MD15G1050700.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000133
Physical Location & Seq
Reverse (-)
3459356 .. 3461853
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1050700.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGCATGGATGGACCTATAAATTCTTCACTACTTTGTCTCAGTTCCTTCAATTAGCTTTACTTTCACAGCAGCAATTAGCAAAACAAAAACAAAAGATGGAAGATTATCTTATTTTTATCAAAACGTTGGTATTTTCTTTGGGTTCTGTAATTCTCGTTTCTGTTCTGAGAGTTGTTCATGTCTACTGGTTTAGACCCAAGAGCTTGGAGAAGCAGTTGAGAAAGCAAGGGATCAGAGGCAAAACTTACAGGCTTTTCCAGGATGACATGAAAGAGATCAGCATGTCCAGCAAGGAAGCATGCGCCAAACCCATGTCTCTTAACCACCAGATTGCTCGACGTATCTTCCCTTTTTTTCATCAAATGGTGCAGAATTATGGAAAAGTGAGTTTGGGGTGGATTGAAACAAGGCCAAGGCTGATTGTAGCTGACCCAGAGCTGATCAAGTTGATATTAGCCGAAAAGAATGGGCAGATTACAAAGCCACCACTGAACCCACTTGTCAATCTTCTACAAATGGGCATCTCAACCTTGGAAGGAGAGCAATGGGCCAAACGCAGAAGGCTCATCAAACCTGCTTTCCACCTTGAGAAATTAAAGGGAATGGTGCCTGCATTTGTAACCTGTTGTTTTGGTCTGATCAATCGGTGGGAAAGTTTAGTTGGACATGAAGGAAGATGTGAACTAGATGTAGCTCCTGAATTTCAAAACCTTGCTGGTGATGTTATAGCTAGAACAGCCTTTGGAAGCAACTACGAAGAGGGGAAGAAAATATTTGAGCTTCAAAAAAAGCAAGCTATTCTAGTGCTTGAAGCCTACTATGATTTCTATTTTCCAGGCAAAAGATTCATACCCACTAAAAAGAACAGGATGAGGTACAACTTGGACAACGAAATCAAAGCAATATTAAGGGGTATGATCAGCAAGAAAGAGCAAGCAGCCATGGAAAATGGTGAAGAGGGTGCAAATGATTTGCTGGGCTTACTATTACAATGCAAAGAACAAGAGCAAACAAGCATGACGATTGAGGATGTCATAGAGGAGTGCAAGCTGTTCTACTTTGCTGGCCAAGAAACCACAGCCAACTGGCTGACTTGGGCTATGATTGTCTTATCTATGCATCCAAACTGGCAGGAAAAAGCAAGAGAAGAAGTCCTGCGTGTGTGTGGAAAGAACACACCTGATTTAGATGCTCTAAATCACCTTAAGATTGTTTCGATGATACTAAAGGAAGTTCTAAGGTTATATCCACCTGTGTCTGCTCTATACAGGCACACCCAAATCAAAACCAATGTTGGAGGCATTTCCATCCCAGCTGGGGTTGAATTTGTGCTGCTGACTATGTTTCTTCACTATGATAAAAAATGTTGGGGGGAAGACGTTGAGGAGTTCAACCCCGAGAGATTTGCTGATGGAGTTGTGAAGGCACCAAAGGATCAAGTTGTATTCTACCCATTTGGTTGGGGCCCTAGAATATGCATAGGGCAAACTTTTGCTGTGATAGAAGCAAAGATGGCTCTAGCTATGATTCTTCAGCATTTTTCATTTGAGCTCTCACCTTCTTACACTCATGCTCCTGTTATGGGTATTACCCTTCAGCCACAACATGGAGCCACAGTTACACTTCACAGAATTTAA

Protein Analysis

546

Amino Acids

62.61

Weight (kDa)

9.07

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 113 - 521 4.1e-81 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14610 AT3G14620 AT3G14630 AT3G14630 AT3G14640 AT3G14640 AT3G14650 AT3G14660 AT3G14660 AT3G14660 AT3G14680 AT3G14690 AT3G14690
fragaria_vesca FvH4_1g08410 FvH4_2g31040 FvH4_3g17200 FvH4_3g17200 FvH4_3g17220 FvH4_3g17220 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17240 FvH4_3g17260 FvH4_3g17260 FvH4_3g17263 FvH4_3g17264 FvH4_3g17265 FvH4_3g17266 FvH4_5g18530 FvH4_5g19690 FvH4_7g03990 FvH4_7g03990 FvH4_7g03990 FvH4_7g03990 FvH4_7g03990 FvH4_7g17900 FvH4_7g17900
malus_domestica MD02G1088200.v1.1 MD03G1224800.v1.1 MD03G1224900.v1.1 MD03G1225000.v1.1 MD11G1274100.v1.1 MD11G1274200.v1.1 MD11G1274300.v1.1 MD11G1274500.v1.1 MD11G1274600.v1.1 MD15G1050400.v1.1 MD15G1050500.v1.1 MD15G1050600.v1.1 MD15G1050700.v1.1
prunus_persica Prupe.1G404900_v2.0.a1 Prupe.1G404900_v2.0.a1 Prupe.4G152600_v2.0.a1 Prupe.4G152700_v2.0.a1 Prupe.4G152800_v2.0.a1 Prupe.4G152900_v2.0.a1 Prupe.4G153000_v2.0.a1 Prupe.7G201400_v2.0.a1 Prupe.7G201400_v2.0.a1
pyrus_communis pycom03g17310 pycom03g17330 pycom11g21520 pycom11g21600 pycom11g24180 pycom11g24190 pycom11g24200 pycom11g24210 pycom15g04890
rosa_chinensis RchiOBHm_Chr1g0327671 RchiOBHm_Chr1g0327711 RchiOBHm_Chr1g0327721 RchiOBHm_Chr2g0094501 RchiOBHm_Chr5g0028601 RchiOBHm_Chr5g0028611 RchiOBHm_Chr5g0028621 RchiOBHm_Chr5g0028661 RchiOBHm_Chr5g0028671 RchiOBHm_Chr5g0028701 RchiOBHm_Chr5g0028721 RchiOBHm_Chr5g0028741 RchiOBHm_Chr5g0028751 RchiOBHm_Chr5g0028771 RchiOBHm_Chr5g0028821 RchiOBHm_Chr6g0311761 RchiOBHm_Chr7g0204421
rosa_laevigata RLG00000003223 RLG00000003462 RLG00000010376 RLG00000012452 RLG00000016496 RLG00000018531 RLG00000018532 RLG00000018536 RLG00000029973 RLG00000029976 RLG00000033105 RLG00000033106 RLG00000033107 RLG00000033109 RLG00000033110 RLG00000033111 RLG00000033112 RLG00000033113 RLG00000033114 RLG00000033116 RLG00000033124
rosa_multiflora Rmu_co8029584.1_g000001 Rmu_co8159878.1_g000001 Rmu_co8310611.1_g000001 Rmu_sc0001000.1_g000004 Rmu_sc0001000.1_g000016 Rmu_sc0001575.1_g000020 Rmu_sc0001575.1_g000021 Rmu_sc0001575.1_g000022 Rmu_sc0001575.1_g000027 Rmu_sc0001575.1_g000035 Rmu_sc0001575.1_g000040 Rmu_sc0002888.1_g000039 Rmu_sc0003951.1_g000001 Rmu_sc0004359.1_g000045 Rmu_sc0005178.1_g000001 Rmu_sc0005178.1_g000002 Rmu_sc0005178.1_g000004 Rmu_sc0023855.1_g000001 Rmu_sc0024393.1_g000001 Rmu_sc0035326.1_g000001
rosa_roxburghii Rroxscaffold_1G00051080 Rroxscaffold_1G00051100 Rroxscaffold_1G00051110 Rroxscaffold_1G00051120 Rroxscaffold_1G00051150 Rroxscaffold_1G00051160 Rroxscaffold_1G00051170 Rroxscaffold_1G00051180 Rroxscaffold_2G00123610 Rroxscaffold_2G00147120 Rroxscaffold_3G00250540 Rroxscaffold_3G00253110 Rroxscaffold_3G00253120 Rroxscaffold_4G00322410 Rroxscaffold_4G00322440 Rroxscaffold_4G00322470 Rroxscaffold_7G00157330
rosa_rugosa Rorug01G0069500 Rorug01G0069600 Rorug01G0069700 Rorug02G0044200 Rorug02G0227700 Rorug05G0109800 Rorug05G0109900 Rorug05G0110000 Rorug05G0110100 Rorug05G0110300 Rorug05G0110400 Rorug05G0110500 Rorug07G0083800 Rorug07G0083900.1 Rorug07G0103700
rosa_samantha Rh1BG069000 Rh1BG069100 Rh1BG069200 Rh1CG084000 Rh1DG090700 Rh1DG090800 Rh1DG090900 Rh2AG092500 Rh2DG091600 Rh2DG309400 Rh5AG202100 Rh5AG202200 Rh5AG202500 Rh5AG202800 Rh5AG202900 Rh5CG220600 Rh5CG220700 Rh5CG220800 Rh5CG220900 Rh5CG221600 Rh5CG221900 Rh5CG222000 Rh5CG222100 Rh5DG203300 Rh5DG203400 Rh5DG203500 Rh5DG204100 Rh5DG204300 Rh5DG204400 Rh5DG204600 Rh6BG517600 Rh6CG523400 Rh6DG509100 Rh7AG213600 Rh7BG211400 Rh7DG220800
rosa_wichuraiana Rw0G010630 Rw1G006750 Rw1G006770 Rw5G018300 Rw5G018310 Rw5G018330 Rw6G044090 Rw7G018510 Rw7G020290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 583
AccB1I GGYRCC 2 cut(s) 607, 1426
AccB7I CCANNNNNTGG 1 cut(s) 1607
AccI GTMKAC 1 cut(s) 183
AclI AACGTT 1 cut(s) 125
AclWI GGATC 2 cut(s) 239, 1443
AcoI YGGCCR 1 cut(s) 1066
AcsI RAATTY 4 cut(s) 20, 701, 1325, 1632
AcuI CTGAAG 2 cut(s) 1517, 1580
AfaI GTAC 1 cut(s) 878
AfiI CCNNNNNNNGG 3 cut(s) 1318, 1583, 1607
AflII CTTAAG 1 cut(s) 1205
AgsI TTSAA 7 cut(s) 50, 404, 707, 785, 812, 1325, 1393
AjnI CCWGG 2 cut(s) 258, 835
AjuI GAANNNNNNNTTGG 2 cut(s) 645, 677
Alw21I GWGCWC 1 cut(s) 1554
Alw26I GTCTC 2 cut(s) 42, 321
AlwI GGATC 2 cut(s) 239, 1443
Ama87I CYCGRG 1 cut(s) 1397
AoxI GGCC 4 cut(s) 410, 549, 1066, 1465
ApaI GGGCCC 1 cut(s) 1469
ApeKI GCWGC 3 cut(s) 70, 938, 1333
ApoI RAATTY 4 cut(s) 20, 701, 1325, 1632
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 4 cut(s) 12, 549, 1465, 1466
AsuHPI GGTGA 4 cut(s) 731, 965, 1193, 1548
AvaI CYCGRG 1 cut(s) 1397
AvaII GGWCC 1 cut(s) 12
BaeGI GKGCMC 1 cut(s) 1469
BalI TGGCCA 1 cut(s) 1068
BanI GGYRCC 2 cut(s) 607, 1426
BanII GRGCYC 2 cut(s) 1469, 1554
BbsI GAAGAC 1 cut(s) 1383
Bbv12I GWGCWC 1 cut(s) 1554
BbvI GCAGC 3 cut(s) 82, 950, 1320
BccI CCATC 5 cut(s) 3, 91, 1316, 1406, 1507
BcgI CGANNNNNNTGC 2 cut(s) 1388, 1422
BciT130I CCWGG 2 cut(s) 260, 837
BclI TGATCA 3 cut(s) 441, 639, 918
BcoDI GTCTC 2 cut(s) 42, 321
BfaI CTAG 5 cut(s) 686, 732, 803, 1470, 1520
BfrI CTTAAG 1 cut(s) 1205
BfuAI ACCTGC 1 cut(s) 583
BisI GCNGC 3 cut(s) 71, 939, 1334
BlsI GCNGC 3 cut(s) 72, 940, 1335
Bme1390I CCNGG 2 cut(s) 260, 837
Bme18I GGWCC 1 cut(s) 12
BmeT110I CYCGRG 1 cut(s) 1397
BmgT120I GGNCC 4 cut(s) 12, 549, 1465, 1466
BmiI GGNNCC 5 cut(s) 609, 1428, 1466, 1467, 1612
BmrFI CCNGG 2 cut(s) 260, 837
BmsI GCATC 3 cut(s) 531, 1129, 1180
BpiI GAAGAC 1 cut(s) 1383
BpuEI CTTGAG 1 cut(s) 608
BsaJI CCNNGG 3 cut(s) 413, 531, 942
BsaXI ACNNNNNCTCC 6 cut(s) 200, 230, 1558, 1588, 1602, 1632
Bsc4I CCNNNNNNNGG 3 cut(s) 1318, 1583, 1607
Bse1I ACTGG 3 cut(s) 191, 1091, 1133
Bse3DI GCAATG 1 cut(s) 551
BseBI CCWGG 2 cut(s) 260, 837
BseDI CCNNGG 3 cut(s) 413, 531, 942
BseGI GGATG 6 cut(s) 14, 268, 876, 1036, 1120, 1308
BseLI CCNNNNNNNGG 3 cut(s) 1318, 1583, 1607
BseMI GCAATG 1 cut(s) 551
BseMII CTCAG 2 cut(s) 53, 158
BseNI ACTGG 3 cut(s) 191, 1091, 1133
BseRI GAGGAG 2 cut(s) 1055, 1400
BseSI GKGCMC 1 cut(s) 1469
BseXI GCAGC 3 cut(s) 82, 950, 1320
BseYI CCCAGC 3 cut(s) 976, 1312, 1316
BsgI GTGCAG 1 cut(s) 389
BshFI GGCC 4 cut(s) 412, 551, 1068, 1467
BshNI GGYRCC 2 cut(s) 607, 1426
BsiHKAI GWGCWC 1 cut(s) 1554
BsiHKCI CYCGRG 1 cut(s) 1397
BslI CCNNNNNNNGG 3 cut(s) 1318, 1583, 1607
BsmAI GTCTC 2 cut(s) 42, 321
BsnI GGCC 4 cut(s) 412, 551, 1068, 1467
BsoBI CYCGRG 1 cut(s) 1397
Bsp120I GGGCCC 1 cut(s) 1465
Bsp1286I GDGCHC 2 cut(s) 1469, 1554
Bsp143I GATC 6 cut(s) 231, 276, 441, 639, 918, 1435
Bsp19I CCATGG 1 cut(s) 942
BspANI GGCC 4 cut(s) 412, 551, 1068, 1467
BspCNI CTCAG 2 cut(s) 52, 159
BspLI GGNNCC 5 cut(s) 609, 1428, 1466, 1467, 1612
BspMI ACCTGC 1 cut(s) 583
BspPI GGATC 2 cut(s) 239, 1443
BspT107I GGYRCC 2 cut(s) 607, 1426
BspTI CTTAAG 1 cut(s) 1205
BsrDI GCAATG 1 cut(s) 551
BsrI ACTGG 3 cut(s) 191, 1091, 1133
BssECI CCNNGG 3 cut(s) 413, 531, 942
BssMI GATC 6 cut(s) 231, 276, 441, 639, 918, 1435
BssT1I CCWWGG 3 cut(s) 413, 531, 942
Bst2UI CCWGG 2 cut(s) 260, 837
Bst4CI ACNGT 1 cut(s) 1618
Bst6I CTCTTC 2 cut(s) 753, 951
BstAFI CTTAAG 1 cut(s) 1205
BstC8I GCNNGC 6 cut(s) 301, 612, 795, 936, 1049, 1066
BstDEI CTNAG 3 cut(s) 39, 167, 1238
BstDSI CCRYGG 1 cut(s) 942
BstF5I GGATG 6 cut(s) 14, 268, 876, 1036, 1120, 1308
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 6 cut(s) 234, 279, 444, 642, 921, 1438
BstMAI GTCTC 2 cut(s) 42, 321
BstMBI GATC 6 cut(s) 231, 276, 441, 639, 918, 1435
BstMWI GCNNNNNNNGC 3 cut(s) 288, 737, 1514
BstNI CCWGG 2 cut(s) 260, 837
BstNSI RCATGY 2 cut(s) 286, 303
BstSCI CCNGG 2 cut(s) 258, 835
BstSLI GKGCMC 1 cut(s) 1469
BstV1I GCAGC 3 cut(s) 82, 950, 1320
BstV2I GAAGAC 1 cut(s) 1383
BstXI CCANNNNNNTGG 2 cut(s) 205, 1461
BsuRI GGCC 4 cut(s) 412, 551, 1068, 1467
BtgI CCRYGG 1 cut(s) 942
BtsCI GGATG 6 cut(s) 14, 268, 876, 1036, 1120, 1308
BtsIMutI CAGTG 1 cut(s) 488
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 6 cut(s) 301, 612, 795, 936, 1049, 1066
CfoI GCGC 1 cut(s) 305
Cfr13I GGNCC 4 cut(s) 12, 549, 1465, 1466
Csp6I GTAC 1 cut(s) 877
CviQI GTAC 1 cut(s) 877
DdeI CTNAG 3 cut(s) 39, 167, 1238
DpnI GATC 6 cut(s) 233, 278, 443, 641, 920, 1437
DpnII GATC 6 cut(s) 231, 276, 441, 639, 918, 1435
EaeI YGGCCR 1 cut(s) 1066
Eam1104I CTCTTC 2 cut(s) 753, 951
EarI CTCTTC 2 cut(s) 753, 951
Ecl136II GAGCTC 1 cut(s) 1552
Eco130I CCWWGG 3 cut(s) 413, 531, 942
Eco24I GRGCYC 2 cut(s) 1469, 1554
Eco47I GGWCC 1 cut(s) 12
Eco53kI GAGCTC 1 cut(s) 1552
Eco57I CTGAAG 2 cut(s) 1517, 1580
Eco88I CYCGRG 1 cut(s) 1397
EcoICRI GAGCTC 1 cut(s) 1552
EcoO109I RGGNCCY 2 cut(s) 1465, 1466
EcoRII CCWGG 2 cut(s) 258, 835
EcoT14I CCWWGG 3 cut(s) 413, 531, 942
EcoT22I ATGCAT 3 cut(s) 6, 1122, 1481
EcoT38I GRGCYC 2 cut(s) 1469, 1554
ErhI CCWWGG 3 cut(s) 413, 531, 942
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FbaI TGATCA 3 cut(s) 441, 639, 918
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 3 cut(s) 71, 939, 1334
FokI GGATG 6 cut(s) 21, 275, 883, 1043, 1107, 1295
FriOI GRGCYC 2 cut(s) 1469, 1554
Fsp4HI GCNGC 3 cut(s) 71, 939, 1334
FspBI CTAG 5 cut(s) 686, 732, 803, 1470, 1520
GlaI GCGC 1 cut(s) 304
GluI GCNGC 3 cut(s) 71, 939, 1334
GsaI CCCAGC 3 cut(s) 980, 1316, 1320
HaeIII GGCC 4 cut(s) 412, 551, 1068, 1467
HhaI GCGC 1 cut(s) 305
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HinfI GANTC 2 cut(s) 846, 1528
HphI GGTGA 4 cut(s) 731, 965, 1193, 1548
Hpy166II GTNNAC 2 cut(s) 184, 683
Hpy188I TCNGA 3 cut(s) 168, 236, 639
Hpy188III TCNNGA 1 cut(s) 698
Hpy8I GTNNAC 2 cut(s) 184, 683
Hpy99I CGWCG 1 cut(s) 342
HpyAV CCTTC 7 cut(s) 56, 530, 555, 665, 1417, 1569, 1604
HpyCH4III ACNGT 1 cut(s) 1618
HpyCH4IV ACGT 3 cut(s) 125, 340, 1380
HpyCH4V TGCA 8 cut(s) 4, 370, 614, 965, 996, 1047, 1120, 1479
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 737, 1514
HpyF3I CTNAG 3 cut(s) 39, 167, 1238
HpySE526I ACGT 3 cut(s) 125, 340, 1380
HspAI GCGC 1 cut(s) 303
Ksp22I TGATCA 3 cut(s) 441, 639, 918
Kzo9I GATC 6 cut(s) 231, 276, 441, 639, 918, 1435
LmnI GCTCC 3 cut(s) 700, 1579, 1610
Lsp1109I GCAGC 3 cut(s) 82, 950, 1320
LweI GCATC 3 cut(s) 531, 1129, 1180
MaeI CTAG 5 cut(s) 686, 732, 803, 1470, 1520
MaeII ACGT 3 cut(s) 125, 340, 1380
MaeIII GTNAC 2 cut(s) 619, 1618
MalI GATC 6 cut(s) 233, 278, 443, 641, 920, 1437
MboI GATC 6 cut(s) 231, 276, 441, 639, 918, 1435
MhlI GDGCHC 2 cut(s) 1469, 1554
MlsI TGGCCA 1 cut(s) 1068
MluCI AATT 9 cut(s) 20, 50, 74, 150, 373, 593, 701, 1325, 1632
MluNI TGGCCA 1 cut(s) 1068
MmeI TCCRAC 2 cut(s) 643, 1276
MnlI CCTC 8 cut(s) 230, 754, 867, 952, 1021, 1033, 1292, 1378
Mox20I TGGCCA 1 cut(s) 1068
Mph1103I ATGCAT 3 cut(s) 6, 1122, 1481
MscI TGGCCA 1 cut(s) 1068
MseI TTAA 5 cut(s) 321, 596, 908, 1206, 1636
Msp20I TGGCCA 1 cut(s) 1068
MspA1I CMGCKG 1 cut(s) 1316
MspCI CTTAAG 1 cut(s) 1205
MspR9I CCNGG 2 cut(s) 260, 837
MvaI CCWGG 2 cut(s) 260, 837
MwoI GCNNNNNNNGC 3 cut(s) 288, 737, 1514
NcoI CCATGG 1 cut(s) 942
NdeII GATC 6 cut(s) 231, 276, 441, 639, 918, 1435
NlaIV GGNNCC 5 cut(s) 609, 1428, 1466, 1467, 1612
NsiI ATGCAT 3 cut(s) 6, 1122, 1481
NspI RCATGY 2 cut(s) 286, 303
PaeI GCATGC 1 cut(s) 303
PfeI GAWTC 2 cut(s) 846, 1528
PflMI CCANNNNNTGG 1 cut(s) 1607
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 3 cut(s) 72, 940, 1335
Psp124BI GAGCTC 1 cut(s) 1554
Psp1406I AACGTT 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 258, 835
PspFI CCCAGC 3 cut(s) 976, 1312, 1316
PspGI CCWGG 2 cut(s) 258, 835
PspN4I GGNNCC 5 cut(s) 609, 1428, 1466, 1467, 1612
PspOMI GGGCCC 1 cut(s) 1465
PspPI GGNCC 4 cut(s) 12, 549, 1465, 1466
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 1 cut(s) 878
RsaNI GTAC 1 cut(s) 877
SacI GAGCTC 1 cut(s) 1554
SaqAI TTAA 5 cut(s) 321, 596, 908, 1206, 1636
SatI GCNGC 3 cut(s) 71, 939, 1334
Sau3AI GATC 6 cut(s) 231, 276, 441, 639, 918, 1435
Sau96I GGNCC 4 cut(s) 12, 549, 1465, 1466
ScrFI CCNGG 2 cut(s) 260, 837
SduI GDGCHC 2 cut(s) 1469, 1554
SfaNI GCATC 3 cut(s) 531, 1129, 1180
SinI GGWCC 1 cut(s) 12
SmlI CTYRAG 2 cut(s) 587, 1205
SmoI CTYRAG 2 cut(s) 587, 1205
SphI GCATGC 1 cut(s) 303
Sse9I AATT 9 cut(s) 20, 50, 74, 150, 373, 593, 701, 1325, 1632
SspI AATATT 2 cut(s) 774, 906
SspMI CTAG 5 cut(s) 686, 732, 803, 1470, 1520
SstI GAGCTC 1 cut(s) 1554
StyD4I CCNGG 2 cut(s) 258, 835
StyI CCWWGG 3 cut(s) 413, 531, 942
TaaI ACNGT 1 cut(s) 1618
TaiI ACGT 3 cut(s) 128, 343, 1383
TaqI TCGA 2 cut(s) 337, 1217
TasI AATT 9 cut(s) 20, 50, 74, 150, 373, 593, 701, 1325, 1632
TfiI GAWTC 2 cut(s) 846, 1528
Tru1I TTAA 5 cut(s) 321, 596, 908, 1206, 1636
Tru9I TTAA 5 cut(s) 321, 596, 908, 1206, 1636
TscAI CASTG 1 cut(s) 495
TseI GCWGC 3 cut(s) 70, 938, 1333
TspDTI ATGAA 6 cut(s) 167, 284, 347, 684, 838, 1533
TspRI CASTG 1 cut(s) 495
Van91I CCANNNNNTGG 1 cut(s) 1607
Vha464I CTTAAG 1 cut(s) 1205
VpaK11BI GGWCC 1 cut(s) 12
XapI RAATTY 4 cut(s) 20, 701, 1325, 1632
XceI RCATGY 2 cut(s) 286, 303
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 5 cut(s) 686, 732, 803, 1470, 1520
Zsp2I ATGCAT 3 cut(s) 6, 1122, 1481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.