Rh3DG072100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000739
Physical Location & Seq
Reverse (-)
4887775 .. 4888206
432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG072100.1

Sequence Viewer

Length: 432 bp
ATGCATGGATTTTGTGGACTTGAGTCTGTCAGCAATGGAGATCCGGTGACGAGTTGTGGTTCACTGGTTATGATTCTGGAATCCGGATTGATTCAATCCCATTCCAATGGTTTCAATGTGGGTGGAAGTGATGCATTGTTTGATGCATGGCCGACGGCGCCGGAGCGGGAGCCTTCGTTTCAGATTCCTGACAGTGTCCATGACGGCGTTGATCTAAAGGATCGGGCTTTGGGGTTCCTTGAGCGGGGGTCTGTGATGGAGGTGGCACGGTCGACGTTAGTGGGGTGGTCTATGAAGGCGGCTAGTGAATGGGAAAGGATGCTAGGGTTTCCCTGTGTTGGGCTGTATTGGGTCTGTCTCTACTGGACTTGGACTGCCTATCTGATGTGGGCTTGGAAGATTTGGGTCTTTTTGGCCCATATTGGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

143

Amino Acids

15.88

Weight (kDa)

4.85

Isoelectric Point (pI)

25.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AccBSI CCGCTC 2 cut(s) 166, 244
AccI GTMKAC 1 cut(s) 272
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 3 cut(s) 166, 244, 299
AclWI GGATC 2 cut(s) 35, 228
AcoI YGGCCR 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 158
AfiI CCNNNNNNNGG 3 cut(s) 244, 338, 339
AgsI TTSAA 2 cut(s) 95, 115
Alw26I GTCTC 1 cut(s) 362
AlwI GGATC 2 cut(s) 35, 228
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 2 cut(s) 149, 414
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 58
BanI GGYRCC 1 cut(s) 157
BccI CCATC 1 cut(s) 250
BceAI ACGGC 2 cut(s) 171, 220
BcoDI GTCTC 1 cut(s) 362
BfaI CTAG 2 cut(s) 303, 323
BfoI RGCGCY 1 cut(s) 161
BisI GCNGC 1 cut(s) 300
BlsI GCNGC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 3 cut(s) 159, 171, 236
BmsI GCATC 3 cut(s) 121, 133, 309
BoxI GACNNNNGTC 1 cut(s) 22
BpuEI CTTGAG 2 cut(s) 41, 260
BsaHI GRCGYC 1 cut(s) 158
BsaWI WCCGGW 2 cut(s) 43, 83
BsaXI ACNNNNNCTCC 4 cut(s) 30, 60, 161, 191
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 338, 339
Bse1I ACTGG 2 cut(s) 69, 368
Bse3DI GCAATG 1 cut(s) 40
BseAI TCCGGA 1 cut(s) 83
BseGI GGATG 1 cut(s) 324
BseLI CCNNNNNNNGG 3 cut(s) 244, 338, 339
BseMI GCAATG 1 cut(s) 40
BseNI ACTGG 2 cut(s) 69, 368
Bsh1285I CGRYCG 1 cut(s) 272
BshFI GGCC 2 cut(s) 151, 416
BshNI GGYRCC 1 cut(s) 157
BsiEI CGRYCG 1 cut(s) 272
BsiSI CCGG 3 cut(s) 44, 84, 161
BslI CCNNNNNNNGG 3 cut(s) 244, 338, 339
BsmAI GTCTC 1 cut(s) 362
BsnI GGCC 2 cut(s) 151, 416
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 3 cut(s) 40, 211, 220
BspACI CCGC 3 cut(s) 166, 244, 299
BspANI GGCC 2 cut(s) 151, 416
BspEI TCCGGA 1 cut(s) 83
BspLI GGNNCC 3 cut(s) 159, 171, 236
BspPI GGATC 2 cut(s) 35, 228
BspT107I GGYRCC 1 cut(s) 157
BsrBI CCGCTC 2 cut(s) 166, 244
BsrDI GCAATG 1 cut(s) 40
BsrI ACTGG 2 cut(s) 69, 368
BssMI GATC 3 cut(s) 40, 211, 220
BssNI GRCGYC 1 cut(s) 158
Bst4CI ACNGT 2 cut(s) 194, 270
BstACI GRCGYC 1 cut(s) 158
BstF5I GGATG 1 cut(s) 324
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 43, 214, 223
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 3 cut(s) 40, 211, 220
BstMCI CGRYCG 1 cut(s) 272
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstPAI GACNNNNGTC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 40
BstXI CCANNNNNNTGG 1 cut(s) 107
BstYI RGATCY 1 cut(s) 40
BsuRI GGCC 2 cut(s) 151, 416
BtsCI GGATG 1 cut(s) 324
BtsIMutI CAGTG 2 cut(s) 62, 199
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 415
CspCI CAANNNNNGTGG 2 cut(s) 103, 138
CviAII CATG 3 cut(s) 5, 147, 200
CviJI RGCY 7 cut(s) 151, 172, 227, 302, 343, 392, 416
CviKI_1 RGCY 7 cut(s) 151, 172, 227, 302, 343, 392, 416
DinI GGCGCC 1 cut(s) 159
DpnI GATC 3 cut(s) 42, 213, 222
DpnII GATC 3 cut(s) 40, 211, 220
EaeI YGGCCR 1 cut(s) 149
EcoT22I ATGCAT 3 cut(s) 6, 136, 148
EgeI GGCGCC 1 cut(s) 159
EheI GGCGCC 1 cut(s) 159
FaeI CATG 3 cut(s) 8, 150, 203
FaiI YATR 6 cut(s) 6, 71, 148, 201, 293, 420
FatI CATG 3 cut(s) 4, 146, 199
FauI CCCGC 2 cut(s) 159, 237
FblI GTMKAC 1 cut(s) 272
Fnu4HI GCNGC 1 cut(s) 300
FokI GGATG 1 cut(s) 331
Fsp4HI GCNGC 1 cut(s) 300
FspBI CTAG 2 cut(s) 303, 323
GlaI GCGC 1 cut(s) 159
GluI GCNGC 1 cut(s) 300
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 2 cut(s) 151, 416
HapII CCGG 3 cut(s) 44, 84, 161
HhaI GCGC 1 cut(s) 160
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 3 cut(s) 8, 150, 203
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 5 cut(s) 23, 73, 80, 91, 184
HpaII CCGG 3 cut(s) 44, 84, 161
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 3 cut(s) 17, 62, 273
Hpy188I TCNGA 2 cut(s) 183, 384
Hpy188III TCNNGA 3 cut(s) 77, 84, 188
Hpy8I GTNNAC 3 cut(s) 17, 62, 273
Hpy99I CGWCG 2 cut(s) 157, 277
HpyAV CCTTC 2 cut(s) 183, 289
HpyCH4III ACNGT 2 cut(s) 194, 270
HpyCH4IV ACGT 1 cut(s) 275
HpyCH4V TGCA 3 cut(s) 4, 134, 146
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpySE526I ACGT 1 cut(s) 275
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 3 cut(s) 8, 150, 203
HspAI GCGC 1 cut(s) 158
KasI GGCGCC 1 cut(s) 157
Kpn2I TCCGGA 1 cut(s) 83
Kzo9I GATC 3 cut(s) 40, 211, 220
LmnI GCTCC 2 cut(s) 163, 169
LpnPI CCDG 8 cut(s) 50, 57, 62, 97, 174, 201, 346, 349
LweI GCATC 3 cut(s) 121, 133, 309
MaeI CTAG 2 cut(s) 303, 323
MaeII ACGT 1 cut(s) 275
MaeIII GTNAC 1 cut(s) 46
MalI GATC 3 cut(s) 42, 213, 222
MbiI CCGCTC 2 cut(s) 166, 244
MboI GATC 3 cut(s) 40, 211, 220
MboII GAAGA 1 cut(s) 409
MflI RGATCY 1 cut(s) 40
Mly113I GGCGCC 1 cut(s) 158
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 1 cut(s) 253
Mph1103I ATGCAT 3 cut(s) 6, 136, 148
MroI TCCGGA 1 cut(s) 83
MseI TTAA 1 cut(s) 430
MslI CAYNNNNRTG 2 cut(s) 105, 423
MspI CCGG 3 cut(s) 44, 84, 161
MwoI GCNNNNNNNGC 1 cut(s) 157
NarI GGCGCC 1 cut(s) 158
NdeII GATC 3 cut(s) 40, 211, 220
NlaIII CATG 3 cut(s) 8, 150, 203
NlaIV GGNNCC 3 cut(s) 159, 171, 236
NmuCI GTSAC 1 cut(s) 46
NsiI ATGCAT 3 cut(s) 6, 136, 148
PfeI GAWTC 4 cut(s) 73, 80, 91, 184
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 1 cut(s) 301
PleI GAGTC 1 cut(s) 31
PluTI GGCGCC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 31
PshAI GACNNNNGTC 1 cut(s) 22
PspN4I GGNNCC 3 cut(s) 159, 171, 236
PspPI GGNCC 1 cut(s) 415
PsuI RGATCY 1 cut(s) 40
PsyI GACNNNGTC 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 105, 423
SalI GTCGAC 1 cut(s) 271
SaqAI TTAA 1 cut(s) 430
SatI GCNGC 1 cut(s) 300
Sau3AI GATC 3 cut(s) 40, 211, 220
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 1 cut(s) 32
SetI ASST 2 cut(s) 264, 278
SfaNI GCATC 3 cut(s) 121, 133, 309
SfoI GGCGCC 1 cut(s) 159
SmiMI CAYNNNNRTG 2 cut(s) 105, 423
SmlI CTYRAG 2 cut(s) 20, 239
SmoI CTYRAG 2 cut(s) 20, 239
SsiI CCGC 3 cut(s) 166, 244, 299
SspDI GGCGCC 1 cut(s) 157
SspMI CTAG 2 cut(s) 303, 323
TaaI ACNGT 2 cut(s) 194, 270
TaiI ACGT 1 cut(s) 278
TaqI TCGA 1 cut(s) 272
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 4 cut(s) 73, 80, 91, 184
Tru1I TTAA 1 cut(s) 430
Tru9I TTAA 1 cut(s) 430
TscAI CASTG 2 cut(s) 69, 199
TseFI GTSAC 1 cut(s) 46
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 308
TspRI CASTG 2 cut(s) 69, 199
Tth111I GACNNNGTC 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 272
XspI CTAG 2 cut(s) 303, 323
Zsp2I ATGCAT 3 cut(s) 6, 136, 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.