MD13G1218300.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000434
Physical Location & Seq
Reverse (-)
20971521 .. 20972315
795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1218300.v1.1.491

Sequence Viewer

Length: 795 bp
ATGTTAACAGAAGCAAGTTTCAAAGGTCCTTGCAAGGCTCCTATTGAGATTCAAGTACAAGGGAAATTAACAGCTCCAGCAGACGGCGGCAAACTTACGAGACCGGATACTTGGGTTGGTTTTGACCACGTCAACAAGCTTACCGTATCAGGTGGTGGAACTTTTGACGGCCAAGGAGCACTTGCTTGGAAGAAAAATGACTGCAACAAAAACAAAGATTGCAAATCTATTGCCATCAATATGAGGTTCAACTTCGTTACCAATTCCACAATTCAGGACATAACTTCCCTCGACAGCAAGAATTTCCACGTCAACGTTTTGGGGTGCCACAATGTCACATTCCAACGTGTCAATGTCACGGCACCGGGGGAGAGCATAAATACAGATGGAATCCATATCGGGCGTTCAACTGGAATCAACATTACTGATGCACACATTGGAACTGGGGATGACTGTGTTTCCATTGGCGATGGAAACAAAGAAATCACCGTAACTAGGGTTACTTGTGGACCAGGCCATGGCATAAGCGTTGGAAGTCTTGGAAGGTATCCGAATGAAGAACCTGTGGTTGGAATCAGAGTTAAGAACTGCACCTTGACTAATACATCCAACGGTGTGAGAATCAAGACATGGCCTGCTTCTCCTTCGGATGGTATTGCATCAGATATGCATTTTGAAGATATTATCATGGTCAATGTCAGCAATCCTGTCCTCATAGACCAACAGTACTGCCCGTGGAACCAGTGTAAACAGAACGTTCCATCAAAAGTTAAGATCAGCAATGTCAGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

265

Amino Acids

28.36

Weight (kDa)

7.57

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 2 - 264 2e-86 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 324, 361
AccB7I CCANNNNNTGG 1 cut(s) 518
AciI CCGC 1 cut(s) 87
AclI AACGTT 2 cut(s) 315, 756
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 1 cut(s) 301
AfaI GTAC 2 cut(s) 57, 728
AfiI CCNNNNNNNGG 5 cut(s) 83, 495, 518, 569, 650
AflIII ACRYGT 1 cut(s) 346
AgsI TTSAA 5 cut(s) 22, 53, 250, 408, 677
AjiI CACGTC 2 cut(s) 130, 310
AjnI CCWGG 1 cut(s) 511
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 3 cut(s) 74, 139, 789
AluI AGCT 3 cut(s) 74, 139, 789
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 1 cut(s) 94
AoxI GGCC 3 cut(s) 169, 514, 632
ApoI RAATTY 1 cut(s) 301
AspS9I GGNCC 2 cut(s) 26, 509
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 1 cut(s) 478
AvaII GGWCC 2 cut(s) 26, 509
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BanI GGYRCC 2 cut(s) 324, 361
Bbv12I GWGCWC 1 cut(s) 181
BccI CCATC 5 cut(s) 242, 380, 464, 644, 769
BceAI ACGGC 3 cut(s) 100, 184, 375
BciT130I CCWGG 1 cut(s) 513
BciVI GTATCC 2 cut(s) 100, 558
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 1 cut(s) 495
BfuI GTATCC 2 cut(s) 100, 558
BisI GCNGC 1 cut(s) 88
BlsI GCNGC 1 cut(s) 89
BmcAI AGTACT 1 cut(s) 728
Bme1390I CCNGG 2 cut(s) 366, 513
Bme18I GGWCC 2 cut(s) 26, 509
BmgBI CACGTC 2 cut(s) 130, 310
BmgT120I GGNCC 2 cut(s) 26, 509
BmiI GGNNCC 4 cut(s) 39, 326, 363, 740
BmrFI CCNGG 2 cut(s) 366, 513
BmrI ACTGGG 1 cut(s) 453
BmsI GCATC 2 cut(s) 418, 668
BmuI ACTGGG 1 cut(s) 453
BpmI CTGGAG 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 4 cut(s) 172, 365, 517, 734
BsaWI WCCGGW 1 cut(s) 103
Bsc4I CCNNNNNNNGG 5 cut(s) 83, 495, 518, 569, 650
Bse1I ACTGG 3 cut(s) 415, 448, 742
Bse3DI GCAATG 1 cut(s) 787
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 4 cut(s) 172, 365, 517, 734
BseGI GGATG 3 cut(s) 454, 605, 655
BseLI CCNNNNNNNGG 5 cut(s) 83, 495, 518, 569, 650
BseMI GCAATG 1 cut(s) 787
BseNI ACTGG 3 cut(s) 415, 448, 742
BsgI GTGCAG 1 cut(s) 574
BshFI GGCC 3 cut(s) 171, 516, 634
BshNI GGYRCC 2 cut(s) 324, 361
BsiHKAI GWGCWC 1 cut(s) 181
BsiSI CCGG 2 cut(s) 104, 365
BslI CCNNNNNNNGG 5 cut(s) 83, 495, 518, 569, 650
BsmAI GTCTC 1 cut(s) 94
BsnI GGCC 3 cut(s) 171, 516, 634
Bso31I GGTCTC 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 1 cut(s) 774
Bsp19I CCATGG 1 cut(s) 517
BspACI CCGC 1 cut(s) 87
BspANI GGCC 3 cut(s) 171, 516, 634
BspLI GGNNCC 4 cut(s) 39, 326, 363, 740
BspT107I GGYRCC 2 cut(s) 324, 361
BspTNI GGTCTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 787
BsrI ACTGG 3 cut(s) 415, 448, 742
BssECI CCNNGG 4 cut(s) 172, 365, 517, 734
BssMI GATC 1 cut(s) 774
BssT1I CCWWGG 2 cut(s) 172, 517
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 5 cut(s) 145, 455, 490, 614, 726
BstC8I GCNNGC 1 cut(s) 636
BstDSI CCRYGG 2 cut(s) 517, 734
BstF5I GGATG 3 cut(s) 454, 605, 655
BstKTI GATC 1 cut(s) 777
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 1 cut(s) 774
BstMWI GCNNNNNNNGC 1 cut(s) 786
BstNI CCWGG 1 cut(s) 513
BstSCI CCNGG 2 cut(s) 364, 511
BsuI GTATCC 2 cut(s) 100, 558
BsuRI GGCC 3 cut(s) 171, 516, 634
BtgI CCRYGG 2 cut(s) 517, 734
BtgZI GCGATG 1 cut(s) 483
BtrI CACGTC 2 cut(s) 130, 310
BtsCI GGATG 3 cut(s) 454, 605, 655
BtsIMutI CAGTG 1 cut(s) 749
Cac8I GCNNGC 1 cut(s) 636
Cfr13I GGNCC 2 cut(s) 26, 509
Csp6I GTAC 2 cut(s) 56, 727
CviAII CATG 3 cut(s) 518, 630, 688
CviJI RGCY 7 cut(s) 38, 74, 139, 171, 516, 634, 789
CviKI_1 RGCY 7 cut(s) 38, 74, 139, 171, 516, 634, 789
CviQI GTAC 2 cut(s) 56, 727
DpnI GATC 1 cut(s) 776
DpnII GATC 1 cut(s) 774
EaeI YGGCCR 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 172, 517
Eco31I GGTCTC 1 cut(s) 94
Eco47I GGWCC 2 cut(s) 26, 509
EcoO109I RGGNCCY 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 2 cut(s) 172, 517
EcoT22I ATGCAT 1 cut(s) 672
ErhI CCWWGG 2 cut(s) 172, 517
FaeI CATG 3 cut(s) 521, 633, 691
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FatI CATG 3 cut(s) 517, 629, 687
Fnu4HI GCNGC 1 cut(s) 88
FokI GGATG 3 cut(s) 461, 592, 662
Fsp4HI GCNGC 1 cut(s) 88
FspBI CTAG 1 cut(s) 495
GluI GCNGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 3 cut(s) 171, 516, 634
HapII CCGG 2 cut(s) 104, 365
Hin1II CATG 3 cut(s) 521, 633, 691
HincII GTYRAC 3 cut(s) 6, 133, 313
HindII GTYRAC 3 cut(s) 6, 133, 313
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 5 cut(s) 49, 390, 414, 573, 621
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 2 cut(s) 104, 365
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 5 cut(s) 6, 133, 313, 509, 749
Hpy188I TCNGA 4 cut(s) 552, 578, 649, 664
Hpy188III TCNNGA 2 cut(s) 275, 625
Hpy8I GTNNAC 5 cut(s) 6, 133, 313, 509, 749
HpyAV CCTTC 2 cut(s) 537, 654
HpyCH4III ACNGT 5 cut(s) 145, 455, 490, 614, 726
HpyCH4IV ACGT 5 cut(s) 129, 309, 315, 346, 756
HpyCH4V TGCA 7 cut(s) 33, 204, 222, 431, 591, 659, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 786
HpySE526I ACGT 5 cut(s) 129, 309, 315, 346, 756
Hsp92II CATG 3 cut(s) 521, 633, 691
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 774
LmnI GCTCC 3 cut(s) 43, 79, 176
LweI GCATC 2 cut(s) 418, 668
MaeI CTAG 1 cut(s) 495
MaeII ACGT 5 cut(s) 129, 309, 315, 346, 756
MaeIII GTNAC 5 cut(s) 256, 334, 355, 490, 499
MalI GATC 1 cut(s) 776
MboI GATC 1 cut(s) 774
MboII GAAGA 3 cut(s) 202, 569, 689
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 4 cut(s) 65, 262, 270, 301
MmeI TCCRAC 4 cut(s) 367, 511, 550, 633
MnlI CCTC 3 cut(s) 237, 299, 722
Mph1103I ATGCAT 1 cut(s) 672
MseI TTAA 5 cut(s) 5, 68, 582, 771, 793
MslI CAYNNNNRTG 1 cut(s) 239
MspI CCGG 2 cut(s) 104, 365
MspR9I CCNGG 2 cut(s) 366, 513
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 1 cut(s) 786
NciI CCSGG 1 cut(s) 366
NcoI CCATGG 1 cut(s) 517
NdeII GATC 1 cut(s) 774
NlaIII CATG 3 cut(s) 521, 633, 691
NlaIV GGNNCC 4 cut(s) 39, 326, 363, 740
NmuCI GTSAC 2 cut(s) 334, 355
NsiI ATGCAT 1 cut(s) 672
PfeI GAWTC 5 cut(s) 49, 390, 414, 573, 621
PflFI GACNNNGTC 1 cut(s) 128
PflMI CCANNNNNTGG 1 cut(s) 518
PkrI GCNGC 1 cut(s) 89
PpuMI RGGWCCY 1 cut(s) 26
Psp1406I AACGTT 2 cut(s) 315, 756
Psp5II RGGWCCY 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 4 cut(s) 39, 326, 363, 740
PspPI GGNCC 2 cut(s) 26, 509
PspPPI RGGWCCY 1 cut(s) 26
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 2 cut(s) 57, 728
RsaNI GTAC 2 cut(s) 56, 727
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 5 cut(s) 5, 68, 582, 771, 793
SatI GCNGC 1 cut(s) 88
Sau3AI GATC 1 cut(s) 774
Sau96I GGNCC 2 cut(s) 26, 509
ScaI AGTACT 1 cut(s) 728
ScrFI CCNGG 2 cut(s) 366, 513
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 2 cut(s) 418, 668
SinI GGWCC 2 cut(s) 26, 509
SmiMI CAYNNNNRTG 1 cut(s) 239
Sse9I AATT 4 cut(s) 65, 262, 270, 301
SsiI CCGC 1 cut(s) 87
SspMI CTAG 1 cut(s) 495
StyD4I CCNGG 2 cut(s) 364, 511
StyI CCWWGG 2 cut(s) 172, 517
TaaI ACNGT 5 cut(s) 145, 455, 490, 614, 726
TaiI ACGT 5 cut(s) 132, 312, 318, 349, 759
TaqI TCGA 1 cut(s) 291
TasI AATT 4 cut(s) 65, 262, 270, 301
TatI WGTACW 2 cut(s) 55, 726
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 5 cut(s) 49, 390, 414, 573, 621
Tru1I TTAA 5 cut(s) 5, 68, 582, 771, 793
Tru9I TTAA 5 cut(s) 5, 68, 582, 771, 793
TscAI CASTG 1 cut(s) 749
TseFI GTSAC 2 cut(s) 334, 355
Tsp45I GTSAC 2 cut(s) 334, 355
TspDTI ATGAA 1 cut(s) 570
TspRI CASTG 1 cut(s) 749
Tth111I GACNNNGTC 1 cut(s) 128
Van91I CCANNNNNTGG 1 cut(s) 518
VpaK11BI GGWCC 2 cut(s) 26, 509
XapI RAATTY 1 cut(s) 301
XspI CTAG 1 cut(s) 495
ZrmI AGTACT 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.