pycom09g04490

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000434
Physical Location & Seq
Reverse (-)
3326073 .. 3327193
1121 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g04490.1

Sequence Viewer

Length: 786 bp
ATGTTTTTAACTTTTTCACACTATTCACAGAATCTGAGGTTCAATTTTGTCACCAATTCCATAATTCGAGACATAACAACAAAGGACAGCAAAAATTTCCACGTAAATCTTTTGGGTTGCAACAATGTGGCATTCCAACATTTTACCGTCTCAGCGCCTAAAGAGAGCATCAATACCGATGGAATTCATATCGGACGTTCAACTGGGATCAACATCACAGATACGACCATTGGAACTGGGGATGATTGCATCTCTATTGGTGATGGCACCAAGCAACTCCATGTAACCAACGTTACTTGTGGACCAGGCCACGGCATAAGCATCGGGAGCCTCGGAAAGTACGAGAATGAAGAACCTGTGTCCGGAATATTTATCAAGAACTGCACAATTTCAAATACAGATAATGGTGTGAGGATCAAAACATGGCCTGCTTCCCCTGCAAATGGCGTTGTCTCAGATGTTCACTTTGAGGATATTATCATGGATAATGTTAAAAACCCTGTCATCATAGACCAAGAGTATTGCCCACATAATCAGTGCACACTTCAGGTTCCATCCAAAGTTAAGATCAGCAATGTTAGCTTCAAGAACATAAAGGGTTCATCTTCAACCCCAGTTGGTATCAAGCTTGTATGCAGTGGAGGGCTGCCATGTGAGAATGTGGAACTGAGTAACATTGATCTCACATACACCGGAAAAGAAGGCTCTATTACGTCTGAATGTAAACATATCAAGCCCACCATTAACAACGTGGCGCAGCCTCTTGCTTGTGCTACATCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

262

Amino Acids

28.07

Weight (kDa)

6.29

Isoelectric Point (pI)

33.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 10 - 245 2.1e-81 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AccIII TCCGGA 1 cut(s) 362
AclI AACGTT 1 cut(s) 291
AclWI GGATC 2 cut(s) 215, 422
AcsI RAATTY 2 cut(s) 94, 183
AcuI CTGAAG 1 cut(s) 530
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 3 cut(s) 311, 362, 443
AgsI TTSAA 5 cut(s) 43, 201, 393, 586, 609
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 2 cut(s) 582, 628
AluI AGCT 2 cut(s) 582, 628
Alw21I GWGCWC 1 cut(s) 542
Alw26I GTCTC 3 cut(s) 63, 154, 457
Alw44I GTGCAC 1 cut(s) 538
AlwI GGATC 2 cut(s) 215, 422
AlwNI CAGNNNCTG 1 cut(s) 34
Aor13HI TCCGGA 1 cut(s) 362
AoxI GGCC 2 cut(s) 307, 425
ApaLI GTGCAC 1 cut(s) 538
ApeKI GCWGC 2 cut(s) 646, 757
ApoI RAATTY 2 cut(s) 94, 183
AspLEI GCGC 2 cut(s) 157, 757
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 43, 272
AvaII GGWCC 1 cut(s) 302
BaeGI GKGCMC 1 cut(s) 542
BanI GGYRCC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 542
BbvI GCAGC 2 cut(s) 633, 769
BccI CCATC 3 cut(s) 173, 257, 562
BceAI ACGGC 1 cut(s) 328
BcgI CGANNNNNNTGC 2 cut(s) 304, 338
BciT130I CCWGG 1 cut(s) 306
BcoDI GTCTC 3 cut(s) 63, 154, 457
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 2 cut(s) 647, 758
BlsI GCNGC 2 cut(s) 648, 759
Bme1390I CCNGG 1 cut(s) 306
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 3 cut(s) 268, 329, 552
BmrFI CCNGG 1 cut(s) 306
BmrI ACTGGG 3 cut(s) 213, 246, 608
BmsI GCATC 3 cut(s) 177, 258, 330
BmuI ACTGGG 3 cut(s) 213, 246, 608
BsaAI YACGTR 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 310, 331
BsaWI WCCGGW 2 cut(s) 362, 692
Bsc4I CCNNNNNNNGG 3 cut(s) 311, 362, 443
Bse1I ACTGG 3 cut(s) 208, 241, 614
Bse3DI GCAATG 1 cut(s) 580
Bse8I GATNNNNATC 1 cut(s) 212
BseAI TCCGGA 1 cut(s) 362
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 2 cut(s) 310, 331
BseGI GGATG 2 cut(s) 247, 554
BseJI GATNNNNATC 1 cut(s) 212
BseLI CCNNNNNNNGG 3 cut(s) 311, 362, 443
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 4 cut(s) 26, 165, 468, 659
BseNI ACTGG 3 cut(s) 208, 241, 614
BseSI GKGCMC 1 cut(s) 542
BseXI GCAGC 2 cut(s) 633, 769
BsgI GTGCAG 1 cut(s) 367
BshFI GGCC 2 cut(s) 309, 427
BshNI GGYRCC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 542
BsiSI CCGG 2 cut(s) 363, 693
BslI CCNNNNNNNGG 3 cut(s) 311, 362, 443
BsmAI GTCTC 3 cut(s) 63, 154, 457
BsmBI CGTCTC 1 cut(s) 154
BsmI GAATGC 1 cut(s) 131
BsnI GGCC 2 cut(s) 309, 427
Bsp1286I GDGCHC 1 cut(s) 542
Bsp13I TCCGGA 1 cut(s) 362
Bsp143I GATC 4 cut(s) 207, 414, 567, 679
BspANI GGCC 2 cut(s) 309, 427
BspCNI CTCAG 4 cut(s) 27, 164, 467, 660
BspEI TCCGGA 1 cut(s) 362
BspLI GGNNCC 3 cut(s) 268, 329, 552
BspPI GGATC 2 cut(s) 215, 422
BspT107I GGYRCC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 580
BsrI ACTGG 3 cut(s) 208, 241, 614
BssECI CCNNGG 2 cut(s) 310, 331
BssMI GATC 4 cut(s) 207, 414, 567, 679
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 148
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 4 cut(s) 35, 151, 454, 668
BstDSI CCRYGG 1 cut(s) 310
BstF5I GGATG 2 cut(s) 247, 554
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 2 cut(s) 157, 757
BstKTI GATC 4 cut(s) 210, 417, 570, 682
BstMAI GTCTC 3 cut(s) 63, 154, 457
BstMBI GATC 4 cut(s) 207, 414, 567, 679
BstMWI GCNNNNNNNGC 3 cut(s) 327, 437, 579
BstNI CCWGG 1 cut(s) 306
BstSCI CCNGG 1 cut(s) 304
BstSLI GKGCMC 1 cut(s) 542
BstV1I GCAGC 2 cut(s) 633, 769
BsuRI GGCC 2 cut(s) 309, 427
BtgI CCRYGG 1 cut(s) 310
BtsCI GGATG 2 cut(s) 247, 554
BtsI GCAGTG 1 cut(s) 643
BtsIMutI CAGTG 2 cut(s) 542, 643
Cac8I GCNNGC 1 cut(s) 429
CaiI CAGNNNCTG 1 cut(s) 34
CfoI GCGC 2 cut(s) 157, 757
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 340
CviAII CATG 4 cut(s) 281, 423, 481, 651
CviJI RGCY 9 cut(s) 309, 330, 427, 582, 628, 646, 705, 736, 760
CviKI_1 RGCY 9 cut(s) 309, 330, 427, 582, 628, 646, 705, 736, 760
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 4 cut(s) 35, 151, 454, 668
DpnI GATC 4 cut(s) 209, 416, 569, 681
DpnII GATC 4 cut(s) 207, 414, 567, 679
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 304
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 4 cut(s) 284, 426, 484, 654
FatI CATG 4 cut(s) 280, 422, 480, 650
Fnu4HI GCNGC 2 cut(s) 647, 758
FokI GGATG 2 cut(s) 254, 541
Fsp4HI GCNGC 2 cut(s) 647, 758
GlaI GCGC 2 cut(s) 156, 756
GluI GCNGC 2 cut(s) 647, 758
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 2 cut(s) 309, 427
HapII CCGG 2 cut(s) 363, 693
HhaI GCGC 2 cut(s) 157, 757
Hin1II CATG 4 cut(s) 284, 426, 484, 654
Hin6I GCGC 2 cut(s) 155, 755
HinP1I GCGC 2 cut(s) 155, 755
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 1 cut(s) 31
HpaII CCGG 2 cut(s) 363, 693
HphI GGTGA 2 cut(s) 43, 272
Hpy166II GTNNAC 4 cut(s) 302, 463, 540, 725
Hpy188I TCNGA 5 cut(s) 36, 194, 335, 457, 718
Hpy188III TCNNGA 6 cut(s) 68, 325, 363, 376, 586, 783
Hpy8I GTNNAC 4 cut(s) 302, 463, 540, 725
HpyAV CCTTC 1 cut(s) 695
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 5 cut(s) 102, 196, 291, 713, 750
HpyCH4V TGCA 6 cut(s) 120, 249, 384, 440, 540, 636
HpyF10VI GCNNNNNNNGC 3 cut(s) 327, 437, 579
HpyF3I CTNAG 4 cut(s) 35, 151, 454, 668
HpySE526I ACGT 5 cut(s) 102, 196, 291, 713, 750
Hsp92II CATG 4 cut(s) 284, 426, 484, 654
HspAI GCGC 2 cut(s) 155, 755
Kpn2I TCCGGA 1 cut(s) 362
Kzo9I GATC 4 cut(s) 207, 414, 567, 679
LmnI GCTCC 1 cut(s) 327
Lsp1109I GCAGC 2 cut(s) 633, 769
LweI GCATC 3 cut(s) 177, 258, 330
MaeII ACGT 5 cut(s) 102, 196, 291, 713, 750
MaeIII GTNAC 4 cut(s) 49, 283, 292, 671
MalI GATC 4 cut(s) 209, 416, 569, 681
MboI GATC 4 cut(s) 207, 414, 567, 679
MboII GAAGA 3 cut(s) 362, 597, 771
MhlI GDGCHC 1 cut(s) 542
MluCI AATT 6 cut(s) 43, 55, 63, 94, 183, 387
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 6 cut(s) 30, 341, 405, 463, 635, 771
MroI TCCGGA 1 cut(s) 362
MseI TTAA 4 cut(s) 8, 492, 564, 744
MspI CCGG 2 cut(s) 363, 693
MspR9I CCNGG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 131
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 3 cut(s) 327, 437, 579
NdeII GATC 4 cut(s) 207, 414, 567, 679
NlaIII CATG 4 cut(s) 284, 426, 484, 654
NlaIV GGNNCC 3 cut(s) 268, 329, 552
NmuCI GTSAC 1 cut(s) 49
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 131
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 2 cut(s) 648, 759
Ppu21I YACGTR 1 cut(s) 103
Psp1406I AACGTT 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 3 cut(s) 268, 329, 552
PspPI GGNCC 1 cut(s) 302
PstNI CAGNNNCTG 1 cut(s) 34
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SaqAI TTAA 4 cut(s) 8, 492, 564, 744
SatI GCNGC 2 cut(s) 647, 758
Sau3AI GATC 4 cut(s) 207, 414, 567, 679
Sau96I GGNCC 1 cut(s) 302
ScrFI CCNGG 1 cut(s) 306
SduI GDGCHC 1 cut(s) 542
SfaNI GCATC 3 cut(s) 177, 258, 330
SinI GGWCC 1 cut(s) 302
Sse9I AATT 6 cut(s) 43, 55, 63, 94, 183, 387
SspI AATATT 1 cut(s) 369
StyD4I CCNGG 1 cut(s) 304
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 5 cut(s) 105, 199, 294, 716, 753
TaqI TCGA 1 cut(s) 67
TasI AATT 6 cut(s) 43, 55, 63, 94, 183, 387
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 4 cut(s) 8, 492, 564, 744
Tru9I TTAA 4 cut(s) 8, 492, 564, 744
TscAI CASTG 2 cut(s) 542, 643
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 2 cut(s) 646, 757
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 3 cut(s) 176, 363, 591
TspRI CASTG 2 cut(s) 542, 643
VneI GTGCAC 1 cut(s) 538
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 2 cut(s) 94, 183
XcmI CCANNNNNNNNNTGG 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.