Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 71, 569 |
| Acc16I | TGCGCA | 1 cut(s) | 5 |
| AccBSI | CCGCTC | 1 cut(s) | 57 |
| AccI | GTMKAC | 1 cut(s) | 177 |
| AccII | CGCG | 3 cut(s) | 123, 183, 640 |
| AciI | CCGC | 4 cut(s) | 38, 55, 126, 181 |
| AclI | AACGTT | 1 cut(s) | 428 |
| AclWI | GGATC | 2 cut(s) | 508, 749 |
| AcoI | YGGCCR | 2 cut(s) | 241, 674 |
| AcsI | RAATTY | 1 cut(s) | 246 |
| AcyI | GRCGYC | 1 cut(s) | 32 |
| AfaI | GTAC | 3 cut(s) | 113, 282, 444 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 69, 135, 592 |
| AgsI | TTSAA | 5 cut(s) | 316, 350, 434, 580, 700 |
| AjiI | CACGTC | 1 cut(s) | 51 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 260, 292 |
| AluBI | AGCT | 4 cut(s) | 381, 495, 651, 685 |
| AluI | AGCT | 4 cut(s) | 381, 495, 651, 685 |
| Alw21I | GWGCWC | 4 cut(s) | 50, 97, 383, 626 |
| Alw26I | GTCTC | 3 cut(s) | 360, 442, 507 |
| AlwI | GGATC | 2 cut(s) | 508, 749 |
| AoxI | GGCC | 3 cut(s) | 241, 320, 674 |
| ApeKI | GCWGC | 1 cut(s) | 685 |
| ApoI | RAATTY | 1 cut(s) | 246 |
| AspLEI | GCGC | 4 cut(s) | 6, 125, 185, 642 |
| AsuHPI | GGTGA | 1 cut(s) | 356 |
| AsuNHI | GCTAGC | 1 cut(s) | 491 |
| BaeGI | GKGCMC | 1 cut(s) | 425 |
| BalI | TGGCCA | 1 cut(s) | 676 |
| BanII | GRGCYC | 1 cut(s) | 383 |
| BbsI | GAAGAC | 2 cut(s) | 565, 604 |
| Bbv12I | GWGCWC | 4 cut(s) | 50, 97, 383, 626 |
| BbvI | GCAGC | 1 cut(s) | 672 |
| BccI | CCATC | 1 cut(s) | 369 |
| BceAI | ACGGC | 3 cut(s) | 25, 256, 276 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 515, 549 |
| BciVI | GTATCC | 1 cut(s) | 403 |
| BcoDI | GTCTC | 3 cut(s) | 360, 442, 507 |
| BfaI | CTAG | 4 cut(s) | 200, 372, 492, 689 |
| BfuI | GTATCC | 1 cut(s) | 403 |
| BisI | GCNGC | 3 cut(s) | 38, 126, 686 |
| BlpI | GCTNAGC | 1 cut(s) | 58 |
| BlsI | GCNGC | 3 cut(s) | 39, 127, 687 |
| BmgBI | CACGTC | 1 cut(s) | 51 |
| BmiI | GGNNCC | 1 cut(s) | 172 |
| BmtI | GCTAGC | 1 cut(s) | 495 |
| BpiI | GAAGAC | 2 cut(s) | 565, 604 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 58 |
| BsaHI | GRCGYC | 1 cut(s) | 32 |
| BsaJI | CCNNGG | 3 cut(s) | 166, 211, 323 |
| BsaWI | WCCGGW | 1 cut(s) | 251 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 69, 135, 592 |
| Bse1I | ACTGG | 1 cut(s) | 151 |
| BseDI | CCNNGG | 3 cut(s) | 166, 211, 323 |
| BseGI | GGATG | 1 cut(s) | 156 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 69, 135, 592 |
| BseMII | CTCAG | 3 cut(s) | 72, 122, 579 |
| BseNI | ACTGG | 1 cut(s) | 151 |
| BseRI | GAGGAG | 1 cut(s) | 87 |
| BseSI | GKGCMC | 1 cut(s) | 425 |
| BseXI | GCAGC | 1 cut(s) | 672 |
| BseYI | CCCAGC | 1 cut(s) | 129 |
| BsgI | GTGCAG | 1 cut(s) | 701 |
| Bsh1236I | CGCG | 3 cut(s) | 123, 183, 640 |
| Bsh1285I | CGRYCG | 1 cut(s) | 181 |
| BshFI | GGCC | 3 cut(s) | 243, 322, 676 |
| BsiEI | CGRYCG | 1 cut(s) | 181 |
| BsiHKAI | GWGCWC | 4 cut(s) | 50, 97, 383, 626 |
| BsiSI | CCGG | 2 cut(s) | 29, 252 |
| BslFI | GGGAC | 2 cut(s) | 37, 575 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 69, 135, 592 |
| BsmAI | GTCTC | 3 cut(s) | 360, 442, 507 |
| BsmFI | GGGAC | 2 cut(s) | 37, 575 |
| BsmI | GAATGC | 2 cut(s) | 340, 468 |
| BsnI | GGCC | 3 cut(s) | 243, 322, 676 |
| Bsp1286I | GDGCHC | 5 cut(s) | 50, 97, 383, 425, 626 |
| Bsp1407I | TGTACA | 1 cut(s) | 442 |
| Bsp143I | GATC | 3 cut(s) | 513, 587, 741 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 58 |
| BspACI | CCGC | 4 cut(s) | 38, 55, 126, 181 |
| BspANI | GGCC | 3 cut(s) | 243, 322, 676 |
| BspCNI | CTCAG | 3 cut(s) | 71, 121, 578 |
| BspFNI | CGCG | 3 cut(s) | 123, 183, 640 |
| BspHI | TCATGA | 1 cut(s) | 546 |
| BspLI | GGNNCC | 1 cut(s) | 172 |
| BspOI | GCTAGC | 1 cut(s) | 495 |
| BspPI | GGATC | 2 cut(s) | 508, 749 |
| BsrBI | CCGCTC | 1 cut(s) | 57 |
| BsrGI | TGTACA | 1 cut(s) | 442 |
| BsrI | ACTGG | 1 cut(s) | 151 |
| BssECI | CCNNGG | 3 cut(s) | 166, 211, 323 |
| BssMI | GATC | 3 cut(s) | 513, 587, 741 |
| BssNI | GRCGYC | 1 cut(s) | 32 |
| Bst4CI | ACNGT | 3 cut(s) | 68, 391, 449 |
| BstACI | GRCGYC | 1 cut(s) | 32 |
| BstAUI | TGTACA | 1 cut(s) | 442 |
| BstC8I | GCNNGC | 2 cut(s) | 493, 644 |
| BstDEI | CTNAG | 4 cut(s) | 58, 108, 565, 627 |
| BstF5I | GGATG | 1 cut(s) | 156 |
| BstFNI | CGCG | 3 cut(s) | 123, 183, 640 |
| BstHHI | GCGC | 4 cut(s) | 6, 125, 185, 642 |
| BstKTI | GATC | 3 cut(s) | 516, 590, 744 |
| BstMAI | GTCTC | 3 cut(s) | 360, 442, 507 |
| BstMBI | GATC | 3 cut(s) | 513, 587, 741 |
| BstMCI | CGRYCG | 1 cut(s) | 181 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 12, 29, 54, 648, 682 |
| BstSLI | GKGCMC | 1 cut(s) | 425 |
| BstUI | CGCG | 3 cut(s) | 123, 183, 640 |
| BstV1I | GCAGC | 1 cut(s) | 672 |
| BstV2I | GAAGAC | 2 cut(s) | 565, 604 |
| BstX2I | RGATCY | 2 cut(s) | 513, 741 |
| BstYI | RGATCY | 2 cut(s) | 513, 741 |
| BsuI | GTATCC | 1 cut(s) | 403 |
| BsuRI | GGCC | 3 cut(s) | 243, 322, 676 |
| BtrI | CACGTC | 1 cut(s) | 51 |
| BtsCI | GGATG | 1 cut(s) | 156 |
| BtsI | GCAGTG | 1 cut(s) | 460 |
| BtsIMutI | CAGTG | 2 cut(s) | 454, 460 |
| Cac8I | GCNNGC | 2 cut(s) | 493, 644 |
| CciI | TCATGA | 1 cut(s) | 546 |
| CfoI | GCGC | 4 cut(s) | 6, 125, 185, 642 |
| CseI | GACGC | 4 cut(s) | 21, 122, 238, 350 |
| Csp6I | GTAC | 3 cut(s) | 112, 281, 443 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 184, 219 |
| CviAII | CATG | 2 cut(s) | 547, 607 |
| CviQI | GTAC | 3 cut(s) | 112, 281, 443 |
| DdeI | CTNAG | 4 cut(s) | 58, 108, 565, 627 |
| DpnI | GATC | 3 cut(s) | 515, 589, 743 |
| DpnII | GATC | 3 cut(s) | 513, 587, 741 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 71, 569 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 71, 569 |
| EaeI | YGGCCR | 2 cut(s) | 241, 674 |
| Ecl136II | GAGCTC | 1 cut(s) | 381 |
| Eco147I | AGGCCT | 1 cut(s) | 322 |
| Eco24I | GRGCYC | 1 cut(s) | 383 |
| Eco53kI | GAGCTC | 1 cut(s) | 381 |
| EcoICRI | GAGCTC | 1 cut(s) | 381 |
| EcoT38I | GRGCYC | 1 cut(s) | 383 |
| FaeI | CATG | 2 cut(s) | 550, 610 |
| FaiI | YATR | 5 cut(s) | 483, 521, 531, 548, 608 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 501, 533 |
| FaqI | GGGAC | 2 cut(s) | 37, 575 |
| FatI | CATG | 2 cut(s) | 546, 606 |
| FauI | CCCGC | 1 cut(s) | 62 |
| FblI | GTMKAC | 1 cut(s) | 177 |
| Fnu4HI | GCNGC | 3 cut(s) | 38, 126, 686 |
| FokI | GGATG | 1 cut(s) | 143 |
| FriOI | GRGCYC | 1 cut(s) | 383 |
| Fsp4HI | GCNGC | 3 cut(s) | 38, 126, 686 |
| FspBI | CTAG | 4 cut(s) | 200, 372, 492, 689 |
| FspI | TGCGCA | 1 cut(s) | 5 |
| GlaI | GCGC | 4 cut(s) | 5, 124, 184, 641 |
| GluI | GCNGC | 3 cut(s) | 38, 126, 686 |
| GsaI | CCCAGC | 1 cut(s) | 133 |
| HaeIII | GGCC | 3 cut(s) | 243, 322, 676 |
| HapII | CCGG | 2 cut(s) | 29, 252 |
| HgaI | GACGC | 4 cut(s) | 21, 122, 238, 350 |
| HhaI | GCGC | 4 cut(s) | 6, 125, 185, 642 |
| Hin1I | GRCGYC | 1 cut(s) | 32 |
| Hin1II | CATG | 2 cut(s) | 550, 610 |
| Hin6I | GCGC | 4 cut(s) | 4, 123, 183, 640 |
| HinP1I | GCGC | 4 cut(s) | 4, 123, 183, 640 |
| HincII | GTYRAC | 2 cut(s) | 178, 309 |
| HindII | GTYRAC | 2 cut(s) | 178, 309 |
| HinfI | GANTC | 5 cut(s) | 191, 500, 563, 603, 693 |
| HpaII | CCGG | 2 cut(s) | 29, 252 |
| HphI | GGTGA | 1 cut(s) | 356 |
| Hpy166II | GTNNAC | 3 cut(s) | 178, 309, 445 |
| Hpy188I | TCNGA | 2 cut(s) | 385, 568 |
| Hpy188III | TCNNGA | 2 cut(s) | 547, 697 |
| Hpy8I | GTNNAC | 3 cut(s) | 178, 309, 445 |
| Hpy99I | CGWCG | 6 cut(s) | 37, 46, 68, 138, 179, 242 |
| HpyAV | CCTTC | 1 cut(s) | 262 |
| HpyCH4III | ACNGT | 3 cut(s) | 68, 391, 449 |
| HpyCH4IV | ACGT | 4 cut(s) | 50, 142, 305, 428 |
| HpyCH4V | TGCA | 2 cut(s) | 479, 718 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 12, 29, 54, 648, 682 |
| HpyF3I | CTNAG | 4 cut(s) | 58, 108, 565, 627 |
| HpySE526I | ACGT | 4 cut(s) | 50, 142, 305, 428 |
| Hsp92I | GRCGYC | 1 cut(s) | 32 |
| Hsp92II | CATG | 2 cut(s) | 550, 610 |
| HspAI | GCGC | 4 cut(s) | 4, 123, 183, 640 |
| Kzo9I | GATC | 3 cut(s) | 513, 587, 741 |
| LmnI | GCTCC | 3 cut(s) | 100, 176, 378 |
| Lsp1109I | GCAGC | 1 cut(s) | 672 |
| MaeI | CTAG | 4 cut(s) | 200, 372, 492, 689 |
| MaeII | ACGT | 4 cut(s) | 50, 142, 305, 428 |
| MaeIII | GTNAC | 2 cut(s) | 256, 362 |
| MalI | GATC | 3 cut(s) | 515, 589, 743 |
| MbiI | CCGCTC | 1 cut(s) | 57 |
| MboI | GATC | 3 cut(s) | 513, 587, 741 |
| MboII | GAAGA | 3 cut(s) | 565, 568, 604 |
| MfeI | CAATTG | 2 cut(s) | 406, 474 |
| MflI | RGATCY | 2 cut(s) | 513, 741 |
| MhlI | GDGCHC | 5 cut(s) | 50, 97, 383, 425, 626 |
| MlsI | TGGCCA | 1 cut(s) | 676 |
| MluCI | AATT | 4 cut(s) | 246, 406, 474, 486 |
| MluNI | TGGCCA | 1 cut(s) | 676 |
| MlyI | GAGTC | 3 cut(s) | 185, 509, 557 |
| Mox20I | TGGCCA | 1 cut(s) | 676 |
| MscI | TGGCCA | 1 cut(s) | 676 |
| MseI | TTAA | 2 cut(s) | 342, 557 |
| Msp20I | TGGCCA | 1 cut(s) | 676 |
| MspA1I | CMGCKG | 1 cut(s) | 685 |
| MspI | CCGG | 2 cut(s) | 29, 252 |
| MunI | CAATTG | 2 cut(s) | 406, 474 |
| Mva1269I | GAATGC | 2 cut(s) | 340, 468 |
| MvnI | CGCG | 3 cut(s) | 123, 183, 640 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 12, 29, 54, 648, 682 |
| NdeII | GATC | 3 cut(s) | 513, 587, 741 |
| NheI | GCTAGC | 1 cut(s) | 491 |
| NlaIII | CATG | 2 cut(s) | 550, 610 |
| NlaIV | GGNNCC | 1 cut(s) | 172 |
| NmeAIII | GCCGAG | 2 cut(s) | 147, 304 |
| NmuCI | GTSAC | 1 cut(s) | 362 |
| NsbI | TGCGCA | 1 cut(s) | 5 |
| PagI | TCATGA | 1 cut(s) | 546 |
| PceI | AGGCCT | 1 cut(s) | 322 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 41, 72, 174 |
| PctI | GAATGC | 2 cut(s) | 340, 468 |
| PfeI | GAWTC | 2 cut(s) | 603, 693 |
| PkrI | GCNGC | 3 cut(s) | 39, 127, 687 |
| PleI | GAGTC | 3 cut(s) | 185, 508, 557 |
| PpsI | GAGTC | 3 cut(s) | 185, 508, 557 |
| Psp124BI | GAGCTC | 1 cut(s) | 383 |
| Psp1406I | AACGTT | 1 cut(s) | 428 |
| PspFI | CCCAGC | 1 cut(s) | 129 |
| PspN4I | GGNNCC | 1 cut(s) | 172 |
| PsuI | RGATCY | 2 cut(s) | 513, 741 |
| PvuII | CAGCTG | 1 cut(s) | 685 |
| RsaI | GTAC | 3 cut(s) | 113, 282, 444 |
| RsaNI | GTAC | 3 cut(s) | 112, 281, 443 |
| SacI | GAGCTC | 1 cut(s) | 383 |
| SalI | GTCGAC | 1 cut(s) | 176 |
| SaqAI | TTAA | 2 cut(s) | 342, 557 |
| SatI | GCNGC | 3 cut(s) | 38, 126, 686 |
| Sau3AI | GATC | 3 cut(s) | 513, 587, 741 |
| SchI | GAGTC | 3 cut(s) | 185, 509, 557 |
| SduI | GDGCHC | 5 cut(s) | 50, 97, 383, 425, 626 |
| Sse9I | AATT | 4 cut(s) | 246, 406, 474, 486 |
| SseBI | AGGCCT | 1 cut(s) | 322 |
| SsiI | CCGC | 4 cut(s) | 38, 55, 126, 181 |
| SspMI | CTAG | 4 cut(s) | 200, 372, 492, 689 |
| SstI | GAGCTC | 1 cut(s) | 383 |
| StuI | AGGCCT | 1 cut(s) | 322 |
| TaaI | ACNGT | 3 cut(s) | 68, 391, 449 |
| TaiI | ACGT | 4 cut(s) | 53, 145, 308, 431 |
| TaqI | TCGA | 4 cut(s) | 44, 75, 177, 237 |
| TasI | AATT | 4 cut(s) | 246, 406, 474, 486 |
| TatI | WGTACW | 1 cut(s) | 442 |
| TauI | GCSGC | 2 cut(s) | 40, 128 |
| TfiI | GAWTC | 2 cut(s) | 603, 693 |
| Tru1I | TTAA | 2 cut(s) | 342, 557 |
| Tru9I | TTAA | 2 cut(s) | 342, 557 |
| TscAI | CASTG | 2 cut(s) | 454, 460 |
| TseFI | GTSAC | 1 cut(s) | 362 |
| TseI | GCWGC | 1 cut(s) | 685 |
| Tsp45I | GTSAC | 1 cut(s) | 362 |
| TspDTI | ATGAA | 1 cut(s) | 145 |
| TspGWI | ACGGA | 1 cut(s) | 163 |
| TspRI | CASTG | 2 cut(s) | 454, 460 |
| XapI | RAATTY | 1 cut(s) | 246 |
| XmiI | GTMKAC | 1 cut(s) | 177 |
| XspI | CTAG | 4 cut(s) | 200, 372, 492, 689 |