Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 962 |
| AciI | CCGC | 2 cut(s) | 822, 939 |
| AcoI | YGGCCR | 1 cut(s) | 206 |
| AcsI | RAATTY | 1 cut(s) | 506 |
| AcuI | CTGAAG | 1 cut(s) | 525 |
| AcyI | GRCGYC | 1 cut(s) | 170 |
| AfaI | GTAC | 2 cut(s) | 654, 672 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 717, 818, 962 |
| AgsI | TTSAA | 3 cut(s) | 290, 511, 751 |
| AjnI | CCWGG | 2 cut(s) | 346, 519 |
| AluBI | AGCT | 4 cut(s) | 84, 165, 910, 968 |
| AluI | AGCT | 4 cut(s) | 84, 165, 910, 968 |
| Alw21I | GWGCWC | 2 cut(s) | 493, 970 |
| Alw44I | GTGCAC | 1 cut(s) | 489 |
| AoxI | GGCC | 4 cut(s) | 35, 206, 447, 873 |
| ApaLI | GTGCAC | 1 cut(s) | 489 |
| ApoI | RAATTY | 1 cut(s) | 506 |
| AspS9I | GGNCC | 1 cut(s) | 824 |
| AsuC2I | CCSGG | 1 cut(s) | 829 |
| AsuHPI | GGTGA | 7 cut(s) | 104, 177, 385, 536, 653, 700, 743 |
| AvaII | GGWCC | 1 cut(s) | 824 |
| BaeGI | GKGCMC | 1 cut(s) | 493 |
| BalI | TGGCCA | 1 cut(s) | 208 |
| BanII | GRGCYC | 2 cut(s) | 185, 970 |
| BbsI | GAAGAC | 1 cut(s) | 539 |
| Bbv12I | GWGCWC | 2 cut(s) | 493, 970 |
| BccI | CCATC | 8 cut(s) | 136, 217, 220, 345, 521, 853, 865, 925 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 11, 45 |
| BciT130I | CCWGG | 2 cut(s) | 348, 521 |
| BclI | TGATCA | 1 cut(s) | 972 |
| BcnI | CCSGG | 1 cut(s) | 829 |
| BfaI | CTAG | 1 cut(s) | 65 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 455 |
| BmcAI | AGTACT | 1 cut(s) | 672 |
| Bme1390I | CCNGG | 3 cut(s) | 348, 521, 829 |
| Bme18I | GGWCC | 1 cut(s) | 824 |
| BmgT120I | GGNCC | 1 cut(s) | 824 |
| BmiI | GGNNCC | 1 cut(s) | 826 |
| BmrFI | CCNGG | 3 cut(s) | 348, 521, 829 |
| BmsI | GCATC | 2 cut(s) | 9, 727 |
| BpiI | GAAGAC | 1 cut(s) | 539 |
| BpmI | CTGGAG | 1 cut(s) | 695 |
| BpuEI | CTTGAG | 1 cut(s) | 404 |
| BpuMI | CCSGG | 1 cut(s) | 829 |
| BsaHI | GRCGYC | 1 cut(s) | 170 |
| BsaJI | CCNNGG | 3 cut(s) | 520, 780, 827 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 668, 698 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 717, 818, 962 |
| Bse1I | ACTGG | 2 cut(s) | 38, 678 |
| BseBI | CCWGG | 2 cut(s) | 348, 521 |
| BseDI | CCNNGG | 3 cut(s) | 520, 780, 827 |
| BseGI | GGATG | 4 cut(s) | 205, 356, 390, 516 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 717, 818, 962 |
| BseMII | CTCAG | 1 cut(s) | 747 |
| BseNI | ACTGG | 2 cut(s) | 38, 678 |
| BseSI | GKGCMC | 1 cut(s) | 493 |
| BshFI | GGCC | 4 cut(s) | 37, 208, 449, 875 |
| BsiHKAI | GWGCWC | 2 cut(s) | 493, 970 |
| BsiSI | CCGG | 1 cut(s) | 829 |
| BslFI | GGGAC | 1 cut(s) | 810 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 717, 818, 962 |
| BsmFI | GGGAC | 1 cut(s) | 810 |
| BsnI | GGCC | 4 cut(s) | 37, 208, 449, 875 |
| Bsp1286I | GDGCHC | 3 cut(s) | 185, 493, 970 |
| Bsp143I | GATC | 2 cut(s) | 595, 972 |
| BspACI | CCGC | 2 cut(s) | 822, 939 |
| BspANI | GGCC | 4 cut(s) | 37, 208, 449, 875 |
| BspCNI | CTCAG | 1 cut(s) | 748 |
| BspLI | GGNNCC | 1 cut(s) | 826 |
| BsrI | ACTGG | 2 cut(s) | 38, 678 |
| BssECI | CCNNGG | 3 cut(s) | 520, 780, 827 |
| BssMI | GATC | 2 cut(s) | 595, 972 |
| BssNI | GRCGYC | 1 cut(s) | 170 |
| BssT1I | CCWWGG | 1 cut(s) | 780 |
| Bst2UI | CCWGG | 2 cut(s) | 348, 521 |
| Bst4CI | ACNGT | 3 cut(s) | 712, 725, 960 |
| BstACI | GRCGYC | 1 cut(s) | 170 |
| BstC8I | GCNNGC | 2 cut(s) | 82, 306 |
| BstDEI | CTNAG | 2 cut(s) | 369, 756 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 816 |
| BstF5I | GGATG | 4 cut(s) | 205, 356, 390, 516 |
| BstKTI | GATC | 2 cut(s) | 598, 975 |
| BstMBI | GATC | 2 cut(s) | 595, 972 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 310, 455, 464, 872, 938 |
| BstNI | CCWGG | 2 cut(s) | 348, 521 |
| BstSCI | CCNGG | 3 cut(s) | 346, 519, 827 |
| BstSLI | GKGCMC | 1 cut(s) | 493 |
| BstV2I | GAAGAC | 1 cut(s) | 539 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 527 |
| BsuRI | GGCC | 4 cut(s) | 37, 208, 449, 875 |
| BtsCI | GGATG | 4 cut(s) | 205, 356, 390, 516 |
| BtsI | GCAGTG | 1 cut(s) | 697 |
| BtsIMutI | CAGTG | 1 cut(s) | 697 |
| Cac8I | GCNNGC | 2 cut(s) | 82, 306 |
| Cfr13I | GGNCC | 1 cut(s) | 824 |
| CseI | GACGC | 1 cut(s) | 159 |
| Csp6I | GTAC | 2 cut(s) | 653, 671 |
| CviAII | CATG | 4 cut(s) | 23, 137, 189, 929 |
| CviQI | GTAC | 2 cut(s) | 653, 671 |
| DdeI | CTNAG | 2 cut(s) | 369, 756 |
| DpnI | GATC | 2 cut(s) | 597, 974 |
| DpnII | GATC | 2 cut(s) | 595, 972 |
| EaeI | YGGCCR | 1 cut(s) | 206 |
| Ecl136II | GAGCTC | 1 cut(s) | 968 |
| Eco130I | CCWWGG | 1 cut(s) | 780 |
| Eco24I | GRGCYC | 2 cut(s) | 185, 970 |
| Eco47I | GGWCC | 1 cut(s) | 824 |
| Eco53kI | GAGCTC | 1 cut(s) | 968 |
| Eco57I | CTGAAG | 1 cut(s) | 525 |
| EcoICRI | GAGCTC | 1 cut(s) | 968 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 816 |
| EcoRII | CCWGG | 2 cut(s) | 346, 519 |
| EcoT14I | CCWWGG | 1 cut(s) | 780 |
| EcoT22I | ATGCAT | 2 cut(s) | 24, 328 |
| EcoT38I | GRGCYC | 2 cut(s) | 185, 970 |
| ErhI | CCWWGG | 1 cut(s) | 780 |
| FaeI | CATG | 4 cut(s) | 26, 140, 192, 932 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 297, 329, 629, 661, 860, 892 |
| FaqI | GGGAC | 1 cut(s) | 810 |
| FatI | CATG | 4 cut(s) | 22, 136, 188, 928 |
| FbaI | TGATCA | 1 cut(s) | 972 |
| FokI | GGATG | 4 cut(s) | 212, 363, 397, 503 |
| FriOI | GRGCYC | 2 cut(s) | 185, 970 |
| FspBI | CTAG | 1 cut(s) | 65 |
| GsuI | CTGGAG | 1 cut(s) | 695 |
| HaeIII | GGCC | 4 cut(s) | 37, 208, 449, 875 |
| HapII | CCGG | 1 cut(s) | 829 |
| HgaI | GACGC | 1 cut(s) | 159 |
| Hin1I | GRCGYC | 1 cut(s) | 170 |
| Hin1II | CATG | 4 cut(s) | 26, 140, 192, 932 |
| HincII | GTYRAC | 1 cut(s) | 124 |
| HindII | GTYRAC | 1 cut(s) | 124 |
| HinfI | GANTC | 3 cut(s) | 500, 677, 949 |
| HpaII | CCGG | 1 cut(s) | 829 |
| HphI | GGTGA | 7 cut(s) | 104, 177, 385, 536, 653, 700, 743 |
| Hpy166II | GTNNAC | 5 cut(s) | 124, 340, 376, 416, 491 |
| Hpy188I | TCNGA | 3 cut(s) | 585, 769, 972 |
| Hpy188III | TCNNGA | 4 cut(s) | 243, 269, 421, 755 |
| Hpy8I | GTNNAC | 5 cut(s) | 124, 340, 376, 416, 491 |
| HpyAV | CCTTC | 6 cut(s) | 83, 266, 270, 439, 764, 945 |
| HpyCH4III | ACNGT | 3 cut(s) | 712, 725, 960 |
| HpyCH4IV | ACGT | 1 cut(s) | 988 |
| HpyCH4V | TGCA | 7 cut(s) | 22, 80, 326, 491, 619, 740, 837 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 310, 455, 464, 872, 938 |
| HpyF3I | CTNAG | 2 cut(s) | 369, 756 |
| HpySE526I | ACGT | 1 cut(s) | 988 |
| Hsp92I | GRCGYC | 1 cut(s) | 170 |
| Hsp92II | CATG | 4 cut(s) | 26, 140, 192, 932 |
| Ksp22I | TGATCA | 1 cut(s) | 972 |
| Kzo9I | GATC | 2 cut(s) | 595, 972 |
| LmnI | GCTCC | 3 cut(s) | 630, 937, 965 |
| LweI | GCATC | 2 cut(s) | 9, 727 |
| MaeI | CTAG | 1 cut(s) | 65 |
| MaeII | ACGT | 1 cut(s) | 988 |
| MaeIII | GTNAC | 2 cut(s) | 67, 712 |
| MalI | GATC | 2 cut(s) | 597, 974 |
| MboI | GATC | 2 cut(s) | 595, 972 |
| MboII | GAAGA | 4 cut(s) | 400, 544, 799, 880 |
| MhlI | GDGCHC | 3 cut(s) | 185, 493, 970 |
| MlsI | TGGCCA | 1 cut(s) | 208 |
| MluCI | AATT | 4 cut(s) | 146, 393, 506, 586 |
| MluNI | TGGCCA | 1 cut(s) | 208 |
| MlyI | GAGTC | 2 cut(s) | 509, 686 |
| MnlI | CCTC | 9 cut(s) | 108, 172, 348, 375, 397, 478, 604, 753, 812 |
| Mox20I | TGGCCA | 1 cut(s) | 208 |
| Mph1103I | ATGCAT | 2 cut(s) | 24, 328 |
| MscI | TGGCCA | 1 cut(s) | 208 |
| MseI | TTAA | 4 cut(s) | 44, 149, 297, 991 |
| Msp20I | TGGCCA | 1 cut(s) | 208 |
| MspI | CCGG | 1 cut(s) | 829 |
| MspR9I | CCNGG | 3 cut(s) | 348, 521, 829 |
| MvaI | CCWGG | 2 cut(s) | 348, 521 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 310, 455, 464, 872, 938 |
| NciI | CCSGG | 1 cut(s) | 829 |
| NdeII | GATC | 2 cut(s) | 595, 972 |
| NlaIII | CATG | 4 cut(s) | 26, 140, 192, 932 |
| NlaIV | GGNNCC | 1 cut(s) | 826 |
| NmuCI | GTSAC | 1 cut(s) | 712 |
| NsiI | ATGCAT | 2 cut(s) | 24, 328 |
| PfeI | GAWTC | 1 cut(s) | 949 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 962 |
| PleI | GAGTC | 2 cut(s) | 508, 685 |
| PpsI | GAGTC | 2 cut(s) | 508, 685 |
| Psp124BI | GAGCTC | 1 cut(s) | 970 |
| Psp6I | CCWGG | 2 cut(s) | 346, 519 |
| PspGI | CCWGG | 2 cut(s) | 346, 519 |
| PspN4I | GGNNCC | 1 cut(s) | 826 |
| PspPI | GGNCC | 1 cut(s) | 824 |
| RsaI | GTAC | 2 cut(s) | 654, 672 |
| RsaNI | GTAC | 2 cut(s) | 653, 671 |
| SacI | GAGCTC | 1 cut(s) | 970 |
| SaqAI | TTAA | 4 cut(s) | 44, 149, 297, 991 |
| Sau3AI | GATC | 2 cut(s) | 595, 972 |
| Sau96I | GGNCC | 1 cut(s) | 824 |
| ScaI | AGTACT | 1 cut(s) | 672 |
| SchI | GAGTC | 2 cut(s) | 509, 686 |
| ScrFI | CCNGG | 3 cut(s) | 348, 521, 829 |
| SduI | GDGCHC | 3 cut(s) | 185, 493, 970 |
| SfaNI | GCATC | 2 cut(s) | 9, 727 |
| SinI | GGWCC | 1 cut(s) | 824 |
| SmlI | CTYRAG | 1 cut(s) | 419 |
| SmoI | CTYRAG | 1 cut(s) | 419 |
| Sse9I | AATT | 4 cut(s) | 146, 393, 506, 586 |
| SsiI | CCGC | 2 cut(s) | 822, 939 |
| SspI | AATATT | 1 cut(s) | 285 |
| SspMI | CTAG | 1 cut(s) | 65 |
| SstI | GAGCTC | 1 cut(s) | 970 |
| StyD4I | CCNGG | 3 cut(s) | 346, 519, 827 |
| StyI | CCWWGG | 1 cut(s) | 780 |
| TaaI | ACNGT | 3 cut(s) | 712, 725, 960 |
| TaiI | ACGT | 1 cut(s) | 991 |
| TaqI | TCGA | 3 cut(s) | 244, 598, 952 |
| TasI | AATT | 4 cut(s) | 146, 393, 506, 586 |
| TatI | WGTACW | 2 cut(s) | 652, 670 |
| TfiI | GAWTC | 1 cut(s) | 949 |
| Tru1I | TTAA | 4 cut(s) | 44, 149, 297, 991 |
| Tru9I | TTAA | 4 cut(s) | 44, 149, 297, 991 |
| TscAI | CASTG | 1 cut(s) | 704 |
| TseFI | GTSAC | 1 cut(s) | 712 |
| Tsp45I | GTSAC | 1 cut(s) | 712 |
| TspDTI | ATGAA | 2 cut(s) | 102, 401 |
| TspRI | CASTG | 1 cut(s) | 704 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 962 |
| VneI | GTGCAC | 1 cut(s) | 489 |
| VpaK11BI | GGWCC | 1 cut(s) | 824 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 816 |
| XapI | RAATTY | 1 cut(s) | 506 |
| XspI | CTAG | 1 cut(s) | 65 |
| ZrmI | AGTACT | 1 cut(s) | 672 |
| Zsp2I | ATGCAT | 2 cut(s) | 24, 328 |