Rmu_ssc0000408.1_g000005

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000408.1
OG0000434
Physical Location & Seq
Forward (+)
21555 .. 24119
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000408.1_g000005.1.cds

Sequence Viewer

Length: 1179 bp
atgcagagagagcttctagccgatggtggtgactttgacgtgaccaacgcgaaatatggtgcaaagcccgattctgatattactgaggctttagcaaatgcttggaaagacgcctgtgcatcaacatcggcaagtaaagttattgttccgaaagggacattcaaagtaaaagaagcaagtttcaaaggtccttgcaaggctcctattgaggttcaggtccaaggcacattacaggcgccagaagactcaagccaattatcgaaacaagatacttggatcgagtttttatacctcaacgacttcacactatcgggtggtggaacctttgatggccaaggacaaaaaacttggaaagccattgatccaaaaaacgcaaaggctggcaccattaatatcaggttccacgccgtcaagaagtcattagttaaggatataacttcacttaatagcaagaacttccacatcaacgtaataagttgcgagcaacttacgttcgatcatgtcaccgtcactgcacctggagatagccctaacacagatggaatacatatcgcgcgctcaaccggggtcaacgttactgatacaaatatcggaaccggagatgattgcatttcaattggtgatggtaccaagcaacttacaataactaaggttacttgtggaccaggccatggaataagcgttggaagtcttggaaggtacgacaaagaagaccctgtggaaggaatcaatgttaagaactgtaccctcagtaaaacgactaatggtgttagaatcaagacatgggctaaaaatcctcaagctagcacttgctcagaaattcactttgaggatatcatcatgaatgatgttcaaaacccaatcgtcgtagatcaagaatactgcccatgggatcaatgtgataaacaggcttcatcgaaaatcaagatcagcgatgtgagtttcaagaacattagagggacgacttccactccgttgggcgtgaagattgtatgcgccaagggcttaccatgtgagaaggtggagatgactggcattgatctcaaattcaccggcacaggtgcactcacgtctcaatgtacgaatgtcaaacccacaattgctaatgtggcacagcctcttgcttgtgctaccgatgctcctgtacaggctggaccagctgagaagtctaatgactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

392

Amino Acids

41.79

Weight (kDa)

5.93

Isoelectric Point (pI)

26.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 626
AccB1I GGYRCC 3 cut(s) 235, 383, 626
AccB7I CCANNNNNTGG 1 cut(s) 671
AccII CGCG 3 cut(s) 50, 554, 556
AclI AACGTT 1 cut(s) 573
AclWI GGATC 3 cut(s) 284, 356, 900
AcoI YGGCCR 1 cut(s) 331
AcsI RAATTY 2 cut(s) 819, 1046
AcyI GRCGYC 2 cut(s) 111, 236
AfaI GTAC 5 cut(s) 628, 701, 745, 1081, 1146
AfiI CCNNNNNNNGG 2 cut(s) 671, 722
AgsI TTSAA 5 cut(s) 163, 184, 615, 854, 946
AjiI CACGTC 2 cut(s) 40, 1071
AjnI CCWGG 2 cut(s) 517, 664
AluBI AGCT 3 cut(s) 13, 803, 1160
AluI AGCT 3 cut(s) 13, 803, 1160
Alw21I GWGCWC 1 cut(s) 1066
Alw26I GTCTC 1 cut(s) 1077
Alw44I GTGCAC 1 cut(s) 1062
AlwI GGATC 3 cut(s) 284, 356, 900
AoxI GGCC 2 cut(s) 331, 667
ApaLI GTGCAC 1 cut(s) 1062
ApoI RAATTY 2 cut(s) 819, 1046
AseI ATTAAT 1 cut(s) 390
Asp718I GGTACC 1 cut(s) 626
AspLEI GCGC 4 cut(s) 238, 556, 558, 998
AspS9I GGNCC 4 cut(s) 188, 217, 662, 1154
AsuC2I CCSGG 1 cut(s) 565
AsuHPI GGTGA 4 cut(s) 41, 496, 632, 1042
AsuNHI GCTAGC 1 cut(s) 803
AvaII GGWCC 4 cut(s) 188, 217, 662, 1154
BaeGI GKGCMC 1 cut(s) 1066
BalI TGGCCA 1 cut(s) 333
BanI GGYRCC 3 cut(s) 235, 383, 626
BbsI GAAGAC 2 cut(s) 249, 717
Bbv12I GWGCWC 1 cut(s) 1066
BccI CCATC 4 cut(s) 17, 323, 533, 617
BceAI ACGGC 1 cut(s) 392
BcgI CGANNNNNNTGC 2 cut(s) 108, 142
BciT130I CCWGG 2 cut(s) 519, 666
BcnI CCSGG 1 cut(s) 565
BcoDI GTCTC 1 cut(s) 1077
BfaI CTAG 2 cut(s) 17, 804
BfoI RGCGCY 1 cut(s) 239
Bme1390I CCNGG 3 cut(s) 519, 565, 666
Bme18I GGWCC 4 cut(s) 188, 217, 662, 1154
BmgBI CACGTC 2 cut(s) 40, 1071
BmgT120I GGNCC 4 cut(s) 188, 217, 662, 1154
BmiI GGNNCC 7 cut(s) 201, 237, 322, 385, 401, 595, 628
BmrFI CCNGG 3 cut(s) 519, 565, 666
BmsI GCATC 2 cut(s) 128, 1126
BmtI GCTAGC 1 cut(s) 807
BpiI GAAGAC 2 cut(s) 249, 717
BpmI CTGGAG 1 cut(s) 540
BpuEI CTTGAG 2 cut(s) 232, 783
BpuMI CCSGG 1 cut(s) 565
BsaHI GRCGYC 2 cut(s) 111, 236
BsaJI CCNNGG 6 cut(s) 220, 334, 564, 670, 887, 999
BsaWI WCCGGW 1 cut(s) 596
BsaXI ACNNNNNCTCC 4 cut(s) 955, 985, 1123, 1153
Bsc4I CCNNNNNNNGG 2 cut(s) 671, 722
Bse118I RCCGGY 1 cut(s) 1052
Bse1I ACTGG 1 cut(s) 1036
BseBI CCWGG 2 cut(s) 519, 666
BseDI CCNNGG 6 cut(s) 220, 334, 564, 670, 887, 999
BseLI CCNNNNNNNGG 2 cut(s) 671, 722
BseMII CTCAG 4 cut(s) 75, 763, 828, 1152
BseNI ACTGG 1 cut(s) 1036
BsePI GCGCGC 1 cut(s) 554
BseSI GKGCMC 1 cut(s) 1066
BsgI GTGCAG 1 cut(s) 498
Bsh1236I CGCG 3 cut(s) 50, 554, 556
BshFI GGCC 2 cut(s) 333, 669
BshNI GGYRCC 3 cut(s) 235, 383, 626
BsiHKAI GWGCWC 1 cut(s) 1066
BsiSI CCGG 3 cut(s) 564, 597, 1053
BslFI GGGAC 2 cut(s) 169, 973
BslI CCNNNNNNNGG 2 cut(s) 671, 722
BsmAI GTCTC 1 cut(s) 1077
BsmBI CGTCTC 1 cut(s) 1077
BsmFI GGGAC 2 cut(s) 169, 973
BsnI GGCC 2 cut(s) 333, 669
Bsp1286I GDGCHC 1 cut(s) 1066
Bsp1407I TGTACA 1 cut(s) 1144
Bsp143I GATC 7 cut(s) 276, 361, 496, 871, 892, 927, 1039
Bsp19I CCATGG 2 cut(s) 670, 887
BspANI GGCC 2 cut(s) 333, 669
BspCNI CTCAG 4 cut(s) 76, 762, 827, 1153
BspFNI CGCG 3 cut(s) 50, 554, 556
BspHI TCATGA 1 cut(s) 840
BspLI GGNNCC 7 cut(s) 201, 237, 322, 385, 401, 595, 628
BspOI GCTAGC 1 cut(s) 807
BspPI GGATC 3 cut(s) 284, 356, 900
BspT107I GGYRCC 3 cut(s) 235, 383, 626
BsrFI RCCGGY 1 cut(s) 1052
BsrGI TGTACA 1 cut(s) 1144
BsrI ACTGG 1 cut(s) 1036
BssAI RCCGGY 1 cut(s) 1052
BssECI CCNNGG 6 cut(s) 220, 334, 564, 670, 887, 999
BssHII GCGCGC 1 cut(s) 554
BssMI GATC 7 cut(s) 276, 361, 496, 871, 892, 927, 1039
BssNI GRCGYC 2 cut(s) 111, 236
BssT1I CCWWGG 5 cut(s) 220, 334, 670, 887, 999
Bst2UI CCWGG 2 cut(s) 519, 666
Bst4CI ACNGT 2 cut(s) 508, 743
BstACI GRCGYC 2 cut(s) 111, 236
BstAUI TGTACA 1 cut(s) 1144
BstC8I GCNNGC 4 cut(s) 382, 482, 556, 805
BstDEI CTNAG 5 cut(s) 84, 648, 749, 814, 1161
BstDSI CCRYGG 2 cut(s) 670, 887
BstENI CCTNNNNNAGG 1 cut(s) 720
BstFNI CGCG 3 cut(s) 50, 554, 556
BstH2I RGCGCY 1 cut(s) 239
BstHHI GCGC 4 cut(s) 238, 556, 558, 998
BstKTI GATC 7 cut(s) 279, 364, 499, 874, 895, 930, 1042
BstMAI GTCTC 1 cut(s) 1077
BstMBI GATC 7 cut(s) 276, 361, 496, 871, 892, 927, 1039
BstMWI GCNNNNNNNGC 5 cut(s) 10, 1002, 1109, 1136, 1157
BstNI CCWGG 2 cut(s) 519, 666
BstSCI CCNGG 3 cut(s) 517, 563, 664
BstSLI GKGCMC 1 cut(s) 1066
BstUI CGCG 3 cut(s) 50, 554, 556
BstV2I GAAGAC 2 cut(s) 249, 717
BstXI CCANNNNNNTGG 1 cut(s) 976
BsuRI GGCC 2 cut(s) 333, 669
BtgI CCRYGG 2 cut(s) 670, 887
BtgZI GCGATG 1 cut(s) 948
BtrI CACGTC 2 cut(s) 40, 1071
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 1 cut(s) 510
Cac8I GCNNGC 4 cut(s) 382, 482, 556, 805
CciI TCATGA 1 cut(s) 840
CfoI GCGC 4 cut(s) 238, 556, 558, 998
Cfr10I RCCGGY 1 cut(s) 1052
Cfr13I GGNCC 4 cut(s) 188, 217, 662, 1154
CseI GACGC 1 cut(s) 119
Csp6I GTAC 5 cut(s) 627, 700, 744, 1080, 1145
CviAII CATG 6 cut(s) 500, 671, 783, 841, 888, 1011
CviQI GTAC 5 cut(s) 627, 700, 744, 1080, 1145
DdeI CTNAG 5 cut(s) 84, 648, 749, 814, 1161
DinI GGCGCC 1 cut(s) 237
DpnI GATC 7 cut(s) 278, 363, 498, 873, 894, 929, 1041
DpnII GATC 7 cut(s) 276, 361, 496, 871, 892, 927, 1039
EaeI YGGCCR 1 cut(s) 331
Eco130I CCWWGG 5 cut(s) 220, 334, 670, 887, 999
Eco32I GATATC 1 cut(s) 835
Eco47I GGWCC 4 cut(s) 188, 217, 662, 1154
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoO109I RGGNCCY 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 517, 664
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 5 cut(s) 220, 334, 670, 887, 999
EgeI GGCGCC 1 cut(s) 237
EheI GGCGCC 1 cut(s) 237
ErhI CCWWGG 5 cut(s) 220, 334, 670, 887, 999
Esp3I CGTCTC 1 cut(s) 1077
FaeI CATG 6 cut(s) 503, 674, 786, 844, 891, 1014
FaqI GGGAC 2 cut(s) 169, 973
FatI CATG 6 cut(s) 499, 670, 782, 840, 887, 1010
FspBI CTAG 2 cut(s) 17, 804
GlaI GCGC 4 cut(s) 237, 555, 557, 997
GsuI CTGGAG 1 cut(s) 540
HaeII RGCGCY 1 cut(s) 239
HaeIII GGCC 2 cut(s) 333, 669
HapII CCGG 3 cut(s) 564, 597, 1053
HgaI GACGC 1 cut(s) 119
HhaI GCGC 4 cut(s) 238, 556, 558, 998
Hin1I GRCGYC 2 cut(s) 111, 236
Hin1II CATG 6 cut(s) 503, 674, 786, 844, 891, 1014
Hin6I GCGC 4 cut(s) 236, 554, 556, 996
HinP1I GCGC 4 cut(s) 236, 554, 556, 996
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 4 cut(s) 71, 245, 726, 774
HpaII CCGG 3 cut(s) 564, 597, 1053
HphI GGTGA 4 cut(s) 41, 496, 632, 1042
Hpy166II GTNNAC 3 cut(s) 571, 662, 1064
Hpy188I TCNGA 4 cut(s) 76, 150, 593, 817
Hpy188III TCNNGA 6 cut(s) 412, 778, 841, 875, 925, 946
Hpy8I GTNNAC 3 cut(s) 571, 662, 1064
Hpy99I CGWCG 1 cut(s) 869
HpyAV CCTTC 3 cut(s) 690, 716, 1012
HpyCH4III ACNGT 2 cut(s) 508, 743
HpyCH4IV ACGT 5 cut(s) 39, 468, 491, 573, 1070
HpyCH4V TGCA 7 cut(s) 4, 62, 119, 195, 515, 609, 1064
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 1002, 1109, 1136, 1157
HpyF3I CTNAG 5 cut(s) 84, 648, 749, 814, 1161
HpySE526I ACGT 5 cut(s) 39, 468, 491, 573, 1070
Hsp92I GRCGYC 2 cut(s) 111, 236
Hsp92II CATG 6 cut(s) 503, 674, 786, 844, 891, 1014
HspAI GCGC 4 cut(s) 236, 554, 556, 996
KasI GGCGCC 1 cut(s) 235
KpnI GGTACC 1 cut(s) 630
Kzo9I GATC 7 cut(s) 276, 361, 496, 871, 892, 927, 1039
LmnI GCTCC 2 cut(s) 205, 1144
LweI GCATC 2 cut(s) 128, 1126
MaeI CTAG 2 cut(s) 17, 804
MaeII ACGT 5 cut(s) 39, 468, 491, 573, 1070
MaeIII GTNAC 6 cut(s) 29, 40, 502, 508, 574, 652
MalI GATC 7 cut(s) 278, 363, 498, 873, 894, 929, 1041
MboI GATC 7 cut(s) 276, 361, 496, 871, 892, 927, 1039
MboII GAAGA 3 cut(s) 254, 722, 997
MfeI CAATTG 2 cut(s) 615, 1098
MhlI GDGCHC 1 cut(s) 1066
MlsI TGGCCA 1 cut(s) 333
MluCI AATT 5 cut(s) 254, 615, 819, 1046, 1098
MluNI TGGCCA 1 cut(s) 333
Mly113I GGCGCC 1 cut(s) 236
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 1 cut(s) 664
MnlI CCTC 8 cut(s) 79, 202, 302, 758, 807, 823, 950, 1128
Mox20I TGGCCA 1 cut(s) 333
MscI TGGCCA 1 cut(s) 333
MseI TTAA 4 cut(s) 390, 426, 444, 735
Msp20I TGGCCA 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 1160
MspI CCGG 3 cut(s) 564, 597, 1053
MspR9I CCNGG 3 cut(s) 519, 565, 666
MunI CAATTG 2 cut(s) 615, 1098
MvaI CCWGG 2 cut(s) 519, 666
MvnI CGCG 3 cut(s) 50, 554, 556
MwoI GCNNNNNNNGC 5 cut(s) 10, 1002, 1109, 1136, 1157
NarI GGCGCC 1 cut(s) 236
NciI CCSGG 1 cut(s) 565
NcoI CCATGG 2 cut(s) 670, 887
NdeII GATC 7 cut(s) 276, 361, 496, 871, 892, 927, 1039
NheI GCTAGC 1 cut(s) 803
NlaIII CATG 6 cut(s) 503, 674, 786, 844, 891, 1014
NlaIV GGNNCC 7 cut(s) 201, 237, 322, 385, 401, 595, 628
NmuCI GTSAC 4 cut(s) 29, 40, 502, 508
PagI TCATGA 1 cut(s) 840
PauI GCGCGC 1 cut(s) 554
PfeI GAWTC 3 cut(s) 71, 726, 774
PflMI CCANNNNNTGG 1 cut(s) 671
PleI GAGTC 1 cut(s) 239
PluTI GGCGCC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PpuMI RGGWCCY 1 cut(s) 188
PshBI ATTAAT 1 cut(s) 390
Psp1406I AACGTT 1 cut(s) 573
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 517, 664
PspGI CCWGG 2 cut(s) 517, 664
PspN4I GGNNCC 7 cut(s) 201, 237, 322, 385, 401, 595, 628
PspPI GGNCC 4 cut(s) 188, 217, 662, 1154
PspPPI RGGWCCY 1 cut(s) 188
PteI GCGCGC 1 cut(s) 554
PvuII CAGCTG 1 cut(s) 1160
RsaI GTAC 5 cut(s) 628, 701, 745, 1081, 1146
RsaNI GTAC 5 cut(s) 627, 700, 744, 1080, 1145
SaqAI TTAA 4 cut(s) 390, 426, 444, 735
Sau3AI GATC 7 cut(s) 276, 361, 496, 871, 892, 927, 1039
Sau96I GGNCC 4 cut(s) 188, 217, 662, 1154
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 3 cut(s) 519, 565, 666
SduI GDGCHC 1 cut(s) 1066
SfaNI GCATC 2 cut(s) 128, 1126
SfoI GGCGCC 1 cut(s) 237
SinI GGWCC 4 cut(s) 188, 217, 662, 1154
SmlI CTYRAG 2 cut(s) 247, 798
SmoI CTYRAG 2 cut(s) 247, 798
Sse9I AATT 5 cut(s) 254, 615, 819, 1046, 1098
SspDI GGCGCC 1 cut(s) 235
SspMI CTAG 2 cut(s) 17, 804
StyD4I CCNGG 3 cut(s) 517, 563, 664
StyI CCWWGG 5 cut(s) 220, 334, 670, 887, 999
TaaI ACNGT 2 cut(s) 508, 743
TaiI ACGT 5 cut(s) 42, 471, 494, 576, 1073
TaqI TCGA 4 cut(s) 260, 279, 495, 917
TasI AATT 5 cut(s) 254, 615, 819, 1046, 1098
TatI WGTACW 1 cut(s) 1144
TfiI GAWTC 3 cut(s) 71, 726, 774
Tru1I TTAA 4 cut(s) 390, 426, 444, 735
Tru9I TTAA 4 cut(s) 390, 426, 444, 735
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 4 cut(s) 29, 40, 502, 508
Tsp45I GTSAC 4 cut(s) 29, 40, 502, 508
TspDTI ATGAA 2 cut(s) 857, 903
TspGWI ACGGA 1 cut(s) 963
TspRI CASTG 1 cut(s) 517
Van91I CCANNNNNTGG 1 cut(s) 671
VneI GTGCAC 1 cut(s) 1062
VpaK11BI GGWCC 4 cut(s) 188, 217, 662, 1154
VspI ATTAAT 1 cut(s) 390
XagI CCTNNNNNAGG 1 cut(s) 720
XapI RAATTY 2 cut(s) 819, 1046
XspI CTAG 2 cut(s) 17, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.