MD17G1110800.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000434
Physical Location & Seq
Reverse (-)
9526468 .. 9527576
1109 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1110800.v1.1.491

Sequence Viewer

Length: 828 bp
ATGCAAAAAGTCAGAAGTTTTTTTCTGAAATTTGCTTCCGAATGTTTTTCCCCGTCTAAAACGTTAATCCTTGTTCTAGTTATATATGATATGAAATTTATTGAATTGAAACTAATTGTTTTATTTTATTTTAATGCAGCTGGAAATTGGATATTGGCATTCTTATTTTTAGTATCCATTGCTAAAGCCGAGGTCTTTTATGTGACAAGTTTAAGATATCGAGGAAATTCTAAGGGTGACATTACGCAGGCTGTGGCTAACGCTTGGAGAGATGCATGTGCATCACCATGGCCAGCCAAAGTTATTATTCCACATTGGGAATATTATTTAAGAGGAGCTATTCTGAAAGGTCCTTGCAAAGCTCCGATTGAGATTCAAGTTCAAGGCTATTTACGTGCCCCTCAAGACCGCAGCCAACTCATTCAGCAAGGCACCTGGGTTCGATTTCAGCACGTTAATAGGCTCATGTTGTTTGGTGGTGGAACTTTTGATGGCCAAAATAATTGCTACCAAAATCTGATGTTTGATTTTGTCACCAATTCCATAATCAAGGGTATAACTTCACTTGATAGAAAATATTTCTACATCAATGCTCATGAATGTCGCAATGTCACACTACAATACTTGACCATCACAGCGCCGGAGGACAGCCACAACACGGATGGAATTCACATCGGGAGTTCATTCGGGATAACCATCAATCACACAACAATTATTGGAACCGGAGACGATTGTGTTTCTGTTATTGGTGGGAGCAAAAACATCATCGCGACCAACTTTATTTGTGGACCAGGGCACGGCATTGATAGACATATATTGTGTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

276

Amino Acids

30.86

Weight (kDa)

8.86

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 93 - 268 2.4e-37 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000434)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07820 AT3G07830 AT3G07840 AT5G48140
fragaria_vesca FvH4_1g26811 FvH4_2g02350 FvH4_6g41380 FvH4_6g41380 FvH4_6g41380 FvH4_6g41430 FvH4_6g41740 FvH4_6g43060 FvH4_7g02910
malus_domestica MD00G1117700.v1.1 MD00G1117800.v1.1 MD00G1117900.v1.1 MD09G1120000.v1.1 MD09G1120100.v1.1 MD09G1120200.v1.1 MD09G1120300.v1.1 MD12G1142000.v1.1 MD13G1218300.v1.1 MD13G1220100.v1.1 MD13G1220200.v1.1 MD16G1223300.v1.1 MD16G1223500.v1.1 MD16G1223600.v1.1 MD17G1110800.v1.1
prunus_persica Prupe.1G044900_v2.0.a1 Prupe.1G045000_v2.0.a1 Prupe.1G167700_v2.0.a1 Prupe.2G005600_v2.0.a1 Prupe.2G005700_v2.0.a1 Prupe.2G008600_v2.0.a1 Prupe.2G009200_v2.0.a1 Prupe.2G009300_v2.0.a1 Prupe.2G009400_v2.0.a1 Prupe.3G109100_v2.0.a1 Prupe.3G109200_v2.0.a1 Prupe.3G109400_v2.0.a1 Prupe.3G109500_v2.0.a1 Prupe.3G109700_v2.0.a1 Prupe.3G203300_v2.0.a1 Prupe.3G203600_v2.0.a1 Prupe.3G203900_v2.0.a1 Prupe.3G204000_v2.0.a1 Prupe.3G204100_v2.0.a1 Prupe.3G204200_v2.0.a1
pyrus_communis pycom09g04480 pycom09g04490 pycom09g04500 pycom12g13680
rosa_chinensis RchiOBHm_Chr1g0323871 RchiOBHm_Chr2g0156921 RchiOBHm_Chr2g0159061 RchiOBHm_Chr6g0246271 RchiOBHm_Chr6g0246281
rosa_laevigata RLG00000015266 RLG00000020954 RLG00000021134 RLG00000030246 RLG00000030250 RLG00000030295
rosa_multiflora Rmu_co8098394.1_g000001 Rmu_co8404671.1_g000001 Rmu_sc0000507.1_g000021 Rmu_sc0001066.1_g000011 Rmu_sc0001066.1_g000015 Rmu_sc0009451.1_g000001 Rmu_ssc0000408.1_g000005
rosa_roxburghii Rroxscaffold_2G00090810 Rroxscaffold_2G00092580 Rroxscaffold_4G00326280 Rroxscaffold_7G00214720
rosa_rugosa Rorug01G0040400 Rorug02G0457200 Rorug05G0520000
rosa_samantha Rh1AG057700 Rh1BG048800 Rh1CG059100 Rh1DG063000 Rh2AG522900 Rh2BG536200 Rh2BG553200 Rh2CG507800 Rh2DG545200 Rh6AG034100 Rh6BG029300 Rh6DG028700
rosa_wichuraiana Rw0G023110 Rw1G004930 Rw2G043170 Rw2G044730 Rw6G002760 Rw6G002920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 431
AccII CGCG 1 cut(s) 770
AciI CCGC 1 cut(s) 409
AclI AACGTT 1 cut(s) 62
AcoI YGGCCR 2 cut(s) 290, 493
AcsI RAATTY 4 cut(s) 29, 95, 226, 666
AfiI CCNNNNNNNGG 2 cut(s) 658, 797
AgsI TTSAA 4 cut(s) 104, 109, 377, 383
AjnI CCWGG 2 cut(s) 434, 790
AluBI AGCT 3 cut(s) 140, 338, 362
AluI AGCT 3 cut(s) 140, 338, 362
Alw26I GTCTC 1 cut(s) 720
AoxI GGCC 2 cut(s) 290, 493
ApeKI GCWGC 2 cut(s) 137, 411
ApoI RAATTY 4 cut(s) 29, 95, 226, 666
AspLEI GCGC 1 cut(s) 640
AspS9I GGNCC 2 cut(s) 350, 788
AsuHPI GGTGA 3 cut(s) 248, 276, 526
AvaII GGWCC 2 cut(s) 350, 788
BaeGI GKGCMC 2 cut(s) 400, 798
BalI TGGCCA 2 cut(s) 292, 495
BanI GGYRCC 1 cut(s) 431
BbvI GCAGC 2 cut(s) 149, 423
BccI CCATC 4 cut(s) 485, 638, 656, 704
BceAI ACGGC 1 cut(s) 814
BciT130I CCWGG 2 cut(s) 436, 792
BciVI GTATCC 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 720
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 1 cut(s) 641
BfuI GTATCC 1 cut(s) 184
BisI GCNGC 2 cut(s) 138, 412
BlsI GCNGC 2 cut(s) 139, 413
Bme1390I CCNGG 2 cut(s) 436, 792
Bme18I GGWCC 2 cut(s) 350, 788
BmgT120I GGNCC 2 cut(s) 350, 788
BmiI GGNNCC 2 cut(s) 433, 721
BmrFI CCNGG 2 cut(s) 436, 792
BmsI GCATC 2 cut(s) 262, 290
BpuEI CTTGAG 1 cut(s) 387
BsaAI YACGTR 1 cut(s) 395
BsaBI GATNNNNATC 1 cut(s) 695
BsaJI CCNNGG 4 cut(s) 189, 287, 435, 791
BsaWI WCCGGW 1 cut(s) 722
Bsc4I CCNNNNNNNGG 2 cut(s) 658, 797
Bse3DI GCAATG 2 cut(s) 177, 613
Bse8I GATNNNNATC 1 cut(s) 695
BseBI CCWGG 2 cut(s) 436, 792
BseDI CCNNGG 4 cut(s) 189, 287, 435, 791
BseGI GGATG 1 cut(s) 667
BseJI GATNNNNATC 1 cut(s) 695
BseLI CCNNNNNNNGG 2 cut(s) 658, 797
BseMI GCAATG 2 cut(s) 177, 613
BseRI GAGGAG 1 cut(s) 348
BseSI GKGCMC 2 cut(s) 400, 798
BseXI GCAGC 2 cut(s) 149, 423
Bsh1236I CGCG 1 cut(s) 770
BshFI GGCC 2 cut(s) 292, 495
BshNI GGYRCC 1 cut(s) 431
BsiSI CCGG 2 cut(s) 641, 723
BslI CCNNNNNNNGG 2 cut(s) 658, 797
BsmAI GTCTC 1 cut(s) 720
BsmBI CGTCTC 1 cut(s) 720
BsmI GAATGC 1 cut(s) 158
BsnI GGCC 2 cut(s) 292, 495
Bsp1286I GDGCHC 2 cut(s) 400, 798
Bsp19I CCATGG 1 cut(s) 287
Bsp68I TCGCGA 1 cut(s) 770
BspACI CCGC 1 cut(s) 409
BspANI GGCC 2 cut(s) 292, 495
BspFNI CGCG 1 cut(s) 770
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 433, 721
BspT107I GGYRCC 1 cut(s) 431
BsrDI GCAATG 2 cut(s) 177, 613
BssECI CCNNGG 4 cut(s) 189, 287, 435, 791
BssT1I CCWWGG 1 cut(s) 287
Bst2UI CCWGG 2 cut(s) 436, 792
BstBAI YACGTR 1 cut(s) 395
BstC8I GCNNGC 2 cut(s) 249, 294
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 667
BstFNI CGCG 1 cut(s) 770
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 1 cut(s) 640
BstMAI GTCTC 1 cut(s) 720
BstNI CCWGG 2 cut(s) 436, 792
BstNSI RCATGY 1 cut(s) 279
BstSCI CCNGG 2 cut(s) 434, 790
BstSLI GKGCMC 2 cut(s) 400, 798
BstUI CGCG 1 cut(s) 770
BstV1I GCAGC 2 cut(s) 149, 423
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 2 cut(s) 292, 495
BtgI CCRYGG 1 cut(s) 287
BtgZI GCGATG 1 cut(s) 751
BtsCI GGATG 1 cut(s) 667
BtuMI TCGCGA 1 cut(s) 770
Cac8I GCNNGC 2 cut(s) 249, 294
CciI TCATGA 1 cut(s) 595
CfoI GCGC 1 cut(s) 640
Cfr13I GGNCC 2 cut(s) 350, 788
CviAII CATG 4 cut(s) 276, 288, 466, 596
DdeI CTNAG 1 cut(s) 231
EaeI YGGCCR 2 cut(s) 290, 493
Eco130I CCWWGG 1 cut(s) 287
Eco32I GATATC 1 cut(s) 218
Eco47I GGWCC 2 cut(s) 350, 788
EcoO109I RGGNCCY 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 666
EcoRII CCWGG 2 cut(s) 434, 790
EcoRV GATATC 1 cut(s) 218
EcoT14I CCWWGG 1 cut(s) 287
EcoT22I ATGCAT 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 287
Esp3I CGTCTC 1 cut(s) 720
FaeI CATG 4 cut(s) 279, 291, 469, 599
FatI CATG 4 cut(s) 275, 287, 465, 595
Fnu4HI GCNGC 2 cut(s) 138, 412
FokI GGATG 1 cut(s) 674
Fsp4HI GCNGC 2 cut(s) 138, 412
FspBI CTAG 1 cut(s) 77
GlaI GCGC 1 cut(s) 639
GluI GCNGC 2 cut(s) 138, 412
HaeII RGCGCY 1 cut(s) 641
HaeIII GGCC 2 cut(s) 292, 495
HapII CCGG 2 cut(s) 641, 723
HhaI GCGC 1 cut(s) 640
Hin1II CATG 4 cut(s) 279, 291, 469, 599
Hin6I GCGC 1 cut(s) 638
HinP1I GCGC 1 cut(s) 638
HinfI GANTC 1 cut(s) 373
HpaII CCGG 2 cut(s) 641, 723
HphI GGTGA 3 cut(s) 248, 276, 526
Hpy166II GTNNAC 1 cut(s) 788
Hpy188I TCNGA 6 cut(s) 14, 27, 40, 345, 366, 519
Hpy188III TCNNGA 5 cut(s) 404, 596, 676, 688, 769
Hpy8I GTNNAC 1 cut(s) 788
HpyCH4IV ACGT 3 cut(s) 62, 394, 453
HpyCH4V TGCA 5 cut(s) 4, 137, 275, 281, 357
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 3 cut(s) 62, 394, 453
Hsp92II CATG 4 cut(s) 279, 291, 469, 599
HspAI GCGC 1 cut(s) 638
LmnI GCTCC 3 cut(s) 335, 367, 753
LpnPI CCDG 9 cut(s) 126, 233, 306, 421, 448, 654, 736, 777, 804
Lsp1109I GCAGC 2 cut(s) 149, 423
LweI GCATC 2 cut(s) 262, 290
MaeI CTAG 1 cut(s) 77
MaeII ACGT 3 cut(s) 62, 394, 453
MaeIII GTNAC 4 cut(s) 202, 236, 532, 610
MhlI GDGCHC 2 cut(s) 400, 798
MlsI TGGCCA 2 cut(s) 292, 495
MluNI TGGCCA 2 cut(s) 292, 495
MnlI CCTC 5 cut(s) 184, 215, 326, 411, 637
Mox20I TGGCCA 2 cut(s) 292, 495
Mph1103I ATGCAT 1 cut(s) 277
MscI TGGCCA 2 cut(s) 292, 495
MseI TTAA 5 cut(s) 65, 132, 212, 329, 456
MslI CAYNNNNRTG 1 cut(s) 286
Msp20I TGGCCA 2 cut(s) 292, 495
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 2 cut(s) 641, 723
MspR9I CCNGG 2 cut(s) 436, 792
Mva1269I GAATGC 1 cut(s) 158
MvaI CCWGG 2 cut(s) 436, 792
MvnI CGCG 1 cut(s) 770
NcoI CCATGG 1 cut(s) 287
NlaIII CATG 4 cut(s) 279, 291, 469, 599
NlaIV GGNNCC 2 cut(s) 433, 721
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 4 cut(s) 202, 236, 532, 610
NruI TCGCGA 1 cut(s) 770
NsiI ATGCAT 1 cut(s) 277
NspI RCATGY 1 cut(s) 279
PagI TCATGA 1 cut(s) 595
PctI GAATGC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 373
PkrI GCNGC 2 cut(s) 139, 413
Ppu21I YACGTR 1 cut(s) 395
PpuMI RGGWCCY 1 cut(s) 350
Psp1406I AACGTT 1 cut(s) 62
Psp5II RGGWCCY 1 cut(s) 350
Psp6I CCWGG 2 cut(s) 434, 790
PspGI CCWGG 2 cut(s) 434, 790
PspN4I GGNNCC 2 cut(s) 433, 721
PspPI GGNCC 2 cut(s) 350, 788
PspPPI RGGWCCY 1 cut(s) 350
PvuII CAGCTG 1 cut(s) 140
RruI TCGCGA 1 cut(s) 770
RseI CAYNNNNRTG 1 cut(s) 286
SaqAI TTAA 5 cut(s) 65, 132, 212, 329, 456
SatI GCNGC 2 cut(s) 138, 412
Sau96I GGNCC 2 cut(s) 350, 788
ScrFI CCNGG 2 cut(s) 436, 792
SduI GDGCHC 2 cut(s) 400, 798
SetI ASST 9 cut(s) 65, 142, 195, 340, 352, 364, 397, 437, 456
SfaNI GCATC 2 cut(s) 262, 290
SinI GGWCC 2 cut(s) 350, 788
SmiMI CAYNNNNRTG 1 cut(s) 286
SmlI CTYRAG 1 cut(s) 402
SmoI CTYRAG 1 cut(s) 402
SsiI CCGC 1 cut(s) 409
SspI AATATT 2 cut(s) 323, 578
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 2 cut(s) 434, 790
StyI CCWWGG 1 cut(s) 287
TaiI ACGT 3 cut(s) 65, 397, 456
TaqI TCGA 2 cut(s) 220, 442
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 5 cut(s) 65, 132, 212, 329, 456
Tru9I TTAA 5 cut(s) 65, 132, 212, 329, 456
TseFI GTSAC 4 cut(s) 202, 236, 532, 610
TseI GCWGC 2 cut(s) 137, 411
Tsp45I GTSAC 4 cut(s) 202, 236, 532, 610
TspDTI ATGAA 3 cut(s) 107, 612, 672
TspGWI ACGGA 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 350, 788
XapI RAATTY 4 cut(s) 29, 95, 226, 666
XceI RCATGY 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 659
XspI CTAG 1 cut(s) 77
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.