RLG00000020954

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000434
Physical Location & Seq
Forward (+)
70459446 .. 70461955
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020954

Sequence Viewer

Length: 1530 bp
ATGACTCTCAAACTTGTCATGGTTACGTTTTCATTACTTTTGTTCTTATCCTTGACATCCTCGACATCTGGAAGGAGAGAGCTTCTAGCCGATGGTGGTGACTTTGACGTGACCAATGCGAAATATGGTGCAAAGCCCAATTCTGATATTACTGAGGCTTTAGCAAATGCTTGGAAAGATGCCTGTGCATCAACATCGGCAAGTAAAGTTATTGTTCCAAAAGGGACATTCAAAGTAAAAGAAGCAAGTTTCAAAGGTCCTTGCAAGGCTCCTATTGAGGTTCAGGTCCAAGGCACATTACAGGCGCCAGAAGACGCAAGCCAATTATCGAAACAAGATACTTGGATCGAGTTTTTATACCTTAACGACTTCACGCTATCGGGTGGTGGAACCTTTGATGGCCAAGGACAAAAAACTTGGAAAGCCGTTGATCCAAAAAACGCAAAGGCTGGCACCATTAATATCAGGTTCCACGCCGTCAAGAAGTCATTAGTCAAGGATATAACTTCACTTAATAGCAAGAACTTCCACATCAACGTAATAAGTTGCGAGCAACTTACGTTCGATCATGTCACCGTCACTGCACCTGGAGATAGCCCTAACACAGATGGAATACATATCGCGCGCTCAACCGGGGTCAACGTTACTGATACAAATATCGGAACCGGAGATGATTGCATTTCAATTGGTGATGGTACCAAGCAACTTACAATAACTAAGGTTACTTGTGGACCAGGCCATGGAATAAGCGTTGGAAGTCTTGGAAAGTACGACAAAGAAGACCCTGTGGAAGGAATCAATGTTAAGAACTGTACCCTCAGTAAAACGACTAATGGTGTTAGAATCAAGACATGGGCTAAAAATCCTCAAGCTAGCACTTGCTCAGAAATTCACTTTGAGGATATCATCATGAATGATGTTCAAAACCCAATCGTCGTAGATCAAGAATACTGCCCATGGAATCAATGTGATAAACAGGCTTCATCGAAAATCAAGATCAGCAATGTGAGTTTCAAGAACATTAGAGGGACGACTTCCACTCCGTTGGGCGTGAAGATTGTATGCGCCAAGGGCTTACCATGTGAGAAGGTGGAGATGACTGGCATTGATCTCAAATTCACCGGCACAGGTGCACTCACGTCTCAATGTACGAATGTCAAACCCACAATTGCTAATGTGGCACAGCCTCTTGCTTGTGCTACCGATGCTCCTGTACCGGCTGGACCAGCTGAGAAATCCAAGGGCTTCTATGCCGCGAGAGGCGCCGAAGGCAAACTGAAGAAATCACCGTCCCGTAAGCCGCCGACGACTCCTTACTCCTCCATGACACCACCGGCTTTAGCTCCTCCTCGGCTTCAGCCCCTTCGTTGTCGCCATTTGGCACCATGTACTCTACCAGAATTTGGATCTCCTGCTCGCCGCCTGTGGGTTTCAAAAGTTGGGTCAAACAAGGAGGTTGAAGCTGGAGTGCCGAAGGTTGAAGATGGAGAAGAGGACGACTGGGCTCCGAGGAAAGAAGATGACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

510

Amino Acids

54.52

Weight (kDa)

8.48

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 62 - 388 2.9e-101 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 695
AccB1I GGYRCC 5 cut(s) 304, 452, 695, 1262, 1381
AccB7I CCANNNNNTGG 2 cut(s) 740, 1403
AccII CGCG 3 cut(s) 623, 625, 1256
AciI CCGC 3 cut(s) 1254, 1301, 1420
AclI AACGTT 1 cut(s) 642
AclWI GGATC 3 cut(s) 353, 425, 1414
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 3 cut(s) 888, 1115, 1400
AcuI CTGAAG 2 cut(s) 1298, 1340
AcyI GRCGYC 2 cut(s) 305, 1263
AfaI GTAC 6 cut(s) 697, 770, 814, 1150, 1215, 1390
AfiI CCNNNNNNNGG 4 cut(s) 740, 791, 1403, 1426
AgsI TTSAA 8 cut(s) 232, 253, 684, 923, 1015, 1434, 1460, 1481
AjiI CACGTC 2 cut(s) 109, 1140
AjnI CCWGG 2 cut(s) 586, 733
AluBI AGCT 5 cut(s) 82, 872, 1229, 1343, 1463
AluI AGCT 5 cut(s) 82, 872, 1229, 1343, 1463
Alw21I GWGCWC 1 cut(s) 1135
Alw26I GTCTC 1 cut(s) 1146
Alw44I GTGCAC 1 cut(s) 1131
AlwI GGATC 3 cut(s) 353, 425, 1414
AoxI GGCC 2 cut(s) 400, 736
ApaLI GTGCAC 1 cut(s) 1131
ApoI RAATTY 3 cut(s) 888, 1115, 1400
ArsI GACNNNNNNTTYG 2 cut(s) 1427, 1459
AseI ATTAAT 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 695
AspLEI GCGC 5 cut(s) 307, 625, 627, 1067, 1265
AspS9I GGNCC 4 cut(s) 257, 286, 731, 1223
AsuC2I CCSGG 1 cut(s) 634
AsuHPI GGTGA 5 cut(s) 110, 565, 701, 1111, 1278
AsuNHI GCTAGC 1 cut(s) 872
AvaII GGWCC 4 cut(s) 257, 286, 731, 1223
BaeGI GKGCMC 1 cut(s) 1135
BalI TGGCCA 1 cut(s) 402
BanI GGYRCC 5 cut(s) 304, 452, 695, 1262, 1381
BanII GRGCYC 1 cut(s) 1507
BbsI GAAGAC 2 cut(s) 318, 786
Bbv12I GWGCWC 1 cut(s) 1135
BccI CCATC 5 cut(s) 86, 392, 602, 686, 1478
BceAI ACGGC 2 cut(s) 410, 461
BcgI CGANNNNNNTGC 2 cut(s) 177, 211
BciT130I CCWGG 2 cut(s) 588, 735
BcnI CCSGG 1 cut(s) 634
BcoDI GTCTC 1 cut(s) 1146
BfaI CTAG 2 cut(s) 86, 873
BfoI RGCGCY 2 cut(s) 308, 1266
BisI GCNGC 3 cut(s) 1254, 1301, 1420
BlsI GCNGC 3 cut(s) 1255, 1302, 1421
Bme1390I CCNGG 3 cut(s) 588, 634, 735
Bme18I GGWCC 4 cut(s) 257, 286, 731, 1223
BmgBI CACGTC 2 cut(s) 109, 1140
BmgT120I GGNCC 4 cut(s) 257, 286, 731, 1223
BmrFI CCNGG 3 cut(s) 588, 634, 735
BmrI ACTGGG 1 cut(s) 1510
BmsI GCATC 3 cut(s) 169, 197, 1195
BmtI GCTAGC 1 cut(s) 876
BmuI ACTGGG 1 cut(s) 1510
BpiI GAAGAC 2 cut(s) 318, 786
BpmI CTGGAG 2 cut(s) 609, 1485
BpuEI CTTGAG 1 cut(s) 852
BpuMI CCSGG 1 cut(s) 634
BsaHI GRCGYC 2 cut(s) 305, 1263
BsaJI CCNNGG 9 cut(s) 289, 403, 633, 739, 956, 1068, 1239, 1349, 1508
BsaWI WCCGGW 1 cut(s) 665
BsaXI ACNNNNNCTCC 4 cut(s) 1024, 1054, 1192, 1222
Bsc4I CCNNNNNNNGG 4 cut(s) 740, 791, 1403, 1426
Bse118I RCCGGY 3 cut(s) 1121, 1216, 1333
Bse1I ACTGG 2 cut(s) 1105, 1505
Bse3DI GCAATG 1 cut(s) 1009
BseBI CCWGG 2 cut(s) 588, 735
BseDI CCNNGG 9 cut(s) 289, 403, 633, 739, 956, 1068, 1239, 1349, 1508
BseGI GGATG 1 cut(s) 56
BseLI CCNNNNNNNGG 4 cut(s) 740, 791, 1403, 1426
BseMI GCAATG 1 cut(s) 1009
BseMII CTCAG 4 cut(s) 144, 832, 897, 1221
BseNI ACTGG 2 cut(s) 1105, 1505
BsePI GCGCGC 1 cut(s) 623
BseRI GAGGAG 3 cut(s) 1309, 1335, 1338
BseSI GKGCMC 1 cut(s) 1135
BsgI GTGCAG 1 cut(s) 567
Bsh1236I CGCG 3 cut(s) 623, 625, 1256
BshFI GGCC 2 cut(s) 402, 738
BshNI GGYRCC 5 cut(s) 304, 452, 695, 1262, 1381
BsiHKAI GWGCWC 1 cut(s) 1135
BsiSI CCGG 5 cut(s) 633, 666, 1122, 1217, 1334
BslFI GGGAC 3 cut(s) 238, 1042, 1276
BslI CCNNNNNNNGG 4 cut(s) 740, 791, 1403, 1426
BsmAI GTCTC 1 cut(s) 1146
BsmBI CGTCTC 1 cut(s) 1146
BsmFI GGGAC 3 cut(s) 238, 1042, 1276
BsnI GGCC 2 cut(s) 402, 738
Bsp1286I GDGCHC 2 cut(s) 1135, 1507
Bsp143I GATC 7 cut(s) 345, 430, 565, 940, 996, 1108, 1406
Bsp19I CCATGG 2 cut(s) 739, 956
BspACI CCGC 3 cut(s) 1254, 1301, 1420
BspANI GGCC 2 cut(s) 402, 738
BspCNI CTCAG 4 cut(s) 145, 831, 896, 1222
BspFNI CGCG 3 cut(s) 623, 625, 1256
BspHI TCATGA 1 cut(s) 909
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 3 cut(s) 353, 425, 1414
BspT107I GGYRCC 5 cut(s) 304, 452, 695, 1262, 1381
BsrDI GCAATG 1 cut(s) 1009
BsrFI RCCGGY 3 cut(s) 1121, 1216, 1333
BsrI ACTGG 2 cut(s) 1105, 1505
BssAI RCCGGY 3 cut(s) 1121, 1216, 1333
BssECI CCNNGG 9 cut(s) 289, 403, 633, 739, 956, 1068, 1239, 1349, 1508
BssHII GCGCGC 1 cut(s) 623
BssMI GATC 7 cut(s) 345, 430, 565, 940, 996, 1108, 1406
BssNI GRCGYC 2 cut(s) 305, 1263
BssT1I CCWWGG 6 cut(s) 289, 403, 739, 956, 1068, 1239
Bst2UI CCWGG 2 cut(s) 588, 735
Bst4CI ACNGT 3 cut(s) 577, 812, 1290
Bst6I CTCTTC 1 cut(s) 1485
BstACI GRCGYC 2 cut(s) 305, 1263
BstC8I GCNNGC 6 cut(s) 319, 451, 551, 625, 874, 1417
BstDEI CTNAG 5 cut(s) 153, 717, 818, 883, 1230
BstDSI CCRYGG 2 cut(s) 739, 956
BstENI CCTNNNNNAGG 1 cut(s) 789
BstF5I GGATG 1 cut(s) 56
BstFNI CGCG 3 cut(s) 623, 625, 1256
BstH2I RGCGCY 2 cut(s) 308, 1266
BstHHI GCGC 5 cut(s) 307, 625, 627, 1067, 1265
BstKTI GATC 7 cut(s) 348, 433, 568, 943, 999, 1111, 1409
BstMAI GTCTC 1 cut(s) 1146
BstMBI GATC 7 cut(s) 345, 430, 565, 940, 996, 1108, 1406
BstMWI GCNNNNNNNGC 6 cut(s) 1071, 1178, 1205, 1226, 1262, 1269
BstNI CCWGG 2 cut(s) 588, 735
BstSCI CCNGG 3 cut(s) 586, 632, 733
BstSLI GKGCMC 1 cut(s) 1135
BstUI CGCG 3 cut(s) 623, 625, 1256
BstV2I GAAGAC 2 cut(s) 318, 786
BstX2I RGATCY 1 cut(s) 1406
BstXI CCANNNNNNTGG 1 cut(s) 1045
BstYI RGATCY 1 cut(s) 1406
BsuRI GGCC 2 cut(s) 402, 738
BtgI CCRYGG 2 cut(s) 739, 956
BtrI CACGTC 2 cut(s) 109, 1140
BtsCI GGATG 1 cut(s) 56
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 6 cut(s) 319, 451, 551, 625, 874, 1417
CciI TCATGA 1 cut(s) 909
CfoI GCGC 5 cut(s) 307, 625, 627, 1067, 1265
Cfr10I RCCGGY 3 cut(s) 1121, 1216, 1333
Cfr13I GGNCC 4 cut(s) 257, 286, 731, 1223
CseI GACGC 1 cut(s) 323
Csp6I GTAC 6 cut(s) 696, 769, 813, 1149, 1214, 1389
CviAII CATG 9 cut(s) 19, 569, 740, 852, 910, 957, 1080, 1324, 1386
CviQI GTAC 6 cut(s) 696, 769, 813, 1149, 1214, 1389
DdeI CTNAG 5 cut(s) 153, 717, 818, 883, 1230
DinI GGCGCC 2 cut(s) 306, 1264
DpnI GATC 7 cut(s) 347, 432, 567, 942, 998, 1110, 1408
DpnII GATC 7 cut(s) 345, 430, 565, 940, 996, 1108, 1406
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 1485
EarI CTCTTC 1 cut(s) 1485
Eco130I CCWWGG 6 cut(s) 289, 403, 739, 956, 1068, 1239
Eco24I GRGCYC 1 cut(s) 1507
Eco32I GATATC 1 cut(s) 904
Eco47I GGWCC 4 cut(s) 257, 286, 731, 1223
Eco57I CTGAAG 2 cut(s) 1298, 1340
EcoNI CCTNNNNNAGG 1 cut(s) 789
EcoO109I RGGNCCY 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 586, 733
EcoRV GATATC 1 cut(s) 904
EcoT14I CCWWGG 6 cut(s) 289, 403, 739, 956, 1068, 1239
EcoT38I GRGCYC 1 cut(s) 1507
EgeI GGCGCC 2 cut(s) 306, 1264
EheI GGCGCC 2 cut(s) 306, 1264
ErhI CCWWGG 6 cut(s) 289, 403, 739, 956, 1068, 1239
Esp3I CGTCTC 1 cut(s) 1146
FaeI CATG 9 cut(s) 22, 572, 743, 855, 913, 960, 1083, 1327, 1389
FaqI GGGAC 3 cut(s) 238, 1042, 1276
FatI CATG 9 cut(s) 18, 568, 739, 851, 909, 956, 1079, 1323, 1385
Fnu4HI GCNGC 3 cut(s) 1254, 1301, 1420
FokI GGATG 1 cut(s) 43
FriOI GRGCYC 1 cut(s) 1507
Fsp4HI GCNGC 3 cut(s) 1254, 1301, 1420
FspBI CTAG 2 cut(s) 86, 873
GlaI GCGC 5 cut(s) 306, 624, 626, 1066, 1264
GluI GCNGC 3 cut(s) 1254, 1301, 1420
GsuI CTGGAG 2 cut(s) 609, 1485
HaeII RGCGCY 2 cut(s) 308, 1266
HaeIII GGCC 2 cut(s) 402, 738
HapII CCGG 5 cut(s) 633, 666, 1122, 1217, 1334
HgaI GACGC 1 cut(s) 323
HhaI GCGC 5 cut(s) 307, 625, 627, 1067, 1265
Hin1I GRCGYC 2 cut(s) 305, 1263
Hin1II CATG 9 cut(s) 22, 572, 743, 855, 913, 960, 1083, 1327, 1389
Hin6I GCGC 5 cut(s) 305, 623, 625, 1065, 1263
HinP1I GCGC 5 cut(s) 305, 623, 625, 1065, 1263
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HinfI GANTC 5 cut(s) 4, 795, 843, 961, 1309
HpaII CCGG 5 cut(s) 633, 666, 1122, 1217, 1334
HphI GGTGA 5 cut(s) 110, 565, 701, 1111, 1278
Hpy166II GTNNAC 3 cut(s) 640, 731, 1133
Hpy188I TCNGA 4 cut(s) 145, 662, 886, 1509
Hpy188III TCNNGA 7 cut(s) 69, 481, 847, 910, 944, 994, 1015
Hpy8I GTNNAC 3 cut(s) 640, 731, 1133
Hpy99I CGWCG 2 cut(s) 938, 1309
HpyAV CCTTC 6 cut(s) 66, 785, 1081, 1262, 1373, 1468
HpyCH4III ACNGT 3 cut(s) 577, 812, 1290
HpyCH4IV ACGT 6 cut(s) 26, 108, 537, 560, 642, 1139
HpyCH4V TGCA 6 cut(s) 131, 188, 264, 584, 678, 1133
HpyF10VI GCNNNNNNNGC 6 cut(s) 1071, 1178, 1205, 1226, 1262, 1269
HpyF3I CTNAG 5 cut(s) 153, 717, 818, 883, 1230
HpySE526I ACGT 6 cut(s) 26, 108, 537, 560, 642, 1139
Hsp92I GRCGYC 2 cut(s) 305, 1263
Hsp92II CATG 9 cut(s) 22, 572, 743, 855, 913, 960, 1083, 1327, 1389
HspAI GCGC 5 cut(s) 305, 623, 625, 1065, 1263
KasI GGCGCC 2 cut(s) 304, 1262
KpnI GGTACC 1 cut(s) 699
Kzo9I GATC 7 cut(s) 345, 430, 565, 940, 996, 1108, 1406
LmnI GCTCC 4 cut(s) 274, 1213, 1348, 1510
LweI GCATC 3 cut(s) 169, 197, 1195
MaeI CTAG 2 cut(s) 86, 873
MaeII ACGT 6 cut(s) 26, 108, 537, 560, 642, 1139
MaeIII GTNAC 7 cut(s) 22, 98, 109, 571, 577, 643, 721
MalI GATC 7 cut(s) 347, 432, 567, 942, 998, 1110, 1408
MboI GATC 7 cut(s) 345, 430, 565, 940, 996, 1108, 1406
MboII GAAGA 7 cut(s) 323, 791, 1066, 1291, 1493, 1502, 1529
MfeI CAATTG 2 cut(s) 684, 1167
MflI RGATCY 1 cut(s) 1406
MhlI GDGCHC 2 cut(s) 1135, 1507
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 7 cut(s) 139, 323, 684, 888, 1115, 1167, 1400
MluNI TGGCCA 1 cut(s) 402
Mly113I GGCGCC 2 cut(s) 305, 1263
MlyI GAGTC 1 cut(s) 1303
MmeI TCCRAC 1 cut(s) 733
Mox20I TGGCCA 1 cut(s) 402
MscI TGGCCA 1 cut(s) 402
MseI TTAA 4 cut(s) 363, 459, 513, 804
Msp20I TGGCCA 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 1229
MspI CCGG 5 cut(s) 633, 666, 1122, 1217, 1334
MspR9I CCNGG 3 cut(s) 588, 634, 735
MunI CAATTG 2 cut(s) 684, 1167
MvaI CCWGG 2 cut(s) 588, 735
MvnI CGCG 3 cut(s) 623, 625, 1256
MwoI GCNNNNNNNGC 6 cut(s) 1071, 1178, 1205, 1226, 1262, 1269
NarI GGCGCC 2 cut(s) 305, 1263
NciI CCSGG 1 cut(s) 634
NcoI CCATGG 2 cut(s) 739, 956
NdeII GATC 7 cut(s) 345, 430, 565, 940, 996, 1108, 1406
NheI GCTAGC 1 cut(s) 872
NlaIII CATG 9 cut(s) 22, 572, 743, 855, 913, 960, 1083, 1327, 1389
NmeAIII GCCGAG 1 cut(s) 1330
NmuCI GTSAC 4 cut(s) 98, 109, 571, 577
PagI TCATGA 1 cut(s) 909
PauI GCGCGC 1 cut(s) 623
PfeI GAWTC 3 cut(s) 795, 843, 961
PflMI CCANNNNNTGG 2 cut(s) 740, 1403
PkrI GCNGC 3 cut(s) 1255, 1302, 1421
PleI GAGTC 1 cut(s) 1303
PluTI GGCGCC 2 cut(s) 308, 1266
PpsI GAGTC 1 cut(s) 1303
PpuMI RGGWCCY 1 cut(s) 257
PshBI ATTAAT 1 cut(s) 459
Psp1406I AACGTT 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 586, 733
PspGI CCWGG 2 cut(s) 586, 733
PspPI GGNCC 4 cut(s) 257, 286, 731, 1223
PspPPI RGGWCCY 1 cut(s) 257
PsuI RGATCY 1 cut(s) 1406
PteI GCGCGC 1 cut(s) 623
PvuII CAGCTG 1 cut(s) 1229
RsaI GTAC 6 cut(s) 697, 770, 814, 1150, 1215, 1390
RsaNI GTAC 6 cut(s) 696, 769, 813, 1149, 1214, 1389
SaqAI TTAA 4 cut(s) 363, 459, 513, 804
SatI GCNGC 3 cut(s) 1254, 1301, 1420
Sau3AI GATC 7 cut(s) 345, 430, 565, 940, 996, 1108, 1406
Sau96I GGNCC 4 cut(s) 257, 286, 731, 1223
SchI GAGTC 1 cut(s) 1303
ScrFI CCNGG 3 cut(s) 588, 634, 735
SduI GDGCHC 2 cut(s) 1135, 1507
SfaNI GCATC 3 cut(s) 169, 197, 1195
SfoI GGCGCC 2 cut(s) 306, 1264
SinI GGWCC 4 cut(s) 257, 286, 731, 1223
SmlI CTYRAG 1 cut(s) 867
SmoI CTYRAG 1 cut(s) 867
Sse9I AATT 7 cut(s) 139, 323, 684, 888, 1115, 1167, 1400
SsiI CCGC 3 cut(s) 1254, 1301, 1420
SspDI GGCGCC 2 cut(s) 304, 1262
SspMI CTAG 2 cut(s) 86, 873
StyD4I CCNGG 3 cut(s) 586, 632, 733
StyI CCWWGG 6 cut(s) 289, 403, 739, 956, 1068, 1239
TaaI ACNGT 3 cut(s) 577, 812, 1290
TaiI ACGT 6 cut(s) 29, 111, 540, 563, 645, 1142
TaqI TCGA 5 cut(s) 62, 329, 348, 564, 986
TasI AATT 7 cut(s) 139, 323, 684, 888, 1115, 1167, 1400
TatI WGTACW 1 cut(s) 1388
TauI GCSGC 3 cut(s) 1256, 1303, 1422
TfiI GAWTC 3 cut(s) 795, 843, 961
Tru1I TTAA 4 cut(s) 363, 459, 513, 804
Tru9I TTAA 4 cut(s) 363, 459, 513, 804
TscAI CASTG 1 cut(s) 586
TseFI GTSAC 4 cut(s) 98, 109, 571, 577
Tsp45I GTSAC 4 cut(s) 98, 109, 571, 577
TspDTI ATGAA 3 cut(s) 21, 926, 972
TspGWI ACGGA 1 cut(s) 1032
TspRI CASTG 1 cut(s) 586
Van91I CCANNNNNTGG 2 cut(s) 740, 1403
VneI GTGCAC 1 cut(s) 1131
VpaK11BI GGWCC 4 cut(s) 257, 286, 731, 1223
VspI ATTAAT 1 cut(s) 459
XagI CCTNNNNNAGG 1 cut(s) 789
XapI RAATTY 3 cut(s) 888, 1115, 1400
XspI CTAG 2 cut(s) 86, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.