pycom17g27360

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000654
Physical Location & Seq
Forward (+)
25585719 .. 25586689
971 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
ATGCTGTCCTTGTTAGAGTACTTGAATCTGTATGGTAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCGGACTCTTAATGTATCTGGTTGCAGAAACCTCACAACACTTGCAGAGCTCCCACGTTCTTTGACAAGTTTAAGGCTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTCGTTAGAGTACTTGTCTCTGCATAAGAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTGAAGTATCTGGTTGCAGAAACCTCACAATGCTTCCAGAGCTCCCACGTAGTGTGACACAATTGTATGGTCTAGGTTGCACATCATTGAAAAGAATAACAAATTCACCGAGGTCACCGGACTTAGATTACGGGTCTTCTAGCATAGTAGTGGACCCAAGGCTATGGTATTGTGGGAAACTTGTTGATTTTGGAAGCTTGTTGGATACACTAGCATTGGACTCATATCTCTCATATCTATTCGATTTGGAACCCAAAGGTGGCATTGAGGTCCAAGGGAACTGTTGTGAGAGTTTAGGCGTCCAGGGAACTTTTAGCTGCTGCATATTGAGCATATTTGTTCATGGAAGCAAGATTCCAGATGGGTTCAATTACAGGAGAATGGGTAACACTGTGTTCTCTATGATTGTTCCTTCACATCTTAATCTCAAGATCGTAGGTTTAAACGCATGTGTATTATATGCACGGCAGTCGAATCGGAGCCTTGAGGTTCCATGTGTGGAAATGGCGTGTGTGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

271

Amino Acids

29.63

Weight (kDa)

6.79

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 2 - 76 1.2e-07 Leucine-rich repeat region
LRR_4 PF12799 5 - 46 2.1e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 47 - 104 3.2e-09 Leucine rich repeat
LRR_4 PF12799 48 - 86 4.7e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 66 - 134 1.4e-08 Leucine-rich repeat region
LRR_4 PF12799 71 - 113 3.7e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 84
AcsI RAATTY 1 cut(s) 394
AcyI GRCGYC 1 cut(s) 591
AdeI CACNNNGTG 1 cut(s) 344
AfaI GTAC 2 cut(s) 20, 221
AfiI CCNNNNNNNGG 1 cut(s) 551
AgsI TTSAA 4 cut(s) 25, 296, 382, 661
AjnI CCWGG 1 cut(s) 594
AluBI AGCT 6 cut(s) 82, 133, 283, 334, 489, 609
AluI AGCT 6 cut(s) 82, 133, 283, 334, 489, 609
Alw21I GWGCWC 2 cut(s) 135, 336
Alw26I GTCTC 1 cut(s) 231
Aor13HI TCCGGA 1 cut(s) 84
ApeKI GCWGC 3 cut(s) 168, 609, 612
ApoI RAATTY 1 cut(s) 394
AspS9I GGNCC 2 cut(s) 445, 562
AsuHPI GGTGA 2 cut(s) 390, 399
AvaII GGWCC 2 cut(s) 445, 562
BaeGI GKGCMC 1 cut(s) 196
BanII GRGCYC 2 cut(s) 135, 336
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 135, 336
BbvI GCAGC 3 cut(s) 155, 596, 599
BccI CCATC 1 cut(s) 647
BceAI ACGGC 1 cut(s) 773
BcgI CGANNNNNNTGC 4 cut(s) 744, 749, 778, 783
BciT130I CCWGG 1 cut(s) 596
BciVI GTATCC 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 3 cut(s) 365, 432, 503
BfuI GTATCC 1 cut(s) 490
BisI GCNGC 3 cut(s) 169, 610, 613
BlsI GCNGC 3 cut(s) 170, 611, 614
BmcAI AGTACT 2 cut(s) 20, 221
Bme1390I CCNGG 1 cut(s) 596
Bme18I GGWCC 2 cut(s) 445, 562
BmgT120I GGNCC 2 cut(s) 445, 562
BmiI GGNNCC 4 cut(s) 447, 543, 773, 783
BmrFI CCNGG 1 cut(s) 596
BpiI GAAGAC 1 cut(s) 420
BpmI CTGGAG 1 cut(s) 269
BpuEI CTTGAG 2 cut(s) 704, 797
BsaAI YACGTR 1 cut(s) 341
BsaHI GRCGYC 1 cut(s) 591
BsaJI CCNNGG 4 cut(s) 401, 449, 565, 595
BsaWI WCCGGW 2 cut(s) 84, 409
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bsc4I CCNNNNNNNGG 1 cut(s) 551
BseAI TCCGGA 1 cut(s) 84
BseBI CCWGG 1 cut(s) 596
BseDI CCNNGG 4 cut(s) 401, 449, 565, 595
BseLI CCNNNNNNNGG 1 cut(s) 551
BseSI GKGCMC 1 cut(s) 196
BseXI GCAGC 3 cut(s) 155, 596, 599
BsiHKAI GWGCWC 2 cut(s) 135, 336
BsiSI CCGG 2 cut(s) 85, 410
BslI CCNNNNNNNGG 1 cut(s) 551
BsmAI GTCTC 1 cut(s) 231
Bsp1286I GDGCHC 3 cut(s) 135, 196, 336
Bsp13I TCCGGA 1 cut(s) 84
Bsp143I GATC 1 cut(s) 723
BspEI TCCGGA 1 cut(s) 84
BspLI GGNNCC 4 cut(s) 447, 543, 773, 783
BssECI CCNNGG 4 cut(s) 401, 449, 565, 595
BssMI GATC 1 cut(s) 723
BssNI GRCGYC 1 cut(s) 591
BssT1I CCWWGG 2 cut(s) 449, 565
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 2 cut(s) 575, 685
BstACI GRCGYC 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 341
BstC8I GCNNGC 2 cut(s) 162, 166
BstDEI CTNAG 1 cut(s) 415
BstEII GGTNACC 1 cut(s) 405
BstKTI GATC 1 cut(s) 726
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 1 cut(s) 723
BstMWI GCNNNNNNNGC 3 cut(s) 331, 621, 806
BstNI CCWGG 1 cut(s) 596
BstNSI RCATGY 1 cut(s) 744
BstPI GGTNACC 1 cut(s) 405
BstSCI CCNGG 1 cut(s) 594
BstSLI GKGCMC 1 cut(s) 196
BstV1I GCAGC 3 cut(s) 155, 596, 599
BstV2I GAAGAC 1 cut(s) 420
BstXI CCANNNNNNTGG 1 cut(s) 456
BsuI GTATCC 1 cut(s) 490
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 2 cut(s) 162, 166
Cfr13I GGNCC 2 cut(s) 445, 562
CseI GACGC 1 cut(s) 580
Csp6I GTAC 2 cut(s) 19, 220
CspCI CAANNNNNGTGG 2 cut(s) 126, 161
CviAII CATG 5 cut(s) 64, 265, 635, 741, 786
CviQI GTAC 2 cut(s) 19, 220
DdeI CTNAG 1 cut(s) 415
DpnI GATC 1 cut(s) 725
DpnII GATC 1 cut(s) 723
DraI TTTAAA 1 cut(s) 735
DraIII CACNNNGTG 1 cut(s) 344
Ecl136II GAGCTC 2 cut(s) 133, 334
Eco130I CCWWGG 2 cut(s) 449, 565
Eco24I GRGCYC 2 cut(s) 135, 336
Eco47I GGWCC 2 cut(s) 445, 562
Eco53kI GAGCTC 2 cut(s) 133, 334
Eco91I GGTNACC 1 cut(s) 405
EcoICRI GAGCTC 2 cut(s) 133, 334
EcoO65I GGTNACC 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 594
EcoT14I CCWWGG 2 cut(s) 449, 565
EcoT38I GRGCYC 2 cut(s) 135, 336
ErhI CCWWGG 2 cut(s) 449, 565
FaeI CATG 5 cut(s) 67, 268, 638, 744, 789
FalI AAGNNNNNCTT 2 cut(s) 148, 180
FatI CATG 5 cut(s) 63, 264, 634, 740, 785
Fnu4HI GCNGC 3 cut(s) 169, 610, 613
FriOI GRGCYC 2 cut(s) 135, 336
Fsp4HI GCNGC 3 cut(s) 169, 610, 613
FspBI CTAG 3 cut(s) 365, 432, 503
GluI GCNGC 3 cut(s) 169, 610, 613
GsuI CTGGAG 1 cut(s) 269
HapII CCGG 2 cut(s) 85, 410
HgaI GACGC 1 cut(s) 580
Hin1I GRCGYC 1 cut(s) 591
Hin1II CATG 5 cut(s) 67, 268, 638, 744, 789
HindIII AAGCTT 1 cut(s) 487
HinfI GANTC 8 cut(s) 25, 88, 184, 238, 289, 512, 646, 766
HpaII CCGG 2 cut(s) 85, 410
HphI GGTGA 2 cut(s) 390, 399
Hpy166II GTNNAC 1 cut(s) 445
Hpy188I TCNGA 4 cut(s) 42, 181, 243, 771
Hpy188III TCNNGA 6 cut(s) 85, 286, 293, 329, 650, 721
Hpy8I GTNNAC 1 cut(s) 445
HpyAV CCTTC 1 cut(s) 714
HpyCH4III ACNGT 2 cut(s) 575, 685
HpyCH4IV ACGT 2 cut(s) 139, 340
HpyCH4V TGCA 8 cut(s) 108, 128, 171, 232, 309, 372, 615, 755
HpyF10VI GCNNNNNNNGC 3 cut(s) 331, 621, 806
HpyF3I CTNAG 1 cut(s) 415
HpySE526I ACGT 2 cut(s) 139, 340
Hsp92I GRCGYC 1 cut(s) 591
Hsp92II CATG 5 cut(s) 67, 268, 638, 744, 789
Kpn2I TCCGGA 1 cut(s) 84
Kzo9I GATC 1 cut(s) 723
LmnI GCTCC 5 cut(s) 87, 138, 288, 339, 771
Lsp1109I GCAGC 3 cut(s) 155, 596, 599
MaeI CTAG 3 cut(s) 365, 432, 503
MaeII ACGT 2 cut(s) 139, 340
MaeIII GTNAC 3 cut(s) 346, 405, 677
MalI GATC 1 cut(s) 725
MboI GATC 1 cut(s) 723
MboII GAAGA 1 cut(s) 420
MfeI CAATTG 1 cut(s) 353
MhlI GDGCHC 3 cut(s) 135, 196, 336
MluCI AATT 3 cut(s) 353, 394, 661
MlyI GAGTC 3 cut(s) 82, 283, 506
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 6 cut(s) 125, 220, 326, 396, 553, 772
MroI TCCGGA 1 cut(s) 84
MseI TTAA 6 cut(s) 78, 93, 155, 279, 714, 734
MslI CAYNNNNRTG 1 cut(s) 440
MspI CCGG 2 cut(s) 85, 410
MspR9I CCNGG 1 cut(s) 596
MssI GTTTAAAC 1 cut(s) 735
MunI CAATTG 1 cut(s) 353
MvaI CCWGG 1 cut(s) 596
MwoI GCNNNNNNNGC 3 cut(s) 331, 621, 806
NdeII GATC 1 cut(s) 723
NlaIII CATG 5 cut(s) 67, 268, 638, 744, 789
NlaIV GGNNCC 4 cut(s) 447, 543, 773, 783
NmuCI GTSAC 2 cut(s) 346, 405
NspI RCATGY 1 cut(s) 744
PfeI GAWTC 5 cut(s) 25, 184, 238, 646, 766
PkrI GCNGC 3 cut(s) 170, 611, 614
PleI GAGTC 3 cut(s) 82, 283, 506
PmeI GTTTAAAC 1 cut(s) 735
PpsI GAGTC 3 cut(s) 82, 283, 506
Ppu21I YACGTR 1 cut(s) 341
Psp124BI GAGCTC 2 cut(s) 135, 336
Psp6I CCWGG 1 cut(s) 594
PspEI GGTNACC 1 cut(s) 405
PspGI CCWGG 1 cut(s) 594
PspN4I GGNNCC 4 cut(s) 447, 543, 773, 783
PspPI GGNCC 2 cut(s) 445, 562
RsaI GTAC 2 cut(s) 20, 221
RsaNI GTAC 2 cut(s) 19, 220
RseI CAYNNNNRTG 1 cut(s) 440
SacI GAGCTC 2 cut(s) 135, 336
SaqAI TTAA 6 cut(s) 78, 93, 155, 279, 714, 734
SatI GCNGC 3 cut(s) 169, 610, 613
Sau3AI GATC 1 cut(s) 723
Sau96I GGNCC 2 cut(s) 445, 562
ScaI AGTACT 2 cut(s) 20, 221
SchI GAGTC 3 cut(s) 82, 283, 506
ScrFI CCNGG 1 cut(s) 596
SduI GDGCHC 3 cut(s) 135, 196, 336
SinI GGWCC 2 cut(s) 445, 562
SmiMI CAYNNNNRTG 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 719, 776
SmoI CTYRAG 2 cut(s) 719, 776
Sse9I AATT 3 cut(s) 353, 394, 661
SspMI CTAG 3 cut(s) 365, 432, 503
SstI GAGCTC 2 cut(s) 135, 336
StyD4I CCNGG 1 cut(s) 594
StyI CCWWGG 2 cut(s) 449, 565
TaaI ACNGT 2 cut(s) 575, 685
TaiI ACGT 2 cut(s) 142, 343
TaqI TCGA 2 cut(s) 534, 764
TasI AATT 3 cut(s) 353, 394, 661
TatI WGTACW 2 cut(s) 18, 219
TfiI GAWTC 5 cut(s) 25, 184, 238, 646, 766
Tru1I TTAA 6 cut(s) 78, 93, 155, 279, 714, 734
Tru9I TTAA 6 cut(s) 78, 93, 155, 279, 714, 734
TscAI CASTG 1 cut(s) 688
TseFI GTSAC 2 cut(s) 346, 405
TseI GCWGC 3 cut(s) 168, 609, 612
Tsp45I GTSAC 2 cut(s) 346, 405
TspDTI ATGAA 3 cut(s) 80, 281, 623
TspRI CASTG 1 cut(s) 688
VpaK11BI GGWCC 2 cut(s) 445, 562
XapI RAATTY 1 cut(s) 394
XceI RCATGY 1 cut(s) 744
XspI CTAG 3 cut(s) 365, 432, 503
ZrmI AGTACT 2 cut(s) 20, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.