pycom17g27240

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000654
Physical Location & Seq
Forward (+)
25518008 .. 25524029
6022 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3531 bp
ATGGATGCTTCCCGAGATTCTTCTTCCTCCTCTGACCGGTATTCCTATCATGCCTTCTTGAGTTTCAGAGGCCAGGACACTCGCAAGGGCTTTACTGATCACCTCTATAGAGCCATGGAGCAAGCAGGAATCCACACGTTTAGAGACGATGATGAAATCGAGAGAGGGGAGAATATTGAGTCGGAGTTAGACAAGGCGATACAGGAGTCACAAGTCTCAATAATTGTCTTCTCCAAGGACTACGCTTCCTCAAGGTGGTGTTTGAACGAACTTCTGAAGATCGTGAAACGTAAAAAAGATGATCGTAGACATGTGGTTTTGCCAGTTTTCTATGATGTGGATCCATCCGATGTCAGGAAGCAGAGTGGTTCTTTTGCTGAAGCATTTGCCGGACATAAGGAGCGATTCAGTACGGAGATGGACAAGGTGGAGCAGTGGAGAAGAGCTCTTGGAGATGTTGCATCTTTGGCAGGCATGGTTTTAGGAGATCGGTATGAGGGGCAGTTCATCCAAGATATTGTTGAACAGATTAGAAATAAACTGGATCACGCAGCTTTAGACGTTGCTCCCTTTTCAGTTGGAATGGATTATCGTGTGCAACGCCTAAACATGTGGTTACAAGATCGATCAAATGATGTTGGTATAGCTGTCATCTGTGGGATGGGTGGAATTGGCAAGAGCACTATCGCGAAAGCCGTTTATAACCGCAATTTTGATAGATTTCAAGGTAGCAGCTTTCTTGTAGATATACGAGAATCTTCAGAACAACCCAGTGGTTTTGTTGGCTTGCAAAGAAAACTTCTTTCAGATATCCAAAAGGGGAAAGCAAAGAAAGTATACAATATGGACGAAGGAACAAGCAGGATCAAACAAGCCGTGGGCTATAAAAAAGTTCTTATTGTTCTTGATGATGTGAGTGATCGGGATCAACTCAAAGCAATTCTTGGAATGCGAGAGTGGCTTCATCCGGGAAGTAAAATTATTGTAACAACTCGACATGAACATTTGTTAAATGCTCATGAAGTTTTTGAAAAGTTTGTGGTTCGAGAACTGGATGAGGATGAATCACTTGAGCTTTTCAGTTGGCATGCCTTCAGACAATCCCATCCAGAAGAAGGTTACATGGAGCTTTCAAGACATGTGGTACAGCACTGTGGAGGGCTTCCATTAGCTCTTGAAGTTCTGGGATCTTCTTTATCCGGAAAAAGTGTAGAAGTATGGCAAAGTGCATTGCAGAAGTTAGACGTCATCCCTAATGACAAAATTCAAAAAGTTCTGAGGATAAGTTTTGATTCTTTACCAGATGATCATGACAAAAATTTATTCCTTCACATAGCCAGTTTCTTCACAGGAAAGAAGAAGGATTATACGAGTACAATGTTGGACAGCTTAAAGTTTTGCACAAGGATTGGGATTGAAAATCTAGTTGACAGATGCCTAGTGAAAATTCAAAGTAACAGGTTGGTCATGCATCAATTGGTGAGAGACATGGCAATGGAAGTTATTCGTAAAGAATCACCTGATGACCCTGGAAAACGTAGTAAAGTGTTGCAGAAAGATGCCTCTAATATTTTGGGAAAATTAAGTGGTACGGAAAATATTAAGGGCCTCATGCTCAACCTTGCTAGCAAAAGAACGTTTGTTGGATGTCATAAAAAACGATGCCACGATGAAGATTATGATGGAAATTGTTCAAGACGACGTCGGCTTGGTATCTTCGCTTGGAAGGCAATTAGCTTTTCATCCACAAACTCAGCTTCTGCATCAAATGAGACAGATTTCGAAGCTGAGGCGTTTAGAAGGATGCACAATCTTGAACTTCTCATGCTCAACAACGTCAACGTCAGGGGAAGCTACGAAGAATTTCCAAAAAGTTTAATATGTTTATCTTGGAGAGGATTCCACTTAAAATCAGTACCAGAAAATTTTTATTTGGAAAACCTGGTTGTTCTTGACATGCGAAAAAGTAGCCTACAACATGTTTGGAATGGGATCAGGTCTCTTCCGAGGCTGAAAATTCTTGATCTCAGTCATTCGCATGGCCTTAGGACAACCTCCAACTTCTCAGGACTCCCTAACCTTGAGAGATTGATTCTTAAACATTGCATAAATTTGGTTGAGGTTGATGAGTCCATCGGATACCTAGAGAAACTTGTTGTTTTGAATCTGGAAGGCTGCAAAAATCTCATGAAGCTTCCAAAATTGAGATCTAGTGAGGATCTTATTCTTTCTGGTTGCTCAAAGCTTGAGCTTGGTGCCAATACGACTGCCACAACTGATCACTTACAATCTACATCATGGTATTCAATCTGGTCGTGGGTGTCGCTGAGAAAAAATATTGAATCAAGCAGTTTCTCATTGGCAAGTTTACCGCATTCCTTAACAAGTTTACGTCTGGATCGCTGCAATCTATCAGAGATTCCGAGTGCCCTTACTATGCTGCCTTCGTTGGAGTACTTGAATCTGACTTACAATCCAATTACAAGCCTACCAGAGAGCATGAACAATCTTGTTAAGCTTCTCACTCTTGACTTGGAAAGTTGCAGAAACCTCACAATGCTTCCAGAGCTCCCACATAGTTTGAAACAATTGAATGTTATAGGTTGCACATCATTGAAAAGAATAACAAATTTACCGAACTTGCACATATCACCGGCCACGCCCCCACCTCCCTTCTATGGCGACATAAGGGGCTCACATTTTACAAGGGTTGATAACGTATTAACGAACTCAGATTTATGGTATTGTGGGAAACTAGTTGATGTTAGCAGCTTGTTCCGTACACTAACATTGAGCAGCTTTGACATAAAAGTCCTCAAAGATATCGATCGATCCGATTTGAAACCCAACGGTGGCATTGAGGTCCAAAGGAACAACTATTGTGCAAGTAGGCGCTCCAAGGGAACTTATGAATGCGCCATATATAGCATTTTTCTCCATGAGAGCAAGATTCCAGATCGGTTCAATTACAGGAGCATGGGTAACACTGTTTTCTCTATTATTGTTCCTTCACAGCCTAATATCAAGATCGTAGGTGTAAATGCATGTGTATTGTATGCCCGGCAATCGGATCGAAGCCGTGAGCATTTCCCTTCGATTCAACTTAGGAATGAGACAAAGGGCCTTAGGTGGGGATTTTTGTGGGCGGAAAAGGTTGAATGGAAAGTGATGAACGAAGATATGTTATGGCTAACCCATTGGATTATGGGGAACAATGAATTGGAATGCGGGGAAGAAGTGTGTTTTAAAATCAAAGAGCAAGACGATTTACTCGCAAAGGAGATTGGAGTCCAGTTTGTGTACGAGCAGCAAAATAAGGATAAGGAGGATGTCCCATCAAGTAGTGAAGATACAACGATCCAACGCGAAAGATTGCCTCGTAACATGCAAGGGAACTGCATATTCGGTCCGAGATGGTTCCATAACTGCGAGAATGAATCTATGTCAACTGTCGTCTGCAATGTTTATGAATATCTCAAAAACCATGAAGTGATCGATCTTGAGAATGACTCCCGCATGACCCCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1177

Amino Acids

134.46

Weight (kDa)

6.95

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 184 4.9e-51 TIR domain
TIR_2 PF13676 19 - 115 9.4e-17 TIR domain
NB-ARC PF00931 197 - 368 2.4e-21 NB-ARC domain
WHD_ROQ1 PF23282 436 - 506 6.4e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_8 PF13855 793 - 849 5.3e-08 Leucine rich repeat
LRR_14 PF23598 794 - 874 1e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AasI GACNNNNNNGTC 1 cut(s) 2810
AatII GACGTC 2 cut(s) 1248, 1705
AccB1I GGYRCC 1 cut(s) 2255
AccI GTMKAC 2 cut(s) 307, 837
AccII CGCG 2 cut(s) 689, 3366
AccIII TCCGGA 1 cut(s) 1199
AciI CCGC 5 cut(s) 706, 2372, 3146, 3228, 3514
AclI AACGTT 1 cut(s) 1637
AcoI YGGCCR 1 cut(s) 2655
AcsI RAATTY 8 cut(s) 1263, 1318, 1446, 1862, 1924, 2016, 2110, 2629
AcuI CTGAAG 4 cut(s) 296, 399, 744, 1078
AcyI GRCGYC 2 cut(s) 1245, 1702
AfaI GTAC 8 cut(s) 412, 1146, 1375, 1591, 1917, 2456, 2782, 3302
AfiI CCNNNNNNNGG 8 cut(s) 36, 255, 354, 1115, 2678, 2852, 3067, 3130
AflIII ACRYGT 5 cut(s) 135, 310, 609, 1138, 1978
AgeI ACCGGT 1 cut(s) 36
AhlI ACTAGT 1 cut(s) 2755
AjnI CCWGG 3 cut(s) 72, 1528, 1941
AjuI GAANNNNNNNTTGG 2 cut(s) 3203, 3235
Alw21I GWGCWC 3 cut(s) 448, 683, 2571
Alw26I GTCTC 6 cut(s) 138, 220, 1479, 1766, 2004, 3107
AlwNI CAGNNNCTG 1 cut(s) 1760
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 1199
AoxI GGCC 5 cut(s) 70, 1606, 2041, 2655, 3121
ApeKI GCWGC 8 cut(s) 551, 732, 2175, 2403, 2440, 2769, 2796, 3307
ApoI RAATTY 8 cut(s) 1263, 1318, 1446, 1862, 1924, 2016, 2110, 2629
AsiGI ACCGGT 1 cut(s) 36
Asp700I GAANNNNTTC 3 cut(s) 1503, 1690, 1863
AspLEI GCGC 2 cut(s) 2895, 2918
AspS9I GGNCC 4 cut(s) 1606, 2863, 3121, 3407
AsuC2I CCSGG 2 cut(s) 969, 3061
AsuHPI GGTGA 4 cut(s) 92, 1492, 1509, 2643
AsuII TTCGAA 1 cut(s) 1782
AsuNHI GCTAGC 1 cut(s) 1625
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 2 cut(s) 2863, 3407
AxyI CCTNAGG 2 cut(s) 2045, 3125
BaeGI GKGCMC 1 cut(s) 2431
BaeI ACNNNNGTAYC 2 cut(s) 191, 224
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 1 cut(s) 2255
BanII GRGCYC 3 cut(s) 448, 2571, 2696
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 3 cut(s) 448, 683, 2571
BbvCI CCTCAGC 1 cut(s) 1788
BbvI GCAGC 8 cut(s) 563, 744, 2162, 2390, 2427, 2781, 2808, 3319
BccI CCATC 8 cut(s) 352, 412, 655, 1113, 1676, 2141, 3343, 3408
BceAI ACGGC 3 cut(s) 680, 860, 3063
BcgI CGANNNNNNTGC 2 cut(s) 3053, 3087
BciT130I CCWGG 3 cut(s) 74, 1530, 1943
BciVI GTATCC 1 cut(s) 2132
BclI TGATCA 3 cut(s) 97, 1306, 2278
BcnI CCSGG 2 cut(s) 969, 3061
BcoDI GTCTC 6 cut(s) 138, 220, 1479, 1766, 2004, 3107
BcuI ACTAGT 1 cut(s) 2755
BfaI CTAG 6 cut(s) 1424, 1439, 1626, 2144, 2211, 2756
BfmI CTRYAG 1 cut(s) 106
BfoI RGCGCY 1 cut(s) 2896
BfuI GTATCC 1 cut(s) 2132
BglII AGATCT 1 cut(s) 2207
BisI GCNGC 8 cut(s) 552, 733, 2176, 2404, 2441, 2770, 2797, 3308
BlsI GCNGC 8 cut(s) 553, 734, 2177, 2405, 2442, 2771, 2798, 3309
BmcAI AGTACT 1 cut(s) 2456
Bme1390I CCNGG 5 cut(s) 74, 969, 1530, 1943, 3061
Bme18I GGWCC 2 cut(s) 2863, 3407
BmeT110I CYCGRG 1 cut(s) 12
BmgT120I GGNCC 4 cut(s) 1606, 2863, 3121, 3407
BmiI GGNNCC 3 cut(s) 342, 2257, 3419
BmrFI CCNGG 5 cut(s) 74, 969, 1530, 1943, 3061
BmrI ACTGGG 1 cut(s) 765
BmsI GCATC 7 cut(s) 470, 1424, 1480, 1549, 1652, 1772, 1794
BmtI GCTAGC 1 cut(s) 1629
BmuI ACTGGG 1 cut(s) 765
BpiI GAAGAC 1 cut(s) 220
BplI GAGNNNNNCTC 4 cut(s) 430, 462, 3494, 3526
Bpu10I CCTNAGC 1 cut(s) 1788
Bpu14I TTCGAA 1 cut(s) 1782
BpuEI CTTGAG 6 cut(s) 79, 235, 1091, 2102, 2267, 3521
BpuMI CCSGG 2 cut(s) 969, 3061
Bsa29I ATCGAT 4 cut(s) 625, 2826, 2830, 3495
BsaBI GATNNNNATC 3 cut(s) 339, 924, 2826
BsaHI GRCGYC 2 cut(s) 1245, 1702
BsaI GGTCTC 1 cut(s) 2004
BsaJI CCNNGG 7 cut(s) 114, 234, 876, 1528, 2006, 2898, 3524
BsaWI WCCGGW 2 cut(s) 36, 1199
BsaXI ACNNNNNCTCC 2 cut(s) 2965, 2995
Bsc4I CCNNNNNNNGG 8 cut(s) 36, 255, 354, 1115, 2678, 2852, 3067, 3130
Bse118I RCCGGY 2 cut(s) 36, 2653
Bse1I ACTGG 6 cut(s) 323, 546, 771, 1056, 1338, 3292
Bse21I CCTNAGG 2 cut(s) 2045, 3125
Bse3DI GCAATG 4 cut(s) 1229, 1500, 2101, 3466
Bse8I GATNNNNATC 3 cut(s) 339, 924, 2826
BseAI TCCGGA 1 cut(s) 1199
BseBI CCWGG 3 cut(s) 74, 1530, 1943
BseCI ATCGAT 4 cut(s) 625, 2826, 2830, 3495
BseDI CCNNGG 7 cut(s) 114, 234, 876, 1528, 2006, 2898, 3524
BseJI GATNNNNATC 3 cut(s) 339, 924, 2826
BseLI CCNNNNNNNGG 8 cut(s) 36, 255, 354, 1115, 2678, 2852, 3067, 3130
BseMI GCAATG 4 cut(s) 1229, 1500, 2101, 3466
BseMII CTCAG 7 cut(s) 1268, 1767, 1779, 2041, 2079, 2318, 2745
BseNI ACTGG 6 cut(s) 323, 546, 771, 1056, 1338, 3292
BseRI GAGGAG 1 cut(s) 19
BseSI GKGCMC 1 cut(s) 2431
BseXI GCAGC 8 cut(s) 563, 744, 2162, 2390, 2427, 2781, 2808, 3319
Bsh1236I CGCG 2 cut(s) 689, 3366
Bsh1285I CGRYCG 1 cut(s) 2830
BshFI GGCC 5 cut(s) 72, 1608, 2043, 2657, 3123
BshNI GGYRCC 1 cut(s) 2255
BshTI ACCGGT 1 cut(s) 36
BshVI ATCGAT 4 cut(s) 625, 2826, 2830, 3495
BsiEI CGRYCG 1 cut(s) 2830
BsiHKAI GWGCWC 3 cut(s) 448, 683, 2571
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 6 cut(s) 37, 390, 968, 1200, 2654, 3061
BslFI GGGAC 1 cut(s) 3317
BslI CCNNNNNNNGG 8 cut(s) 36, 255, 354, 1115, 2678, 2852, 3067, 3130
BsmAI GTCTC 6 cut(s) 138, 220, 1479, 1766, 2004, 3107
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 3317
BsmI GAATGC 4 cut(s) 954, 2374, 2918, 3230
BsnI GGCC 5 cut(s) 72, 1608, 2043, 2657, 3123
Bso31I GGTCTC 1 cut(s) 2004
BsoBI CYCGRG 1 cut(s) 12
Bsp119I TTCGAA 1 cut(s) 1782
Bsp1286I GDGCHC 5 cut(s) 448, 683, 2431, 2571, 2696
Bsp13I TCCGGA 1 cut(s) 1199
Bsp19I CCATGG 2 cut(s) 114, 3524
Bsp68I TCGCGA 1 cut(s) 689
BspACI CCGC 5 cut(s) 706, 2372, 3146, 3228, 3514
BspANI GGCC 5 cut(s) 72, 1608, 2043, 2657, 3123
BspCNI CTCAG 7 cut(s) 1269, 1766, 1780, 2040, 2078, 2319, 2744
BspDI ATCGAT 4 cut(s) 625, 2826, 2830, 3495
BspEI TCCGGA 1 cut(s) 1199
BspFNI CGCG 2 cut(s) 689, 3366
BspHI TCATGA 3 cut(s) 1018, 1309, 2187
BspLI GGNNCC 3 cut(s) 342, 2257, 3419
BspOI GCTAGC 1 cut(s) 1629
BspQI GCTCTTC 1 cut(s) 436
BspT104I TTCGAA 1 cut(s) 1782
BspT107I GGYRCC 1 cut(s) 2255
BspTNI GGTCTC 1 cut(s) 2004
BsrDI GCAATG 4 cut(s) 1229, 1500, 2101, 3466
BsrFI RCCGGY 2 cut(s) 36, 2653
BsrI ACTGG 6 cut(s) 323, 546, 771, 1056, 1338, 3292
BssAI RCCGGY 2 cut(s) 36, 2653
BssECI CCNNGG 7 cut(s) 114, 234, 876, 1528, 2006, 2898, 3524
BssNAI GTATAC 1 cut(s) 838
BssNI GRCGYC 2 cut(s) 1245, 1702
BssT1I CCWWGG 4 cut(s) 114, 234, 2898, 3524
Bst1107I GTATAC 1 cut(s) 838
Bst2UI CCWGG 3 cut(s) 74, 1530, 1943
Bst4CI ACNGT 4 cut(s) 1154, 2852, 2989, 3451
Bst6I CTCTTC 2 cut(s) 436, 2007
BstACI GRCGYC 2 cut(s) 1245, 1702
BstBI TTCGAA 1 cut(s) 1782
BstC8I GCNNGC 5 cut(s) 123, 472, 788, 1089, 1627
BstDSI CCRYGG 3 cut(s) 114, 876, 3524
BstFNI CGCG 2 cut(s) 689, 3366
BstH2I RGCGCY 1 cut(s) 2896
BstHHI GCGC 2 cut(s) 2895, 2918
BstMAI GTCTC 6 cut(s) 138, 220, 1479, 1766, 2004, 3107
BstMCI CGRYCG 1 cut(s) 2830
BstMWI GCNNNNNNNGC 4 cut(s) 467, 958, 1727, 2566
BstNI CCWGG 3 cut(s) 74, 1530, 1943
BstNSI RCATGY 8 cut(s) 314, 613, 1091, 1142, 1960, 1982, 3048, 3388
BstSCI CCNGG 5 cut(s) 72, 967, 1528, 1941, 3059
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 2431
BstUI CGCG 2 cut(s) 689, 3366
BstV1I GCAGC 8 cut(s) 563, 744, 2162, 2390, 2427, 2781, 2808, 3319
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 4 cut(s) 340, 1187, 2207, 2218
BstYI RGATCY 4 cut(s) 340, 1187, 2207, 2218
BstZ17I GTATAC 1 cut(s) 838
Bsu15I ATCGAT 4 cut(s) 625, 2826, 2830, 3495
Bsu36I CCTNAGG 2 cut(s) 2045, 3125
BsuI GTATCC 1 cut(s) 2132
BsuRI GGCC 5 cut(s) 72, 1608, 2043, 2657, 3123
BsuTUI ATCGAT 4 cut(s) 625, 2826, 2830, 3495
BtgI CCRYGG 3 cut(s) 114, 876, 3524
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 4 cut(s) 440, 778, 1150, 2985
BtuMI TCGCGA 1 cut(s) 689
Cac8I GCNNGC 5 cut(s) 123, 472, 788, 1089, 1627
CaiI CAGNNNCTG 1 cut(s) 1760
CciI TCATGA 3 cut(s) 1018, 1309, 2187
CfoI GCGC 2 cut(s) 2895, 2918
Cfr10I RCCGGY 2 cut(s) 36, 2653
Cfr13I GGNCC 4 cut(s) 1606, 2863, 3121, 3407
ClaI ATCGAT 4 cut(s) 625, 2826, 2830, 3495
CpoI CGGWCCG 1 cut(s) 3407
CsiI ACCWGGT 1 cut(s) 1941
Csp6I GTAC 8 cut(s) 411, 1145, 1374, 1590, 1916, 2455, 2781, 3301
CspAI ACCGGT 1 cut(s) 36
CspCI CAANNNNNGTGG 4 cut(s) 1122, 1157, 2562, 2597
CspI CGGWCCG 1 cut(s) 3407
CviQI GTAC 8 cut(s) 411, 1145, 1374, 1590, 1916, 2455, 2781, 3301
DraI TTTAAA 1 cut(s) 3247
DrdI GACNNNNNNGTC 1 cut(s) 2810
DseDI GACNNNNNNGTC 1 cut(s) 2810
EaeI YGGCCR 1 cut(s) 2655
Eam1104I CTCTTC 2 cut(s) 436, 2007
EarI CTCTTC 2 cut(s) 436, 2007
EciI GGCGGA 1 cut(s) 3161
Ecl136II GAGCTC 2 cut(s) 446, 2569
Eco130I CCWWGG 4 cut(s) 114, 234, 2898, 3524
Eco24I GRGCYC 3 cut(s) 448, 2571, 2696
Eco31I GGTCTC 1 cut(s) 2004
Eco32I GATATC 2 cut(s) 811, 2824
Eco47I GGWCC 2 cut(s) 2863, 3407
Eco53kI GAGCTC 2 cut(s) 446, 2569
Eco57I CTGAAG 4 cut(s) 296, 399, 744, 1078
Eco81I CCTNAGG 2 cut(s) 2045, 3125
Eco88I CYCGRG 1 cut(s) 12
EcoICRI GAGCTC 2 cut(s) 446, 2569
EcoO109I RGGNCCY 2 cut(s) 1606, 3121
EcoRII CCWGG 3 cut(s) 72, 1528, 1941
EcoRV GATATC 2 cut(s) 811, 2824
EcoT14I CCWWGG 4 cut(s) 114, 234, 2898, 3524
EcoT22I ATGCAT 2 cut(s) 1473, 3046
EcoT38I GRGCYC 3 cut(s) 448, 2571, 2696
ErhI CCWWGG 4 cut(s) 114, 234, 2898, 3524
Esp3I CGTCTC 1 cut(s) 138
FalI AAGNNNNNCTT 2 cut(s) 927, 959
FaqI GGGAC 1 cut(s) 3317
FauI CCCGC 2 cut(s) 3221, 3521
FbaI TGATCA 3 cut(s) 97, 1306, 2278
FblI GTMKAC 2 cut(s) 307, 837
Fnu4HI GCNGC 8 cut(s) 552, 733, 2176, 2404, 2441, 2770, 2797, 3308
FriOI GRGCYC 3 cut(s) 448, 2571, 2696
Fsp4HI GCNGC 8 cut(s) 552, 733, 2176, 2404, 2441, 2770, 2797, 3308
FspBI CTAG 6 cut(s) 1424, 1439, 1626, 2144, 2211, 2756
GlaI GCGC 2 cut(s) 2894, 2917
GluI GCNGC 8 cut(s) 552, 733, 2176, 2404, 2441, 2770, 2797, 3308
HaeII RGCGCY 1 cut(s) 2896
HaeIII GGCC 5 cut(s) 72, 1608, 2043, 2657, 3123
HapII CCGG 6 cut(s) 37, 390, 968, 1200, 2654, 3061
HhaI GCGC 2 cut(s) 2895, 2918
Hin1I GRCGYC 2 cut(s) 1245, 1702
Hin6I GCGC 2 cut(s) 2893, 2916
HinP1I GCGC 2 cut(s) 2893, 2916
HincII GTYRAC 3 cut(s) 1429, 1840, 3447
HindII GTYRAC 3 cut(s) 1429, 1840, 3447
HindIII AAGCTT 3 cut(s) 2192, 2243, 2516
HpaII CCGG 6 cut(s) 37, 390, 968, 1200, 2654, 3061
HphI GGTGA 4 cut(s) 92, 1492, 1509, 2643
Hpy166II GTNNAC 9 cut(s) 308, 838, 1429, 1840, 2369, 2390, 2783, 3301, 3447
Hpy8I GTNNAC 9 cut(s) 308, 838, 1429, 1840, 2369, 2390, 2783, 3301, 3447
Hpy99I CGWCG 2 cut(s) 1704, 1707
HpyCH4III ACNGT 4 cut(s) 1154, 2852, 2989, 3451
HpyF10VI GCNNNNNNNGC 4 cut(s) 467, 958, 1727, 2566
Hsp92I GRCGYC 2 cut(s) 1245, 1702
HspAI GCGC 2 cut(s) 2893, 2916
Kpn2I TCCGGA 1 cut(s) 1199
Ksp22I TGATCA 3 cut(s) 97, 1306, 2278
LguI GCTCTTC 1 cut(s) 436
LmnI GCTCC 8 cut(s) 118, 400, 430, 571, 1126, 2574, 2900, 2973
Lsp1109I GCAGC 8 cut(s) 563, 744, 2162, 2390, 2427, 2781, 2808, 3319
LweI GCATC 7 cut(s) 470, 1424, 1480, 1549, 1652, 1772, 1794
MabI ACCWGGT 1 cut(s) 1941
MaeI CTAG 6 cut(s) 1424, 1439, 1626, 2144, 2211, 2756
MaeIII GTNAC 7 cut(s) 207, 615, 985, 1118, 1454, 2981, 3380
MfeI CAATTG 2 cut(s) 1475, 2588
MflI RGATCY 4 cut(s) 340, 1187, 2207, 2218
MhlI GDGCHC 5 cut(s) 448, 683, 2431, 2571, 2696
MlyI GAGTC 6 cut(s) 188, 215, 2064, 2138, 3297, 3503
MmeI TCCRAC 7 cut(s) 162, 559, 1362, 1624, 2082, 2430, 3385
Mph1103I ATGCAT 2 cut(s) 1473, 3046
MroI TCCGGA 1 cut(s) 1199
MroXI GAANNNNTTC 3 cut(s) 1503, 1690, 1863
MslI CAYNNNNRTG 2 cut(s) 1493, 2037
MspI CCGG 6 cut(s) 37, 390, 968, 1200, 2654, 3061
MspR9I CCNGG 5 cut(s) 74, 969, 1530, 1943, 3061
MunI CAATTG 2 cut(s) 1475, 2588
Mva1269I GAATGC 4 cut(s) 954, 2374, 2918, 3230
MvaI CCWGG 3 cut(s) 74, 1530, 1943
MvnI CGCG 2 cut(s) 689, 3366
MwoI GCNNNNNNNGC 4 cut(s) 467, 958, 1727, 2566
NciI CCSGG 2 cut(s) 969, 3061
NcoI CCATGG 2 cut(s) 114, 3524
NheI GCTAGC 1 cut(s) 1625
NlaIV GGNNCC 3 cut(s) 342, 2257, 3419
NmuCI GTSAC 1 cut(s) 207
NruI TCGCGA 1 cut(s) 689
NsiI ATGCAT 2 cut(s) 1473, 3046
NspI RCATGY 8 cut(s) 314, 613, 1091, 1142, 1960, 1982, 3048, 3388
NspV TTCGAA 1 cut(s) 1782
PaeI GCATGC 1 cut(s) 1091
PagI TCATGA 3 cut(s) 1018, 1309, 2187
PciI ACATGT 4 cut(s) 310, 609, 1138, 1978
PciSI GCTCTTC 1 cut(s) 436
PcsI WCGNNNNNNNCGW 2 cut(s) 693, 2832
PctI GAATGC 4 cut(s) 954, 2374, 2918, 3230
PdmI GAANNNNTTC 3 cut(s) 1503, 1690, 1863
PflFI GACNNNGTC 1 cut(s) 1701
PfoI TCCNGGA 1 cut(s) 967
PinAI ACCGGT 1 cut(s) 36
PkrI GCNGC 8 cut(s) 553, 734, 2177, 2405, 2442, 2771, 2798, 3309
Ple19I CGATCG 1 cut(s) 2830
PleI GAGTC 6 cut(s) 187, 214, 2064, 2137, 3296, 3503
PpsI GAGTC 6 cut(s) 187, 214, 2064, 2137, 3296, 3503
PscI ACATGT 4 cut(s) 310, 609, 1138, 1978
PsiI TTATAA 1 cut(s) 702
Psp124BI GAGCTC 2 cut(s) 448, 2571
Psp1406I AACGTT 1 cut(s) 1637
Psp6I CCWGG 3 cut(s) 72, 1528, 1941
PspGI CCWGG 3 cut(s) 72, 1528, 1941
PspN4I GGNNCC 3 cut(s) 342, 2257, 3419
PspPI GGNCC 4 cut(s) 1606, 2863, 3121, 3407
PstNI CAGNNNCTG 1 cut(s) 1760
PsuI RGATCY 4 cut(s) 340, 1187, 2207, 2218
PsyI GACNNNGTC 1 cut(s) 1701
PvuI CGATCG 1 cut(s) 2830
RruI TCGCGA 1 cut(s) 689
RsaI GTAC 8 cut(s) 412, 1146, 1375, 1591, 1917, 2456, 2782, 3302
RsaNI GTAC 8 cut(s) 411, 1145, 1374, 1590, 1916, 2455, 2781, 3301
RseI CAYNNNNRTG 2 cut(s) 1493, 2037
Rsr2I CGGWCCG 1 cut(s) 3407
RsrII CGGWCCG 1 cut(s) 3407
SacI GAGCTC 2 cut(s) 448, 2571
SapI GCTCTTC 1 cut(s) 436
SatI GCNGC 8 cut(s) 552, 733, 2176, 2404, 2441, 2770, 2797, 3308
Sau96I GGNCC 4 cut(s) 1606, 2863, 3121, 3407
ScaI AGTACT 1 cut(s) 2456
SchI GAGTC 6 cut(s) 188, 215, 2064, 2138, 3297, 3503
ScrFI CCNGG 5 cut(s) 74, 969, 1530, 1943, 3061
SduI GDGCHC 5 cut(s) 448, 683, 2431, 2571, 2696
SexAI ACCWGGT 1 cut(s) 1941
SfaNI GCATC 7 cut(s) 470, 1424, 1480, 1549, 1652, 1772, 1794
SfcI CTRYAG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 1782
SinI GGWCC 2 cut(s) 2863, 3407
SmiMI CAYNNNNRTG 2 cut(s) 1493, 2037
SmlI CTYRAG 6 cut(s) 58, 250, 1070, 2081, 2246, 3500
SmoI CTYRAG 6 cut(s) 58, 250, 1070, 2081, 2246, 3500
SpeI ACTAGT 1 cut(s) 2755
SphI GCATGC 1 cut(s) 1091
SsiI CCGC 5 cut(s) 706, 2372, 3146, 3228, 3514
SspI AATATT 4 cut(s) 175, 1570, 1600, 2338
SspMI CTAG 6 cut(s) 1424, 1439, 1626, 2144, 2211, 2756
SstI GAGCTC 2 cut(s) 448, 2571
StyD4I CCNGG 5 cut(s) 72, 967, 1528, 1941, 3059
StyI CCWWGG 4 cut(s) 114, 234, 2898, 3524
TaaI ACNGT 4 cut(s) 1154, 2852, 2989, 3451
TaqII GACCGA 1 cut(s) 3395
TatI WGTACW 2 cut(s) 1373, 2454
TscAI CASTG 4 cut(s) 440, 778, 1157, 2992
TseFI GTSAC 1 cut(s) 207
TseI GCWGC 8 cut(s) 551, 732, 2175, 2403, 2440, 2769, 2796, 3307
Tsp45I GTSAC 1 cut(s) 207
TspGWI ACGGA 3 cut(s) 428, 1607, 2768
TspRI CASTG 4 cut(s) 440, 778, 1157, 2992
Tth111I GACNNNGTC 1 cut(s) 1701
VpaK11BI GGWCC 2 cut(s) 2863, 3407
XapI RAATTY 8 cut(s) 1263, 1318, 1446, 1862, 1924, 2016, 2110, 2629
XceI RCATGY 8 cut(s) 314, 613, 1091, 1142, 1960, 1982, 3048, 3388
XmiI GTMKAC 2 cut(s) 307, 837
XmnI GAANNNNTTC 3 cut(s) 1503, 1690, 1863
XspI CTAG 6 cut(s) 1424, 1439, 1626, 2144, 2211, 2756
ZraI GACGTC 2 cut(s) 1246, 1703
ZrmI AGTACT 1 cut(s) 2456
Zsp2I ATGCAT 2 cut(s) 1473, 3046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.