RchiOBHm_Chr2g0159061

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000434
Physical Location & Seq
Forward (+)
75137314 .. 75138419
1106 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52772

Sequence Viewer

Length: 975 bp
ATGGGTCTCAAATTGATGACTATCATGGCGATATTCATGTTCTTATTGTTAGCAGCTACAGCTAAAGCCCAACCCTGTGTAGTCTTTGAAGTGACGAGTGCAAAATATGGTGGAAAAACTAACTATGATATTACAGACTCCTTAGCACAAGCTTGGACAGATGCTTGTGTTTCTGTATGGTCGAGTAAACTCGTTGTTCCAAGCGGCACATATTGGTTAAGAGGAGCAACTTTTATAGGTCCTTGCAAGGCTCCTATCAAGGTGCAAATCCAAGGAAAGTTGAAAGATCCCGAAGATGGCCACCAACTCGTGAACTCGGATTTTTGGGTTGCTTTTCGGTACATTGACAGGCTCTACTTATCAGGTGGTGGAACTTTTGATGGCCAAGGAGCCCTTGGGTGTAACTATCGTAGCGAGAGTTCTAATACAGATGGAATCCACATTGCATATTCGTGGAGGATCAACGTAACGGATGCAAACATCGAAACTAGAGATTATTGTATTTCTATCGGTAATGGCACTTCGCAACTCACAGTAACCAATGTTAATTGTGGACCGGGACATGGAATAAGCATAGGAAGTCTTGGAAAGTATCTTGATGAAAAACCGGTATATGGCCTCATCATTAAGAACTGCACCCTCACGAACACAACAAACGGTGTGAGAATCAAGACATGGCCTAGTTCTCCCAGTGCTCGTTTTGCCTCACACATATACTTTGAGGATATTATCATGAATAATGTGAAGAACCCCATCATTATAGACCAAAACTACTGCCCCAGGCATCGTTGCAAACAACAGGCTCAGTTGAACACTAAGATTAGTAATGTCAGCTTCAAGAACATTAGAGGCTCATCTGCATCACAAGTTGCCATCAATATTGCATGTAGTGGGAGCTTACCATGTGAGAATGTAGAATTGATAGGCATTGATCTAGAATACAGTGGAGATGGCGATCGACCTATTACATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

324

Amino Acids

35.35

Weight (kDa)

8.23

Isoelectric Point (pI)

26.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 131 7.1e-08 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 136 - 317 1.7e-57 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 204
AclWI GGATC 2 cut(s) 281, 467
AcoI YGGCCR 2 cut(s) 298, 382
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 3 cut(s) 296, 563, 614
AgeI ACCGGT 1 cut(s) 607
AgsI TTSAA 4 cut(s) 89, 283, 811, 838
AjnI CCWGG 1 cut(s) 779
AluBI AGCT 5 cut(s) 56, 62, 152, 834, 897
AluI AGCT 5 cut(s) 56, 62, 152, 834, 897
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 2 cut(s) 281, 467
AoxI GGCC 4 cut(s) 298, 382, 616, 677
ApeKI GCWGC 1 cut(s) 53
AsiGI ACCGGT 1 cut(s) 607
AspS9I GGNCC 2 cut(s) 239, 554
AsuC2I CCSGG 1 cut(s) 558
AvaII GGWCC 2 cut(s) 239, 554
BalI TGGCCA 2 cut(s) 300, 384
BanII GRGCYC 1 cut(s) 394
BarI GAAGNNNNNNTAC 2 cut(s) 505, 537
BauI CACGAG 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 1 cut(s) 65
BccI CCATC 6 cut(s) 290, 374, 425, 761, 881, 944
BciT130I CCWGG 1 cut(s) 781
BcnI CCSGG 1 cut(s) 558
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 3 cut(s) 489, 681, 935
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 2 cut(s) 54, 205
BlsI GCNGC 2 cut(s) 55, 206
Bme1390I CCNGG 2 cut(s) 558, 781
Bme18I GGWCC 2 cut(s) 239, 554
BmgT120I GGNCC 2 cut(s) 239, 554
BmiI GGNNCC 2 cut(s) 252, 391
BmrFI CCNGG 2 cut(s) 558, 781
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 4 cut(s) 151, 463, 793, 869
BmuI ACTGGG 1 cut(s) 684
Bpu10I CCTNAGC 1 cut(s) 142
BpuMI CCSGG 1 cut(s) 558
BsaBI GATNNNNATC 2 cut(s) 20, 954
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 271, 385, 394, 779
BsaWI WCCGGW 1 cut(s) 607
Bsc4I CCNNNNNNNGG 3 cut(s) 296, 563, 614
Bse118I RCCGGY 1 cut(s) 607
Bse1I ACTGG 1 cut(s) 690
Bse3DI GCAATG 1 cut(s) 441
Bse8I GATNNNNATC 2 cut(s) 20, 954
BseBI CCWGG 1 cut(s) 781
BseDI CCNNGG 4 cut(s) 271, 385, 394, 779
BseGI GGATG 1 cut(s) 478
BseJI GATNNNNATC 2 cut(s) 20, 954
BseLI CCNNNNNNNGG 3 cut(s) 296, 563, 614
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 1 cut(s) 818
BseNI ACTGG 1 cut(s) 690
BseRI GAGGAG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 65
BsgI GTGCAG 1 cut(s) 619
Bsh1285I CGRYCG 1 cut(s) 958
BshFI GGCC 4 cut(s) 300, 384, 618, 679
BshTI ACCGGT 1 cut(s) 607
BsiEI CGRYCG 1 cut(s) 958
BsiHKAI GWGCWC 1 cut(s) 697
BsiSI CCGG 2 cut(s) 557, 608
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 3 cut(s) 296, 563, 614
BsmAI GTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 573
BsnI GGCC 4 cut(s) 300, 384, 618, 679
Bso31I GGTCTC 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 394, 697
Bsp143I GATC 4 cut(s) 286, 459, 931, 955
BspACI CCGC 1 cut(s) 204
BspANI GGCC 4 cut(s) 300, 384, 618, 679
BspCNI CTCAG 1 cut(s) 817
BspHI TCATGA 1 cut(s) 732
BspLI GGNNCC 2 cut(s) 252, 391
BspPI GGATC 2 cut(s) 281, 467
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 607
BsrI ACTGG 1 cut(s) 690
BssAI RCCGGY 1 cut(s) 607
BssECI CCNNGG 4 cut(s) 271, 385, 394, 779
BssMI GATC 4 cut(s) 286, 459, 931, 955
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 3 cut(s) 271, 385, 394
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 781
Bst4CI ACNGT 3 cut(s) 535, 659, 944
BstDEI CTNAG 3 cut(s) 142, 804, 816
BstF5I GGATG 1 cut(s) 478
BstKTI GATC 4 cut(s) 289, 462, 934, 958
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 4 cut(s) 286, 459, 931, 955
BstMCI CGRYCG 1 cut(s) 958
BstMWI GCNNNNNNNGC 2 cut(s) 59, 701
BstNI CCWGG 1 cut(s) 781
BstNSI RCATGY 1 cut(s) 888
BstSCI CCNGG 2 cut(s) 556, 779
BstSFI CTRYAG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 65
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BsuRI GGCC 4 cut(s) 300, 384, 618, 679
BtsCI GGATG 1 cut(s) 478
BtsIMutI CAGTG 2 cut(s) 697, 949
CciI TCATGA 1 cut(s) 732
Cfr10I RCCGGY 1 cut(s) 607
Cfr13I GGNCC 2 cut(s) 239, 554
Csp6I GTAC 1 cut(s) 340
CspAI ACCGGT 1 cut(s) 607
CviAII CATG 7 cut(s) 25, 37, 563, 675, 733, 885, 903
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 3 cut(s) 142, 804, 816
DpnI GATC 4 cut(s) 288, 461, 933, 957
DpnII GATC 4 cut(s) 286, 459, 931, 955
EaeI YGGCCR 2 cut(s) 298, 382
Eco130I CCWWGG 3 cut(s) 271, 385, 394
Eco24I GRGCYC 1 cut(s) 394
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 2 cut(s) 239, 554
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 779
EcoT14I CCWWGG 3 cut(s) 271, 385, 394
EcoT38I GRGCYC 1 cut(s) 394
ErhI CCWWGG 3 cut(s) 271, 385, 394
FaeI CATG 7 cut(s) 28, 40, 566, 678, 736, 888, 906
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 1 cut(s) 573
FatI CATG 7 cut(s) 24, 36, 562, 674, 732, 884, 902
Fnu4HI GCNGC 2 cut(s) 54, 205
FokI GGATG 1 cut(s) 485
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 54, 205
FspBI CTAG 3 cut(s) 489, 681, 935
GluI GCNGC 2 cut(s) 54, 205
HaeIII GGCC 4 cut(s) 300, 384, 618, 679
HapII CCGG 2 cut(s) 557, 608
Hin1II CATG 7 cut(s) 28, 40, 566, 678, 736, 888, 906
HindIII AAGCTT 1 cut(s) 150
HinfI GANTC 3 cut(s) 137, 435, 666
HpaII CCGG 2 cut(s) 557, 608
Hpy166II GTNNAC 3 cut(s) 188, 313, 554
Hpy188I TCNGA 1 cut(s) 319
Hpy188III TCNNGA 9 cut(s) 290, 310, 596, 643, 670, 733, 838, 935, 972
Hpy8I GTNNAC 3 cut(s) 188, 313, 554
HpyCH4III ACNGT 3 cut(s) 535, 659, 944
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 9 cut(s) 101, 246, 265, 446, 476, 636, 792, 860, 884
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 701
HpyF3I CTNAG 3 cut(s) 142, 804, 816
HpySE526I ACGT 1 cut(s) 465
Hsp92II CATG 7 cut(s) 28, 40, 566, 678, 736, 888, 906
Kzo9I GATC 4 cut(s) 286, 459, 931, 955
LmnI GCTCC 4 cut(s) 224, 256, 389, 894
LpnPI CCDG 9 cut(s) 88, 334, 348, 570, 621, 703, 766, 785, 793
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 4 cut(s) 151, 463, 793, 869
MaeI CTAG 3 cut(s) 489, 681, 935
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 4 cut(s) 91, 401, 466, 535
MalI GATC 4 cut(s) 288, 461, 933, 957
MboI GATC 4 cut(s) 286, 459, 931, 955
MboII GAAGA 2 cut(s) 305, 757
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 2 cut(s) 394, 697
MlsI TGGCCA 2 cut(s) 300, 384
MluCI AATT 3 cut(s) 11, 547, 917
MluNI TGGCCA 2 cut(s) 300, 384
MlyI GAGTC 1 cut(s) 131
MnlI CCTC 7 cut(s) 215, 450, 629, 650, 715, 715, 842
Mox20I TGGCCA 2 cut(s) 300, 384
MscI TGGCCA 2 cut(s) 300, 384
MseI TTAA 3 cut(s) 218, 546, 627
MslI CAYNNNNRTG 1 cut(s) 451
Msp20I TGGCCA 2 cut(s) 300, 384
MspI CCGG 2 cut(s) 557, 608
MspR9I CCNGG 2 cut(s) 558, 781
MvaI CCWGG 1 cut(s) 781
MwoI GCNNNNNNNGC 2 cut(s) 59, 701
NciI CCSGG 1 cut(s) 558
NdeII GATC 4 cut(s) 286, 459, 931, 955
NlaIII CATG 7 cut(s) 28, 40, 566, 678, 736, 888, 906
NlaIV GGNNCC 2 cut(s) 252, 391
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 1 cut(s) 888
PagI TCATGA 1 cut(s) 732
PfeI GAWTC 2 cut(s) 435, 666
PinAI ACCGGT 1 cut(s) 607
PkrI GCNGC 2 cut(s) 55, 206
Ple19I CGATCG 1 cut(s) 958
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PpuMI RGGWCCY 1 cut(s) 239
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 779
PspGI CCWGG 1 cut(s) 779
PspN4I GGNNCC 2 cut(s) 252, 391
PspPI GGNCC 2 cut(s) 239, 554
PspPPI RGGWCCY 1 cut(s) 239
PsrI GAACNNNNNNTAC 2 cut(s) 403, 435
PsuI RGATCY 1 cut(s) 286
PvuI CGATCG 1 cut(s) 958
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 1 cut(s) 451
SaqAI TTAA 3 cut(s) 218, 546, 627
SatI GCNGC 2 cut(s) 54, 205
Sau3AI GATC 4 cut(s) 286, 459, 931, 955
Sau96I GGNCC 2 cut(s) 239, 554
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 2 cut(s) 558, 781
SduI GDGCHC 2 cut(s) 394, 697
SfaNI GCATC 4 cut(s) 151, 463, 793, 869
SfcI CTRYAG 1 cut(s) 57
SinI GGWCC 2 cut(s) 239, 554
SmiMI CAYNNNNRTG 1 cut(s) 451
Sse9I AATT 3 cut(s) 11, 547, 917
SsiI CCGC 1 cut(s) 204
SspI AATATT 1 cut(s) 880
SspMI CTAG 3 cut(s) 489, 681, 935
StyD4I CCNGG 2 cut(s) 556, 779
StyI CCWWGG 3 cut(s) 271, 385, 394
TaaI ACNGT 3 cut(s) 535, 659, 944
TaiI ACGT 1 cut(s) 468
TaqI TCGA 3 cut(s) 182, 483, 958
TasI AATT 3 cut(s) 11, 547, 917
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 2 cut(s) 435, 666
Tru1I TTAA 3 cut(s) 218, 546, 627
Tru9I TTAA 3 cut(s) 218, 546, 627
TscAI CASTG 2 cut(s) 697, 949
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 3 cut(s) 25, 615, 749
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 2 cut(s) 697, 949
VpaK11BI GGWCC 2 cut(s) 239, 554
XbaI TCTAGA 1 cut(s) 934
XceI RCATGY 1 cut(s) 888
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XspI CTAG 3 cut(s) 489, 681, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.