Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000355

Physical Location & Seq

Genomic Coordinates Forward (+)

6175213 .. 6185872

Gene Structure 10660 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1052400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3861 bp

ATGGGGATCGTAGAGACACTGCAATATTTGGAATCATCTAGAAGAATTGCTTCAGTTAATGATAGTCATTCTGAGGGAAGATTAGTACGTATGCCCATGGCTAATGGGGAGTGCTCTATCCGTACGCAGTCACCTAAGTTAAAGAGGCGCAAAATCTCCGCTGTTCGTGATTTTCCCCCGGGGTGTGGACGATTTGGTCAGTTAAGCAATTTGGGACCTGATAAGGAGGCCGCTTCTGTGGGTACTTTATTGACTGCTCTGACAGAAAGTTTGGTTAGTGGGGGTAAATATGGTGACGGACGTGGTGCTGAAAATCTGATGCCCTCAACTGGTCAGGTAGATGATACTGTTTTGATTAGTGGTTACGATGTTAGTACTGGTGAGACAGTTGAATCTTTAACAGCCTTAGAGCATGAAATATATGATTCGTTGAAGATCCAACATCAGCTTGGTGCTGCTGCGCCAGAGGAGGAGATAGTTGCAGTTCTGCCTGATAGAAATATTTGTTCACCACCTGACAGATCCATCTCTATTTCTACATGCAATCTTCTGGAGAAGACTGCAGTTAAAAAATATCCTCCTCGAAGAAAAATATCAGCCGTTCGTGACTTACCTCCTCTGTGTGGAAGAAATGCTTCCCTGGAAGTGAGGAAATTTGGTCAAGAAAAATCGCTTATCGATGGTGAACCATCATCATTGAACACAGTGAAGACCGATGTGAAGCAAACATGTGAGGATGTCTATGATGAAGAGTTTCACAAGAGTGAATATGTAGAGAATGTATTAGAAATAAGTGGATCCGAGGTTCAACTAAATTGCAATGGGCATGTTGTACAAAAAATGGAGAGAGAAGATGAATATAAAGTAAATTCGAAGATAAACGTGGTTTTGGAAGGTACAAGAAAAACATGCATTGAACCTTCTCAAAAAAGTAACGGATGTCTACGCACAAAGGATGTTGAGCATTCAGAGGAGAAAGTGGAGGACAAGATGGAGGTATACCAAGCAGAGAAAAGTACGAGTGAAAAATGTCTGGAAGTATCTTATTATAATAACCAATTGCACGAAGAAGATTTTGTAAGTTTGGAACCTACATCGAATAGTGAGATTGTGCTGGGTTTGATGGCTGCGTCAAATTGTCCATGGAGGAAGGGAAAAGGGGTTTTCAAGCGTAAATCAGAAGGCGGTGTGAGTGTAAGAAAACCCTCTCAACAAAGCAATGGATGCCAAGGCATGGGGGATATTGGACTTTCAGAGGAGATAGTGGGGACGGAGATGGTGGTATACCAAGCGAAGAAAAGTCCGGGTAAAAAATGTATAGATGTATCTTATTATCATAACCAATTGCACAAAGAAGTTCTTGGAATTTCAGAACGTACTATGGATAGGGTGGTTGTGCTGGGTTTGATGGCTGCGTCAAACTGTCCATGGAGGAAGGAAAAAGAGGTCTCCATGCGTAAACCAGAAGATGGTGCAAGTGAAAGTAAACCTTCTCAAGAAAGCAATGGATGCCAAGGCATAGGGGGTGTTGGACATTCAGAGGAGATAGTGGGGAAGGAGATGGTGGTATACCAAGCGAAGAGAAGTCCAGGTGAAAAATGTATAGATGTATCTTATTATCATAACCAATTGCACAAAGAAGTTCTTGGAATTTCAGAACCTACTATGGATAGGGTGGTTGTGCTGGGTTTGATGGCTGCGTCAAACTGCCCATGGAGGAAGGGAAAAGAGGTCTCCATGCGTAAACCAGAAGATGGTGCAAGTGAAAGTAAACCTTCTCAAGAAAGCAATGGATGCCAAGGCATGGGGGGTGTTGGACATTCAGAGGAGAAAGTTGGGAAGGAGATGGTGGTATACCAAGCGAAGGAGACTCTGGGTGAAACATGTTTGGAAGTATCTTATTATAATAACCAATTGCACAAAGAAGATTTTGAAAGTTCGGAACTTACTTCAGATACAGTGGTTGTGCTGGGTTTGATGGCTGCATCTAATTGTCCGTGGAGGAAGGGAAAAAGGGTCTCCAAGCACAAAGCAAAAGGTGGTACAAGTGAAAGCGAACAAAACAAACTTGATTTGAAATGCCAACTGGAAGGATCTAGTACTGTGTCAACAAAAGTTGACTCAGACATTGGAAGAAAGTCTGTAAAGAAGATTTTTCGTAAAGCACGAAAGAGTGCTTATCAAGGTACAAGTCTGTTCTGGGATGAGGAGTATTCTCTCGAGCATGACCCAGAGGATCTTCATGTGACTCCAAGATCATGTTATTCAGATGTATCCCCTCATCTGTTTAGTCCTAGTAGTGCAACTAGTAAAAATAATGAGAACAACGCAATTGTCAACCGACACACGGTGAAGGAGACATTGCATCTGTTCCAGGCTCTTTGTAGGAAGCTCTTGCAGGAGGAAGAGGGTAAGTCAAAGGAAGGAGGAATTTCTCGCCAAAAAATTGATTATTCAGCTTTAAAGATCCTCAGGGATAAGGGAAAATATGTAAATATGGGCAAACACATTGGAGCTGTCCCAGGAGTTGAAGTTGGTGATGAGTTTCATAACCGAGTGGAACTTACTATTGTTGGCCTTCATCGGCTGACTCAGGGTGGTATAGATTATGTGAATCATTGTGGCAAGATCCTTGCAACTAGTATCATTGCATCTGGTGGCTATGCTGATGATTTGAATGATTCAAATTCCTTGATTTATACAGGCCAAGGAGGAAATGTGATGAAGACTAATAAGGAACCTGAAGATCAGAAGCTTGAACGGGGAAACCTTGCTTTGGATAATAGTCTGCATGAAAGGAACCCTGTTAGAGTGATTCGTGGCTCTGAATCCTCAGATGGAAAGAGTAAGACGTATGTATATGATGGACTATATTTGGTAAAGAAACGTTGGCAGGAATTGGGGTCTCATGGTAAGCTTGTTTTCAAGTTTCAACTGGACAAAATTCGAGACCAACACCTTGCTCGGAAAGAAGTGAAGAAGTCCAAAATAATTCAAGTTCAGGAAGGTCTCTGCATTGATGATATTTCACTAGGGAAAGAGTTAATTCCTGTTTCTGCTGTGAATACCATAGATGATGAGAAACCTCCAACATTTGTATATGTAACTAGTATGCTATATCCTGATTGGTGCCGTCCAATTCCTCTCAAGGGTTGCAGCTGTATTGTTGAATGCTCAGATTCTGAGAAATGTTCTTGTGCAGTTCAGAACGGAGGTGAGATCCCGTATAACTTCGACGGTGCTATTGTTGAAGTAAAGCCCCTTGTGTATGAGTGTGGTCCTTCTTGCAAGTGCCCTCCGTCTTGTTACAATAGAGTTGGTCAACATGGTATCAAATTTCAGCTTCAAATTTTTAAAACAGAATCAAGGGGTTGGGGATTGAGATCACTAAATTACATTCCTTCAGGAAGTTTTATATGTGAGTATATGGGAGAGCTCCTTGAAGAGGACAAAGCTGAAGAAAGAATTGGTTATGATGAGTATCTGTTTGATATTGGGAATAACTACAATGACAATAATCTTTGGGATGAACTTTCAACCCTTGTGCCCGATGCGCTGTCAAGTTCTTTTGAAGTTGTGGACGATGGTAAATTCACCATTGATGCAGCAAACTATGGAAATGTGGGAAGATTTATTAACCATAGTTGTTCCCCTAATCTTTATGCCCAAGATGTTCTCTACGATCACAATGACAATAGAATTCCTCACATTATGTTCTTTGCCGCTGAAGACATTCCCCCGATGCAAGAGTTGACTTACCATTACAATTATACGATAGATTCGGTTTATGATTCCAATGGCAATATAAAGAAGAAGAATTGCTATTGTGGTTCGCTGGACTGTACTGGCAGGCTGTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0005488 GO:0006325 GO:0006342 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008276 GO:0008327 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0031047 GO:0031048 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0036094 GO:0036211 GO:0040029 GO:0042054 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0043414 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045814 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko00310 ko04211 map00310 map04211

Pfam Domains

Protein Families

Pre-SET SAD_SRA SET

Protein Analysis

1287

Amino Acids

142.82

Weight (kDa)

5.78

Isoelectric Point (pI)

40.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SAD_SRA	PF02182	836 - 985	3.1e-51	SAD/SRA domain
Pre-SET	PF05033	1012 - 1105	7.8e-22	Pre-SET motif
SET	PF00856	1124 - 1256	5.1e-23	SET domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000355)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22740 AT2G22740 AT2G35160 AT2G35160 AT2G35160 AT2G35160 AT2G35160
fragaria_vesca	FvH4_5g20100 FvH4_5g20130 FvH4_5g20130 FvH4_5g20130 FvH4_5g20130 FvH4_5g20130 FvH4_5g20150 FvH4_5g25380 FvH4_5g30731 FvH4_5g30740
malus_domestica	MD03G1258900.v1.1 MD04G1052400.v1.1 MD11G1279700.v1.1
prunus_persica	Prupe.2G064400_v2.0.a1 Prupe.5G057000_v2.0.a1 Prupe.5G057400_v2.0.a1
pyrus_communis	pycom03g20690 pycom04g04620 pycom11g24730
rosa_chinensis	RchiOBHm_Chr7g0204991 RchiOBHm_Chr7g0205081 RchiOBHm_Chr7g0205121 RchiOBHm_Chr7g0205131 RchiOBHm_Chr7g0215601 RchiOBHm_Chr7g0225631
rosa_laevigata	RLG00000001799 RLG00000002623 RLG00000002644 RLG00000003398 RLG00000003403 RLG00000003417 RLG00000034641
rosa_multiflora	Rmu_co8235369.1_g000001 Rmu_co8353775.1_g000001 Rmu_co8458607.1_g000001 Rmu_sc0000898.1_g000043 Rmu_sc0000898.1_g000048 Rmu_sc0001072.1_g000001 Rmu_sc0001072.1_g000002 Rmu_sc0003876.1_g000010 Rmu_sc0005112.1_g000021 Rmu_sc0007602.1_g000010 Rmu_sc0009349.1_g000014 Rmu_sc0020855.1_g000001 Rmu_sc0023402.1_g000001 Rmu_sc0024226.1_g000004
rosa_roxburghii	Rroxscaffold_3G00233620 Rroxscaffold_3G00244060 Rroxscaffold_3G00252560 Rroxscaffold_3G00252590 Rroxscaffold_3G00252620
rosa_rugosa	Rorug07G0086900 Rorug07G0087000 Rorug07G0087000 Rorug07G0087400 Rorug07G0087500 Rorug07G0087600 Rorug07G0087700 Rorug07G0087800 Rorug07G0088100 Rorug07G0095600.1 Rorug07G0095700.1 Rorug07G0095800.1 Rorug07G0095900 Rorug07G0096000 Rorug07G0096100 Rorug07G0155000 Rorug07G0232100
rosa_samantha	Rh7AG217900 Rh7AG218400 Rh7AG219000 Rh7AG291200 Rh7AG371700 Rh7BG214800 Rh7BG215000 Rh7BG215500 Rh7BG223800 Rh7BG224400 Rh7BG283300 Rh7BG283800 Rh7BG366000 Rh7CG231100 Rh7CG231600 Rh7CG232000 Rh7CG241100 Rh7CG311200 Rh7CG390400 Rh7DG225200 Rh7DG225900 Rh7DG226400 Rh7DG295000 Rh7DG376800
rosa_wichuraiana	Rw0G004730 Rw7G018880 Rw7G018900 Rw7G018920 Rw7G018940 Rw7G018960 Rw7G024860 Rw7G031790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1050, 1905
AasI	GACNNNNNNGTC	1 cut(s)	195
AccB1I	GGYRCC	1 cut(s)	3129
AccB7I	CCANNNNNTGG	4 cut(s)	1234, 1469, 1754, 1804
AccI	GTMKAC	5 cut(s)	943, 999, 1284, 1569, 1854
AciI	CCGC	4 cut(s)	159, 231, 1185, 3723
AclI	AACGTT	1 cut(s)	2887
AcuI	CTGAAG	6 cut(s)	36, 1935, 2763, 3387, 3477, 3747
AdeI	CACNNNGTG	1 cut(s)	2350
AflIII	ACRYGT	2 cut(s)	728, 1883
AhlI	ACTAGT	3 cut(s)	2306, 2639, 3107
AjiI	CACGTC	1 cut(s)	302
AjnI	CCWGG	4 cut(s)	639, 1588, 2373, 2521
AjuI	GAANNNNNNNTTGG	2 cut(s)	3450, 3482
AleI	CACNNNNGTG	1 cut(s)	762
AloI	GAACNNNNNNTCC	2 cut(s)	789, 821
Alw21I	GWGCWC	2 cut(s)	116, 3438
AlwNI	CAGNNNCTG	1 cut(s)	3182
Ama87I	CYCGRG	2 cut(s)	178, 2219
AoxI	GGCC	3 cut(s)	228, 2575, 2704
ApeKI	GCWGC	8 cut(s)	455, 458, 1127, 1412, 1697, 1982, 3156, 3605
ArsI	GACNNNNNNTTYG	2 cut(s)	253, 285
Asp700I	GAANNNNTTC	4 cut(s)	49, 634, 753, 3732
AspLEI	GCGC	3 cut(s)	150, 463, 3556
AspS9I	GGNCC	2 cut(s)	215, 3278
AsuC2I	CCSGG	3 cut(s)	179, 180, 1305
AsuII	TTCGAA	1 cut(s)	872
AvaI	CYCGRG	2 cut(s)	178, 2219
AvaII	GGWCC	2 cut(s)	215, 3278
AxyI	CCTNAGG	1 cut(s)	2471
BaeGI	GKGCMC	2 cut(s)	3296, 3549
BaeI	ACNNNNGTAYC	2 cut(s)	1947, 1980
BamHI	GGATCC	1 cut(s)	797
BanI	GGYRCC	1 cut(s)	3129
BanII	GRGCYC	1 cut(s)	3438
BbsI	GAAGAC	4 cut(s)	563, 716, 2732, 3735
Bbv12I	GWGCWC	2 cut(s)	116, 3438
BbvI	GCAGC	8 cut(s)	442, 445, 1114, 1399, 1684, 1969, 3168, 3617
BceAI	ACGGC	2 cut(s)	584, 3117
BciT130I	CCWGG	4 cut(s)	641, 1590, 2375, 2523
BciVI	GTATCC	1 cut(s)	2284
BcnI	CCSGG	3 cut(s)	179, 180, 1305
BcuI	ACTAGT	3 cut(s)	2306, 2639, 3107
BfaI	CTAG	7 cut(s)	39, 2097, 2295, 2307, 2640, 3032, 3108
BfmI	CTRYAG	1 cut(s)	561
BfuI	GTATCC	1 cut(s)	2284
BmcAI	AGTACT	2 cut(s)	376, 2101
Bme1390I	CCNGG	7 cut(s)	179, 180, 641, 1305, 1590, 2375, 2523
Bme18I	GGWCC	2 cut(s)	215, 3278
BmeT110I	CYCGRG	2 cut(s)	178, 2219
BmgBI	CACGTC	1 cut(s)	302
BmgT120I	GGNCC	2 cut(s)	215, 3278
BmiI	GGNNCC	6 cut(s)	216, 799, 1089, 2739, 2801, 3131
BmrFI	CCNGG	7 cut(s)	179, 180, 641, 1305, 1590, 2375, 2523
BpiI	GAAGAC	4 cut(s)	563, 716, 2732, 3735
BpmI	CTGGAG	1 cut(s)	572
Bpu14I	TTCGAA	1 cut(s)	872
BpuEI	CTTGAG	3 cut(s)	1479, 1764, 3131
BpuMI	CCSGG	3 cut(s)	179, 180, 1305
Bsa29I	ATCGAT	1 cut(s)	678
BsaAI	YACGTR	1 cut(s)	89
BsaBI	GATNNNNATC	2 cut(s)	3382, 3480
BsaI	GGTCTC	6 cut(s)	1453, 1738, 2023, 2910, 2943, 3014
BsaXI	ACNNNNNCTCC	4 cut(s)	2517, 2547, 2703, 2733
Bse1I	ACTGG	5 cut(s)	334, 382, 2091, 2940, 3850
Bse21I	CCTNAGG	1 cut(s)	2471
Bse3DI	GCAATG	6 cut(s)	826, 1225, 1510, 1795, 2360, 2646
Bse8I	GATNNNNATC	2 cut(s)	3382, 3480
BseBI	CCWGG	4 cut(s)	641, 1590, 2375, 2523
BseCI	ATCGAT	1 cut(s)	678
BseGI	GGATG	8 cut(s)	742, 944, 961, 1229, 1514, 1799, 2209, 3532
BseJI	GATNNNNATC	2 cut(s)	3382, 3480
BseMI	GCAATG	6 cut(s)	826, 1225, 1510, 1795, 2360, 2646
BseMII	CTCAG	7 cut(s)	63, 2136, 2485, 2606, 2847, 3174, 3189
BseNI	ACTGG	5 cut(s)	334, 382, 2091, 2940, 3850
BseRI	GAGGAG	9 cut(s)	482, 485, 570, 606, 986, 1271, 1556, 1841, 2222
BseSI	GKGCMC	2 cut(s)	3296, 3549
BseXI	GCAGC	8 cut(s)	442, 445, 1114, 1399, 1684, 1969, 3168, 3617
BseYI	CCCAGC	4 cut(s)	1114, 1399, 1684, 1969
BsgI	GTGCAG	1 cut(s)	3219
BshFI	GGCC	3 cut(s)	230, 2577, 2706
BshNI	GGYRCC	1 cut(s)	3129
BshVI	ATCGAT	1 cut(s)	678
BsiHKAI	GWGCWC	2 cut(s)	116, 3438
BsiHKCI	CYCGRG	2 cut(s)	178, 2219
BsiSI	CCGG	2 cut(s)	179, 1304
BsiWI	CGTACG	1 cut(s)	122
BslFI	GGGAC	3 cut(s)	228, 1282, 2504
BsmFI	GGGAC	3 cut(s)	228, 1282, 2504
BsmI	GAATGC	2 cut(s)	964, 3176
BsnI	GGCC	3 cut(s)	230, 2577, 2706
Bso31I	GGTCTC	6 cut(s)	1453, 1738, 2023, 2910, 2943, 3014
BsoBI	CYCGRG	2 cut(s)	178, 2219
Bsp119I	TTCGAA	1 cut(s)	872
Bsp1286I	GDGCHC	4 cut(s)	116, 3296, 3438, 3549
Bsp1407I	TGTACA	1 cut(s)	832
Bsp19I	CCATGG	4 cut(s)	96, 1142, 1427, 1712
BspACI	CCGC	4 cut(s)	159, 231, 1185, 3723
BspANI	GGCC	3 cut(s)	230, 2577, 2706
BspCNI	CTCAG	7 cut(s)	64, 2135, 2484, 2605, 2846, 3175, 3188
BspDI	ATCGAT	1 cut(s)	678
BspLI	GGNNCC	6 cut(s)	216, 799, 1089, 2739, 2801, 3131
BspMAI	CTGCAG	1 cut(s)	565
BspT104I	TTCGAA	1 cut(s)	872
BspT107I	GGYRCC	1 cut(s)	3129
BspTNI	GGTCTC	6 cut(s)	1453, 1738, 2023, 2910, 2943, 3014
BsrDI	GCAATG	6 cut(s)	826, 1225, 1510, 1795, 2360, 2646
BsrGI	TGTACA	1 cut(s)	832
BsrI	ACTGG	5 cut(s)	334, 382, 2091, 2940, 3850
BssNAI	GTATAC	4 cut(s)	1000, 1285, 1570, 1855
BssT1I	CCWWGG	8 cut(s)	96, 1142, 1228, 1427, 1513, 1712, 1798, 2707
Bst1107I	GTATAC	4 cut(s)	1000, 1285, 1570, 1855
Bst2UI	CCWGG	4 cut(s)	641, 1590, 2375, 2523
Bst4CI	ACNGT	9 cut(s)	349, 388, 706, 1424, 1960, 2104, 2350, 3239, 3842
Bst6I	CTCTTC	4 cut(s)	744, 1574, 2400, 3438
BstAPI	GCANNNNNTGC	3 cut(s)	1224, 1509, 1794
BstAUI	TGTACA	1 cut(s)	832
BstBAI	YACGTR	1 cut(s)	89
BstBI	TTCGAA	1 cut(s)	872
BstC8I	GCNNGC	1 cut(s)	3851
BstDEI	CTNAG	9 cut(s)	72, 135, 406, 2122, 2471, 2592, 2833, 3175, 3183
BstDSI	CCRYGG	5 cut(s)	96, 1142, 1427, 1712, 1997
BstENI	CCTNNNNNAGG	1 cut(s)	3443
BstF5I	GGATG	8 cut(s)	742, 944, 961, 1229, 1514, 1799, 2209, 3532
BstHHI	GCGC	3 cut(s)	150, 463, 3556
BstMWI	GCNNNNNNNGC	4 cut(s)	1224, 1509, 1794, 3553
BstNI	CCWGG	4 cut(s)	641, 1590, 2375, 2523
BstNSI	RCATGY	5 cut(s)	543, 732, 830, 912, 1887
BstSCI	CCNGG	7 cut(s)	177, 178, 639, 1303, 1588, 2373, 2521
BstSFI	CTRYAG	1 cut(s)	561
BstSLI	GKGCMC	2 cut(s)	3296, 3549
BstSNI	TACGTA	1 cut(s)	89
BstV1I	GCAGC	8 cut(s)	442, 445, 1114, 1399, 1684, 1969, 3168, 3617
BstV2I	GAAGAC	4 cut(s)	563, 716, 2732, 3735
BstX2I	RGATCY	8 cut(s)	435, 521, 797, 2093, 2236, 2466, 2628, 3219
BstYI	RGATCY	8 cut(s)	435, 521, 797, 2093, 2236, 2466, 2628, 3219
BstZ17I	GTATAC	4 cut(s)	1000, 1285, 1570, 1855
Bsu15I	ATCGAT	1 cut(s)	678
Bsu36I	CCTNAGG	1 cut(s)	2471
BsuI	GTATCC	1 cut(s)	2284
BsuRI	GGCC	3 cut(s)	230, 2577, 2706
BsuTUI	ATCGAT	1 cut(s)	678
BtgI	CCRYGG	5 cut(s)	96, 1142, 1427, 1712, 1997
BtrI	CACGTC	1 cut(s)	302
BtsCI	GGATG	8 cut(s)	742, 944, 961, 1229, 1514, 1799, 2209, 3532
BtsI	GCAGTG	1 cut(s)	17
BtsIMutI	CAGTG	3 cut(s)	17, 711, 1965
Cac8I	GCNNGC	1 cut(s)	3851
CaiI	CAGNNNCTG	1 cut(s)	3182
CfoI	GCGC	3 cut(s)	150, 463, 3556
Cfr13I	GGNCC	2 cut(s)	215, 3278
Cfr9I	CCCGGG	1 cut(s)	178
ClaI	ATCGAT	1 cut(s)	678
CseI	GACGC	3 cut(s)	1119, 1404, 1689
DdeI	CTNAG	9 cut(s)	72, 135, 406, 2122, 2471, 2592, 2833, 3175, 3183
DraI	TTTAAA	2 cut(s)	2463, 3355
DraIII	CACNNNGTG	1 cut(s)	2350
DrdI	GACNNNNNNGTC	1 cut(s)	195
DseDI	GACNNNNNNGTC	1 cut(s)	195
Eam1104I	CTCTTC	4 cut(s)	744, 1574, 2400, 3438
EarI	CTCTTC	4 cut(s)	744, 1574, 2400, 3438
Ecl136II	GAGCTC	1 cut(s)	3436
Eco105I	TACGTA	1 cut(s)	89
Eco130I	CCWWGG	8 cut(s)	96, 1142, 1228, 1427, 1513, 1712, 1798, 2707
Eco24I	GRGCYC	1 cut(s)	3438
Eco31I	GGTCTC	6 cut(s)	1453, 1738, 2023, 2910, 2943, 3014
Eco47I	GGWCC	2 cut(s)	215, 3278
Eco53kI	GAGCTC	1 cut(s)	3436
Eco57I	CTGAAG	6 cut(s)	36, 1935, 2763, 3387, 3477, 3747
Eco81I	CCTNAGG	1 cut(s)	2471
Eco88I	CYCGRG	2 cut(s)	178, 2219
EcoICRI	GAGCTC	1 cut(s)	3436
EcoNI	CCTNNNNNAGG	1 cut(s)	3443
EcoO109I	RGGNCCY	1 cut(s)	215
EcoRI	GAATTC	1 cut(s)	3699
EcoRII	CCWGG	4 cut(s)	639, 1588, 2373, 2521
EcoT14I	CCWWGG	8 cut(s)	96, 1142, 1228, 1427, 1513, 1712, 1798, 2707
EcoT22I	ATGCAT	1 cut(s)	914
EcoT38I	GRGCYC	1 cut(s)	3438
ErhI	CCWWGG	8 cut(s)	96, 1142, 1228, 1427, 1513, 1712, 1798, 2707
FaqI	GGGAC	3 cut(s)	228, 1282, 2504
FblI	GTMKAC	5 cut(s)	943, 999, 1284, 1569, 1854
FokI	GGATG	8 cut(s)	749, 951, 968, 1236, 1521, 1806, 2216, 3539
FriOI	GRGCYC	1 cut(s)	3438
FspBI	CTAG	7 cut(s)	39, 2097, 2295, 2307, 2640, 3032, 3108
GlaI	GCGC	3 cut(s)	149, 462, 3555
GsaI	CCCAGC	4 cut(s)	1118, 1403, 1688, 1973
GsuI	CTGGAG	1 cut(s)	572
HaeIII	GGCC	3 cut(s)	230, 2577, 2706
HapII	CCGG	2 cut(s)	179, 1304
HgaI	GACGC	3 cut(s)	1119, 1404, 1689
HhaI	GCGC	3 cut(s)	150, 463, 3556
Hin6I	GCGC	3 cut(s)	148, 461, 3554
HinP1I	GCGC	3 cut(s)	148, 461, 3554
HincII	GTYRAC	5 cut(s)	2109, 2119, 2338, 3323, 3753
HindII	GTYRAC	5 cut(s)	2109, 2119, 2338, 3323, 3753
HindIII	AAGCTT	2 cut(s)	2753, 2915
HpaII	CCGG	2 cut(s)	179, 1304
Hpy99I	CGWCG	1 cut(s)	3239
HpyCH4III	ACNGT	9 cut(s)	349, 388, 706, 1424, 1960, 2104, 2350, 3239, 3842
HpyCH4IV	ACGT	6 cut(s)	88, 301, 882, 1375, 2852, 2887
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1224, 1509, 1794, 3553
HpyF3I	CTNAG	9 cut(s)	72, 135, 406, 2122, 2471, 2592, 2833, 3175, 3183
HpySE526I	ACGT	6 cut(s)	88, 301, 882, 1375, 2852, 2887
HspAI	GCGC	3 cut(s)	148, 461, 3554
LmnI	GCTCC	2 cut(s)	2513, 3441
Lsp1109I	GCAGC	8 cut(s)	442, 445, 1114, 1399, 1684, 1969, 3168, 3617
MaeI	CTAG	7 cut(s)	39, 2097, 2295, 2307, 2640, 3032, 3108
MaeII	ACGT	6 cut(s)	88, 301, 882, 1375, 2852, 2887
MaeIII	GTNAC	8 cut(s)	129, 293, 362, 605, 932, 2245, 3103, 3305
MfeI	CAATTG	5 cut(s)	1058, 1343, 1628, 1913, 2331
MflI	RGATCY	8 cut(s)	435, 521, 797, 2093, 2236, 2466, 2628, 3219
MhlI	GDGCHC	4 cut(s)	116, 3296, 3438, 3549
MlyI	GAGTC	4 cut(s)	1864, 2114, 2242, 2584
MmeI	TCCRAC	4 cut(s)	463, 1510, 1795, 3113
Mph1103I	ATGCAT	1 cut(s)	914
MroXI	GAANNNNTTC	4 cut(s)	49, 634, 753, 3732
MseI	TTAA	9 cut(s)	57, 140, 203, 398, 567, 2462, 3044, 3354, 3636
MslI	CAYNNNNRTG	1 cut(s)	762
MspA1I	CMGCKG	3 cut(s)	161, 3159, 3725
MspI	CCGG	2 cut(s)	179, 1304
MspR9I	CCNGG	7 cut(s)	179, 180, 641, 1305, 1590, 2375, 2523
MunI	CAATTG	5 cut(s)	1058, 1343, 1628, 1913, 2331
Mva1269I	GAATGC	2 cut(s)	964, 3176
MvaI	CCWGG	4 cut(s)	641, 1590, 2375, 2523
MwoI	GCNNNNNNNGC	4 cut(s)	1224, 1509, 1794, 3553
NciI	CCSGG	3 cut(s)	179, 180, 1305
NcoI	CCATGG	4 cut(s)	96, 1142, 1427, 1712
NlaIV	GGNNCC	6 cut(s)	216, 799, 1089, 2739, 2801, 3131
NmuCI	GTSAC	4 cut(s)	129, 293, 605, 2245
NsiI	ATGCAT	1 cut(s)	914
NspI	RCATGY	5 cut(s)	543, 732, 830, 912, 1887
NspV	TTCGAA	1 cut(s)	872
OliI	CACNNNNGTG	1 cut(s)	762
PaeR7I	CTCGAG	1 cut(s)	2219
PciI	ACATGT	2 cut(s)	728, 1883
PcsI	WCGNNNNNNNCGW	1 cut(s)	2164
PctI	GAATGC	2 cut(s)	964, 3176
PdmI	GAANNNNTTC	4 cut(s)	49, 634, 753, 3732
Pfl23II	CGTACG	1 cut(s)	122
PflMI	CCANNNNNTGG	4 cut(s)	1234, 1469, 1754, 1804
PleI	GAGTC	4 cut(s)	1864, 2114, 2242, 2584
PpsI	GAGTC	4 cut(s)	1864, 2114, 2242, 2584
Ppu21I	YACGTR	1 cut(s)	89
PpuMI	RGGWCCY	1 cut(s)	215
PscI	ACATGT	2 cut(s)	728, 1883
PsiI	TTATAA	2 cut(s)	1050, 1905
Psp124BI	GAGCTC	1 cut(s)	3438
Psp1406I	AACGTT	1 cut(s)	2887
Psp5II	RGGWCCY	1 cut(s)	215
Psp6I	CCWGG	4 cut(s)	639, 1588, 2373, 2521
PspFI	CCCAGC	4 cut(s)	1114, 1399, 1684, 1969
PspGI	CCWGG	4 cut(s)	639, 1588, 2373, 2521
PspLI	CGTACG	1 cut(s)	122
PspN4I	GGNNCC	6 cut(s)	216, 799, 1089, 2739, 2801, 3131
PspPI	GGNCC	2 cut(s)	215, 3278
PspPPI	RGGWCCY	1 cut(s)	215
PstI	CTGCAG	1 cut(s)	565
PstNI	CAGNNNCTG	1 cut(s)	3182
PsuI	RGATCY	8 cut(s)	435, 521, 797, 2093, 2236, 2466, 2628, 3219
PvuII	CAGCTG	1 cut(s)	3159
RseI	CAYNNNNRTG	1 cut(s)	762
SacI	GAGCTC	1 cut(s)	3438
SaqAI	TTAA	9 cut(s)	57, 140, 203, 398, 567, 2462, 3044, 3354, 3636
Sau96I	GGNCC	2 cut(s)	215, 3278
ScaI	AGTACT	2 cut(s)	376, 2101
SchI	GAGTC	4 cut(s)	1864, 2114, 2242, 2584
ScrFI	CCNGG	7 cut(s)	179, 180, 641, 1305, 1590, 2375, 2523
SduI	GDGCHC	4 cut(s)	116, 3296, 3438, 3549
SfcI	CTRYAG	1 cut(s)	561
Sfr274I	CTCGAG	1 cut(s)	2219
SfuI	TTCGAA	1 cut(s)	872
SinI	GGWCC	2 cut(s)	215, 3278
SlaI	CTCGAG	1 cut(s)	2219
SmaI	CCCGGG	1 cut(s)	180
SmiMI	CAYNNNNRTG	1 cut(s)	762
SmlI	CTYRAG	4 cut(s)	1494, 1779, 2219, 3146
SmoI	CTYRAG	4 cut(s)	1494, 1779, 2219, 3146
SnaBI	TACGTA	1 cut(s)	89
SpeI	ACTAGT	3 cut(s)	2306, 2639, 3107
SsiI	CCGC	4 cut(s)	159, 231, 1185, 3723
SspI	AATATT	2 cut(s)	26, 502
SspMI	CTAG	7 cut(s)	39, 2097, 2295, 2307, 2640, 3032, 3108
SstI	GAGCTC	1 cut(s)	3438
StyD4I	CCNGG	7 cut(s)	177, 178, 639, 1303, 1588, 2373, 2521
StyI	CCWWGG	8 cut(s)	96, 1142, 1228, 1427, 1513, 1712, 1798, 2707
TaaI	ACNGT	9 cut(s)	349, 388, 706, 1424, 1960, 2104, 2350, 3239, 3842
TaiI	ACGT	6 cut(s)	91, 304, 885, 1378, 2855, 2890
TaqI	TCGA	7 cut(s)	583, 678, 872, 1097, 2220, 2947, 3234
TaqII	GACCGA	1 cut(s)	728
TatI	WGTACW	5 cut(s)	374, 832, 2099, 3842, 3855
TauI	GCSGC	2 cut(s)	233, 3725
Tru1I	TTAA	9 cut(s)	57, 140, 203, 398, 567, 2462, 3044, 3354, 3636
Tru9I	TTAA	9 cut(s)	57, 140, 203, 398, 567, 2462, 3044, 3354, 3636
TscAI	CASTG	3 cut(s)	24, 711, 1965
TseFI	GTSAC	4 cut(s)	129, 293, 605, 2245
TseI	GCWGC	8 cut(s)	455, 458, 1127, 1412, 1697, 1982, 3156, 3605
Tsp45I	GTSAC	4 cut(s)	129, 293, 605, 2245
TspDTI	ATGAA	9 cut(s)	429, 762, 870, 2231, 2537, 2570, 2738, 2808, 3543
TspGWI	ACGGA	7 cut(s)	110, 312, 951, 1286, 1986, 3225, 3288
TspMI	CCCGGG	1 cut(s)	178
TspRI	CASTG	3 cut(s)	24, 711, 1965
Van91I	CCANNNNNTGG	4 cut(s)	1234, 1469, 1754, 1804
VpaK11BI	GGWCC	2 cut(s)	215, 3278
XagI	CCTNNNNNAGG	1 cut(s)	3443
XbaI	TCTAGA	1 cut(s)	38
XceI	RCATGY	5 cut(s)	543, 732, 830, 912, 1887
XcmI	CCANNNNNNNNNTGG	1 cut(s)	446
XhoI	CTCGAG	1 cut(s)	2219
XmaI	CCCGGG	1 cut(s)	178
XmiI	GTMKAC	5 cut(s)	943, 999, 1284, 1569, 1854
XmnI	GAANNNNTTC	4 cut(s)	49, 634, 753, 3732
XspI	CTAG	7 cut(s)	39, 2097, 2295, 2307, 2640, 3032, 3108
ZrmI	AGTACT	2 cut(s)	376, 2101
Zsp2I	ATGCAT	1 cut(s)	914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.