MD00G1117900.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000434
Physical Location & Seq
Reverse (-)
25042938 .. 25044428
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1117900.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGAGGGGACTTGGGTCGGTTTCAAGCACGTTGACATGATCACCGTATCAGGTGGCGAATCTTTTGATGGCCAAGGAGCATTTGCTTGGAGTCAAAATAACTGCAGCAAAAGCAAACATTGCTCTGGTTTCGCTAATCTGAGGTTCGACTTCGTCACAAATTCCATAATTCAGGACGTAACTTCACTTATTAGCAAAAACTTCCATGTCCATGTTTACGGGTGCAACAATGTTACATTCCAACATGTTCACGTCACAGCACCTGGAGACAACAAAAATATTGATGGAATCCATATCGGCAAATCAACAGGGATCAACATTACTCATACATATATTGGAACTGGAGATGACTGTGTTTCTATAGGAGATGGCAACAACCAAATCGCCGTGACCAACGTTACTTGTGGGCCAGGTCATGGCATAAGCATTGGAAGTCTCGGAAGATACGACAATGAGGAATCCCTGGCTGGAATCATAGTAAAAAACTACACCCTGACAAACACACAGAATGGTGTGCGCATCAAAACATGGCCGAATTCTCCTATGGCAACCGCTGCCTCAGATATACACTTTGAGGATATTATCATGGTTAATAATTCTCTCACTCATTCACGAGTTTTGTGCTTGTGTTGTCATGGGCTGGATCGTATGTCTTGGTCCAAGACAAGATTACCATTCAACAACCCTATTGTCATAGACCAACTTTACTGCCCGTATAAGAAGTGTGACAATTTGCCTCCGTCAGAAGTTAAGATCAACGATGTCATCTTCAAGAACATTAAGGGCTCATCTGCAACTGCACTTTCACTAAAGATTGTGTGCAGCAGTGGCTCACCATGTGAGAATGTTGAGTTGGCTGACGTTGTTCTCACCTACAGTGGAGCCAAAGGAACTCTTACCTCTCAATGTTCAAACGTCAAGCCAACAATTTCTGGCCTGACCAGGGCTCTTACTTGCGCTACATCCTCCGTGATGCTTCCTCCCTTGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

331

Amino Acids

35.48

Weight (kDa)

6.84

Isoelectric Point (pI)

20.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 4 - 198 1.1e-58 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 227 - 309 3.4e-18 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 518
AccB7I CCANNNNNTGG 1 cut(s) 416
AciI CCGC 1 cut(s) 552
AclI AACGTT 1 cut(s) 396
AclWI GGATC 2 cut(s) 320, 651
AcoI YGGCCR 2 cut(s) 70, 530
AcsI RAATTY 2 cut(s) 160, 535
AdeI CACNNNGTG 1 cut(s) 839
AfiI CCNNNNNNNGG 2 cut(s) 416, 943
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 4 cut(s) 25, 679, 772, 912
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 4 cut(s) 262, 409, 462, 941
AjuI GAANNNNNNNTTGG 2 cut(s) 836, 868
Alw26I GTCTC 2 cut(s) 261, 440
AlwI GGATC 2 cut(s) 320, 651
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 4 cut(s) 70, 407, 530, 934
ApeKI GCWGC 3 cut(s) 105, 554, 822
ApoI RAATTY 2 cut(s) 160, 535
AspLEI GCGC 2 cut(s) 519, 959
AspS9I GGNCC 2 cut(s) 407, 657
AsuHPI GGTGA 3 cut(s) 34, 825, 862
AvaII GGWCC 1 cut(s) 657
BalI TGGCCA 1 cut(s) 72
BanII GRGCYC 2 cut(s) 788, 949
BauI CACGAG 1 cut(s) 612
BbvI GCAGC 3 cut(s) 117, 541, 834
BccI CCATC 3 cut(s) 62, 278, 362
BceAI ACGGC 1 cut(s) 371
BcgI CGANNNNNNTGC 2 cut(s) 603, 637
BciT130I CCWGG 4 cut(s) 264, 411, 464, 943
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 2 cut(s) 261, 440
BfmI CTRYAG 3 cut(s) 103, 360, 874
BisI GCNGC 3 cut(s) 106, 555, 823
BlsI GCNGC 3 cut(s) 107, 556, 824
Bme1390I CCNGG 4 cut(s) 264, 411, 464, 943
Bme18I GGWCC 1 cut(s) 657
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 2 cut(s) 407, 657
BmiI GGNNCC 1 cut(s) 883
BmrFI CCNGG 4 cut(s) 264, 411, 464, 943
BmsI GCATC 2 cut(s) 528, 963
BoxI GACNNNNGTC 1 cut(s) 14
BpmI CTGGAG 2 cut(s) 285, 363
BsaJI CCNNGG 3 cut(s) 73, 462, 942
Bsc4I CCNNNNNNNGG 2 cut(s) 416, 943
Bse1I ACTGG 1 cut(s) 346
Bse3DI GCAATG 1 cut(s) 118
BseBI CCWGG 4 cut(s) 264, 411, 464, 943
BseDI CCNNGG 3 cut(s) 73, 462, 942
BseGI GGATG 1 cut(s) 962
BseLI CCNNNNNNNGG 2 cut(s) 416, 943
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 2 cut(s) 131, 573
BseNI ACTGG 1 cut(s) 346
BseXI GCAGC 3 cut(s) 117, 541, 834
BsgI GTGCAG 2 cut(s) 783, 841
BshFI GGCC 4 cut(s) 72, 409, 532, 936
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 416, 943
BsmAI GTCTC 2 cut(s) 261, 440
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 4 cut(s) 72, 409, 532, 936
Bsp1286I GDGCHC 2 cut(s) 788, 949
Bsp143I GATC 5 cut(s) 39, 312, 643, 753, 987
BspACI CCGC 1 cut(s) 552
BspANI GGCC 4 cut(s) 72, 409, 532, 936
BspCNI CTCAG 2 cut(s) 132, 572
BspLI GGNNCC 1 cut(s) 883
BspMAI CTGCAG 1 cut(s) 107
BspPI GGATC 2 cut(s) 320, 651
BsrDI GCAATG 1 cut(s) 118
BsrI ACTGG 1 cut(s) 346
BssECI CCNNGG 3 cut(s) 73, 462, 942
BssMI GATC 5 cut(s) 39, 312, 643, 753, 987
BssSI CACGAG 1 cut(s) 612
BssT1I CCWWGG 1 cut(s) 73
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 4 cut(s) 264, 411, 464, 943
Bst4CI ACNGT 3 cut(s) 46, 353, 878
BstAPI GCANNNNNTGC 2 cut(s) 120, 554
BstDEI CTNAG 2 cut(s) 140, 559
BstF5I GGATG 1 cut(s) 962
BstHHI GCGC 2 cut(s) 519, 959
BstKTI GATC 5 cut(s) 42, 315, 646, 756, 990
BstMAI GTCTC 2 cut(s) 261, 440
BstMBI GATC 5 cut(s) 39, 312, 643, 753, 987
BstMWI GCNNNNNNNGC 4 cut(s) 111, 120, 554, 828
BstNI CCWGG 4 cut(s) 264, 411, 464, 943
BstNSI RCATGY 1 cut(s) 248
BstPAI GACNNNNGTC 1 cut(s) 14
BstSCI CCNGG 4 cut(s) 262, 409, 462, 941
BstSFI CTRYAG 3 cut(s) 103, 360, 874
BstV1I GCAGC 3 cut(s) 117, 541, 834
BsuRI GGCC 4 cut(s) 72, 409, 532, 936
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 1 cut(s) 962
BtsI GCAGTG 1 cut(s) 832
BtsIMutI CAGTG 2 cut(s) 832, 883
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 2 cut(s) 519, 959
Cfr13I GGNCC 2 cut(s) 407, 657
CviAII CATG 9 cut(s) 37, 206, 212, 245, 416, 528, 586, 635, 837
DdeI CTNAG 2 cut(s) 140, 559
DpnI GATC 5 cut(s) 41, 314, 645, 755, 989
DpnII GATC 5 cut(s) 39, 312, 643, 753, 987
DraIII CACNNNGTG 1 cut(s) 839
EaeI YGGCCR 2 cut(s) 70, 530
Eco130I CCWWGG 1 cut(s) 73
Eco24I GRGCYC 2 cut(s) 788, 949
Eco47I GGWCC 1 cut(s) 657
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 4 cut(s) 262, 409, 462, 941
EcoT14I CCWWGG 1 cut(s) 73
EcoT38I GRGCYC 2 cut(s) 788, 949
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 9 cut(s) 40, 209, 215, 248, 419, 531, 589, 638, 840
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 1 cut(s) 22
FatI CATG 9 cut(s) 36, 205, 211, 244, 415, 527, 585, 634, 836
FbaI TGATCA 1 cut(s) 39
Fnu4HI GCNGC 3 cut(s) 106, 555, 823
FokI GGATG 1 cut(s) 949
FriOI GRGCYC 2 cut(s) 788, 949
Fsp4HI GCNGC 3 cut(s) 106, 555, 823
FspAI RTGCGCAY 1 cut(s) 518
FspI TGCGCA 1 cut(s) 518
GlaI GCGC 2 cut(s) 518, 958
GluI GCNGC 3 cut(s) 106, 555, 823
GsuI CTGGAG 2 cut(s) 285, 363
HaeIII GGCC 4 cut(s) 72, 409, 532, 936
HhaI GCGC 2 cut(s) 519, 959
Hin1II CATG 9 cut(s) 40, 209, 215, 248, 419, 531, 589, 638, 840
Hin6I GCGC 2 cut(s) 517, 957
HinP1I GCGC 2 cut(s) 517, 957
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 5 cut(s) 59, 91, 288, 458, 471
HphI GGTGA 3 cut(s) 34, 825, 862
Hpy166II GTNNAC 3 cut(s) 34, 217, 250
Hpy188I TCNGA 4 cut(s) 141, 440, 562, 745
Hpy188III TCNNGA 3 cut(s) 173, 612, 772
Hpy8I GTNNAC 3 cut(s) 34, 217, 250
HpyCH4III ACNGT 3 cut(s) 46, 353, 878
HpyCH4IV ACGT 6 cut(s) 30, 177, 252, 396, 861, 915
HpyCH4V TGCA 5 cut(s) 105, 225, 794, 800, 822
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 120, 554, 828
HpyF3I CTNAG 2 cut(s) 140, 559
HpySE526I ACGT 6 cut(s) 30, 177, 252, 396, 861, 915
Hsp92II CATG 9 cut(s) 40, 209, 215, 248, 419, 531, 589, 638, 840
HspAI GCGC 2 cut(s) 517, 957
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 5 cut(s) 39, 312, 643, 753, 987
LmnI GCTCC 2 cut(s) 77, 881
Lsp1109I GCAGC 3 cut(s) 117, 541, 834
LweI GCATC 2 cut(s) 528, 963
MaeII ACGT 6 cut(s) 30, 177, 252, 396, 861, 915
MaeIII GTNAC 7 cut(s) 154, 178, 232, 253, 388, 397, 725
MalI GATC 5 cut(s) 41, 314, 645, 755, 989
MboI GATC 5 cut(s) 39, 312, 643, 753, 987
MboII GAAGA 2 cut(s) 453, 760
MhlI GDGCHC 2 cut(s) 788, 949
MlsI TGGCCA 1 cut(s) 72
MluCI AATT 6 cut(s) 160, 168, 535, 595, 730, 927
MluNI TGGCCA 1 cut(s) 72
MlyI GAGTC 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 8 cut(s) 135, 448, 568, 568, 747, 910, 976, 990
Mox20I TGGCCA 1 cut(s) 72
MscI TGGCCA 1 cut(s) 72
MseI TTAA 3 cut(s) 591, 750, 780
MslI CAYNNNNRTG 1 cut(s) 210
Msp20I TGGCCA 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 554
MspR9I CCNGG 4 cut(s) 264, 411, 464, 943
MvaI CCWGG 4 cut(s) 264, 411, 464, 943
MwoI GCNNNNNNNGC 4 cut(s) 111, 120, 554, 828
NdeII GATC 5 cut(s) 39, 312, 643, 753, 987
NlaIII CATG 9 cut(s) 40, 209, 215, 248, 419, 531, 589, 638, 840
NlaIV GGNNCC 1 cut(s) 883
NmuCI GTSAC 4 cut(s) 154, 253, 388, 725
NsbI TGCGCA 1 cut(s) 518
NspI RCATGY 1 cut(s) 248
PciI ACATGT 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PfeI GAWTC 4 cut(s) 59, 288, 458, 471
PflFI GACNNNGTC 1 cut(s) 152
PflMI CCANNNNNTGG 1 cut(s) 416
PkrI GCNGC 3 cut(s) 107, 556, 824
PleI GAGTC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 99
PscI ACATGT 1 cut(s) 244
PshAI GACNNNNGTC 1 cut(s) 14
Psp1406I AACGTT 1 cut(s) 396
Psp6I CCWGG 4 cut(s) 262, 409, 462, 941
PspGI CCWGG 4 cut(s) 262, 409, 462, 941
PspN4I GGNNCC 1 cut(s) 883
PspPI GGNCC 2 cut(s) 407, 657
PstI CTGCAG 1 cut(s) 107
PstNI CAGNNNCTG 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 152
RseI CAYNNNNRTG 1 cut(s) 210
SaqAI TTAA 3 cut(s) 591, 750, 780
SatI GCNGC 3 cut(s) 106, 555, 823
Sau3AI GATC 5 cut(s) 39, 312, 643, 753, 987
Sau96I GGNCC 2 cut(s) 407, 657
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 4 cut(s) 264, 411, 464, 943
SduI GDGCHC 2 cut(s) 788, 949
SfaNI GCATC 2 cut(s) 528, 963
SfcI CTRYAG 3 cut(s) 103, 360, 874
SinI GGWCC 1 cut(s) 657
SmiMI CAYNNNNRTG 1 cut(s) 210
Sse9I AATT 6 cut(s) 160, 168, 535, 595, 730, 927
SsiI CCGC 1 cut(s) 552
SspI AATATT 1 cut(s) 280
StyD4I CCNGG 4 cut(s) 262, 409, 462, 941
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 3 cut(s) 46, 353, 878
TaiI ACGT 6 cut(s) 33, 180, 255, 399, 864, 918
TaqI TCGA 1 cut(s) 147
TasI AATT 6 cut(s) 160, 168, 535, 595, 730, 927
TfiI GAWTC 4 cut(s) 59, 288, 458, 471
Tru1I TTAA 3 cut(s) 591, 750, 780
Tru9I TTAA 3 cut(s) 591, 750, 780
TscAI CASTG 2 cut(s) 832, 883
TseFI GTSAC 4 cut(s) 154, 253, 388, 725
TseI GCWGC 3 cut(s) 105, 554, 822
Tsp45I GTSAC 4 cut(s) 154, 253, 388, 725
TspGWI ACGGA 2 cut(s) 729, 958
TspRI CASTG 2 cut(s) 832, 883
Tth111I GACNNNGTC 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 416
VpaK11BI GGWCC 1 cut(s) 657
XapI RAATTY 2 cut(s) 160, 535
XceI RCATGY 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.