 cut(s) 190, 195, 511, 595
TspDTI ATGAA 1 cut(s) 666
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 2 cut(s) 16, 520
Tth111I GACNNNGTC 1 cut(s) 350
Van91I CCANNNNNTGG 1 cut(s) 614
VpaK11BI GGWCC 2 cut(s) 14, 605
XapI RAATTY 1 cut(s) 584
XceI RCATGY 2 cut(s) 51, 446
XspI CTAG 1 cut(s) 183
ZrmI AGTACT 1 cut(s) 821
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.