Rroxscaffold_7G00214720

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000434
Physical Location & Seq
Forward (+)
65341455 .. 65343017
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214720.1

Sequence Viewer

Length: 1329 bp
ATGGATCAGAAATTAATGAACGTCTTGACAATATTTTTGTTCTTAATGTTGGCGTTTACAACTAAAATTCAATCCTCTGTCGTCTTTGACGTGACAAGTGCAAAATATGGGGGAAAGCCAAACTCAGACATTACTAAGGCTTTGCTGAAAGCTTGGACTGATGCTTGTGCATCACCGAGGGAGAGTAAAGTTCTTGTTCCAAGAGGCACCTACGGATTAAAAGGAGCAATTTTCAGAGGTCATTGCAAGGCTCCTATTGAACTTCAGGTCCGAGGCACATTGCAGGCTCCAAAAGACACTAGCCAAGTCACGTCACCGGATACTTGGGTTGGTTTTCGGTACCTTAATAGGCTCACCTTGTCAGGTGGTGGAACTTTTGATGGTCGCGGAGCACTCTCTTGGAAACAGAACGACTGTAACGAAAACAAAAACTGCAAATCTCGGGTCGTGAACATAAGGTTCGACTTCGTCAACGATACAATAATTAAGGACATAACTTCACTTGACAGCAAGAACTTTCACTTCAACGTTTTCGCCTGCCACAATGTTACTTTCCAACATGCAACCATCACAGCACCCGAAGAGAGTGTTAACACAGACGGCCATGGCATAAGCATTGGAAGCCTCGGAGGGCATCGCGATGAACAACCTGTAACTGGCGTCATAATCAAGAACGTAACATTCAATAATACCCAGAATGGTGCAAGAATCAAGACGTGGCCAGCCTCTTATGCCGGCCTTGTGTCAGATATACACTATGAGGATATTACCATGGTTAATGTCAGCAACCCGATCATTATAGACCAGGAGTACTGCCCATGGAATTTGTGCAACAAGCAGATTCCTTCCAAAGTTAAGATCAGTGATGTGAGCTTCAAGAACATTAAAGGCACGTCCACAACTCCGCTTGCGGTAAATTTTATTTGTAGCAGGAGCGTGCCATGCGAGAATGTGGAGATTTCCGGCATTGATCTCATTTACACTGGACAAAAAGGACTTATTAATTCACAATGCTCCAATGTCAAACTGGCGACGATTGTTAACGTGACAAAGGCTCTTGCTTGTGGTAACCATCCACCAATAATTACATCAAAGAACCACTCCAAGAGGCATACTTCAACAATAATTAGTCGATCAAAGAATTACCAGACCGAGACGAAAAACCAGATTGATTCTGTTGTAGGGAATGAGCCAACCAGAGGTCATCATCGTAAGTATGGTCTGATTGCGACGTCCTCGGCCCCAGAAAGTGATGATTACTTTTCAACAGTGTCGTCCTCCGCTCCAAAGAGTCGCAGTACTTATGGGTGGCTACAACCGATGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

442

Amino Acids

48.56

Weight (kDa)

9.17

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 201 6.3e-28 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 201 - 342 3.1e-45 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1235
Acc65I GGTACC 1 cut(s) 339
AccB1I GGYRCC 2 cut(s) 206, 339
AccBSI CCGCTC 1 cut(s) 1283
AccII CGCG 2 cut(s) 387, 639
AciI CCGC 4 cut(s) 387, 905, 911, 1281
AclI AACGTT 1 cut(s) 528
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 601, 719
AcsI RAATTY 3 cut(s) 66, 823, 916
AcuI CTGAAG 1 cut(s) 248
AcyI GRCGYC 2 cut(s) 660, 1232
AfaI GTAC 3 cut(s) 341, 812, 1300
AfiI CCNNNNNNNGG 2 cut(s) 656, 1199
AgsI TTSAA 7 cut(s) 71, 260, 526, 685, 877, 1119, 1266
AjiI CACGTC 4 cut(s) 91, 312, 717, 894
AjnI CCWGG 1 cut(s) 804
AloI GAACNNNNNNTCC 2 cut(s) 252, 284
AluBI AGCT 2 cut(s) 152, 873
AluI AGCT 2 cut(s) 152, 873
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 1 cut(s) 1148
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 441
AoxI GGCC 4 cut(s) 601, 719, 736, 1239
ApoI RAATTY 3 cut(s) 66, 823, 916
ArsI GACNNNNNNTTYG 4 cut(s) 19, 51, 429, 461
AseI ATTAAT 2 cut(s) 14, 1002
Asp718I GGTACC 1 cut(s) 339
AspS9I GGNCC 2 cut(s) 268, 1240
AsuHPI GGTGA 3 cut(s) 165, 306, 346
AvaI CYCGRG 1 cut(s) 441
AvaII GGWCC 1 cut(s) 268
BaeI ACNNNNGTAYC 2 cut(s) 312, 345
BalI TGGCCA 1 cut(s) 721
BanI GGYRCC 2 cut(s) 206, 339
Bbv12I GWGCWC 1 cut(s) 394
BccI CCATC 3 cut(s) 374, 575, 1080
BceAI ACGGC 1 cut(s) 616
BciT130I CCWGG 1 cut(s) 806
BciVI GTATCC 1 cut(s) 313
BcoDI GTCTC 1 cut(s) 1148
BfaI CTAG 1 cut(s) 300
BfuI GTATCC 1 cut(s) 313
BmcAI AGTACT 2 cut(s) 812, 1300
Bme1390I CCNGG 1 cut(s) 806
Bme18I GGWCC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 441
BmgBI CACGTC 4 cut(s) 91, 312, 717, 894
BmgT120I GGNCC 2 cut(s) 268, 1240
BmiI GGNNCC 5 cut(s) 208, 252, 288, 341, 1242
BmrFI CCNGG 1 cut(s) 806
BmsI GCATC 4 cut(s) 151, 179, 643, 1311
BsaHI GRCGYC 2 cut(s) 660, 1232
BsaJI CCNNGG 7 cut(s) 176, 271, 604, 625, 771, 818, 1236
BsaWI WCCGGW 1 cut(s) 316
Bsc4I CCNNNNNNNGG 2 cut(s) 656, 1199
Bse118I RCCGGY 1 cut(s) 734
Bse1I ACTGG 3 cut(s) 661, 988, 1032
Bse3DI GCAATG 2 cut(s) 241, 278
BseBI CCWGG 1 cut(s) 806
BseDI CCNNGG 7 cut(s) 176, 271, 604, 625, 771, 818, 1236
BseGI GGATG 1 cut(s) 1072
BseLI CCNNNNNNNGG 2 cut(s) 656, 1199
BseMI GCAATG 2 cut(s) 241, 278
BseMII CTCAG 1 cut(s) 138
BseNI ACTGG 3 cut(s) 661, 988, 1032
Bsh1236I CGCG 2 cut(s) 387, 639
BshFI GGCC 4 cut(s) 603, 721, 738, 1241
BshNI GGYRCC 2 cut(s) 206, 339
BsiHKAI GWGCWC 1 cut(s) 394
BsiHKCI CYCGRG 1 cut(s) 441
BsiSI CCGG 3 cut(s) 317, 735, 963
BslI CCNNNNNNNGG 2 cut(s) 656, 1199
BsmAI GTCTC 1 cut(s) 1148
BsmBI CGTCTC 1 cut(s) 1148
BsnI GGCC 4 cut(s) 603, 721, 738, 1241
BsoBI CYCGRG 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 5 cut(s) 4, 792, 858, 970, 1133
Bsp19I CCATGG 3 cut(s) 604, 771, 818
Bsp68I TCGCGA 1 cut(s) 639
BspACI CCGC 4 cut(s) 387, 905, 911, 1281
BspANI GGCC 4 cut(s) 603, 721, 738, 1241
BspCNI CTCAG 1 cut(s) 137
BspFNI CGCG 2 cut(s) 387, 639
BspLI GGNNCC 5 cut(s) 208, 252, 288, 341, 1242
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 206, 339
BsrBI CCGCTC 1 cut(s) 1283
BsrDI GCAATG 2 cut(s) 241, 278
BsrFI RCCGGY 1 cut(s) 734
BsrI ACTGG 3 cut(s) 661, 988, 1032
BssAI RCCGGY 1 cut(s) 734
BssECI CCNNGG 7 cut(s) 176, 271, 604, 625, 771, 818, 1236
BssMI GATC 5 cut(s) 4, 792, 858, 970, 1133
BssNI GRCGYC 2 cut(s) 660, 1232
BssT1I CCWWGG 3 cut(s) 604, 771, 818
Bst2UI CCWGG 1 cut(s) 806
Bst4CI ACNGT 2 cut(s) 416, 1270
Bst6I CTCTTC 1 cut(s) 576
BstACI GRCGYC 2 cut(s) 660, 1232
BstC8I GCNNGC 6 cut(s) 285, 538, 723, 736, 909, 938
BstDEI CTNAG 2 cut(s) 124, 135
BstDSI CCRYGG 3 cut(s) 604, 771, 818
BstEII GGTNACC 1 cut(s) 1067
BstF5I GGATG 1 cut(s) 1072
BstFNI CGCG 2 cut(s) 387, 639
BstKTI GATC 5 cut(s) 7, 795, 861, 973, 1136
BstMAI GTCTC 1 cut(s) 1148
BstMBI GATC 5 cut(s) 4, 792, 858, 970, 1133
BstMWI GCNNNNNNNGC 3 cut(s) 621, 731, 942
BstNI CCWGG 1 cut(s) 806
BstNSI RCATGY 1 cut(s) 563
BstPI GGTNACC 1 cut(s) 1067
BstSCI CCNGG 1 cut(s) 804
BstUI CGCG 2 cut(s) 387, 639
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 4 cut(s) 603, 721, 738, 1241
BtgI CCRYGG 3 cut(s) 604, 771, 818
BtgZI GCGATG 2 cut(s) 620, 654
BtrI CACGTC 4 cut(s) 91, 312, 717, 894
BtsCI GGATG 1 cut(s) 1072
BtsIMutI CAGTG 3 cut(s) 868, 981, 1275
BtuMI TCGCGA 1 cut(s) 639
Cac8I GCNNGC 6 cut(s) 285, 538, 723, 736, 909, 938
Cfr10I RCCGGY 1 cut(s) 734
Cfr13I GGNCC 2 cut(s) 268, 1240
CseI GACGC 1 cut(s) 649
Csp6I GTAC 3 cut(s) 340, 811, 1299
CviAII CATG 5 cut(s) 560, 605, 772, 819, 942
CviQI GTAC 3 cut(s) 340, 811, 1299
DdeI CTNAG 2 cut(s) 124, 135
DpnI GATC 5 cut(s) 6, 794, 860, 972, 1135
DpnII GATC 5 cut(s) 4, 792, 858, 970, 1133
EaeI YGGCCR 2 cut(s) 601, 719
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Eco130I CCWWGG 3 cut(s) 604, 771, 818
Eco47I GGWCC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 248
Eco88I CYCGRG 1 cut(s) 441
Eco91I GGTNACC 1 cut(s) 1067
EcoO65I GGTNACC 1 cut(s) 1067
EcoRII CCWGG 1 cut(s) 804
EcoT14I CCWWGG 3 cut(s) 604, 771, 818
ErhI CCWWGG 3 cut(s) 604, 771, 818
Esp3I CGTCTC 1 cut(s) 1148
FaeI CATG 5 cut(s) 563, 608, 775, 822, 945
FatI CATG 5 cut(s) 559, 604, 771, 818, 941
FokI GGATG 1 cut(s) 1059
FspBI CTAG 1 cut(s) 300
HaeIII GGCC 4 cut(s) 603, 721, 738, 1241
HapII CCGG 3 cut(s) 317, 735, 963
HgaI GACGC 1 cut(s) 649
Hin1I GRCGYC 2 cut(s) 660, 1232
Hin1II CATG 5 cut(s) 563, 608, 775, 822, 945
HincII GTYRAC 3 cut(s) 472, 592, 1042
HindII GTYRAC 3 cut(s) 472, 592, 1042
HindIII AAGCTT 1 cut(s) 150
HinfI GANTC 4 cut(s) 708, 841, 1172, 1291
HpaI GTTAAC 2 cut(s) 592, 1042
HpaII CCGG 3 cut(s) 317, 735, 963
HphI GGTGA 3 cut(s) 165, 306, 346
Hpy166II GTNNAC 6 cut(s) 57, 451, 472, 592, 897, 1042
Hpy188I TCNGA 7 cut(s) 9, 127, 236, 272, 629, 748, 1224
Hpy188III TCNNGA 6 cut(s) 25, 448, 638, 670, 712, 877
Hpy8I GTNNAC 6 cut(s) 57, 451, 472, 592, 897, 1042
Hpy99I CGWCG 2 cut(s) 1036, 1234
HpyAV CCTTC 1 cut(s) 855
HpyCH4III ACNGT 2 cut(s) 416, 1270
HpyCH4IV ACGT 9 cut(s) 21, 90, 311, 528, 675, 716, 893, 1044, 1232
HpyCH4V TGCA 8 cut(s) 101, 170, 246, 283, 435, 563, 704, 831
HpyF10VI GCNNNNNNNGC 3 cut(s) 621, 731, 942
HpyF3I CTNAG 2 cut(s) 124, 135
HpySE526I ACGT 9 cut(s) 21, 90, 311, 528, 675, 716, 893, 1044, 1232
Hsp92I GRCGYC 2 cut(s) 660, 1232
Hsp92II CATG 5 cut(s) 563, 608, 775, 822, 945
KpnI GGTACC 1 cut(s) 343
KroI GCCGGC 1 cut(s) 734
KroNI GCCGGC 1 cut(s) 736
KspAI GTTAAC 2 cut(s) 592, 1042
Kzo9I GATC 5 cut(s) 4, 792, 858, 970, 1133
LmnI GCTCC 7 cut(s) 224, 256, 292, 389, 933, 1019, 1288
LweI GCATC 4 cut(s) 151, 179, 643, 1311
MaeI CTAG 1 cut(s) 300
MaeII ACGT 9 cut(s) 21, 90, 311, 528, 675, 716, 893, 1044, 1232
MaeIII GTNAC 9 cut(s) 91, 307, 312, 416, 547, 652, 676, 1045, 1067
MalI GATC 5 cut(s) 6, 794, 860, 972, 1135
MbiI CCGCTC 1 cut(s) 1283
MboI GATC 5 cut(s) 4, 792, 858, 970, 1133
MboII GAAGA 1 cut(s) 593
MhlI GDGCHC 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 721
MluNI TGGCCA 1 cut(s) 721
MlyI GAGTC 1 cut(s) 1300
MmeI TCCRAC 1 cut(s) 580
Mox20I TGGCCA 1 cut(s) 721
MroNI GCCGGC 1 cut(s) 734
MscI TGGCCA 1 cut(s) 721
MslI CAYNNNNRTG 1 cut(s) 639
Msp20I TGGCCA 1 cut(s) 721
MspI CCGG 3 cut(s) 317, 735, 963
MspR9I CCNGG 1 cut(s) 806
MvaI CCWGG 1 cut(s) 806
MvnI CGCG 2 cut(s) 387, 639
MwoI GCNNNNNNNGC 3 cut(s) 621, 731, 942
NaeI GCCGGC 1 cut(s) 736
NcoI CCATGG 3 cut(s) 604, 771, 818
NdeII GATC 5 cut(s) 4, 792, 858, 970, 1133
NgoMIV GCCGGC 1 cut(s) 734
NlaIII CATG 5 cut(s) 563, 608, 775, 822, 945
NlaIV GGNNCC 5 cut(s) 208, 252, 288, 341, 1242
NmeAIII GCCGAG 1 cut(s) 1217
NmuCI GTSAC 4 cut(s) 91, 307, 312, 1045
NruI TCGCGA 1 cut(s) 639
NspI RCATGY 1 cut(s) 563
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 417
PdiI GCCGGC 1 cut(s) 736
PfeI GAWTC 3 cut(s) 708, 841, 1172
PflFI GACNNNGTC 1 cut(s) 467
PleI GAGTC 1 cut(s) 1299
PpsI GAGTC 1 cut(s) 1299
PshBI ATTAAT 2 cut(s) 14, 1002
Psp1406I AACGTT 1 cut(s) 528
Psp6I CCWGG 1 cut(s) 804
PspEI GGTNACC 1 cut(s) 1067
PspGI CCWGG 1 cut(s) 804
PspN4I GGNNCC 5 cut(s) 208, 252, 288, 341, 1242
PspPI GGNCC 2 cut(s) 268, 1240
PsyI GACNNNGTC 1 cut(s) 467
RruI TCGCGA 1 cut(s) 639
RsaI GTAC 3 cut(s) 341, 812, 1300
RsaNI GTAC 3 cut(s) 340, 811, 1299
RseI CAYNNNNRTG 1 cut(s) 639
Sau3AI GATC 5 cut(s) 4, 792, 858, 970, 1133
Sau96I GGNCC 2 cut(s) 268, 1240
ScaI AGTACT 2 cut(s) 812, 1300
SchI GAGTC 1 cut(s) 1300
ScrFI CCNGG 1 cut(s) 806
SduI GDGCHC 1 cut(s) 394
SfaNI GCATC 4 cut(s) 151, 179, 643, 1311
SinI GGWCC 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 639
SsiI CCGC 4 cut(s) 387, 905, 911, 1281
SspI AATATT 1 cut(s) 33
SspMI CTAG 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 804
StyI CCWWGG 3 cut(s) 604, 771, 818
TaaI ACNGT 2 cut(s) 416, 1270
TaiI ACGT 9 cut(s) 24, 93, 314, 531, 678, 719, 896, 1047, 1235
TaqI TCGA 2 cut(s) 462, 1132
TaqII GACCGA 1 cut(s) 1166
TatI WGTACW 2 cut(s) 810, 1298
TfiI GAWTC 3 cut(s) 708, 841, 1172
TscAI CASTG 3 cut(s) 868, 988, 1275
TseFI GTSAC 4 cut(s) 91, 307, 312, 1045
Tsp45I GTSAC 4 cut(s) 91, 307, 312, 1045
TspDTI ATGAA 2 cut(s) 32, 657
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 3 cut(s) 868, 988, 1275
Tth111I GACNNNGTC 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 268
VspI ATTAAT 2 cut(s) 14, 1002
XapI RAATTY 3 cut(s) 66, 823, 916
XceI RCATGY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 1024
XspI CTAG 1 cut(s) 300
ZraI GACGTC 1 cut(s) 1233
ZrmI AGTACT 2 cut(s) 812, 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.