pycom08g00780

cinnamyl alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000739
Physical Location & Seq
Forward (+)
612555 .. 613296
742 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00780.2

Sequence Viewer

Length: 555 bp
ATGGTTCCCACAACCTCAAGTAGCTGCAACAGAAAGTCCGTGGAAGATGTCTGGACAGACATCAGCCTAGCGTCTCTTTCTCATAACTCCTGCAACACCACCGCCGCCGCCGTTCACGACCCTGCTGCCGCTGCTTTTCTCGGCATCATTTTTCAAGATTTCCTGGCTTCTACTAGTACTATTTCCTCCAACATCGACCCACCACCACCCGCTGCCGCCAGTACTGTTCTGAGCTTAAATTCCGGGACTCAATCTGATCTTGAACAGCGTTTACTTGAAACCACTAGGGCTACCATCACTGATCCTCGTCCATTGAAGCCAAACCCAAAACTCAACCCACTGGTACTAGTGGCTCTGGATGGTCATGATCAAAATCATGATGATCATCACAACTCCACCAACGGCGGCCGGCATAAGCGCATGATCAAGAATCGAGAGTCCGCTGCTCGCTCCAGAGCTTGTACAAATGAGTTGGAACTTGAAATTGCACATCTGCGGGAGGAGAATGCCAGGCTCAGGAGACAGCAAACAAGGATGATGATCCTTTTAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

185

Amino Acids

20.11

Weight (kDa)

6.7

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 9 cut(s) 102, 105, 108, 129, 210, 216, 405, 441, 496
AclWI GGATC 2 cut(s) 296, 535
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 1 cut(s) 238
AfaI GTAC 4 cut(s) 178, 223, 345, 463
AfiI CCNNNNNNNGG 1 cut(s) 516
AgsI TTSAA 5 cut(s) 155, 263, 278, 316, 482
AhlI ACTAGT 2 cut(s) 173, 346
AjnI CCWGG 2 cut(s) 162, 509
AluBI AGCT 3 cut(s) 24, 234, 458
AluI AGCT 3 cut(s) 24, 234, 458
Alw26I GTCTC 2 cut(s) 78, 514
AlwI GGATC 2 cut(s) 296, 535
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 5 cut(s) 24, 125, 131, 212, 443
ApoI RAATTY 1 cut(s) 238
AspLEI GCGC 1 cut(s) 420
AsuC2I CCSGG 1 cut(s) 244
BbvI GCAGC 5 cut(s) 11, 112, 118, 199, 430
BccI CCATC 2 cut(s) 302, 353
BceAI ACGGC 2 cut(s) 95, 418
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BciT130I CCWGG 2 cut(s) 164, 511
BclI TGATCA 3 cut(s) 367, 382, 423
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 2 cut(s) 78, 514
BcuI ACTAGT 2 cut(s) 173, 346
BfaI CTAG 4 cut(s) 68, 174, 285, 347
BmcAI AGTACT 2 cut(s) 178, 223
Bme1390I CCNGG 3 cut(s) 164, 244, 511
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 164, 244, 511
BmsI GCATC 1 cut(s) 153
BpmI CTGGAG 1 cut(s) 436
Bpu10I CCTNAGC 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 244
BsaBI GATNNNNATC 3 cut(s) 372, 384, 539
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 516
Bse118I RCCGGY 1 cut(s) 408
Bse1I ACTGG 2 cut(s) 219, 345
Bse8I GATNNNNATC 3 cut(s) 372, 384, 539
BseBI CCWGG 2 cut(s) 164, 511
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 2 cut(s) 364, 540
BseJI GATNNNNATC 3 cut(s) 372, 384, 539
BseLI CCNNNNNNNGG 1 cut(s) 516
BseMII CTCAG 2 cut(s) 221, 529
BseNI ACTGG 2 cut(s) 219, 345
BseRI GAGGAG 1 cut(s) 515
BseX3I CGGCCG 1 cut(s) 406
BseXI GCAGC 5 cut(s) 11, 112, 118, 199, 430
Bsh1285I CGRYCG 1 cut(s) 409
BshFI GGCC 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 409
BsiSI CCGG 2 cut(s) 243, 409
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 1 cut(s) 516
BsmAI GTCTC 2 cut(s) 78, 514
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 1 cut(s) 408
Bsp1407I TGTACA 1 cut(s) 461
Bsp143I GATC 6 cut(s) 256, 301, 367, 382, 423, 540
BspACI CCGC 9 cut(s) 102, 105, 108, 129, 210, 216, 405, 441, 496
BspANI GGCC 1 cut(s) 408
BspCNI CTCAG 2 cut(s) 222, 528
BspHI TCATGA 2 cut(s) 364, 376
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 296, 535
BsrFI RCCGGY 1 cut(s) 408
BsrGI TGTACA 1 cut(s) 461
BsrI ACTGG 2 cut(s) 219, 345
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 6 cut(s) 256, 301, 367, 382, 423, 540
Bst2UI CCWGG 2 cut(s) 164, 511
Bst4CI ACNGT 1 cut(s) 226
BstAUI TGTACA 1 cut(s) 461
BstC8I GCNNGC 2 cut(s) 410, 448
BstDEI CTNAG 2 cut(s) 230, 515
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 2 cut(s) 364, 540
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 6 cut(s) 259, 304, 370, 385, 426, 543
BstMAI GTCTC 2 cut(s) 78, 514
BstMBI GATC 6 cut(s) 256, 301, 367, 382, 423, 540
BstMCI CGRYCG 1 cut(s) 409
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 2 cut(s) 164, 511
BstSCI CCNGG 3 cut(s) 162, 242, 509
BstV1I GCAGC 5 cut(s) 11, 112, 118, 199, 430
BstZI CGGCCG 1 cut(s) 406
BsuRI GGCC 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 2 cut(s) 364, 540
BtsIMutI CAGTG 2 cut(s) 297, 338
Cac8I GCNNGC 2 cut(s) 410, 448
CciI TCATGA 2 cut(s) 364, 376
CfoI GCGC 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 408
CseI GACGC 1 cut(s) 60
Csp6I GTAC 4 cut(s) 177, 222, 344, 462
CviAII CATG 3 cut(s) 365, 377, 421
CviQI GTAC 4 cut(s) 177, 222, 344, 462
DdeI CTNAG 2 cut(s) 230, 515
DpnI GATC 6 cut(s) 258, 303, 369, 384, 425, 542
DpnII GATC 6 cut(s) 256, 301, 367, 382, 423, 540
EaeI YGGCCR 1 cut(s) 406
EagI CGGCCG 1 cut(s) 406
EclXI CGGCCG 1 cut(s) 406
Eco52I CGGCCG 1 cut(s) 406
EcoRII CCWGG 2 cut(s) 162, 509
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 3 cut(s) 368, 380, 424
FaiI YATR 5 cut(s) 84, 366, 378, 414, 422
FaqI GGGAC 1 cut(s) 259
FatI CATG 3 cut(s) 364, 376, 420
FauI CCCGC 2 cut(s) 217, 489
FbaI TGATCA 3 cut(s) 367, 382, 423
FokI GGATG 2 cut(s) 371, 547
FspBI CTAG 4 cut(s) 68, 174, 285, 347
GlaI GCGC 1 cut(s) 419
GsuI CTGGAG 1 cut(s) 436
HaeIII GGCC 1 cut(s) 408
HapII CCGG 2 cut(s) 243, 409
HgaI GACGC 1 cut(s) 60
HhaI GCGC 1 cut(s) 420
Hin1II CATG 3 cut(s) 368, 380, 424
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HinfI GANTC 3 cut(s) 247, 430, 437
HpaII CCGG 2 cut(s) 243, 409
Hpy166II GTNNAC 2 cut(s) 115, 272
Hpy188I TCNGA 2 cut(s) 231, 256
Hpy8I GTNNAC 2 cut(s) 115, 272
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4V TGCA 3 cut(s) 27, 93, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 2 cut(s) 230, 515
Hsp92II CATG 3 cut(s) 368, 380, 424
HspAI GCGC 1 cut(s) 418
KroI GCCGGC 1 cut(s) 408
KroNI GCCGGC 1 cut(s) 410
Ksp22I TGATCA 3 cut(s) 367, 382, 423
Kzo9I GATC 6 cut(s) 256, 301, 367, 382, 423, 540
LmnI GCTCC 1 cut(s) 455
Lsp1109I GCAGC 5 cut(s) 11, 112, 118, 199, 430
LweI GCATC 1 cut(s) 153
MaeI CTAG 4 cut(s) 68, 174, 285, 347
MalI GATC 6 cut(s) 258, 303, 369, 384, 425, 542
MboI GATC 6 cut(s) 256, 301, 367, 382, 423, 540
MboII GAAGA 1 cut(s) 56
MluCI AATT 2 cut(s) 238, 483
MlyI GAGTC 2 cut(s) 241, 446
MmeI TCCRAC 2 cut(s) 213, 453
MnlI CCTC 4 cut(s) 25, 196, 315, 493
MroNI GCCGGC 1 cut(s) 408
MseI TTAA 1 cut(s) 236
MspA1I CMGCKG 3 cut(s) 131, 212, 443
MspI CCGG 2 cut(s) 243, 409
MspR9I CCNGG 3 cut(s) 164, 244, 511
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 2 cut(s) 164, 511
MwoI GCNNNNNNNGC 1 cut(s) 131
NaeI GCCGGC 1 cut(s) 410
NciI CCSGG 1 cut(s) 244
NdeII GATC 6 cut(s) 256, 301, 367, 382, 423, 540
NgoMIV GCCGGC 1 cut(s) 408
NlaIII CATG 3 cut(s) 368, 380, 424
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 120
PagI TCATGA 2 cut(s) 364, 376
PctI GAATGC 1 cut(s) 511
PdiI GCCGGC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 430
PfoI TCCNGGA 1 cut(s) 242
PleI GAGTC 2 cut(s) 241, 445
PpsI GAGTC 2 cut(s) 241, 445
Psp6I CCWGG 2 cut(s) 162, 509
PspGI CCWGG 2 cut(s) 162, 509
PspN4I GGNNCC 1 cut(s) 6
PsrI GAACNNNNNNTAC 2 cut(s) 255, 287
RsaI GTAC 4 cut(s) 178, 223, 345, 463
RsaNI GTAC 4 cut(s) 177, 222, 344, 462
SaqAI TTAA 1 cut(s) 236
Sau3AI GATC 6 cut(s) 256, 301, 367, 382, 423, 540
ScaI AGTACT 2 cut(s) 178, 223
SchI GAGTC 2 cut(s) 241, 446
ScrFI CCNGG 3 cut(s) 164, 244, 511
SetI ASST 4 cut(s) 17, 26, 236, 460
SfaNI GCATC 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
SpeI ACTAGT 2 cut(s) 173, 346
Sse9I AATT 2 cut(s) 238, 483
SsiI CCGC 9 cut(s) 102, 105, 108, 129, 210, 216, 405, 441, 496
SspMI CTAG 4 cut(s) 68, 174, 285, 347
StyD4I CCNGG 3 cut(s) 162, 242, 509
TaaI ACNGT 1 cut(s) 226
TaqI TCGA 2 cut(s) 195, 433
TasI AATT 2 cut(s) 238, 483
TatI WGTACW 3 cut(s) 176, 221, 461
TauI GCSGC 5 cut(s) 107, 110, 131, 218, 408
TfiI GAWTC 1 cut(s) 430
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TscAI CASTG 2 cut(s) 304, 345
TseI GCWGC 5 cut(s) 24, 125, 131, 212, 443
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 2 cut(s) 304, 345
XapI RAATTY 1 cut(s) 238
XspI CTAG 4 cut(s) 68, 174, 285, 347
ZrmI AGTACT 2 cut(s) 178, 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.