MD09G1120300.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000434
Physical Location & Seq
Reverse (-)
9315252 .. 9317575
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1120300.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGATTTGAAATTGAAAAGTATCTTGCCCATGTTTGTGTTCATGTTGTTAGTATCCACATCTAACGCCAAATCCAGTATCTATGATGTCACTAGTGCAAAATATGGTGGAAAGGCAAACTCTGATATATCCCAGGCTTTTAGCAAAGCTTGGACAGATGCATGTGCATCGACATTGGCAAGTAAAATTGTTGTTCCAAGAGGGACATACAAGTTTGTAGGGGCAACTTTCAAAGGACCATGCAAAGCTCCTATTGAGATTCAGCTCCAAGGCACACTGCAGGCTCCAGCAGATGGCAGCAAACTCCCAAAAGCAGATACCTGGGTAGGTTTTGAGCGCATTGATGGGCTCACCTTATCCGGTGGTGGAACTTTCGATGGCCAAGGAGCAACTGCTTGGAAGCAAAATGATTGCAACAAAAACAAAAATTGCAAATCTATTGCTATCAATCTGAGGTTCAATTTTGTCACCAATTCCATAATTCGGGACATAACAACAAAAGACAGCAAAAATTTCCACGTCAATGTTTTGGGTTGCAACAATGTGGTATTCCAACATTTTACCGTCTCAGCGCCTAAAGAGAGCATGAACACAGATGGAATTCATATCGGACGTTCAACTGGGATCAACATCACAGATTCAACCATTGGAACAGGGGATGATTGCATCTCTATAGGTGATGGCACCAAGCAACTCCATGTAACCAAAGTTACTTGCGGACCCGGCCATGGCATAAGCATCGGGAGTCTCGGAAGATATCAGAATGAAGAACCTGTGTCCGGAATCTTTATCAAGAACTGCACAATTAAAAATACAGATAATGGTGTGAGAATCAAAACATGGCCTGCTTCTCCATCAAATGGTGTAGTCTCAGATGTTCACTTTGAGGATATTATTATGGACAATGTTGGAAACCCTGTCATCATAGACCAAGAGTATTGTCCATGGAATCAGTGCACACTTAAGGTTCCATCCAAAGTTAAGATCAGCAATGTCAGCTTCAAAAACATAAAGGGCTCATCTTCAACCCCAGTCGGTATTAAGATTGTATGCAGTGGAGGGCTGCCATGTGAGAATGTGCAACTAAGTGACATTGATCTCACATACAGTGGAAACAAAGGCCCTATTACCTCTCAATGTAAGCATGTCAAGCCCACCATTAACAACGTGGCACGGGCTCTTGCTTGCGCGACCTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

401

Amino Acids

42.69

Weight (kDa)

8.9

Isoelectric Point (pI)

25.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 384 1.1e-104 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 683
AccB7I CCANNNNNTGG 2 cut(s) 293, 860
AccII CGCG 1 cut(s) 1190
AccIII TCCGGA 1 cut(s) 779
AciI CCGC 1 cut(s) 717
AclWI GGATC 1 cut(s) 632
AcoI YGGCCR 2 cut(s) 379, 724
AcsI RAATTY 2 cut(s) 511, 600
AfiI CCNNNNNNNGG 5 cut(s) 293, 483, 728, 779, 860
AflII CTTAAG 1 cut(s) 962
AgsI TTSAA 8 cut(s) 10, 16, 232, 460, 618, 642, 1003, 1026
AhlI ACTAGT 1 cut(s) 92
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 2 cut(s) 132, 320
AluBI AGCT 4 cut(s) 149, 248, 265, 999
AluI AGCT 4 cut(s) 149, 248, 265, 999
Alw21I GWGCWC 1 cut(s) 959
Alw26I GTCTC 3 cut(s) 571, 752, 874
Alw44I GTGCAC 1 cut(s) 955
AlwI GGATC 1 cut(s) 632
Aor13HI TCCGGA 1 cut(s) 779
AoxI GGCC 4 cut(s) 379, 724, 842, 1120
ApaLI GTGCAC 1 cut(s) 955
ApeKI GCWGC 2 cut(s) 297, 1063
ApoI RAATTY 2 cut(s) 511, 600
AspLEI GCGC 3 cut(s) 339, 574, 1190
AspS9I GGNCC 3 cut(s) 236, 719, 1121
AsuC2I CCSGG 1 cut(s) 723
AsuHPI GGTGA 3 cut(s) 343, 460, 689
AvaII GGWCC 2 cut(s) 236, 719
BaeGI GKGCMC 1 cut(s) 959
BaeI ACNNNNGTAYC 2 cut(s) 309, 342
BalI TGGCCA 1 cut(s) 381
BanI GGYRCC 1 cut(s) 683
BanII GRGCYC 3 cut(s) 351, 1019, 1180
Bbv12I GWGCWC 1 cut(s) 959
BbvI GCAGC 2 cut(s) 309, 1050
BccI CCATC 7 cut(s) 287, 338, 371, 590, 674, 862, 979
BcgI CGANNNNNNTGC 4 cut(s) 150, 184, 721, 755
BciT130I CCWGG 2 cut(s) 134, 322
BciVI GTATCC 1 cut(s) 64
BcnI CCSGG 1 cut(s) 723
BcoDI GTCTC 3 cut(s) 571, 752, 874
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 2 cut(s) 278, 672
BfoI RGCGCY 1 cut(s) 575
BfrI CTTAAG 1 cut(s) 962
BfuI GTATCC 1 cut(s) 64
BisI GCNGC 2 cut(s) 298, 1064
BlsI GCNGC 2 cut(s) 299, 1065
Bme1390I CCNGG 3 cut(s) 134, 322, 723
Bme18I GGWCC 2 cut(s) 236, 719
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 3 cut(s) 236, 719, 1121
BmiI GGNNCC 4 cut(s) 285, 685, 721, 969
BmrFI CCNGG 3 cut(s) 134, 322, 723
BmrI ACTGGG 2 cut(s) 630, 1025
BmsI GCATC 4 cut(s) 148, 176, 675, 747
BmuI ACTGGG 2 cut(s) 630, 1025
BpmI CTGGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 723
BsaBI GATNNNNATC 1 cut(s) 629
BsaJI CCNNGG 6 cut(s) 132, 268, 321, 382, 727, 944
BsaWI WCCGGW 2 cut(s) 359, 779
Bsc4I CCNNNNNNNGG 5 cut(s) 293, 483, 728, 779, 860
Bse1I ACTGG 3 cut(s) 75, 625, 1031
Bse3DI GCAATG 1 cut(s) 997
Bse8I GATNNNNATC 1 cut(s) 629
BseAI TCCGGA 1 cut(s) 779
BseBI CCWGG 2 cut(s) 134, 322
BseDI CCNNGG 6 cut(s) 132, 268, 321, 382, 727, 944
BseGI GGATG 2 cut(s) 664, 971
BseJI GATNNNNATC 1 cut(s) 629
BseLI CCNNNNNNNGG 5 cut(s) 293, 483, 728, 779, 860
BseMI GCAATG 1 cut(s) 997
BseMII CTCAG 3 cut(s) 443, 582, 885
BseNI ACTGG 3 cut(s) 75, 625, 1031
BseSI GKGCMC 1 cut(s) 959
BseXI GCAGC 2 cut(s) 309, 1050
BsgI GTGCAG 1 cut(s) 784
Bsh1236I CGCG 1 cut(s) 1190
BshFI GGCC 4 cut(s) 381, 726, 844, 1122
BshNI GGYRCC 1 cut(s) 683
BsiHKAI GWGCWC 1 cut(s) 959
BsiSI CCGG 3 cut(s) 360, 723, 780
BslFI GGGAC 2 cut(s) 217, 500
BslI CCNNNNNNNGG 5 cut(s) 293, 483, 728, 779, 860
BsmAI GTCTC 3 cut(s) 571, 752, 874
BsmBI CGTCTC 1 cut(s) 571
BsmFI GGGAC 2 cut(s) 217, 500
BsnI GGCC 4 cut(s) 381, 726, 844, 1122
Bsp1286I GDGCHC 4 cut(s) 351, 959, 1019, 1180
Bsp13I TCCGGA 1 cut(s) 779
Bsp143I GATC 3 cut(s) 624, 984, 1096
Bsp19I CCATGG 2 cut(s) 727, 944
BspACI CCGC 1 cut(s) 717
BspANI GGCC 4 cut(s) 381, 726, 844, 1122
BspCNI CTCAG 3 cut(s) 444, 581, 884
BspEI TCCGGA 1 cut(s) 779
BspFNI CGCG 1 cut(s) 1190
BspLI GGNNCC 4 cut(s) 285, 685, 721, 969
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 1 cut(s) 632
BspT107I GGYRCC 1 cut(s) 683
BspTI CTTAAG 1 cut(s) 962
BsrDI GCAATG 1 cut(s) 997
BsrI ACTGG 3 cut(s) 75, 625, 1031
BssECI CCNNGG 6 cut(s) 132, 268, 321, 382, 727, 944
BssMI GATC 3 cut(s) 624, 984, 1096
BssT1I CCWWGG 4 cut(s) 268, 382, 727, 944
Bst2UI CCWGG 2 cut(s) 134, 322
Bst4CI ACNGT 2 cut(s) 565, 1109
BstAFI CTTAAG 1 cut(s) 962
BstC8I GCNNGC 3 cut(s) 282, 846, 1186
BstDEI CTNAG 4 cut(s) 452, 568, 871, 1085
BstDSI CCRYGG 2 cut(s) 727, 944
BstF5I GGATG 2 cut(s) 664, 971
BstFNI CGCG 1 cut(s) 1190
BstH2I RGCGCY 1 cut(s) 575
BstHHI GCGC 3 cut(s) 339, 574, 1190
BstKTI GATC 3 cut(s) 627, 987, 1099
BstMAI GTCTC 3 cut(s) 571, 752, 874
BstMBI GATC 3 cut(s) 624, 984, 1096
BstMWI GCNNNNNNNGC 3 cut(s) 723, 996, 1150
BstNI CCWGG 2 cut(s) 134, 322
BstNSI RCATGY 2 cut(s) 165, 1148
BstSCI CCNGG 3 cut(s) 132, 320, 721
BstSFI CTRYAG 2 cut(s) 278, 672
BstSLI GKGCMC 1 cut(s) 959
BstUI CGCG 1 cut(s) 1190
BstV1I GCAGC 2 cut(s) 309, 1050
BsuI GTATCC 1 cut(s) 64
BsuRI GGCC 4 cut(s) 381, 726, 844, 1122
BtgI CCRYGG 2 cut(s) 727, 944
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 2 cut(s) 664, 971
BtsI GCAGTG 2 cut(s) 275, 1060
BtsIMutI CAGTG 4 cut(s) 275, 959, 1060, 1114
Cac8I GCNNGC 3 cut(s) 282, 846, 1186
CfoI GCGC 3 cut(s) 339, 574, 1190
Cfr13I GGNCC 3 cut(s) 236, 719, 1121
DdeI CTNAG 4 cut(s) 452, 568, 871, 1085
DpnI GATC 3 cut(s) 626, 986, 1098
DpnII GATC 3 cut(s) 624, 984, 1096
EaeI YGGCCR 2 cut(s) 379, 724
Eco130I CCWWGG 4 cut(s) 268, 382, 727, 944
Eco24I GRGCYC 3 cut(s) 351, 1019, 1180
Eco32I GATATC 1 cut(s) 758
Eco47I GGWCC 2 cut(s) 236, 719
EcoO109I RGGNCCY 1 cut(s) 1121
EcoRI GAATTC 1 cut(s) 600
EcoRII CCWGG 2 cut(s) 132, 320
EcoRV GATATC 1 cut(s) 758
EcoT14I CCWWGG 4 cut(s) 268, 382, 727, 944
EcoT22I ATGCAT 1 cut(s) 163
EcoT38I GRGCYC 3 cut(s) 351, 1019, 1180
ErhI CCWWGG 4 cut(s) 268, 382, 727, 944
Esp3I CGTCTC 1 cut(s) 571
FaqI GGGAC 2 cut(s) 217, 500
Fnu4HI GCNGC 2 cut(s) 298, 1064
FokI GGATG 2 cut(s) 671, 958
FriOI GRGCYC 3 cut(s) 351, 1019, 1180
Fsp4HI GCNGC 2 cut(s) 298, 1064
FspBI CTAG 1 cut(s) 93
GlaI GCGC 3 cut(s) 338, 573, 1189
GluI GCNGC 2 cut(s) 298, 1064
GsuI CTGGAG 1 cut(s) 270
HaeII RGCGCY 1 cut(s) 575
HaeIII GGCC 4 cut(s) 381, 726, 844, 1122
HapII CCGG 3 cut(s) 360, 723, 780
HhaI GCGC 3 cut(s) 339, 574, 1190
Hin6I GCGC 3 cut(s) 337, 572, 1188
HinP1I GCGC 3 cut(s) 337, 572, 1188
HindIII AAGCTT 1 cut(s) 147
HinfI GANTC 6 cut(s) 259, 638, 745, 783, 831, 949
HpaII CCGG 3 cut(s) 360, 723, 780
HphI GGTGA 3 cut(s) 343, 460, 689
Hpy166II GTNNAC 2 cut(s) 880, 957
Hpy188I TCNGA 6 cut(s) 124, 453, 611, 752, 762, 874
Hpy188III TCNNGA 5 cut(s) 485, 742, 780, 793, 1200
Hpy8I GTNNAC 2 cut(s) 880, 957
HpyCH4III ACNGT 2 cut(s) 565, 1109
HpyCH4IV ACGT 3 cut(s) 519, 613, 1167
HpyF10VI GCNNNNNNNGC 3 cut(s) 723, 996, 1150
HpyF3I CTNAG 4 cut(s) 452, 568, 871, 1085
HpySE526I ACGT 3 cut(s) 519, 613, 1167
HspAI GCGC 3 cut(s) 337, 572, 1188
Kpn2I TCCGGA 1 cut(s) 779
Kzo9I GATC 3 cut(s) 624, 984, 1096
LmnI GCTCC 4 cut(s) 253, 270, 289, 386
Lsp1109I GCAGC 2 cut(s) 309, 1050
LweI GCATC 4 cut(s) 148, 176, 675, 747
MaeI CTAG 1 cut(s) 93
MaeII ACGT 3 cut(s) 519, 613, 1167
MaeIII GTNAC 5 cut(s) 88, 466, 700, 709, 1088
MalI GATC 3 cut(s) 626, 986, 1098
MboI GATC 3 cut(s) 624, 984, 1096
MboII GAAGA 4 cut(s) 765, 779, 1014, 1188
MhlI GDGCHC 4 cut(s) 351, 959, 1019, 1180
MlsI TGGCCA 1 cut(s) 381
MluCI AATT 9 cut(s) 11, 186, 427, 460, 472, 480, 511, 600, 804
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 1 cut(s) 754
MmeI TCCRAC 2 cut(s) 577, 889
MnlI CCTC 5 cut(s) 194, 447, 880, 1052, 1141
Mox20I TGGCCA 1 cut(s) 381
Mph1103I ATGCAT 1 cut(s) 163
MroI TCCGGA 1 cut(s) 779
MscI TGGCCA 1 cut(s) 381
MseI TTAA 5 cut(s) 807, 963, 981, 1041, 1161
MslI CAYNNNNRTG 2 cut(s) 35, 522
Msp20I TGGCCA 1 cut(s) 381
MspCI CTTAAG 1 cut(s) 962
MspI CCGG 3 cut(s) 360, 723, 780
MspR9I CCNGG 3 cut(s) 134, 322, 723
MvaI CCWGG 2 cut(s) 134, 322
MvnI CGCG 1 cut(s) 1190
MwoI GCNNNNNNNGC 3 cut(s) 723, 996, 1150
NciI CCSGG 1 cut(s) 723
NcoI CCATGG 2 cut(s) 727, 944
NdeII GATC 3 cut(s) 624, 984, 1096
NlaIV GGNNCC 4 cut(s) 285, 685, 721, 969
NmuCI GTSAC 3 cut(s) 88, 466, 1088
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 2 cut(s) 165, 1148
PfeI GAWTC 5 cut(s) 259, 638, 783, 831, 949
PflMI CCANNNNNTGG 2 cut(s) 293, 860
PkrI GCNGC 2 cut(s) 299, 1065
PleI GAGTC 1 cut(s) 753
PpsI GAGTC 1 cut(s) 753
Psp6I CCWGG 2 cut(s) 132, 320
PspGI CCWGG 2 cut(s) 132, 320
PspN4I GGNNCC 4 cut(s) 285, 685, 721, 969
PspPI GGNCC 3 cut(s) 236, 719, 1121
PstI CTGCAG 1 cut(s) 282
RseI CAYNNNNRTG 2 cut(s) 35, 522
SaqAI TTAA 5 cut(s) 807, 963, 981, 1041, 1161
SatI GCNGC 2 cut(s) 298, 1064
Sau3AI GATC 3 cut(s) 624, 984, 1096
Sau96I GGNCC 3 cut(s) 236, 719, 1121
SchI GAGTC 1 cut(s) 754
ScrFI CCNGG 3 cut(s) 134, 322, 723
SduI GDGCHC 4 cut(s) 351, 959, 1019, 1180
SfaNI GCATC 4 cut(s) 148, 176, 675, 747
SfcI CTRYAG 2 cut(s) 278, 672
SinI GGWCC 2 cut(s) 236, 719
SmiMI CAYNNNNRTG 2 cut(s) 35, 522
SmlI CTYRAG 1 cut(s) 962
SmoI CTYRAG 1 cut(s) 962
SpeI ACTAGT 1 cut(s) 92
Sse9I AATT 9 cut(s) 11, 186, 427, 460, 472, 480, 511, 600, 804
SsiI CCGC 1 cut(s) 717
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 3 cut(s) 132, 320, 721
StyI CCWWGG 4 cut(s) 268, 382, 727, 944
TaaI ACNGT 2 cut(s) 565, 1109
TaiI ACGT 3 cut(s) 522, 616, 1170
TaqI TCGA 2 cut(s) 170, 375
TasI AATT 9 cut(s) 11, 186, 427, 460, 472, 480, 511, 600, 804
TfiI GAWTC 5 cut(s) 259, 638, 783, 831, 949
Tru1I TTAA 5 cut(s) 807, 963, 981, 1041, 1161
Tru9I TTAA 5 cut(s) 807, 963, 981, 1041, 1161
TscAI CASTG 4 cut(s) 282, 959, 1060, 1114
TseFI GTSAC 3 cut(s) 88, 466, 1088
TseI GCWGC 2 cut(s) 297, 1063
Tsp45I GTSAC 3 cut(s) 88, 466, 1088
TspDTI ATGAA 4 cut(s) 31, 593, 602, 780
TspRI CASTG 4 cut(s) 282, 959, 1060, 1114
Van91I CCANNNNNTGG 2 cut(s) 293, 860
Vha464I CTTAAG 1 cut(s) 962
VneI GTGCAC 1 cut(s) 955
VpaK11BI GGWCC 2 cut(s) 236, 719
XapI RAATTY 2 cut(s) 511, 600
XceI RCATGY 2 cut(s) 165, 1148
XcmI CCANNNNNNNNNTGG 1 cut(s) 1165
XspI CTAG 1 cut(s) 93
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.