Rh2BG553200

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000434
Physical Location & Seq
Forward (+)
76131783 .. 76132895
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG553200.1

Sequence Viewer

Length: 1113 bp
ATGGGTCTCAAATTGATGACTATCATGGCGATATTCATGTTCTTATTGTTAGCAGCTACAGCTAAAGCCCAACCCTGTGTAGTCTTTGACGTGACGAGTGCAAAATATGGTGGAAAAACTAACTATGATATTACAGACTCCTCAGCACAAGCTTGGACAGATGCTTGTGCTTCTGTATGGTCGAGTAAACTCGTTGTTCCAAGCGGCACATATTGGTTAAGAGGAGCAACTTTTATAGGTCCTTGCAAGGCTCCTATCAAGGTGCAAATCCAAGGAAAGTTGAAAGATCCCGAAGATGGCCACCAACTCGTGAACTCGGATTTTTGGGTTGCTTTTCGGTACATTGACAGGCTCTACTTATCAGGTGGTGGAACTTTTGATGGCCAAGGAGCCCTTGGTTGGAATACTTATCGTGCTATTAACGTGAGGTTCGACTACATCTCCAACTCCATAATTCAGGACATAACTTCCCTTAACAGCAGAAGTTTCCATATTAACGTTTGTGCATGCCATGATGTTACATTCCAGGGTGTAACTGTCGTAGCACCCGGCGAGAGTTCTAATACAGATGGAATGCACATTGCACATTCGTGGAGGATCAACGTAACGGATACAAACATCGAAACTGGAGATGATTGTATTTCTATTTGTAATGGCACTTCGCAACTCACAGTAACCAATGTTAATTGTGGACCGGGACATGGAATAAGCATAGGAAGTCTTGGAAAGTATCTTGATGAAAAACCGGTATATGGCCTCATCATTAAGAACTGCACCCTCACGAACACAACAAACGGTGTGAGAATCAAGACATGGCCTAGTTCTCCCAGTGCTCGTTTTGCCTTACACATATACTTTGAGGATATTATCATGAATAATGTGAAGAACCCCATCATTATAGACCAAAACTACTGCCCCAGGCATTGTTGCAAACAACAGGCTCAGTTGAACATTAAGATTAGTAATGTCAGCTTCAAGAACATTAGAGGCTCATCTGCATCACAAGTTGCCGTCAATATTGCATGTAGTGGGAGCTTACCATGTGAGAATGTAGAATTGATAGGCATTGATCTAGTATACAGTGGAGATGGCAATCGACCTATTACATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

370

Amino Acids

40.25

Weight (kDa)

7.48

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 363 5.6e-83 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1077
AciI CCGC 1 cut(s) 204
AclI AACGTT 1 cut(s) 498
AclWI GGATC 2 cut(s) 281, 605
AcoI YGGCCR 2 cut(s) 298, 382
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 4 cut(s) 296, 399, 701, 752
AgeI ACCGGT 1 cut(s) 745
AgsI TTSAA 3 cut(s) 283, 949, 976
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 2 cut(s) 525, 917
AleI CACNNNNGTG 1 cut(s) 589
AluBI AGCT 5 cut(s) 56, 62, 152, 972, 1035
AluI AGCT 5 cut(s) 56, 62, 152, 972, 1035
Alw21I GWGCWC 1 cut(s) 835
Alw26I GTCTC 1 cut(s) 11
AlwI GGATC 2 cut(s) 281, 605
AoxI GGCC 4 cut(s) 298, 382, 754, 815
ApeKI GCWGC 1 cut(s) 53
AsiGI ACCGGT 1 cut(s) 745
AspS9I GGNCC 2 cut(s) 239, 692
AsuC2I CCSGG 2 cut(s) 549, 696
AvaII GGWCC 2 cut(s) 239, 692
BalI TGGCCA 2 cut(s) 300, 384
BanII GRGCYC 1 cut(s) 394
BarI GAAGNNNNNNTAC 2 cut(s) 643, 675
BauI CACGAG 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 835
BbvCI CCTCAGC 1 cut(s) 142
BbvI GCAGC 1 cut(s) 65
BccI CCATC 5 cut(s) 290, 374, 563, 899, 1082
BceAI ACGGC 1 cut(s) 995
BciT130I CCWGG 2 cut(s) 527, 919
BciVI GTATCC 1 cut(s) 604
BcnI CCSGG 2 cut(s) 549, 696
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 2 cut(s) 819, 1073
BfmI CTRYAG 1 cut(s) 57
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 2 cut(s) 54, 205
BlsI GCNGC 2 cut(s) 55, 206
Bme1390I CCNGG 4 cut(s) 527, 549, 696, 919
Bme18I GGWCC 2 cut(s) 239, 692
BmgBI CACGTC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 239, 692
BmiI GGNNCC 2 cut(s) 252, 391
BmrFI CCNGG 4 cut(s) 527, 549, 696, 919
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 2 cut(s) 151, 1007
BmuI ACTGGG 1 cut(s) 822
BpmI CTGGAG 1 cut(s) 648
Bpu10I CCTNAGC 1 cut(s) 142
BpuMI CCSGG 2 cut(s) 549, 696
BsaBI GATNNNNATC 2 cut(s) 20, 1092
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 5 cut(s) 271, 385, 394, 526, 917
BsaWI WCCGGW 1 cut(s) 745
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 399, 701, 752
Bse118I RCCGGY 1 cut(s) 745
Bse1I ACTGG 2 cut(s) 631, 828
Bse3DI GCAATG 1 cut(s) 579
Bse8I GATNNNNATC 2 cut(s) 20, 1092
BseBI CCWGG 2 cut(s) 527, 919
BseDI CCNNGG 5 cut(s) 271, 385, 394, 526, 917
BseJI GATNNNNATC 2 cut(s) 20, 1092
BseLI CCNNNNNNNGG 4 cut(s) 296, 399, 701, 752
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 2 cut(s) 156, 956
BseNI ACTGG 2 cut(s) 631, 828
BseRI GAGGAG 2 cut(s) 130, 237
BseXI GCAGC 1 cut(s) 65
BsgI GTGCAG 1 cut(s) 757
BshFI GGCC 4 cut(s) 300, 384, 756, 817
BshTI ACCGGT 1 cut(s) 745
BsiHKAI GWGCWC 1 cut(s) 835
BsiSI CCGG 3 cut(s) 549, 695, 746
BslFI GGGAC 1 cut(s) 711
BslI CCNNNNNNNGG 4 cut(s) 296, 399, 701, 752
BsmAI GTCTC 1 cut(s) 11
BsmFI GGGAC 1 cut(s) 711
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 4 cut(s) 300, 384, 756, 817
Bso31I GGTCTC 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 394, 835
Bsp143I GATC 3 cut(s) 286, 597, 1069
BspACI CCGC 1 cut(s) 204
BspANI GGCC 4 cut(s) 300, 384, 756, 817
BspCNI CTCAG 2 cut(s) 155, 955
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 2 cut(s) 252, 391
BspPI GGATC 2 cut(s) 281, 605
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 579
BsrFI RCCGGY 1 cut(s) 745
BsrI ACTGG 2 cut(s) 631, 828
BssAI RCCGGY 1 cut(s) 745
BssECI CCNNGG 5 cut(s) 271, 385, 394, 526, 917
BssMI GATC 3 cut(s) 286, 597, 1069
BssNAI GTATAC 1 cut(s) 1078
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 3 cut(s) 271, 385, 394
Bst1107I GTATAC 1 cut(s) 1078
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 2 cut(s) 527, 919
Bst4CI ACNGT 4 cut(s) 538, 673, 797, 1082
BstC8I GCNNGC 1 cut(s) 508
BstDEI CTNAG 2 cut(s) 142, 942
BstKTI GATC 3 cut(s) 289, 600, 1072
BstMAI GTCTC 1 cut(s) 11
BstMBI GATC 3 cut(s) 286, 597, 1069
BstMWI GCNNNNNNNGC 2 cut(s) 59, 839
BstNI CCWGG 2 cut(s) 527, 919
BstNSI RCATGY 2 cut(s) 510, 1026
BstSCI CCNGG 4 cut(s) 525, 547, 694, 917
BstSFI CTRYAG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 65
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
BstZ17I GTATAC 1 cut(s) 1078
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 4 cut(s) 300, 384, 756, 817
BtrI CACGTC 1 cut(s) 91
BtsIMutI CAGTG 2 cut(s) 835, 1087
Cac8I GCNNGC 1 cut(s) 508
CciI TCATGA 1 cut(s) 870
Cfr10I RCCGGY 1 cut(s) 745
Cfr13I GGNCC 2 cut(s) 239, 692
Csp6I GTAC 1 cut(s) 340
CspAI ACCGGT 1 cut(s) 745
CviAII CATG 9 cut(s) 25, 37, 507, 512, 701, 813, 871, 1023, 1041
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 2 cut(s) 142, 942
DpnI GATC 3 cut(s) 288, 599, 1071
DpnII GATC 3 cut(s) 286, 597, 1069
EaeI YGGCCR 2 cut(s) 298, 382
Eco130I CCWWGG 3 cut(s) 271, 385, 394
Eco24I GRGCYC 1 cut(s) 394
Eco31I GGTCTC 1 cut(s) 11
Eco47I GGWCC 2 cut(s) 239, 692
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 2 cut(s) 525, 917
EcoT14I CCWWGG 3 cut(s) 271, 385, 394
EcoT38I GRGCYC 1 cut(s) 394
ErhI CCWWGG 3 cut(s) 271, 385, 394
FaeI CATG 9 cut(s) 28, 40, 510, 515, 704, 816, 874, 1026, 1044
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 1 cut(s) 711
FatI CATG 9 cut(s) 24, 36, 506, 511, 700, 812, 870, 1022, 1040
FblI GTMKAC 1 cut(s) 1077
Fnu4HI GCNGC 2 cut(s) 54, 205
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 2 cut(s) 54, 205
FspBI CTAG 2 cut(s) 819, 1073
GluI GCNGC 2 cut(s) 54, 205
GsuI CTGGAG 1 cut(s) 648
HaeIII GGCC 4 cut(s) 300, 384, 756, 817
HapII CCGG 3 cut(s) 549, 695, 746
Hin1II CATG 9 cut(s) 28, 40, 510, 515, 704, 816, 874, 1026, 1044
HindIII AAGCTT 1 cut(s) 150
HinfI GANTC 2 cut(s) 137, 804
HpaII CCGG 3 cut(s) 549, 695, 746
Hpy166II GTNNAC 4 cut(s) 188, 313, 692, 1078
Hpy188I TCNGA 1 cut(s) 319
Hpy188III TCNNGA 9 cut(s) 290, 310, 458, 734, 781, 808, 871, 976, 1110
Hpy8I GTNNAC 4 cut(s) 188, 313, 692, 1078
HpyCH4III ACNGT 4 cut(s) 538, 673, 797, 1082
HpyCH4IV ACGT 4 cut(s) 90, 423, 498, 603
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 839
HpyF3I CTNAG 2 cut(s) 142, 942
HpySE526I ACGT 4 cut(s) 90, 423, 498, 603
Hsp92II CATG 9 cut(s) 28, 40, 510, 515, 704, 816, 874, 1026, 1044
Kzo9I GATC 3 cut(s) 286, 597, 1069
LmnI GCTCC 4 cut(s) 224, 256, 389, 1032
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 2 cut(s) 151, 1007
MaeI CTAG 2 cut(s) 819, 1073
MaeII ACGT 4 cut(s) 90, 423, 498, 603
MaeIII GTNAC 5 cut(s) 91, 517, 532, 604, 673
MalI GATC 3 cut(s) 288, 599, 1071
MboI GATC 3 cut(s) 286, 597, 1069
MboII GAAGA 2 cut(s) 305, 895
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 2 cut(s) 394, 835
MlsI TGGCCA 2 cut(s) 300, 384
MluCI AATT 4 cut(s) 11, 453, 685, 1055
MluNI TGGCCA 2 cut(s) 300, 384
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 380, 468
MnlI CCTC 8 cut(s) 151, 215, 420, 588, 767, 788, 853, 980
Mox20I TGGCCA 2 cut(s) 300, 384
MscI TGGCCA 2 cut(s) 300, 384
MseI TTAA 7 cut(s) 218, 420, 474, 495, 684, 765, 954
MslI CAYNNNNRTG 1 cut(s) 589
Msp20I TGGCCA 2 cut(s) 300, 384
MspI CCGG 3 cut(s) 549, 695, 746
MspR9I CCNGG 4 cut(s) 527, 549, 696, 919
Mva1269I GAATGC 1 cut(s) 579
MvaI CCWGG 2 cut(s) 527, 919
MwoI GCNNNNNNNGC 2 cut(s) 59, 839
NciI CCSGG 2 cut(s) 549, 696
NdeII GATC 3 cut(s) 286, 597, 1069
NlaIII CATG 9 cut(s) 28, 40, 510, 515, 704, 816, 874, 1026, 1044
NlaIV GGNNCC 2 cut(s) 252, 391
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 2 cut(s) 510, 1026
OliI CACNNNNGTG 1 cut(s) 589
PaeI GCATGC 1 cut(s) 510
PagI TCATGA 1 cut(s) 870
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PctI GAATGC 1 cut(s) 579
PfeI GAWTC 1 cut(s) 804
PinAI ACCGGT 1 cut(s) 745
PkrI GCNGC 2 cut(s) 55, 206
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PpuMI RGGWCCY 1 cut(s) 239
Psp1406I AACGTT 1 cut(s) 498
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 525, 917
PspGI CCWGG 2 cut(s) 525, 917
PspN4I GGNNCC 2 cut(s) 252, 391
PspPI GGNCC 2 cut(s) 239, 692
PspPPI RGGWCCY 1 cut(s) 239
PsuI RGATCY 1 cut(s) 286
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 1 cut(s) 589
SaqAI TTAA 7 cut(s) 218, 420, 474, 495, 684, 765, 954
SatI GCNGC 2 cut(s) 54, 205
Sau3AI GATC 3 cut(s) 286, 597, 1069
Sau96I GGNCC 2 cut(s) 239, 692
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 4 cut(s) 527, 549, 696, 919
SduI GDGCHC 2 cut(s) 394, 835
SfaNI GCATC 2 cut(s) 151, 1007
SfcI CTRYAG 1 cut(s) 57
SinI GGWCC 2 cut(s) 239, 692
SmiMI CAYNNNNRTG 1 cut(s) 589
SphI GCATGC 1 cut(s) 510
Sse9I AATT 4 cut(s) 11, 453, 685, 1055
SsiI CCGC 1 cut(s) 204
SspI AATATT 1 cut(s) 1018
SspMI CTAG 2 cut(s) 819, 1073
StyD4I CCNGG 4 cut(s) 525, 547, 694, 917
StyI CCWWGG 3 cut(s) 271, 385, 394
TaaI ACNGT 4 cut(s) 538, 673, 797, 1082
TaiI ACGT 4 cut(s) 93, 426, 501, 606
TaqI TCGA 4 cut(s) 182, 432, 621, 1096
TasI AATT 4 cut(s) 11, 453, 685, 1055
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 1 cut(s) 804
Tru1I TTAA 7 cut(s) 218, 420, 474, 495, 684, 765, 954
Tru9I TTAA 7 cut(s) 218, 420, 474, 495, 684, 765, 954
TscAI CASTG 2 cut(s) 835, 1087
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 3 cut(s) 25, 753, 887
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 2 cut(s) 835, 1087
VpaK11BI GGWCC 2 cut(s) 239, 692
XceI RCATGY 2 cut(s) 510, 1026
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 1077
XspI CTAG 2 cut(s) 819, 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.