FvH4_7g02910

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000434
Physical Location & Seq
Reverse (-)
3232983 .. 3235112
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02910.t1

Sequence Viewer

Length: 1218 bp
ATGGGTCTCAAACTTGTCATTAATATGTTGTTAATACTTATGGCCATATCCTCGACATCAGCAAGTAGATCTAGGGAGCTTTTAGGCGGTGGTGTTTTCGACGTGACTAGTGGAAAATATGGTGCAAAGCCGAATTCTGATATCACTCAGGCTATAGCAAATGCATGGAAAGATGCATGTGCATCAACAATGCCAAGTAAAGTTATTGTTCCAAAGGGGATTTACAAATTGAAAGAAGCAAATTTCAACGGTCCTTGCAAGGCTCCTATTGAGCTTCAAGTCTGTGGCACTTTGCAAGCCTCACCAGACGGTGCCCAATTATCAAAACCAGATACCTGGATCATGTTTTCATACCTTGACAGGTTCACATTGTCAGGTACTGGAATTTTTGATGGCCAAGGACAACAAGCTTGGAAAGCTAATGATTGCCACAAAAACCCCAAGTGTTCATCCATTGCCATTAATATGAGGTTCCACTCTGTTAAGAACTCCTTAGTCAGAGATGTGAGTTCACTTAACAGCAAGAACTTCCACGTAAATGTACTAGGATGCGAGCGACTTACATTCCAACATTTCACCGTCACTGCACCAGAAACTAGCGTCAATACAGATGGAATACATATTGGCCGCTCAACCGGGATCAACATTACTGATTCCAATATTTCTACAGGAGATGACTGCATTTCAATTGGAGATGGCACCAAGCAACTAACTATAACTAAGGTGACTTGTGGACCAGGCCATGGAATAAGCATAGGAAGTTTGGGAAGGTACCCGAAAGAGGAACCTGTGACTGGAATCTATATTAAGAGCTGCACCCTTAGTAATACAATGAATGGTATTAGAATCAAGACATGGCCTAATTCTCCGGCAGCTAGCATAGCCTCAGATATTCACTTTGAGGACATAATCATGAATAATGTTGGTAACCCGATCCTCATAGATCAAGAATACTGCCCTTGGAATCTCTGTAATAAGCAGGTTGCATCAAAAGTGAAGATCAGCAACGTGAGCTTCAGGAACATTAGAGGGACGTCTTCCACTCCGTTGGCCTTGAAGATATTATGCGCCAAGGGCTTACCTTGTGACAAGGTGGAGATGACTGACATCAATCTCAAGTACAATGGAAAGCAAGGTTCACTTACATCTCAATGCTCTAATGTGAGGCCAACAATTACTCGTGTGCCAAATCCTCTTGCTTGTGCTACCAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

406

Amino Acids

43.58

Weight (kDa)

9.08

Isoelectric Point (pI)

30.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 60 - 389 5.1e-104 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1037
Acc36I ACCTGC 1 cut(s) 970
Acc65I GGTACC 1 cut(s) 771
AccB1I GGYRCC 3 cut(s) 311, 698, 771
AccB7I CCANNNNNTGG 1 cut(s) 743
AccBSI CCGCTC 1 cut(s) 630
AciI CCGC 2 cut(s) 87, 628
AclWI GGATC 3 cut(s) 347, 647, 928
AcoI YGGCCR 3 cut(s) 42, 394, 625
AcsI RAATTY 3 cut(s) 133, 241, 384
AcuI CTGAAG 1 cut(s) 1000
AcyI GRCGYC 1 cut(s) 1034
AfaI GTAC 4 cut(s) 379, 543, 773, 1121
AfiI CCNNNNNNNGG 3 cut(s) 743, 781, 794
AgsI TTSAA 5 cut(s) 232, 247, 278, 687, 1057
AhlI ACTAGT 1 cut(s) 107
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 2 cut(s) 335, 736
AluBI AGCT 7 cut(s) 79, 274, 410, 419, 813, 875, 1014
AluI AGCT 7 cut(s) 79, 274, 410, 419, 813, 875, 1014
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 3 cut(s) 347, 647, 928
AlwNI CAGNNNCTG 1 cut(s) 380
AoxI GGCC 7 cut(s) 42, 394, 625, 739, 857, 1050, 1166
ApeKI GCWGC 2 cut(s) 813, 872
ApoI RAATTY 3 cut(s) 133, 241, 384
AseI ATTAAT 2 cut(s) 21, 462
Asp718I GGTACC 1 cut(s) 771
AspLEI GCGC 1 cut(s) 1070
AspS9I GGNCC 2 cut(s) 251, 734
AsuC2I CCSGG 1 cut(s) 637
AsuHPI GGTGA 3 cut(s) 294, 568, 736
AsuNHI GCTAGC 1 cut(s) 875
AvaII GGWCC 2 cut(s) 251, 734
BaeGI GKGCMC 1 cut(s) 316
BalI TGGCCA 2 cut(s) 44, 396
BanI GGYRCC 3 cut(s) 311, 698, 771
BauI CACGAG 1 cut(s) 1179
BbsI GAAGAC 1 cut(s) 1029
BbvI GCAGC 2 cut(s) 800, 884
BccI CCATC 3 cut(s) 386, 605, 689
BciT130I CCWGG 2 cut(s) 337, 738
BcnI CCSGG 1 cut(s) 637
BcoDI GTCTC 1 cut(s) 11
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 5 cut(s) 72, 108, 545, 597, 876
BfmI CTRYAG 2 cut(s) 153, 666
BfuAI ACCTGC 1 cut(s) 970
BglII AGATCT 1 cut(s) 68
BisI GCNGC 3 cut(s) 628, 814, 873
BlsI GCNGC 3 cut(s) 629, 815, 874
Bme1390I CCNGG 3 cut(s) 337, 637, 738
Bme18I GGWCC 2 cut(s) 251, 734
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 251, 734
BmiI GGNNCC 6 cut(s) 264, 313, 473, 700, 773, 786
BmrFI CCNGG 3 cut(s) 337, 637, 738
BmsI GCATC 4 cut(s) 163, 191, 539, 995
BmtI GCTAGC 1 cut(s) 879
BpiI GAAGAC 1 cut(s) 1029
BpuEI CTTGAG 1 cut(s) 1100
BpuMI CCSGG 1 cut(s) 637
BsaAI YACGTR 1 cut(s) 535
BsaHI GRCGYC 1 cut(s) 1034
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 397, 742, 959, 1071
Bsc4I CCNNNNNNNGG 3 cut(s) 743, 781, 794
Bse1I ACTGG 2 cut(s) 385, 799
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 2 cut(s) 337, 738
BseDI CCNNGG 4 cut(s) 397, 742, 959, 1071
BseGI GGATG 2 cut(s) 449, 554
BseLI CCNNNNNNNGG 3 cut(s) 743, 781, 794
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 2 cut(s) 161, 900
BseNI ACTGG 2 cut(s) 385, 799
BseSI GKGCMC 1 cut(s) 316
BseXI GCAGC 2 cut(s) 800, 884
BsgI GTGCAG 2 cut(s) 570, 799
BshFI GGCC 7 cut(s) 44, 396, 627, 741, 859, 1052, 1168
BshNI GGYRCC 3 cut(s) 311, 698, 771
BsiSI CCGG 2 cut(s) 636, 869
BslFI GGGAC 1 cut(s) 1045
BslI CCNNNNNNNGG 3 cut(s) 743, 781, 794
BsmAI GTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 1045
BsnI GGCC 7 cut(s) 44, 396, 627, 741, 859, 1052, 1168
Bso31I GGTCTC 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 6 cut(s) 68, 339, 639, 933, 943, 999
Bsp19I CCATGG 1 cut(s) 742
BspACI CCGC 2 cut(s) 87, 628
BspANI GGCC 7 cut(s) 44, 396, 627, 741, 859, 1052, 1168
BspCNI CTCAG 2 cut(s) 160, 899
BspHI TCATGA 1 cut(s) 912
BspLI GGNNCC 6 cut(s) 264, 313, 473, 700, 773, 786
BspMI ACCTGC 1 cut(s) 970
BspOI GCTAGC 1 cut(s) 879
BspPI GGATC 3 cut(s) 347, 647, 928
BspT107I GGYRCC 3 cut(s) 311, 698, 771
BspTNI GGTCTC 1 cut(s) 11
BsrBI CCGCTC 1 cut(s) 630
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 2 cut(s) 385, 799
BssECI CCNNGG 4 cut(s) 397, 742, 959, 1071
BssMI GATC 6 cut(s) 68, 339, 639, 933, 943, 999
BssNI GRCGYC 1 cut(s) 1034
BssSI CACGAG 1 cut(s) 1179
BssT1I CCWWGG 4 cut(s) 397, 742, 959, 1071
Bst2BI CACGAG 1 cut(s) 1179
Bst2UI CCWGG 2 cut(s) 337, 738
Bst4CI ACNGT 3 cut(s) 251, 311, 580
BstACI GRCGYC 1 cut(s) 1034
BstBAI YACGTR 1 cut(s) 535
BstC8I GCNNGC 3 cut(s) 297, 554, 877
BstDEI CTNAG 5 cut(s) 147, 493, 720, 821, 886
BstDSI CCRYGG 1 cut(s) 742
BstEII GGTNACC 1 cut(s) 926
BstF5I GGATG 2 cut(s) 449, 554
BstHHI GCGC 1 cut(s) 1070
BstKTI GATC 6 cut(s) 71, 342, 642, 936, 946, 1002
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 6 cut(s) 68, 339, 639, 933, 943, 999
BstMWI GCNNNNNNNGC 4 cut(s) 416, 881, 1011, 1074
BstNI CCWGG 2 cut(s) 337, 738
BstNSI RCATGY 1 cut(s) 180
BstPI GGTNACC 1 cut(s) 926
BstSCI CCNGG 3 cut(s) 335, 635, 736
BstSFI CTRYAG 2 cut(s) 153, 666
BstSLI GKGCMC 1 cut(s) 316
BstV1I GCAGC 2 cut(s) 800, 884
BstV2I GAAGAC 1 cut(s) 1029
BstX2I RGATCY 1 cut(s) 68
BstXI CCANNNNNNTGG 2 cut(s) 336, 1048
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 7 cut(s) 44, 396, 627, 741, 859, 1052, 1168
BtgI CCRYGG 1 cut(s) 742
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 2 cut(s) 449, 554
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 1 cut(s) 582
BveI ACCTGC 1 cut(s) 970
Cac8I GCNNGC 3 cut(s) 297, 554, 877
CaiI CAGNNNCTG 1 cut(s) 380
CciI TCATGA 1 cut(s) 912
CfoI GCGC 1 cut(s) 1070
Cfr13I GGNCC 2 cut(s) 251, 734
CseI GACGC 1 cut(s) 589
Csp6I GTAC 4 cut(s) 378, 542, 772, 1120
CviAII CATG 6 cut(s) 165, 177, 343, 743, 855, 913
CviQI GTAC 4 cut(s) 378, 542, 772, 1120
DdeI CTNAG 5 cut(s) 147, 493, 720, 821, 886
DpnI GATC 6 cut(s) 70, 341, 641, 935, 945, 1001
DpnII GATC 6 cut(s) 68, 339, 639, 933, 943, 999
EaeI YGGCCR 3 cut(s) 42, 394, 625
Eco130I CCWWGG 4 cut(s) 397, 742, 959, 1071
Eco31I GGTCTC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 251, 734
Eco57I CTGAAG 1 cut(s) 1000
Eco91I GGTNACC 1 cut(s) 926
EcoO65I GGTNACC 1 cut(s) 926
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 2 cut(s) 335, 736
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 4 cut(s) 397, 742, 959, 1071
EcoT22I ATGCAT 2 cut(s) 166, 178
ErhI CCWWGG 4 cut(s) 397, 742, 959, 1071
FaeI CATG 6 cut(s) 168, 180, 346, 746, 858, 916
FalI AAGNNNNNCTT 4 cut(s) 476, 508, 1125, 1157
FaqI GGGAC 1 cut(s) 1045
FatI CATG 6 cut(s) 164, 176, 342, 742, 854, 912
Fnu4HI GCNGC 3 cut(s) 628, 814, 873
FokI GGATG 2 cut(s) 436, 561
Fsp4HI GCNGC 3 cut(s) 628, 814, 873
FspBI CTAG 5 cut(s) 72, 108, 545, 597, 876
GlaI GCGC 1 cut(s) 1069
GluI GCNGC 3 cut(s) 628, 814, 873
HaeIII GGCC 7 cut(s) 44, 396, 627, 741, 859, 1052, 1168
HapII CCGG 2 cut(s) 636, 869
HgaI GACGC 1 cut(s) 589
HhaI GCGC 1 cut(s) 1070
Hin1I GRCGYC 1 cut(s) 1034
Hin1II CATG 6 cut(s) 168, 180, 346, 746, 858, 916
Hin6I GCGC 1 cut(s) 1068
HinP1I GCGC 1 cut(s) 1068
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 4 cut(s) 653, 798, 846, 964
HpaII CCGG 2 cut(s) 636, 869
HphI GGTGA 3 cut(s) 294, 568, 736
Hpy166II GTNNAC 4 cut(s) 366, 512, 734, 1139
Hpy188I TCNGA 3 cut(s) 139, 500, 889
Hpy188III TCNNGA 5 cut(s) 850, 913, 947, 1018, 1215
Hpy8I GTNNAC 4 cut(s) 366, 512, 734, 1139
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 1 cut(s) 762
HpyCH4III ACNGT 3 cut(s) 251, 311, 580
HpyCH4IV ACGT 4 cut(s) 102, 534, 1008, 1034
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 881, 1011, 1074
HpyF3I CTNAG 5 cut(s) 147, 493, 720, 821, 886
HpySE526I ACGT 4 cut(s) 102, 534, 1008, 1034
Hsp92I GRCGYC 1 cut(s) 1034
Hsp92II CATG 6 cut(s) 168, 180, 346, 746, 858, 916
HspAI GCGC 1 cut(s) 1068
KpnI GGTACC 1 cut(s) 775
Kzo9I GATC 6 cut(s) 68, 339, 639, 933, 943, 999
LmnI GCTCC 2 cut(s) 76, 268
Lsp1109I GCAGC 2 cut(s) 800, 884
LweI GCATC 4 cut(s) 163, 191, 539, 995
MaeI CTAG 5 cut(s) 72, 108, 545, 597, 876
MaeII ACGT 4 cut(s) 102, 534, 1008, 1034
MaeIII GTNAC 6 cut(s) 103, 580, 724, 790, 926, 1085
MalI GATC 6 cut(s) 70, 341, 641, 935, 945, 1001
MbiI CCGCTC 1 cut(s) 630
MboI GATC 6 cut(s) 68, 339, 639, 933, 943, 999
MboII GAAGA 3 cut(s) 1009, 1029, 1069
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 1 cut(s) 316
MlsI TGGCCA 2 cut(s) 44, 396
MluCI AATT 8 cut(s) 133, 227, 241, 317, 384, 687, 862, 1173
MluNI TGGCCA 2 cut(s) 44, 396
MmeI TCCRAC 1 cut(s) 592
Mox20I TGGCCA 2 cut(s) 44, 396
Mph1103I ATGCAT 2 cut(s) 166, 178
MscI TGGCCA 2 cut(s) 44, 396
MseI TTAA 6 cut(s) 21, 32, 462, 483, 516, 807
MslI CAYNNNNRTG 5 cut(s) 23, 464, 537, 911, 1150
Msp20I TGGCCA 2 cut(s) 44, 396
MspI CCGG 2 cut(s) 636, 869
MspR9I CCNGG 3 cut(s) 337, 637, 738
MunI CAATTG 1 cut(s) 687
MvaI CCWGG 2 cut(s) 337, 738
MwoI GCNNNNNNNGC 4 cut(s) 416, 881, 1011, 1074
NciI CCSGG 1 cut(s) 637
NcoI CCATGG 1 cut(s) 742
NdeII GATC 6 cut(s) 68, 339, 639, 933, 943, 999
NheI GCTAGC 1 cut(s) 875
NlaIII CATG 6 cut(s) 168, 180, 346, 746, 858, 916
NlaIV GGNNCC 6 cut(s) 264, 313, 473, 700, 773, 786
NmuCI GTSAC 5 cut(s) 103, 580, 724, 790, 1085
NsiI ATGCAT 2 cut(s) 166, 178
NspI RCATGY 1 cut(s) 180
PagI TCATGA 1 cut(s) 912
PfeI GAWTC 4 cut(s) 653, 798, 846, 964
PflMI CCANNNNNTGG 1 cut(s) 743
PkrI GCNGC 3 cut(s) 629, 815, 874
Ppu21I YACGTR 1 cut(s) 535
PshBI ATTAAT 2 cut(s) 21, 462
Psp6I CCWGG 2 cut(s) 335, 736
PspEI GGTNACC 1 cut(s) 926
PspGI CCWGG 2 cut(s) 335, 736
PspN4I GGNNCC 6 cut(s) 264, 313, 473, 700, 773, 786
PspPI GGNCC 2 cut(s) 251, 734
PstNI CAGNNNCTG 1 cut(s) 380
PsuI RGATCY 1 cut(s) 68
RsaI GTAC 4 cut(s) 379, 543, 773, 1121
RsaNI GTAC 4 cut(s) 378, 542, 772, 1120
RseI CAYNNNNRTG 5 cut(s) 23, 464, 537, 911, 1150
SaqAI TTAA 6 cut(s) 21, 32, 462, 483, 516, 807
SatI GCNGC 3 cut(s) 628, 814, 873
Sau3AI GATC 6 cut(s) 68, 339, 639, 933, 943, 999
Sau96I GGNCC 2 cut(s) 251, 734
ScrFI CCNGG 3 cut(s) 337, 637, 738
SduI GDGCHC 1 cut(s) 316
SfaNI GCATC 4 cut(s) 163, 191, 539, 995
SfcI CTRYAG 2 cut(s) 153, 666
SinI GGWCC 2 cut(s) 251, 734
SmiMI CAYNNNNRTG 5 cut(s) 23, 464, 537, 911, 1150
SmlI CTYRAG 1 cut(s) 1115
SmoI CTYRAG 1 cut(s) 1115
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 8 cut(s) 133, 227, 241, 317, 384, 687, 862, 1173
SsiI CCGC 2 cut(s) 87, 628
SspI AATATT 1 cut(s) 661
SspMI CTAG 5 cut(s) 72, 108, 545, 597, 876
StyD4I CCNGG 3 cut(s) 335, 635, 736
StyI CCWWGG 4 cut(s) 397, 742, 959, 1071
TaaI ACNGT 3 cut(s) 251, 311, 580
TaiI ACGT 4 cut(s) 105, 537, 1011, 1037
TaqI TCGA 2 cut(s) 53, 99
TasI AATT 8 cut(s) 133, 227, 241, 317, 384, 687, 862, 1173
TatI WGTACW 2 cut(s) 541, 1119
TauI GCSGC 1 cut(s) 630
TfiI GAWTC 4 cut(s) 653, 798, 846, 964
Tru1I TTAA 6 cut(s) 21, 32, 462, 483, 516, 807
Tru9I TTAA 6 cut(s) 21, 32, 462, 483, 516, 807
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 5 cut(s) 103, 580, 724, 790, 1085
TseI GCWGC 2 cut(s) 813, 872
Tsp45I GTSAC 5 cut(s) 103, 580, 724, 790, 1085
TspDTI ATGAA 4 cut(s) 339, 438, 848, 929
TspGWI ACGGA 1 cut(s) 1035
TspRI CASTG 1 cut(s) 589
Van91I CCANNNNNTGG 1 cut(s) 743
VpaK11BI GGWCC 2 cut(s) 251, 734
VspI ATTAAT 2 cut(s) 21, 462
XapI RAATTY 3 cut(s) 133, 241, 384
XceI RCATGY 1 cut(s) 180
XspI CTAG 5 cut(s) 72, 108, 545, 597, 876
ZraI GACGTC 1 cut(s) 1035
Zsp2I ATGCAT 2 cut(s) 166, 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.