MD13G1220200.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000434
Physical Location & Seq
Forward (+)
21312348 .. 21316197
3850 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1220200.v1.1.491

Sequence Viewer

Length: 1488 bp
ATGGCTCGAAAATTGAATGCCCTGGCAGTGTTTTTGTTCTTATTGTCAACATCGAATGCCAAAGCTGAGTTAGGTGTGTTTGATGTTACAAGTGCAACATATGGTGCAAAGCCCGGGTCTGATATCACACAGCCTTTAGCCAAGGCTTGGACTGATGCATGCGCATCCCCAACGACGAGTAAGGTTGTTGTTCCGAGCGGGACATACAAGTTAACAGAAGCAAGTTTCAAAGGTCCTTGCAAGGCTCCTATTGAGATTCAAGTTCAAGGCAAATTACAGGCTCCAGCAGATGGCGGCAAACTTACCAAACCAGATACTTGGGTTGCTTTTGAGCATATTAATATGCTCACCATCTACGGTGGTGGAACTTTTGATGGCCAAGGAGCACTCGCTTGGAAGCTAAATGACTGCAATAAAAACAAAGATTGCAAATCTATCGCCATTAATATGAGGTTCAACTTTGTTACCAATTCCACAATTCAAGACATAACTTCTCTCGATAGCAAGAATTTCCACGTCAACATTTTAGGGTGCCACAATGTCACATTCCAACATGTCAATGTCACAGCACCTGGAGAGAGCATAAATACGGATGGAATCCATATCGGGCGTTCAACTGGAATCAACATTACTGACGCACACATTGGAACTGGGGATGACTGTGTTTCCATTGGCGATGGAAGCAAAGAAATTACCGTAACTAGGGTTACTTGTGGACCAGGCCATGGCATAAGCGTTGGAAGTCTTGGAAGGTACCCGAACGAAGAACCTGTGGTTGGAATCAAAGTTAGGAACTGCACCTTGACTAATACATCCAACGGTGTGAGAATCAAGACATGGCCTGCTTCTCCTTCAGATGGTATTGCATCAGATATGCACTTTGAAGATATTATAGTGGTCAACGTCAGCAACCCTATCCTCATAGACCAACAGTACTGCCCGTGGAATCAATGTAAACAGAACATTCCGTCAAAAGTGAAGATCAGCAACGTCAGCTTCAAGAACATTAAGGGCTCATCTGCAACACCATTTGCAATCCAAATTATATGCAGCAAGGGCTTACCGTGTGAGAATGTGGAATTGTCTGACATTGATCTCACCTACAGCGGAAACAAAGGCACTCTTAACTCTCAATGTTCTAATGTGAAGCCCACCATTACTCGCGTGCTAAAAGCTCTTGCATGCGCTACCTCTTCATCTGGTACATCTGTTGAGTGGCTTGTTGAAGGTTCGTCCAATGAAGATTCTTTTAATGGTTTGGTACAAGATGGTGACACACATAAGTTCAAGAACATTAAGGGCTCATCTGCCACACCACTTGCAGTCCAAATTGTATGCAGCAAGGGCTTACCGTGTGAGAATGTGGAATTGTCTAACATTAATCTCACCTACAGCGGCAACAAATACCCTCTTACCTCTCAATGTTCTAACGTGAAGCCCACCATTACTCGCGTGCCAAATGCTCTTGCATGTGCTACCTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

496

Amino Acids

52.56

Weight (kDa)

8.03

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 54 - 383 7.4e-104 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 428 - 480 1.4e-08 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 163
Acc65I GGTACC 1 cut(s) 753
AccB1I GGYRCC 2 cut(s) 531, 753
AccB7I CCANNNNNTGG 3 cut(s) 147, 290, 725
AccBSI CCGCTC 1 cut(s) 198
AccII CGCG 2 cut(s) 1164, 1452
AciI CCGC 4 cut(s) 198, 294, 1107, 1395
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 1 cut(s) 508
AcuI CTGAAG 1 cut(s) 837
AfaI GTAC 4 cut(s) 755, 935, 1204, 1263
AfiI CCNNNNNNNGG 6 cut(s) 147, 290, 702, 725, 776, 857
AflIII ACRYGT 1 cut(s) 553
AjiI CACGTC 1 cut(s) 517
AjnI CCWGG 3 cut(s) 21, 571, 718
AjuI GAANNNNNNNTTGG 2 cut(s) 759, 791
AluBI AGCT 4 cut(s) 65, 400, 996, 1175
AluI AGCT 4 cut(s) 65, 400, 996, 1175
Alw21I GWGCWC 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 572
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 3 cut(s) 376, 721, 839
ApeKI GCWGC 2 cut(s) 1050, 1338
ApoI RAATTY 1 cut(s) 508
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AseI ATTAAT 3 cut(s) 339, 444, 1380
Asp718I GGTACC 1 cut(s) 753
AspLEI GCGC 2 cut(s) 164, 1187
AspS9I GGNCC 2 cut(s) 233, 716
AsuC2I CCSGG 2 cut(s) 114, 115
AsuHPI GGTGA 4 cut(s) 340, 1090, 1283, 1378
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 2 cut(s) 233, 716
BaeI ACNNNNGTAYC 4 cut(s) 306, 339, 1194, 1227
BalI TGGCCA 1 cut(s) 378
BanI GGYRCC 2 cut(s) 531, 753
BanII GRGCYC 2 cut(s) 1016, 1304
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 2 cut(s) 1062, 1350
BccI CCATC 7 cut(s) 284, 359, 368, 587, 671, 851, 1262
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BciT130I CCWGG 3 cut(s) 23, 573, 720
BcnI CCSGG 2 cut(s) 114, 115
BfaI CTAG 1 cut(s) 702
BfmI CTRYAG 2 cut(s) 1102, 1390
BisI GCNGC 4 cut(s) 295, 1051, 1339, 1396
BlsI GCNGC 4 cut(s) 296, 1052, 1340, 1397
BmcAI AGTACT 1 cut(s) 935
Bme1390I CCNGG 5 cut(s) 23, 114, 115, 573, 720
Bme18I GGWCC 2 cut(s) 233, 716
BmeT110I CYCGRG 1 cut(s) 113
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 2 cut(s) 233, 716
BmiI GGNNCC 4 cut(s) 246, 282, 533, 755
BmrFI CCNGG 5 cut(s) 23, 114, 115, 573, 720
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 3 cut(s) 145, 173, 875
BmuI ACTGGG 1 cut(s) 660
BpmI CTGGAG 2 cut(s) 267, 594
BpuMI CCSGG 2 cut(s) 114, 115
BsaJI CCNNGG 6 cut(s) 21, 113, 141, 379, 724, 941
Bsc4I CCNNNNNNNGG 6 cut(s) 147, 290, 702, 725, 776, 857
Bse1I ACTGG 2 cut(s) 622, 655
BseBI CCWGG 3 cut(s) 23, 573, 720
BseDI CCNNGG 6 cut(s) 21, 113, 141, 379, 724, 941
BseGI GGATG 4 cut(s) 164, 598, 661, 812
BseLI CCNNNNNNNGG 6 cut(s) 147, 290, 702, 725, 776, 857
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 2 cut(s) 622, 655
BseXI GCAGC 2 cut(s) 1062, 1350
BsgI GTGCAG 1 cut(s) 781
Bsh1236I CGCG 2 cut(s) 1164, 1452
BshFI GGCC 3 cut(s) 378, 723, 841
BshNI GGYRCC 2 cut(s) 531, 753
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 1 cut(s) 114
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 6 cut(s) 147, 290, 702, 725, 776, 857
BsmFI GGGAC 1 cut(s) 214
BsmI GAATGC 2 cut(s) 22, 61
BsnI GGCC 3 cut(s) 378, 723, 841
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 3 cut(s) 388, 1016, 1304
Bsp143I GATC 2 cut(s) 981, 1093
Bsp19I CCATGG 1 cut(s) 724
BspACI CCGC 4 cut(s) 198, 294, 1107, 1395
BspANI GGCC 3 cut(s) 378, 723, 841
BspCNI CTCAG 1 cut(s) 58
BspFNI CGCG 2 cut(s) 1164, 1452
BspLI GGNNCC 4 cut(s) 246, 282, 533, 755
BspT107I GGYRCC 2 cut(s) 531, 753
BsrBI CCGCTC 1 cut(s) 198
BsrI ACTGG 2 cut(s) 622, 655
BssECI CCNNGG 6 cut(s) 21, 113, 141, 379, 724, 941
BssMI GATC 2 cut(s) 981, 1093
BssT1I CCWWGG 3 cut(s) 141, 379, 724
Bst2UI CCWGG 3 cut(s) 23, 573, 720
Bst4CI ACNGT 7 cut(s) 359, 662, 697, 821, 933, 1065, 1353
Bst6I CTCTTC 1 cut(s) 1198
BstC8I GCNNGC 5 cut(s) 160, 843, 1166, 1183, 1454
BstDEI CTNAG 1 cut(s) 66
BstDSI CCRYGG 2 cut(s) 724, 941
BstF5I GGATG 4 cut(s) 164, 598, 661, 812
BstFNI CGCG 2 cut(s) 1164, 1452
BstHHI GCGC 2 cut(s) 164, 1187
BstKTI GATC 2 cut(s) 984, 1096
BstMBI GATC 2 cut(s) 981, 1093
BstMWI GCNNNNNNNGC 4 cut(s) 681, 993, 1056, 1344
BstNI CCWGG 3 cut(s) 23, 573, 720
BstNSI RCATGY 4 cut(s) 162, 557, 1185, 1473
BstSCI CCNGG 5 cut(s) 21, 112, 113, 571, 718
BstSFI CTRYAG 2 cut(s) 1102, 1390
BstUI CGCG 2 cut(s) 1164, 1452
BstV1I GCAGC 2 cut(s) 1062, 1350
BstXI CCANNNNNNTGG 1 cut(s) 318
BsuRI GGCC 3 cut(s) 378, 723, 841
BtgI CCRYGG 2 cut(s) 724, 941
BtgZI GCGATG 1 cut(s) 690
BtrI CACGTC 1 cut(s) 517
BtsCI GGATG 4 cut(s) 164, 598, 661, 812
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 5 cut(s) 160, 843, 1166, 1183, 1454
CaiI CAGNNNCTG 1 cut(s) 572
CfoI GCGC 2 cut(s) 164, 1187
Cfr13I GGNCC 2 cut(s) 233, 716
Cfr9I CCCGGG 1 cut(s) 113
CseI GACGC 1 cut(s) 644
Csp6I GTAC 4 cut(s) 754, 934, 1203, 1262
CspCI CAANNNNNGTGG 2 cut(s) 1300, 1335
CviAII CATG 6 cut(s) 159, 554, 725, 837, 1182, 1470
CviQI GTAC 4 cut(s) 754, 934, 1203, 1262
DdeI CTNAG 1 cut(s) 66
DpnI GATC 2 cut(s) 983, 1095
DpnII GATC 2 cut(s) 981, 1093
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 1198
EarI CTCTTC 1 cut(s) 1198
Eco130I CCWWGG 3 cut(s) 141, 379, 724
Eco24I GRGCYC 2 cut(s) 1016, 1304
Eco32I GATATC 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 233, 716
Eco57I CTGAAG 1 cut(s) 837
Eco88I CYCGRG 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 3 cut(s) 21, 571, 718
EcoRV GATATC 1 cut(s) 124
EcoT14I CCWWGG 3 cut(s) 141, 379, 724
EcoT22I ATGCAT 1 cut(s) 160
EcoT38I GRGCYC 2 cut(s) 1016, 1304
ErhI CCWWGG 3 cut(s) 141, 379, 724
FaeI CATG 6 cut(s) 162, 557, 728, 840, 1185, 1473
FalI AAGNNNNNCTT 2 cut(s) 1107, 1139
FaqI GGGAC 1 cut(s) 214
FatI CATG 6 cut(s) 158, 553, 724, 836, 1181, 1469
FauI CCCGC 1 cut(s) 191
FauNDI CATATG 1 cut(s) 100
Fnu4HI GCNGC 4 cut(s) 295, 1051, 1339, 1396
FokI GGATG 4 cut(s) 151, 605, 668, 799
FriOI GRGCYC 2 cut(s) 1016, 1304
Fsp4HI GCNGC 4 cut(s) 295, 1051, 1339, 1396
FspAI RTGCGCAY 1 cut(s) 163
FspBI CTAG 1 cut(s) 702
FspI TGCGCA 1 cut(s) 163
GlaI GCGC 2 cut(s) 163, 1186
GluI GCNGC 4 cut(s) 295, 1051, 1339, 1396
GsuI CTGGAG 2 cut(s) 267, 594
HaeIII GGCC 3 cut(s) 378, 723, 841
HapII CCGG 1 cut(s) 114
HgaI GACGC 1 cut(s) 644
HhaI GCGC 2 cut(s) 164, 1187
Hin1II CATG 6 cut(s) 162, 557, 728, 840, 1185, 1473
Hin6I GCGC 2 cut(s) 162, 1185
HinP1I GCGC 2 cut(s) 162, 1185
HincII GTYRAC 4 cut(s) 48, 213, 520, 901
HindII GTYRAC 4 cut(s) 48, 213, 520, 901
HinfI GANTC 7 cut(s) 256, 597, 621, 780, 828, 946, 1244
HpaI GTTAAC 1 cut(s) 213
HpaII CCGG 1 cut(s) 114
HphI GGTGA 4 cut(s) 340, 1090, 1283, 1378
Hpy166II GTNNAC 6 cut(s) 48, 213, 520, 716, 901, 956
Hpy188I TCNGA 5 cut(s) 121, 195, 856, 871, 1087
Hpy188III TCNNGA 6 cut(s) 482, 497, 832, 1000, 1288, 1485
Hpy8I GTNNAC 6 cut(s) 48, 213, 520, 716, 901, 956
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 3 cut(s) 744, 861, 1220
HpyCH4III ACNGT 7 cut(s) 359, 662, 697, 821, 933, 1065, 1353
HpyCH4IV ACGT 4 cut(s) 516, 903, 990, 1431
HpyF10VI GCNNNNNNNGC 4 cut(s) 681, 993, 1056, 1344
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 4 cut(s) 516, 903, 990, 1431
Hsp92II CATG 6 cut(s) 162, 557, 728, 840, 1185, 1473
HspAI GCGC 2 cut(s) 162, 1185
KpnI GGTACC 1 cut(s) 757
KspAI GTTAAC 1 cut(s) 213
Kzo9I GATC 2 cut(s) 981, 1093
LmnI GCTCC 3 cut(s) 250, 286, 383
Lsp1109I GCAGC 2 cut(s) 1062, 1350
LweI GCATC 3 cut(s) 145, 173, 875
MaeI CTAG 1 cut(s) 702
MaeII ACGT 4 cut(s) 516, 903, 990, 1431
MaeIII GTNAC 7 cut(s) 85, 463, 541, 562, 697, 706, 1271
MalI GATC 2 cut(s) 983, 1095
MbiI CCGCTC 1 cut(s) 198
MboI GATC 2 cut(s) 981, 1093
MboII GAAGA 6 cut(s) 776, 896, 991, 1185, 1253, 1473
MhlI GDGCHC 3 cut(s) 388, 1016, 1304
MlsI TGGCCA 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 378
MmeI TCCRAC 4 cut(s) 574, 718, 757, 840
MnlI CCTC 5 cut(s) 444, 929, 1201, 1419, 1426
Mox20I TGGCCA 1 cut(s) 378
Mph1103I ATGCAT 1 cut(s) 160
MscI TGGCCA 1 cut(s) 378
MseI TTAA 8 cut(s) 212, 339, 444, 1008, 1125, 1251, 1296, 1380
MslI CAYNNNNRTG 2 cut(s) 446, 558
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 2 cut(s) 1107, 1395
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 5 cut(s) 23, 114, 115, 573, 720
Mva1269I GAATGC 2 cut(s) 22, 61
MvaI CCWGG 3 cut(s) 23, 573, 720
MvnI CGCG 2 cut(s) 1164, 1452
MwoI GCNNNNNNNGC 4 cut(s) 681, 993, 1056, 1344
NciI CCSGG 2 cut(s) 114, 115
NcoI CCATGG 1 cut(s) 724
NdeI CATATG 1 cut(s) 100
NdeII GATC 2 cut(s) 981, 1093
NlaIII CATG 6 cut(s) 162, 557, 728, 840, 1185, 1473
NlaIV GGNNCC 4 cut(s) 246, 282, 533, 755
NmuCI GTSAC 3 cut(s) 541, 562, 1271
NsbI TGCGCA 1 cut(s) 163
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 4 cut(s) 162, 557, 1185, 1473
PaeI GCATGC 2 cut(s) 162, 1185
PciI ACATGT 1 cut(s) 553
PctI GAATGC 2 cut(s) 22, 61
PfeI GAWTC 7 cut(s) 256, 597, 621, 780, 828, 946, 1244
PflMI CCANNNNNTGG 3 cut(s) 147, 290, 725
PkrI GCNGC 4 cut(s) 296, 1052, 1340, 1397
PpuMI RGGWCCY 1 cut(s) 233
PscI ACATGT 1 cut(s) 553
PshBI ATTAAT 3 cut(s) 339, 444, 1380
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 3 cut(s) 21, 571, 718
PspGI CCWGG 3 cut(s) 21, 571, 718
PspN4I GGNNCC 4 cut(s) 246, 282, 533, 755
PspPI GGNCC 2 cut(s) 233, 716
PspPPI RGGWCCY 1 cut(s) 233
PstNI CAGNNNCTG 1 cut(s) 572
RsaI GTAC 4 cut(s) 755, 935, 1204, 1263
RsaNI GTAC 4 cut(s) 754, 934, 1203, 1262
RseI CAYNNNNRTG 2 cut(s) 446, 558
SaqAI TTAA 8 cut(s) 212, 339, 444, 1008, 1125, 1251, 1296, 1380
SatI GCNGC 4 cut(s) 295, 1051, 1339, 1396
Sau3AI GATC 2 cut(s) 981, 1093
Sau96I GGNCC 2 cut(s) 233, 716
ScaI AGTACT 1 cut(s) 935
ScrFI CCNGG 5 cut(s) 23, 114, 115, 573, 720
SduI GDGCHC 3 cut(s) 388, 1016, 1304
SfaNI GCATC 3 cut(s) 145, 173, 875
SfcI CTRYAG 2 cut(s) 1102, 1390
SinI GGWCC 2 cut(s) 233, 716
SmaI CCCGGG 1 cut(s) 115
SmiMI CAYNNNNRTG 2 cut(s) 446, 558
SphI GCATGC 2 cut(s) 162, 1185
SsiI CCGC 4 cut(s) 198, 294, 1107, 1395
SspMI CTAG 1 cut(s) 702
StyD4I CCNGG 5 cut(s) 21, 112, 113, 571, 718
StyI CCWWGG 3 cut(s) 141, 379, 724
TaaI ACNGT 7 cut(s) 359, 662, 697, 821, 933, 1065, 1353
TaiI ACGT 4 cut(s) 519, 906, 993, 1434
TaqI TCGA 3 cut(s) 7, 53, 498
TatI WGTACW 1 cut(s) 933
TauI GCSGC 2 cut(s) 297, 1398
TfiI GAWTC 7 cut(s) 256, 597, 621, 780, 828, 946, 1244
Tru1I TTAA 8 cut(s) 212, 339, 444, 1008, 1125, 1251, 1296, 1380
Tru9I TTAA 8 cut(s) 212, 339, 444, 1008, 1125, 1251, 1296, 1380
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 3 cut(s) 541, 562, 1271
TseI GCWGC 2 cut(s) 1050, 1338
Tsp45I GTSAC 3 cut(s) 541, 562, 1271
TspDTI ATGAA 2 cut(s) 1185, 1254
TspGWI ACGGA 2 cut(s) 605, 957
TspMI CCCGGG 1 cut(s) 113
TspRI CASTG 1 cut(s) 33
Van91I CCANNNNNTGG 3 cut(s) 147, 290, 725
VpaK11BI GGWCC 2 cut(s) 233, 716
VspI ATTAAT 3 cut(s) 339, 444, 1380
XapI RAATTY 1 cut(s) 508
XceI RCATGY 4 cut(s) 162, 557, 1185, 1473
XmaI CCCGGG 1 cut(s) 113
XspI CTAG 1 cut(s) 702
ZrmI AGTACT 1 cut(s) 935
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.