Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000133

Physical Location & Seq

Genomic Coordinates Reverse (-)

3436991 .. 3439517

Gene Structure 2527 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1050400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGAAGATTACTTCTTATTGATCAAAATGTTGGTATTTTCTTTGGTTTCTGTAATTCTGTATTTGGTTCTGAGAGTTGTTCATGTCTATTGGGTAAGACCCAGAAGCTTAGAGAAGCAGTTGAGAAAGCAAGGCATCAGAGGCAGATCTTACAAGCTTTTCCATGATGACATGAAAGAAATGAGCACGTCCAGCAGAGAAGCATGGTCCAAACCCATGTCACTCAACCACCAGATTGCTCCACGTGTCCTCCCATATTTTCATCAAATGGTTCAAAAATATGGAAAAGTGAGTTTGGGGTGGATGGAAACAAGGCCAAGGCTAATAGTAACAGACCCAGAGCTAATCAAGGATATATTAATAAACAAAAATGGGCATTTCATAATGCCACCACTGAATCCACTTGTGAAACTTCTGCAATTGGGCGTCTCAACCTTGGAAGGAGAGCAATGGACCAAACGCAGAAGGATCATCAATCCTGCTTTCCACCTTGAGAAATTAATGGGTATGGAGACTTCATTTGTAACCGGTTGTTCTGGCATGATCGATCGATGGGAAAAGTTAATTGGTGTTGAAGGATCAAGGGAAGTAGATGTGGCACCAGAATTTCAAAATTTGATTAGTGAAGTTATAGCTCTAACGGCCTTTGGAGGCAGCTTTGAAGATGGGAAGCTATTTGAGCTTCAGAAAAAGCAAGCCGCTTTATCACGTGAAGCCTATTATGGTTTCTATTTCCCAGGGTTCAGTTTTTGTTCTCTGCGCAGATTCATACCCACCAAAAAGAACAGATTGAGGTATAAGGTGGACAATGAAATCAAAGCAATATTAAGGGCTACGATCAGGAAGAAAGAGCAAGCCATGGAAAATGGTGAAGTGGGTGCAAATAATGATTTGTTGGGTTTACTGTTACAATGCAAAGCACAAGAGGAAAATAGTATGACAATTGAGGATGTCATAGAGGAGTGCAAGTCATTCTACTTTGCTGGCCAAGAAAGCATAGCCACCTTGTTGACTTGGACGATGATTCTGTTATGCATGCATCCAAACTGGCAAGAAAAGGCAAGAGAAGAAGTCCTCCGTGTGTTTGGAAAGAAAACACCTGATTTAGATGGCATAAAACACCTCAAAATCGTGACGATGATATTGACCGAAGTTTTGAGGCTATATCCATCTCTAGTTCTTCTATTTAGGCACACCCACCAGAAAACAAATGTAGGAGGCCTTTCCATCCCAGCTGGGGTTGATTTCGTGTTTCCGATTCTTTTTCTACATCACGATCCAAAATATTGGGGTGAAGATGTTGAGGAGTTCAACCCAGAGAGATTTTCCGAAGGAGTTGTGAAGGCATCAAAGGATCAAACTGCATTTTTCTCATTTGGGTGGGGGCCTAGATTATGTCTAGGGCAAACTTTTGCTATGTTAGAAGGAAAGGTAGCTCTGGCGATGATTCTTCAGCATTTTTCACTTGAGCTCTCACCTTCTTACACTCATGCTCCTGTTATCTCGATTTCCGTTAAGCCAAAACATGGAGCCCCAATCATACTGCAGAGGATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0006629 GO:0008150 GO:0008152 GO:0008202 GO:0008395 GO:0009719 GO:0009725 GO:0009741 GO:0010033 GO:0010268 GO:0010817 GO:0014070 GO:0016128 GO:0016131 GO:0016491 GO:0033993 GO:0042221 GO:0042445 GO:0042592 GO:0044238 GO:0048878 GO:0050896 GO:0055088 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901360 GO:1901615 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00908 ko01100 ko01110 ko04726 map00908 map01100 map01110 map04726

Pfam Domains

Protein Families

p450

Protein Analysis

519

Amino Acids

59.5

Weight (kDa)

9.1

Isoelectric Point (pI)

42.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	86 - 494	7.3e-81	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14610 AT3G14620 AT3G14630 AT3G14630 AT3G14640 AT3G14640 AT3G14650 AT3G14660 AT3G14660 AT3G14660 AT3G14680 AT3G14690 AT3G14690
fragaria_vesca	FvH4_1g08410 FvH4_2g31040 FvH4_3g17200 FvH4_3g17200 FvH4_3g17220 FvH4_3g17220 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17260 FvH4_3g17260 FvH4_3g17263 FvH4_3g17264 FvH4_3g17265 FvH4_3g17266 FvH4_5g18530 FvH4_5g19690 FvH4_7g03990 FvH4_7g03990 FvH4_7g03990 FvH4_7g03990 FvH4_7g03990 FvH4_7g17900 FvH4_7g17900
malus_domestica	MD02G1088200.v1.1 MD03G1224800.v1.1 MD03G1224900.v1.1 MD03G1225000.v1.1 MD11G1274100.v1.1 MD11G1274200.v1.1 MD11G1274300.v1.1 MD11G1274500.v1.1 MD11G1274600.v1.1 MD15G1050400.v1.1 MD15G1050500.v1.1 MD15G1050600.v1.1 MD15G1050700.v1.1
prunus_persica	Prupe.1G404900_v2.0.a1 Prupe.1G404900_v2.0.a1 Prupe.4G152600_v2.0.a1 Prupe.4G152700_v2.0.a1 Prupe.4G152800_v2.0.a1 Prupe.4G152900_v2.0.a1 Prupe.4G153000_v2.0.a1 Prupe.7G201400_v2.0.a1 Prupe.7G201400_v2.0.a1
pyrus_communis	pycom03g17310 pycom03g17330 pycom11g21520 pycom11g21600 pycom11g24180 pycom11g24190 pycom11g24200 pycom11g24210 pycom15g04890
rosa_chinensis	RchiOBHm_Chr1g0327671 RchiOBHm_Chr1g0327711 RchiOBHm_Chr1g0327721 RchiOBHm_Chr2g0094501 RchiOBHm_Chr5g0028601 RchiOBHm_Chr5g0028611 RchiOBHm_Chr5g0028621 RchiOBHm_Chr5g0028661 RchiOBHm_Chr5g0028671 RchiOBHm_Chr5g0028701 RchiOBHm_Chr5g0028721 RchiOBHm_Chr5g0028741 RchiOBHm_Chr5g0028751 RchiOBHm_Chr5g0028771 RchiOBHm_Chr5g0028821 RchiOBHm_Chr6g0311761 RchiOBHm_Chr7g0204421
rosa_laevigata	RLG00000003223 RLG00000003462 RLG00000010376 RLG00000012452 RLG00000016496 RLG00000018531 RLG00000018532 RLG00000018536 RLG00000029973 RLG00000029976 RLG00000033105 RLG00000033106 RLG00000033107 RLG00000033109 RLG00000033110 RLG00000033111 RLG00000033112 RLG00000033113 RLG00000033114 RLG00000033116 RLG00000033124
rosa_multiflora	Rmu_co8029584.1_g000001 Rmu_co8159878.1_g000001 Rmu_co8310611.1_g000001 Rmu_sc0001000.1_g000004 Rmu_sc0001000.1_g000016 Rmu_sc0001575.1_g000020 Rmu_sc0001575.1_g000021 Rmu_sc0001575.1_g000022 Rmu_sc0001575.1_g000027 Rmu_sc0001575.1_g000035 Rmu_sc0001575.1_g000040 Rmu_sc0002888.1_g000039 Rmu_sc0003951.1_g000001 Rmu_sc0004359.1_g000045 Rmu_sc0005178.1_g000001 Rmu_sc0005178.1_g000002 Rmu_sc0005178.1_g000004 Rmu_sc0023855.1_g000001 Rmu_sc0024393.1_g000001 Rmu_sc0035326.1_g000001
rosa_roxburghii	Rroxscaffold_1G00051080 Rroxscaffold_1G00051100 Rroxscaffold_1G00051110 Rroxscaffold_1G00051120 Rroxscaffold_1G00051150 Rroxscaffold_1G00051160 Rroxscaffold_1G00051170 Rroxscaffold_1G00051180 Rroxscaffold_2G00123610 Rroxscaffold_2G00147120 Rroxscaffold_3G00250540 Rroxscaffold_3G00253110 Rroxscaffold_3G00253120 Rroxscaffold_4G00322410 Rroxscaffold_4G00322440 Rroxscaffold_4G00322470 Rroxscaffold_7G00157330
rosa_rugosa	Rorug01G0069500 Rorug01G0069600 Rorug01G0069700 Rorug02G0044200 Rorug02G0227700 Rorug05G0109800 Rorug05G0109900 Rorug05G0110000 Rorug05G0110100 Rorug05G0110300 Rorug05G0110400 Rorug05G0110500 Rorug07G0083800 Rorug07G0083900.1 Rorug07G0103700
rosa_samantha	Rh1BG069000 Rh1BG069100 Rh1BG069200 Rh1CG084000 Rh1DG090700 Rh1DG090800 Rh1DG090900 Rh2AG092500 Rh2DG091600 Rh2DG309400 Rh5AG202100 Rh5AG202200 Rh5AG202500 Rh5AG202800 Rh5AG202900 Rh5CG220600 Rh5CG220700 Rh5CG220800 Rh5CG220900 Rh5CG221600 Rh5CG221900 Rh5CG222000 Rh5CG222100 Rh5DG203300 Rh5DG203400 Rh5DG203500 Rh5DG204100 Rh5DG204300 Rh5DG204400 Rh5DG204600 Rh6BG517600 Rh6CG523400 Rh6DG509100 Rh7AG213600 Rh7BG211400 Rh7DG220800
rosa_wichuraiana	Rw0G010630 Rw1G006750 Rw1G006770 Rw5G018300 Rw5G018310 Rw5G018330 Rw6G044090 Rw7G018510 Rw7G020290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	761
AccB1I	GGYRCC	1 cut(s)	598
AccB7I	CCANNNNNTGG	1 cut(s)	1526
AciI	CCGC	1 cut(s)	699
AclWI	GGATC	4 cut(s)	476, 586, 1271, 1362
AcoI	YGGCCR	1 cut(s)	985
AcsI	RAATTY	2 cut(s)	605, 613
AcuI	CTGAAG	2 cut(s)	668, 1436
AcvI	CACGTG	2 cut(s)	245, 710
AcyI	GRCGYC	1 cut(s)	426
AfiI	CCNNNNNNNGG	2 cut(s)	1237, 1526
AflIII	ACRYGT	1 cut(s)	244
AgeI	ACCGGT	1 cut(s)	527
AgsI	TTSAA	5 cut(s)	275, 575, 611, 662, 1312
AjiI	CACGTC	1 cut(s)	189
AjnI	CCWGG	1 cut(s)	736
AjuI	GAANNNNNNNTTGG	2 cut(s)	549, 581
Alw21I	GWGCWC	2 cut(s)	188, 1473
Alw26I	GTCTC	2 cut(s)	433, 506
AlwI	GGATC	4 cut(s)	476, 586, 1271, 1362
AoxI	GGCC	5 cut(s)	314, 642, 985, 1219, 1385
ApeKI	GCWGC	1 cut(s)	654
ApoI	RAATTY	2 cut(s)	605, 613
ArsI	GACNNNNNNTTYG	2 cut(s)	1138, 1170
AseI	ATTAAT	2 cut(s)	359, 500
AsiGI	ACCGGT	1 cut(s)	527
AspLEI	GCGC	1 cut(s)	762
AspS9I	GGNCC	3 cut(s)	207, 453, 1385
AsuHPI	GGTGA	3 cut(s)	881, 1304, 1467
AvaII	GGWCC	2 cut(s)	207, 453
BalI	TGGCCA	1 cut(s)	987
BanI	GGYRCC	1 cut(s)	598
BanII	GRGCYC	2 cut(s)	1473, 1534
BarI	GAAGNNNNNNTAC	2 cut(s)	499, 531
BbrPI	CACGTG	2 cut(s)	245, 710
Bbv12I	GWGCWC	2 cut(s)	188, 1473
BbvI	GCAGC	1 cut(s)	666
BccI	CCATC	6 cut(s)	298, 546, 659, 1103, 1177, 1235
BceAI	ACGGC	1 cut(s)	657
BciT130I	CCWGG	1 cut(s)	738
BclI	TGATCA	1 cut(s)	21
BcoDI	GTCTC	2 cut(s)	433, 506
BfaI	CTAG	3 cut(s)	1175, 1389, 1400
BfmI	CTRYAG	1 cut(s)	1544
BglII	AGATCT	1 cut(s)	146
BisI	GCNGC	2 cut(s)	655, 699
BlsI	GCNGC	2 cut(s)	656, 700
Bme1390I	CCNGG	1 cut(s)	738
Bme18I	GGWCC	2 cut(s)	207, 453
BmgBI	CACGTC	1 cut(s)	189
BmgT120I	GGNCC	3 cut(s)	207, 453, 1385
BmiI	GGNNCC	3 cut(s)	600, 1386, 1531
BmrFI	CCNGG	1 cut(s)	738
BmsI	GCATC	3 cut(s)	144, 1048, 1355
BpuEI	CTTGAG	2 cut(s)	512, 1487
Bsa29I	ATCGAT	2 cut(s)	546, 550
BsaAI	YACGTR	2 cut(s)	245, 710
BsaHI	GRCGYC	1 cut(s)	426
BsaJI	CCNNGG	5 cut(s)	317, 435, 736, 737, 858
BsaWI	WCCGGW	1 cut(s)	527
BsaXI	ACNNNNNCTCC	6 cut(s)	234, 264, 953, 983, 1477, 1507
Bsc4I	CCNNNNNNNGG	2 cut(s)	1237, 1526
Bse118I	RCCGGY	1 cut(s)	527
Bse1I	ACTGG	1 cut(s)	1052
Bse3DI	GCAATG	1 cut(s)	455
BseBI	CCWGG	1 cut(s)	738
BseCI	ATCGAT	2 cut(s)	546, 550
BseDI	CCNNGG	5 cut(s)	317, 435, 736, 737, 858
BseGI	GGATG	4 cut(s)	309, 955, 1039, 1227
BseLI	CCNNNNNNNGG	2 cut(s)	1237, 1526
BseMI	GCAATG	1 cut(s)	455
BseMII	CTCAG	1 cut(s)	62
BseNI	ACTGG	1 cut(s)	1052
BseRI	GAGGAG	2 cut(s)	974, 1319
BseXI	GCAGC	1 cut(s)	666
BseYI	CCCAGC	2 cut(s)	1231, 1235
Bsh1285I	CGRYCG	1 cut(s)	550
BshFI	GGCC	5 cut(s)	316, 644, 987, 1221, 1387
BshNI	GGYRCC	1 cut(s)	598
BshTI	ACCGGT	1 cut(s)	527
BshVI	ATCGAT	2 cut(s)	546, 550
BsiEI	CGRYCG	1 cut(s)	550
BsiHKAI	GWGCWC	2 cut(s)	188, 1473
BsiSI	CCGG	1 cut(s)	528
BslI	CCNNNNNNNGG	2 cut(s)	1237, 1526
BsmAI	GTCTC	2 cut(s)	433, 506
BsmBI	CGTCTC	1 cut(s)	433
BsnI	GGCC	5 cut(s)	316, 644, 987, 1221, 1387
Bsp1286I	GDGCHC	3 cut(s)	188, 1473, 1534
Bsp143I	GATC	9 cut(s)	21, 146, 468, 543, 547, 578, 837, 1276, 1354
Bsp19I	CCATGG	1 cut(s)	858
BspACI	CCGC	1 cut(s)	699
BspANI	GGCC	5 cut(s)	316, 644, 987, 1221, 1387
BspCNI	CTCAG	1 cut(s)	63
BspDI	ATCGAT	2 cut(s)	546, 550
BspLI	GGNNCC	3 cut(s)	600, 1386, 1531
BspMAI	CTGCAG	1 cut(s)	1548
BspPI	GGATC	4 cut(s)	476, 586, 1271, 1362
BspT107I	GGYRCC	1 cut(s)	598
BsrDI	GCAATG	1 cut(s)	455
BsrFI	RCCGGY	1 cut(s)	527
BsrI	ACTGG	1 cut(s)	1052
BssAI	RCCGGY	1 cut(s)	527
BssECI	CCNNGG	5 cut(s)	317, 435, 736, 737, 858
BssMI	GATC	9 cut(s)	21, 146, 468, 543, 547, 578, 837, 1276, 1354
BssNI	GRCGYC	1 cut(s)	426
BssT1I	CCWWGG	3 cut(s)	317, 435, 858
Bst2UI	CCWGG	1 cut(s)	738
Bst4CI	ACNGT	1 cut(s)	906
BstACI	GRCGYC	1 cut(s)	426
BstBAI	YACGTR	2 cut(s)	245, 710
BstC8I	GCNNGC	4 cut(s)	696, 855, 985, 1037
BstDEI	CTNAG	2 cut(s)	71, 109
BstDSI	CCRYGG	1 cut(s)	858
BstF5I	GGATG	4 cut(s)	309, 955, 1039, 1227
BstHHI	GCGC	1 cut(s)	762
BstKTI	GATC	9 cut(s)	24, 149, 471, 546, 550, 581, 840, 1279, 1357
BstMAI	GTCTC	2 cut(s)	433, 506
BstMBI	GATC	9 cut(s)	21, 146, 468, 543, 547, 578, 837, 1276, 1354
BstMCI	CGRYCG	1 cut(s)	550
BstMWI	GCNNNNNNNGC	5 cut(s)	141, 192, 641, 679, 993
BstNI	CCWGG	1 cut(s)	738
BstNSI	RCATGY	1 cut(s)	1039
BstSCI	CCNGG	1 cut(s)	736
BstSFI	CTRYAG	1 cut(s)	1544
BstV1I	GCAGC	1 cut(s)	666
BstX2I	RGATCY	1 cut(s)	146
BstXI	CCANNNNNNTGG	1 cut(s)	1287
BstYI	RGATCY	1 cut(s)	146
Bsu15I	ATCGAT	2 cut(s)	546, 550
BsuRI	GGCC	5 cut(s)	316, 644, 987, 1221, 1387
BsuTUI	ATCGAT	2 cut(s)	546, 550
BtgI	CCRYGG	1 cut(s)	858
BtgZI	GCGATG	1 cut(s)	1457
BtrI	CACGTC	1 cut(s)	189
BtsCI	GGATG	4 cut(s)	309, 955, 1039, 1227
BtsIMutI	CAGTG	1 cut(s)	392
Cac8I	GCNNGC	4 cut(s)	696, 855, 985, 1037
CfoI	GCGC	1 cut(s)	762
Cfr10I	RCCGGY	1 cut(s)	527
Cfr13I	GGNCC	3 cut(s)	207, 453, 1385
ClaI	ATCGAT	2 cut(s)	546, 550
CseI	GACGC	1 cut(s)	415
CspAI	ACCGGT	1 cut(s)	527
DdeI	CTNAG	2 cut(s)	71, 109
DpnI	GATC	9 cut(s)	23, 148, 470, 545, 549, 580, 839, 1278, 1356
DpnII	GATC	9 cut(s)	21, 146, 468, 543, 547, 578, 837, 1276, 1354
EaeI	YGGCCR	1 cut(s)	985
Ecl136II	GAGCTC	1 cut(s)	1471
Eco130I	CCWWGG	3 cut(s)	317, 435, 858
Eco147I	AGGCCT	1 cut(s)	1221
Eco24I	GRGCYC	2 cut(s)	1473, 1534
Eco47I	GGWCC	2 cut(s)	207, 453
Eco53kI	GAGCTC	1 cut(s)	1471
Eco57I	CTGAAG	2 cut(s)	668, 1436
Eco72I	CACGTG	2 cut(s)	245, 710
EcoICRI	GAGCTC	1 cut(s)	1471
EcoO109I	RGGNCCY	1 cut(s)	1385
EcoRII	CCWGG	1 cut(s)	736
EcoT14I	CCWWGG	3 cut(s)	317, 435, 858
EcoT22I	ATGCAT	2 cut(s)	1037, 1041
EcoT38I	GRGCYC	2 cut(s)	1473, 1534
ErhI	CCWWGG	3 cut(s)	317, 435, 858
Esp3I	CGTCTC	1 cut(s)	433
FbaI	TGATCA	1 cut(s)	21
Fnu4HI	GCNGC	2 cut(s)	655, 699
FokI	GGATG	4 cut(s)	316, 962, 1026, 1214
FriOI	GRGCYC	2 cut(s)	1473, 1534
Fsp4HI	GCNGC	2 cut(s)	655, 699
FspBI	CTAG	3 cut(s)	1175, 1389, 1400
FspI	TGCGCA	1 cut(s)	761
GlaI	GCGC	1 cut(s)	761
GluI	GCNGC	2 cut(s)	655, 699
GsaI	CCCAGC	2 cut(s)	1235, 1239
HaeIII	GGCC	5 cut(s)	316, 644, 987, 1221, 1387
HapII	CCGG	1 cut(s)	528
HgaI	GACGC	1 cut(s)	415
HhaI	GCGC	1 cut(s)	762
Hin1I	GRCGYC	1 cut(s)	426
Hin6I	GCGC	1 cut(s)	760
HinP1I	GCGC	1 cut(s)	760
HincII	GTYRAC	1 cut(s)	1011
HindII	GTYRAC	1 cut(s)	1011
HindIII	AAGCTT	2 cut(s)	106, 155
HinfI	GANTC	5 cut(s)	397, 765, 1024, 1258, 1447
HpaII	CCGG	1 cut(s)	528
HphI	GGTGA	3 cut(s)	881, 1304, 1467
Hpy166II	GTNNAC	3 cut(s)	805, 902, 1011
Hpy188I	TCNGA	5 cut(s)	72, 140, 687, 1257, 1330
Hpy188III	TCNNGA	4 cut(s)	841, 1132, 1274, 1504
Hpy8I	GTNNAC	3 cut(s)	805, 902, 1011
HpyAV	CCTTC	7 cut(s)	434, 459, 569, 1325, 1336, 1418, 1488
HpyCH4III	ACNGT	1 cut(s)	906
HpyCH4IV	ACGT	3 cut(s)	188, 244, 709
HpyCH4V	TGCA	8 cut(s)	418, 881, 915, 966, 1035, 1039, 1364, 1546
HpyF10VI	GCNNNNNNNGC	5 cut(s)	141, 192, 641, 679, 993
HpyF3I	CTNAG	2 cut(s)	71, 109
HpySE526I	ACGT	3 cut(s)	188, 244, 709
Hsp92I	GRCGYC	1 cut(s)	426
HspAI	GCGC	1 cut(s)	760
Ksp22I	TGATCA	1 cut(s)	21
Kzo9I	GATC	9 cut(s)	21, 146, 468, 543, 547, 578, 837, 1276, 1354
LmnI	GCTCC	3 cut(s)	244, 1498, 1529
Lsp1109I	GCAGC	1 cut(s)	666
LweI	GCATC	3 cut(s)	144, 1048, 1355
MaeI	CTAG	3 cut(s)	1175, 1389, 1400
MaeII	ACGT	3 cut(s)	188, 244, 709
MaeIII	GTNAC	5 cut(s)	219, 328, 523, 906, 1132
MalI	GATC	9 cut(s)	23, 148, 470, 545, 549, 580, 839, 1278, 1356
MboI	GATC	9 cut(s)	21, 146, 468, 543, 547, 578, 837, 1276, 1354
MboII	GAAGA	7 cut(s)	17, 674, 856, 1079, 1172, 1307, 1442
MfeI	CAATTG	2 cut(s)	419, 942
MflI	RGATCY	1 cut(s)	146
MhlI	GDGCHC	3 cut(s)	188, 1473, 1534
MlsI	TGGCCA	1 cut(s)	987
MluCI	AATT	7 cut(s)	54, 419, 497, 564, 605, 613, 942
MluNI	TGGCCA	1 cut(s)	987
Mox20I	TGGCCA	1 cut(s)	987
Mph1103I	ATGCAT	2 cut(s)	1037, 1041
MscI	TGGCCA	1 cut(s)	987
MseI	TTAA	5 cut(s)	359, 500, 563, 827, 1515
MslI	CAYNNNNRTG	1 cut(s)	1378
Msp20I	TGGCCA	1 cut(s)	987
MspA1I	CMGCKG	1 cut(s)	1235
MspI	CCGG	1 cut(s)	528
MspR9I	CCNGG	1 cut(s)	738
MunI	CAATTG	2 cut(s)	419, 942
MvaI	CCWGG	1 cut(s)	738
MwoI	GCNNNNNNNGC	5 cut(s)	141, 192, 641, 679, 993
NcoI	CCATGG	1 cut(s)	858
NdeII	GATC	9 cut(s)	21, 146, 468, 543, 547, 578, 837, 1276, 1354
NlaIV	GGNNCC	3 cut(s)	600, 1386, 1531
NmuCI	GTSAC	2 cut(s)	219, 1132
NsbI	TGCGCA	1 cut(s)	761
NsiI	ATGCAT	2 cut(s)	1037, 1041
NspI	RCATGY	1 cut(s)	1039
PaeI	GCATGC	1 cut(s)	1039
PasI	CCCWGGG	1 cut(s)	737
PceI	AGGCCT	1 cut(s)	1221
PfeI	GAWTC	5 cut(s)	397, 765, 1024, 1258, 1447
PflMI	CCANNNNNTGG	1 cut(s)	1526
PinAI	ACCGGT	1 cut(s)	527
PkrI	GCNGC	2 cut(s)	656, 700
Ple19I	CGATCG	1 cut(s)	550
PmaCI	CACGTG	2 cut(s)	245, 710
PmlI	CACGTG	2 cut(s)	245, 710
Ppu21I	YACGTR	2 cut(s)	245, 710
PshBI	ATTAAT	2 cut(s)	359, 500
Psp124BI	GAGCTC	1 cut(s)	1473
Psp6I	CCWGG	1 cut(s)	736
PspCI	CACGTG	2 cut(s)	245, 710
PspFI	CCCAGC	2 cut(s)	1231, 1235
PspGI	CCWGG	1 cut(s)	736
PspN4I	GGNNCC	3 cut(s)	600, 1386, 1531
PspPI	GGNCC	3 cut(s)	207, 453, 1385
PstI	CTGCAG	1 cut(s)	1548
PsuI	RGATCY	1 cut(s)	146
PvuI	CGATCG	1 cut(s)	550
PvuII	CAGCTG	1 cut(s)	1235
RseI	CAYNNNNRTG	1 cut(s)	1378
SacI	GAGCTC	1 cut(s)	1473
SaqAI	TTAA	5 cut(s)	359, 500, 563, 827, 1515
SatI	GCNGC	2 cut(s)	655, 699
Sau3AI	GATC	9 cut(s)	21, 146, 468, 543, 547, 578, 837, 1276, 1354
Sau96I	GGNCC	3 cut(s)	207, 453, 1385
ScrFI	CCNGG	1 cut(s)	738
SduI	GDGCHC	3 cut(s)	188, 1473, 1534
SfaNI	GCATC	3 cut(s)	144, 1048, 1355
SfcI	CTRYAG	1 cut(s)	1544
SinI	GGWCC	2 cut(s)	207, 453
SmiMI	CAYNNNNRTG	1 cut(s)	1378
SmlI	CTYRAG	2 cut(s)	491, 1466
SmoI	CTYRAG	2 cut(s)	491, 1466
SphI	GCATGC	1 cut(s)	1039
Sse9I	AATT	7 cut(s)	54, 419, 497, 564, 605, 613, 942
SseBI	AGGCCT	1 cut(s)	1221
SsiI	CCGC	1 cut(s)	699
SspI	AATATT	2 cut(s)	825, 1286
SspMI	CTAG	3 cut(s)	1175, 1389, 1400
SstI	GAGCTC	1 cut(s)	1473
StuI	AGGCCT	1 cut(s)	1221
StyD4I	CCNGG	1 cut(s)	736
StyI	CCWWGG	3 cut(s)	317, 435, 858
TaaI	ACNGT	1 cut(s)	906
TaiI	ACGT	3 cut(s)	191, 247, 712
TaqI	TCGA	3 cut(s)	546, 550, 1505
TaqII	GACCGA	1 cut(s)	1163
TasI	AATT	7 cut(s)	54, 419, 497, 564, 605, 613, 942
TauI	GCSGC	1 cut(s)	701
TfiI	GAWTC	5 cut(s)	397, 765, 1024, 1258, 1447
Tru1I	TTAA	5 cut(s)	359, 500, 563, 827, 1515
Tru9I	TTAA	5 cut(s)	359, 500, 563, 827, 1515
TscAI	CASTG	1 cut(s)	399
TseFI	GTSAC	2 cut(s)	219, 1132
TseI	GCWGC	1 cut(s)	654
Tsp45I	GTSAC	2 cut(s)	219, 1132
TspDTI	ATGAA	7 cut(s)	71, 188, 251, 370, 507, 757, 825
TspGWI	ACGGA	2 cut(s)	1067, 1501
TspRI	CASTG	1 cut(s)	399
Van91I	CCANNNNNTGG	1 cut(s)	1526
VpaK11BI	GGWCC	2 cut(s)	207, 453
VspI	ATTAAT	2 cut(s)	359, 500
XapI	RAATTY	2 cut(s)	605, 613
XceI	RCATGY	1 cut(s)	1039
XspI	CTAG	3 cut(s)	1175, 1389, 1400
Zsp2I	ATGCAT	2 cut(s)	1037, 1041

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.