Rh1AG062500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000739
Physical Location & Seq
Reverse (-)
10446123 .. 10465753
19631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG062500.1

Sequence Viewer

Length: 432 bp
ATGCGTCCTCCTTCGGTTGGCGACAGCGTTGCGCCTCGGATCAAGAAGGATGAGGTCATCCTCCTCTGCGATTCAAATCTTGATAGCGATGGCAGGGAAGGATCTTGGTCCCCAATTGATCTGATTGTTGAAGTCCTAGGGGCTGTTGAATGTTGGAGGTTGTGGAGGCCAGGGATTCAGGTGCGTTGGGTTGGTGCTGATGGCTTTCACATGGCACAATTGAAGGTGAAAGTCGAGCTAAAGGACTGGGCTTTGGGGTTCCTTGAACAGGGGGTCTGTGATAGAGGCGGTACAGTCAGCGTAGTGGGATGGTCTACGGCGGCGGCTGGTGAACAGGAAGGGATGCTAGGGTTTCCTTGTACTGGGCCCGAATTAAAATCTGACCTCTTTGCCATACACTATAAATATCCAGGTACTCAACATGAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

143

Amino Acids

15.67

Weight (kDa)

4.99

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 314
AciI CCGC 3 cut(s) 288, 320, 323
AclWI GGATC 2 cut(s) 47, 109
AfaI GTAC 3 cut(s) 292, 361, 415
AfiI CCNNNNNNNGG 3 cut(s) 17, 268, 362
AgsI TTSAA 5 cut(s) 75, 131, 149, 223, 266
AjnI CCWGG 2 cut(s) 169, 409
AluBI AGCT 1 cut(s) 238
AluI AGCT 1 cut(s) 238
AlwI GGATC 2 cut(s) 47, 109
AoxI GGCC 2 cut(s) 167, 365
ApaI GGGCCC 1 cut(s) 369
AspA2I CCTAGG 1 cut(s) 136
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 3 cut(s) 108, 365, 366
AsuHPI GGTGA 2 cut(s) 238, 341
AvaII GGWCC 1 cut(s) 108
AvrII CCTAGG 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 369
BanII GRGCYC 1 cut(s) 369
BccI CCATC 3 cut(s) 83, 194, 303
BceAI ACGGC 1 cut(s) 333
BcgI CGANNNNNNTGC 2 cut(s) 11, 45
BciT130I CCWGG 2 cut(s) 171, 411
BfaI CTAG 2 cut(s) 137, 347
BisI GCNGC 2 cut(s) 321, 324
BlnI CCTAGG 1 cut(s) 136
BlsI GCNGC 2 cut(s) 322, 325
Bme1390I CCNGG 2 cut(s) 171, 411
Bme18I GGWCC 1 cut(s) 108
BmgT120I GGNCC 3 cut(s) 108, 365, 366
BmiI GGNNCC 3 cut(s) 110, 260, 367
BmrFI CCNGG 2 cut(s) 171, 411
BmrI ACTGGG 2 cut(s) 256, 372
BmsI GCATC 1 cut(s) 333
BmuI ACTGGG 2 cut(s) 256, 372
BsaBI GATNNNNATC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 35, 136, 170
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 268, 362
Bse1I ACTGG 2 cut(s) 251, 367
Bse8I GATNNNNATC 1 cut(s) 75
BseBI CCWGG 2 cut(s) 171, 411
BseDI CCNNGG 3 cut(s) 35, 136, 170
BseGI GGATG 4 cut(s) 55, 57, 314, 348
BseJI GATNNNNATC 1 cut(s) 75
BseLI CCNNNNNNNGG 3 cut(s) 17, 268, 362
BseNI ACTGG 2 cut(s) 251, 367
BseRI GAGGAG 1 cut(s) 53
BseSI GKGCMC 1 cut(s) 369
BshFI GGCC 2 cut(s) 169, 367
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 3 cut(s) 17, 268, 362
BsmFI GGGAC 1 cut(s) 94
BsnI GGCC 2 cut(s) 169, 367
Bsp120I GGGCCC 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 3 cut(s) 39, 101, 118
BspACI CCGC 3 cut(s) 288, 320, 323
BspANI GGCC 2 cut(s) 169, 367
BspLI GGNNCC 3 cut(s) 110, 260, 367
BspPI GGATC 2 cut(s) 47, 109
BsrI ACTGG 2 cut(s) 251, 367
BssECI CCNNGG 3 cut(s) 35, 136, 170
BssMI GATC 3 cut(s) 39, 101, 118
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 2 cut(s) 171, 411
Bst4CI ACNGT 1 cut(s) 295
BstENI CCTNNNNNAGG 1 cut(s) 266
BstF5I GGATG 4 cut(s) 55, 57, 314, 348
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 3 cut(s) 42, 104, 121
BstMBI GATC 3 cut(s) 39, 101, 118
BstNI CCWGG 2 cut(s) 171, 411
BstSCI CCNGG 2 cut(s) 169, 409
BstSLI GKGCMC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BsuRI GGCC 2 cut(s) 169, 367
BtgZI GCGATG 1 cut(s) 102
BtsCI GGATG 4 cut(s) 55, 57, 314, 348
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 108, 365, 366
Csp6I GTAC 3 cut(s) 291, 360, 414
CviAII CATG 2 cut(s) 211, 422
CviJI RGCY 7 cut(s) 143, 169, 204, 238, 251, 326, 367
CviKI_1 RGCY 7 cut(s) 143, 169, 204, 238, 251, 326, 367
CviQI GTAC 3 cut(s) 291, 360, 414
DpnI GATC 3 cut(s) 41, 103, 120
DpnII GATC 3 cut(s) 39, 101, 118
Eco130I CCWWGG 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 108
EcoNI CCTNNNNNAGG 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 169, 409
EcoT14I CCWWGG 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 2 cut(s) 214, 425
FaiI YATR 4 cut(s) 212, 395, 402, 423
FaqI GGGAC 1 cut(s) 94
FatI CATG 2 cut(s) 210, 421
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 2 cut(s) 321, 324
FokI GGATG 4 cut(s) 44, 62, 321, 355
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 2 cut(s) 321, 324
FspBI CTAG 2 cut(s) 137, 347
GlaI GCGC 1 cut(s) 33
GluI GCNGC 2 cut(s) 321, 324
HaeIII GGCC 2 cut(s) 169, 367
HhaI GCGC 1 cut(s) 34
Hin1II CATG 2 cut(s) 214, 425
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HinfI GANTC 2 cut(s) 71, 175
HphI GGTGA 2 cut(s) 238, 341
Hpy166II GTNNAC 2 cut(s) 315, 332
Hpy188I TCNGA 3 cut(s) 39, 123, 382
Hpy188III TCNNGA 2 cut(s) 43, 80
Hpy8I GTNNAC 2 cut(s) 315, 332
HpyAV CCTTC 5 cut(s) 21, 40, 92, 217, 332
HpyCH4III ACNGT 1 cut(s) 295
Hsp92II CATG 2 cut(s) 214, 425
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 3 cut(s) 39, 101, 118
LweI GCATC 1 cut(s) 333
MaeI CTAG 2 cut(s) 137, 347
MalI GATC 3 cut(s) 41, 103, 120
MboI GATC 3 cut(s) 39, 101, 118
MfeI CAATTG 2 cut(s) 114, 218
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 3 cut(s) 114, 218, 371
MmeI TCCRAC 1 cut(s) 134
MnlI CCTC 9 cut(s) 18, 45, 46, 71, 74, 150, 159, 278, 395
MseI TTAA 2 cut(s) 374, 430
MspR9I CCNGG 2 cut(s) 171, 411
MunI CAATTG 2 cut(s) 114, 218
MvaI CCWGG 2 cut(s) 171, 411
NdeII GATC 3 cut(s) 39, 101, 118
NlaIII CATG 2 cut(s) 214, 425
NlaIV GGNNCC 3 cut(s) 110, 260, 367
PfeI GAWTC 2 cut(s) 71, 175
PkrI GCNGC 2 cut(s) 322, 325
Psp6I CCWGG 2 cut(s) 169, 409
PspGI CCWGG 2 cut(s) 169, 409
PspN4I GGNNCC 3 cut(s) 110, 260, 367
PspOMI GGGCCC 1 cut(s) 365
PspPI GGNCC 3 cut(s) 108, 365, 366
PsuI RGATCY 1 cut(s) 101
RsaI GTAC 3 cut(s) 292, 361, 415
RsaNI GTAC 3 cut(s) 291, 360, 414
SaqAI TTAA 2 cut(s) 374, 430
SatI GCNGC 2 cut(s) 321, 324
Sau3AI GATC 3 cut(s) 39, 101, 118
Sau96I GGNCC 3 cut(s) 108, 365, 366
ScrFI CCNGG 2 cut(s) 171, 411
SduI GDGCHC 1 cut(s) 369
SetI ASST 7 cut(s) 57, 161, 183, 228, 240, 387, 415
SfaNI GCATC 1 cut(s) 333
SinI GGWCC 1 cut(s) 108
Sse9I AATT 3 cut(s) 114, 218, 371
SsiI CCGC 3 cut(s) 288, 320, 323
SspMI CTAG 2 cut(s) 137, 347
StyD4I CCNGG 2 cut(s) 169, 409
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 295
TaqI TCGA 1 cut(s) 234
TasI AATT 3 cut(s) 114, 218, 371
TatI WGTACW 1 cut(s) 359
TauI GCSGC 2 cut(s) 323, 326
TfiI GAWTC 2 cut(s) 71, 175
Tru1I TTAA 2 cut(s) 374, 430
Tru9I TTAA 2 cut(s) 374, 430
VpaK11BI GGWCC 1 cut(s) 108
XagI CCTNNNNNAGG 1 cut(s) 266
XmaJI CCTAGG 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 314
XspI CTAG 2 cut(s) 137, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.