RLG00000035356

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000739
Physical Location & Seq
Reverse (-)
65777781 .. 65780187
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035356

Sequence Viewer

Length: 513 bp
ATGGCGGCTGTGAAGGCGGTGCGGTATGGTCTCAAGTCTGGCAGCGAGGTGGATTTATCTGGGTGGCTCGTTGACGGTGGAAGGGATGAGAGCGAGCCTTTTGGCTGTTTCATGTTTCTACCCATGTCGTTCGACCCGGGTGGATTGGATCTGGTATTGGCCGTGTTCGGCAGTGCTATCCTGGTCGAGGAGGATAGCGGCGATGTGGTGGCTGGTTTGGACGATTGGTTGGGTGTGATGGGATCGGTGTTGGTCGTAGTAGCGCCGGCGGTCTTGTTGCTGTCGGTGGAGATTCTGTGTAGGCTAGGTGCTGCCGGACTGGTAATGAGTCATATGGCTGGGCCCTTGGGTTGGCTCTGCTCGAAGGCTGGACGTGGTTTCGGCTTGGTTCTGTTTCGTTGCTCTGAGGCGGTGATGGATGGGAGCACTGAGGGCGAGGTTGGTTCCAAGCGGCGGTGGCGATGCAGCGACGTTTGCTCAGTTGGCAGCGTCAAGGTGCAGCCCTCGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

17.75

Weight (kDa)

4.62

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 5, 17, 22, 198, 269, 410, 451, 454
AclWI GGATC 2 cut(s) 156, 250
AcoI YGGCCR 1 cut(s) 159
AfiI CCNNNNNNNGG 3 cut(s) 187, 351, 453
AjiI CACGTC 1 cut(s) 374
AjnI CCWGG 1 cut(s) 180
Alw21I GWGCWC 1 cut(s) 428
Alw26I GTCTC 1 cut(s) 35
AlwI GGATC 2 cut(s) 156, 250
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 2 cut(s) 159, 341
ApaI GGGCCC 1 cut(s) 345
ApeKI GCWGC 5 cut(s) 42, 311, 465, 486, 499
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 2 cut(s) 341, 342
AsuC2I CCSGG 2 cut(s) 137, 138
AsuHPI GGTGA 1 cut(s) 424
AvaI CYCGRG 1 cut(s) 136
BaeGI GKGCMC 1 cut(s) 345
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 428
BbvI GCAGC 4 cut(s) 54, 298, 477, 498
BccI CCATC 3 cut(s) 232, 409, 413
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 182
BcnI CCSGG 2 cut(s) 137, 138
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 1 cut(s) 305
BfoI RGCGCY 1 cut(s) 266
BisI GCNGC 8 cut(s) 6, 43, 199, 312, 452, 466, 487, 500
BlsI GCNGC 8 cut(s) 7, 44, 200, 313, 453, 467, 488, 501
Bme1390I CCNGG 3 cut(s) 137, 138, 182
BmeT110I CYCGRG 1 cut(s) 136
BmgBI CACGTC 1 cut(s) 374
BmgT120I GGNCC 2 cut(s) 341, 342
BmiI GGNNCC 2 cut(s) 343, 445
BmrFI CCNGG 3 cut(s) 137, 138, 182
BmsI GCATC 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 17
BpuMI CCSGG 2 cut(s) 137, 138
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 136, 345
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 351, 453
Bse118I RCCGGY 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 324
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 2 cut(s) 136, 345
BseGI GGATG 2 cut(s) 91, 424
BseLI CCNNNNNNNGG 3 cut(s) 187, 351, 453
BseMII CTCAG 3 cut(s) 396, 420, 492
BseNI ACTGG 1 cut(s) 324
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 4 cut(s) 54, 298, 477, 498
BseYI CCCAGC 1 cut(s) 338
BshFI GGCC 2 cut(s) 161, 343
BsiHKAI GWGCWC 1 cut(s) 428
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 3 cut(s) 137, 266, 315
BslI CCNNNNNNNGG 3 cut(s) 187, 351, 453
BsmAI GTCTC 1 cut(s) 35
BsnI GGCC 2 cut(s) 161, 343
Bso31I GGTCTC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 136
Bsp120I GGGCCC 1 cut(s) 341
Bsp1286I GDGCHC 2 cut(s) 345, 428
Bsp143I GATC 2 cut(s) 148, 242
BspACI CCGC 8 cut(s) 5, 17, 22, 198, 269, 410, 451, 454
BspANI GGCC 2 cut(s) 161, 343
BspCNI CTCAG 3 cut(s) 397, 421, 491
BspLI GGNNCC 2 cut(s) 343, 445
BspPI GGATC 2 cut(s) 156, 250
BspTNI GGTCTC 1 cut(s) 35
BsrFI RCCGGY 1 cut(s) 265
BsrI ACTGG 1 cut(s) 324
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 2 cut(s) 136, 345
BssMI GATC 2 cut(s) 148, 242
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 95, 267
BstDEI CTNAG 3 cut(s) 405, 429, 478
BstENI CCTNNNNNAGG 1 cut(s) 185
BstF5I GGATG 2 cut(s) 91, 424
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 2 cut(s) 151, 245
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 2 cut(s) 148, 242
BstMWI GCNNNNNNNGC 5 cut(s) 14, 432, 457, 474, 483
BstNI CCWGG 1 cut(s) 182
BstSCI CCNGG 3 cut(s) 135, 136, 180
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 4 cut(s) 54, 298, 477, 498
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
BsuRI GGCC 2 cut(s) 161, 343
BtgZI GCGATG 2 cut(s) 216, 475
BtrI CACGTC 1 cut(s) 374
BtsCI GGATG 2 cut(s) 91, 424
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 2 cut(s) 178, 426
Cac8I GCNNGC 2 cut(s) 95, 267
CfoI GCGC 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 2 cut(s) 341, 342
Cfr9I CCCGGG 1 cut(s) 136
CseI GACGC 1 cut(s) 478
CviAII CATG 2 cut(s) 112, 124
DdeI CTNAG 3 cut(s) 405, 429, 478
DpnI GATC 2 cut(s) 150, 244
DpnII GATC 2 cut(s) 148, 242
EaeI YGGCCR 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 342
EcoRII CCWGG 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 2 cut(s) 115, 127
FaiI YATR 5 cut(s) 27, 113, 125, 333, 335
FatI CATG 2 cut(s) 111, 123
FauNDI CATATG 1 cut(s) 333
Fnu4HI GCNGC 8 cut(s) 6, 43, 199, 312, 452, 466, 487, 500
FokI GGATG 2 cut(s) 98, 431
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 8 cut(s) 6, 43, 199, 312, 452, 466, 487, 500
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 264
GluI GCNGC 8 cut(s) 6, 43, 199, 312, 452, 466, 487, 500
GsaI CCCAGC 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 266
HaeIII GGCC 2 cut(s) 161, 343
HapII CCGG 3 cut(s) 137, 266, 315
HgaI GACGC 1 cut(s) 478
HhaI GCGC 1 cut(s) 265
Hin1II CATG 2 cut(s) 115, 127
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 2 cut(s) 292, 328
HpaII CCGG 3 cut(s) 137, 266, 315
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 406, 512
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 3 cut(s) 7, 75, 358
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 2 cut(s) 373, 471
HpyCH4V TGCA 2 cut(s) 465, 499
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 432, 457, 474, 483
HpyF3I CTNAG 3 cut(s) 405, 429, 478
HpySE526I ACGT 2 cut(s) 373, 471
Hsp92II CATG 2 cut(s) 115, 127
HspAI GCGC 1 cut(s) 263
KroI GCCGGC 1 cut(s) 265
KroNI GCCGGC 1 cut(s) 267
Kzo9I GATC 2 cut(s) 148, 242
LmnI GCTCC 1 cut(s) 423
Lsp1109I GCAGC 4 cut(s) 54, 298, 477, 498
LweI GCATC 1 cut(s) 452
MaeI CTAG 1 cut(s) 305
MaeII ACGT 2 cut(s) 373, 471
MalI GATC 2 cut(s) 150, 244
MboI GATC 2 cut(s) 148, 242
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 2 cut(s) 345, 428
MlyI GAGTC 1 cut(s) 337
MnlI CCTC 6 cut(s) 40, 181, 184, 400, 424, 430
MreI CGCCGGCG 1 cut(s) 265
MroNI GCCGGC 1 cut(s) 265
MspI CCGG 3 cut(s) 137, 266, 315
MspR9I CCNGG 3 cut(s) 137, 138, 182
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 5 cut(s) 14, 432, 457, 474, 483
NaeI GCCGGC 1 cut(s) 267
NciI CCSGG 2 cut(s) 137, 138
NdeI CATATG 1 cut(s) 333
NdeII GATC 2 cut(s) 148, 242
NgoMIV GCCGGC 1 cut(s) 265
NlaIII CATG 2 cut(s) 115, 127
NlaIV GGNNCC 2 cut(s) 343, 445
PdiI GCCGGC 1 cut(s) 267
PfeI GAWTC 1 cut(s) 292
PkrI GCNGC 8 cut(s) 7, 44, 200, 313, 453, 467, 488, 501
PleI GAGTC 1 cut(s) 336
PpsI GAGTC 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 338
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 2 cut(s) 343, 445
PspOMI GGGCCC 1 cut(s) 341
PspPI GGNCC 2 cut(s) 341, 342
PsuI RGATCY 1 cut(s) 148
SatI GCNGC 8 cut(s) 6, 43, 199, 312, 452, 466, 487, 500
Sau3AI GATC 2 cut(s) 148, 242
Sau96I GGNCC 2 cut(s) 341, 342
SchI GAGTC 1 cut(s) 337
ScrFI CCNGG 3 cut(s) 137, 138, 182
SduI GDGCHC 2 cut(s) 345, 428
SetI ASST 6 cut(s) 51, 310, 376, 441, 474, 498
SfaNI GCATC 1 cut(s) 452
SgrAI CRCCGGYG 1 cut(s) 265
SmaI CCCGGG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SsiI CCGC 8 cut(s) 5, 17, 22, 198, 269, 410, 451, 454
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 3 cut(s) 135, 136, 180
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 2 cut(s) 376, 474
TaqI TCGA 3 cut(s) 132, 186, 362
TauI GCSGC 3 cut(s) 8, 201, 454
TfiI GAWTC 1 cut(s) 292
TscAI CASTG 2 cut(s) 178, 433
TseI GCWGC 5 cut(s) 42, 311, 465, 486, 499
TspDTI ATGAA 1 cut(s) 100
TspMI CCCGGG 1 cut(s) 136
TspRI CASTG 2 cut(s) 178, 433
XagI CCTNNNNNAGG 1 cut(s) 185
XmaI CCCGGG 1 cut(s) 136
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.