RLG00000030295

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000434
Physical Location & Seq
Reverse (-)
59166307 .. 59172209
5903 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030295

Sequence Viewer

Length: 465 bp
ATGAGGTTCAACTCTCTCAAGAACTCATTAGTCAAGGATGTAACTTCACTTAACAGTAAGAATTTCTACGTGAACGTATTAGCGTGCGAACAACTTACATTCCAACATTTCACTGTCGCTGCGCCAGGAGATAGCGTTAATACAGATGGAATACATATTGGGTGCTCAACCGGGGTCAATATTACTGATTCAAAGATCGGTACCGCAGACGATTGCATTTCAATTGGGGATGGCACCAAGCAACTAACTTTAACTAAGGAAACTTGTGGACAAGGACATGGAATAAGCATAGGAAGTCTAAGAAGGTACCCAAAAGAAGAACCTGCTTCATCAAAAATCAAGATCAGCAATGTGAGCTTCAAGAACCTTAGAGGGACGTCTTCCACTCCAGTGGCCGTGAAGATTGCGTGCGCCAAGGGCTTACCATGTGAGAAGGTGGAGATGACTGACATTAATCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

155

Amino Acids

16.44

Weight (kDa)

8.75

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 1 - 103 1.3e-33 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 108 - 153 4.1e-09 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 380
Acc36I ACCTGC 1 cut(s) 331
Acc65I GGTACC 2 cut(s) 200, 306
AccB1I GGYRCC 3 cut(s) 200, 233, 306
AciI CCGC 1 cut(s) 204
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 61
AcyI GRCGYC 1 cut(s) 377
AfaI GTAC 2 cut(s) 202, 308
AgsI TTSAA 4 cut(s) 10, 192, 222, 361
AjnI CCWGG 1 cut(s) 124
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 1 cut(s) 357
AluI AGCT 1 cut(s) 357
Alw21I GWGCWC 1 cut(s) 167
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 1 cut(s) 61
AseI ATTAAT 1 cut(s) 453
Asp718I GGTACC 2 cut(s) 200, 306
AspLEI GCGC 2 cut(s) 124, 413
AsuC2I CCSGG 1 cut(s) 172
BanI GGYRCC 3 cut(s) 200, 233, 306
BbsI GAAGAC 1 cut(s) 372
Bbv12I GWGCWC 1 cut(s) 167
BbvI GCAGC 1 cut(s) 106
BccI CCATC 2 cut(s) 140, 224
BceAI ACGGC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 126
BcnI CCSGG 1 cut(s) 172
BfuAI ACCTGC 1 cut(s) 331
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
Bme1390I CCNGG 2 cut(s) 126, 172
BmiI GGNNCC 3 cut(s) 202, 235, 308
BmrFI CCNGG 2 cut(s) 126, 172
BpiI GAAGAC 1 cut(s) 372
BpmI CTGGAG 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 171, 414
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bse1I ACTGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 355
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 2 cut(s) 171, 414
BseGI GGATG 2 cut(s) 43, 235
BseMI GCAATG 1 cut(s) 355
BseNI ACTGG 1 cut(s) 389
BseXI GCAGC 1 cut(s) 106
BshFI GGCC 1 cut(s) 395
BshNI GGYRCC 3 cut(s) 200, 233, 306
BsiHKAI GWGCWC 1 cut(s) 167
BsiSI CCGG 1 cut(s) 171
BslFI GGGAC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 388
BsnI GGCC 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 167
Bsp143I GATC 2 cut(s) 195, 342
BspACI CCGC 1 cut(s) 204
BspANI GGCC 1 cut(s) 395
BspLI GGNNCC 3 cut(s) 202, 235, 308
BspMI ACCTGC 1 cut(s) 331
BspT107I GGYRCC 3 cut(s) 200, 233, 306
BsrDI GCAATG 1 cut(s) 355
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 2 cut(s) 171, 414
BssMI GATC 2 cut(s) 195, 342
BssNI GRCGYC 1 cut(s) 377
BssT1I CCWWGG 1 cut(s) 414
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 56, 115
BstACI GRCGYC 1 cut(s) 377
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 2 cut(s) 85, 409
BstDEI CTNAG 3 cut(s) 255, 299, 368
BstF5I GGATG 2 cut(s) 43, 235
BstHHI GCGC 2 cut(s) 124, 413
BstKTI GATC 2 cut(s) 198, 345
BstMBI GATC 2 cut(s) 195, 342
BstMWI GCNNNNNNNGC 2 cut(s) 354, 417
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 2 cut(s) 124, 170
BstV1I GCAGC 1 cut(s) 106
BstV2I GAAGAC 1 cut(s) 372
BstXI CCANNNNNNTGG 1 cut(s) 391
BsuRI GGCC 1 cut(s) 395
BtsCI GGATG 2 cut(s) 43, 235
BtsIMutI CAGTG 2 cut(s) 111, 396
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 2 cut(s) 85, 409
CfoI GCGC 2 cut(s) 124, 413
Csp6I GTAC 2 cut(s) 201, 307
CviAII CATG 2 cut(s) 278, 426
CviJI RGCY 3 cut(s) 357, 395, 420
CviKI_1 RGCY 3 cut(s) 357, 395, 420
CviQI GTAC 2 cut(s) 201, 307
DdeI CTNAG 3 cut(s) 255, 299, 368
DpnI GATC 2 cut(s) 197, 344
DpnII GATC 2 cut(s) 195, 342
EaeI YGGCCR 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 414
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 2 cut(s) 281, 429
FaiI YATR 4 cut(s) 156, 279, 290, 427
FaqI GGGAC 1 cut(s) 388
FatI CATG 2 cut(s) 277, 425
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 2 cut(s) 50, 242
Fsp4HI GCNGC 1 cut(s) 120
GlaI GCGC 2 cut(s) 123, 412
GluI GCNGC 1 cut(s) 120
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 1 cut(s) 395
HapII CCGG 1 cut(s) 171
HhaI GCGC 2 cut(s) 124, 413
Hin1I GRCGYC 1 cut(s) 377
Hin1II CATG 2 cut(s) 281, 429
Hin6I GCGC 2 cut(s) 122, 411
HinP1I GCGC 2 cut(s) 122, 411
HinfI GANTC 1 cut(s) 188
HpaII CCGG 1 cut(s) 171
Hpy166II GTNNAC 2 cut(s) 73, 269
Hpy188III TCNNGA 3 cut(s) 19, 340, 361
Hpy8I GTNNAC 2 cut(s) 73, 269
HpyAV CCTTC 2 cut(s) 297, 427
HpyCH4III ACNGT 2 cut(s) 56, 115
HpyCH4IV ACGT 3 cut(s) 69, 75, 377
HpyCH4V TGCA 1 cut(s) 216
HpyF10VI GCNNNNNNNGC 2 cut(s) 354, 417
HpyF3I CTNAG 3 cut(s) 255, 299, 368
HpySE526I ACGT 3 cut(s) 69, 75, 377
Hsp92I GRCGYC 1 cut(s) 377
Hsp92II CATG 2 cut(s) 281, 429
HspAI GCGC 2 cut(s) 122, 411
KpnI GGTACC 2 cut(s) 204, 310
Kzo9I GATC 2 cut(s) 195, 342
LpnPI CCDG 5 cut(s) 111, 138, 184, 336, 402
Lsp1109I GCAGC 1 cut(s) 106
MaeII ACGT 3 cut(s) 69, 75, 377
MaeIII GTNAC 1 cut(s) 40
MalI GATC 2 cut(s) 197, 344
MboI GATC 2 cut(s) 195, 342
MboII GAAGA 3 cut(s) 329, 372, 412
MfeI CAATTG 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 167
MluCI AATT 2 cut(s) 61, 222
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 1 cut(s) 365
MseI TTAA 4 cut(s) 51, 138, 251, 453
MslI CAYNNNNRTG 1 cut(s) 389
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 2 cut(s) 126, 172
MunI CAATTG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 354, 417
NciI CCSGG 1 cut(s) 172
NdeII GATC 2 cut(s) 195, 342
NlaIII CATG 2 cut(s) 281, 429
NlaIV GGNNCC 3 cut(s) 202, 235, 308
OliI CACNNNNGTG 1 cut(s) 389
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 1 cut(s) 121
Ppu21I YACGTR 1 cut(s) 70
PshBI ATTAAT 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 3 cut(s) 202, 235, 308
RsaI GTAC 2 cut(s) 202, 308
RsaNI GTAC 2 cut(s) 201, 307
RseI CAYNNNNRTG 1 cut(s) 389
SaqAI TTAA 4 cut(s) 51, 138, 251, 453
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 2 cut(s) 195, 342
ScrFI CCNGG 2 cut(s) 126, 172
SduI GDGCHC 1 cut(s) 167
SetI ASST 9 cut(s) 8, 72, 78, 308, 325, 359, 369, 380, 438
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 2 cut(s) 17, 458
SmoI CTYRAG 2 cut(s) 17, 458
Sse9I AATT 2 cut(s) 61, 222
SsiI CCGC 1 cut(s) 204
SspI AATATT 1 cut(s) 181
StyD4I CCNGG 2 cut(s) 124, 170
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 56, 115
TaiI ACGT 3 cut(s) 72, 78, 380
TasI AATT 2 cut(s) 61, 222
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 4 cut(s) 51, 138, 251, 453
Tru9I TTAA 4 cut(s) 51, 138, 251, 453
TscAI CASTG 2 cut(s) 118, 396
TseI GCWGC 1 cut(s) 119
TspDTI ATGAA 1 cut(s) 318
TspRI CASTG 2 cut(s) 118, 396
VspI ATTAAT 1 cut(s) 453
XapI RAATTY 1 cut(s) 61
ZraI GACGTC 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.