pycom17g27450

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000654
Physical Location & Seq
Forward (+)
25617111 .. 25623397
6287 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3537 bp
ATGGGAACCTTCCTCTTCATATATATGTCCCTCTCAGAAACTGCATATAATTGTCCTCGGCCTTGCATATTCGTGCCCTGCATTCAGTCATTGCAAGAACCAAGAAGCAGAGAGTTTCTCTATATGGATGCTTTCCGAGATTCTCATTCGTCCTCTTACCGGTGTTCCTATCATGCCTTCTTGAGTTTCAGAGGCCAGGACACGCGCAAGGGCTTTACTGATCACCTCTATAGAGCCTTGGAGCTAGCAGGAATCCACACGTTTAGAGACGATGATGAAATCAAGAGAGGGGAGAAGATTGAGTCGGAGTTAGAGAAGGCGATACAGGAGTCACAAGTATCGATAATTGTCTTCTCCAAGGACTATGCTTCCTCAAGGTGGTGTTTGAACGAACTTTTGAAGATCGTTGGACGTAGAAATACTGATCTTAGACATGTGGTTCTGCCAGTTTTCTATGATGTGGATCCATCCGATGTCAGGAAGCAGAGTGGTTCTTTTGTTGAAGCATTTGCCAGACATGAGAAGCGATTCAGTACGGAGGAGATGGACAAGGTGGAGCTGTGGAGAAGAGCTCTTAGAGATGTTGCGTCTTTGGGAGGAATGGTTTTAGGAGATCGGTATGAGGGGCAGTTCATCCAAGATATTGTTGAAGAGATTAGAAATAAACTGGATCACGCAGCTTTAGACGTCGCTCCCTTTTCAGTTGGAATCGATAATCGTGTGAGACGCCTAAACATGTGGTTACAAGATGGATCAAATGATGTTGGTGTAGCTGTCATCTGTGGGATGGGTGGAATTGGCAAGAGCACTATCGCGAAGGCCGCTTATAACCGCAACTTTGATAGATTTCAAGGTAGCAGCTTTCTTGCAGATATACGAGAATCTTCAGAACATGGTTTTGTTGGCTTGCAAAGAAAACTTCTTTCAGATATCCAAGAGGGGAAAGCAAAGAAAGTATACAATACGGATGAAGGAACAATCAGGATCGATCAACCCGTGGGCTATAAAAAAGTTCTTATTGTTCTTGATGATGTGAGCAACCGAGATCAACTCAATGCAATTCTTGGAATGCGAGAGTGGCTTCATCCAGGAAGTAAAATTATCATAACAACTCGACATGAACATTTGTTGAATGCTCACGATGTTTTTGAAAAGTTCATGGTTCCAGAACTGAATGAGTACGAATCACTTGAGCTTTTCAGTTGGCATGCCTTCAGACAATCCCATCCAGAAGAAGGTTACATGGAGCTTTCAAGACATGTGGTACAGCACTGTGGAGGGCTTCCATTAGCTCTTCAAGTTCTGGGATCTTCTCTATCCGGAAAAAGTGAAGAAGTATGGCAAAGTGCATTGCAGAAGTTAGACGTGATCCCTAATGACAAAATTCAAAAAGTTCTGAGGATAAGTTTTGATTCTTTACAAGATGATCATGACAAAAATTTATTCCTTCACATAGCCAGTTTCTTCACAGGAAAGAAGATGGATTATACGAGTACAATGTTGGACAGCTTAGAGTTTTACACAAGGATTGGGATTGAAAATCTAGTTGACAGATGCCTAGTGAAAATACAATGGCCGAACAGGTTGGTCATGCATCAATTGGTGAGAAACATGGCAATGGAAATTATTCGTGAGGAATCACCTGATGACCCCGGAAAACGTACTAGAGTGTTGCAGAAAGATGCCTCTAATATTTTGAGAAAATTAAGTGGTACGGAAAATGTTAAGGGCCTCATGCTCAACCTTGCTTGCAAAAGAACGTTAGTTGGAAGTGATAAACAACGCTGCCATGTTGAAGATTATGATGAAAATTCTTCAAGACGAAGTGGGCTTGGTTTCTTCTCTTGGAAACCAATTAGCTTTTCATCCACAAACTCAGCTTCTGCATCAAATGAGATAGATTTCAAACCTGAGGCGTTTAGAAGGATGTACAATCTTGAAATTCTCATGCTCAACAACGTCAACGTCAGGGGAAGCTACGAAGAATTTCCAAAAAGTTTAATATGTTTATCTTGGAGAGGATTCCACTTAAAATCAGTACCAGAAAATTTTTATTTGGAAAACCTGGTTGTTCTTGACATGCGAAATAGTAGCCTACAACATGTTTGGAATGGGATCAGGTTTCTTCCGAGGCTGAAAATTCTTAATCTCAGTCATTCGCATGGCCTTAGGACAACCCCCGACTTCTCAGGACTCCCTAACCTTGAGAGATTGATTCTTAAACATTGCATAAATTTGGTTGAGGTTGATGAGTCCATCGGATACCTAGAGAAACTTCTTGTTTTGAATCTACAAGGCTGCAAAAATCTCATGAAGCTTCCAATATTGAGATCTGTGCGGGATCTTGATCTTTCTGGTTGCTCAAAGCTTGTGCTTGGTGCCAATACGACTGCCACAACTGATCACTTACAATCTACAGCTTGTGAAATGAAGAAGCTCAATCTGTTATCACCTAAATTATGGTATTCAATCTGGTCGTCGGTGTCGCCGAGAAAAAATATTGAATCAAGCAGTTTCTCATTGGCAAGTTTACCGCATTCCTTAACAAGTTTACGTCTGGATTGCTGCAATCTATCAGAGATTCCGAGTGCCCTTACTATGCTGCCTTCGTTAGAGTACTTGGATCTGAATAACAATCCAATTACAAGCCTACCAGAGAGCATGAACAATCTTGTTAAGCTTCGCACTCTTGCAGTAGAAGGTTGCAGAAACCTCACAATGCTTCCAGAGCTCCCACATGGTTTGAAACAATTGAATGGTAGAGGTTGCACATCATTGAAAAGAATAACAAATTTACCGAACTTGGACATATCACCGGCCCCCGATCCCTACTCTTGCGACATGTTATCGGACCCAGATTTAGAGTGTTACAACATAGTATTGGACTCAAATTTATGGTATTGTGGGAAACTAGTTGATGTTGCAAGCTTGTTCAATACACTAACATTGAGCAGCTTTGACATAAAACTCCTCAAAGATATCGATCTATTCGATTTGTTCGATTTGAGACCCAACGGTGGCATTGAGGTCGAAGGGAACAACTATTGTGCAAGTAGGCGCTCCAAGATTGCAGGCGTCCATTCGCTTGAACTTAGGAATGAGACAAAGGGCCTTAAGTGGAAATTAAGAATGTGGACAGAGGTTGAATGGAAAGTGATGAACGAAGATATGTTATGGCTAAGCCATTGGATTATGGGGAACAATGAATTGGAATGCGGGGAAGAAGTGTGTTTTAAAATCAGAAAGCAAGACGGTTTCGTCACAAAGGAGATTGGAGTCCAGTTTGTGTACGAGCAGCAAAATAAGGATAAGGAGGATGTCCCATCAAGTAGTGAAGATGCATTGATCTCACGTGAAATATCGGCTTGGGAGAATGAATCTTGGGAGAATGAATCGCTTTATTTGATAGGAGCGGACTTCTTACGCTTTATATCATGGATCCGTAACTGCGAGAATGAATCTCTGTCAACTGTCATCTGCAATCTCTGTAACCATGAAGTGATCGATTTTGAGAATGACTCCCGCATGACCCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1179

Amino Acids

134.76

Weight (kDa)

5.89

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 57 - 226 9.1e-50 TIR domain
TIR_2 PF13676 60 - 157 9.1e-16 TIR domain
NB-ARC PF00931 239 - 408 1.2e-17 NB-ARC domain
WHD_ROQ1 PF23282 476 - 547 1.3e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_8 PF13855 847 - 902 6.6e-09 Leucine rich repeat
LRR_4 PF12799 847 - 885 2e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 848 - 937 4e-07 Leucine-rich repeat region
LRR_4 PF12799 869 - 913 2e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
AasI GACNNNNNNGTC 1 cut(s) 3257
AatII GACGTC 1 cut(s) 690
AccB1I GGYRCC 1 cut(s) 2378
AccBSI CCGCTC 1 cut(s) 3413
AccI GTMKAC 1 cut(s) 957
AccII CGCG 2 cut(s) 205, 815
AccIII TCCGGA 1 cut(s) 1319
AciI CCGC 7 cut(s) 822, 832, 2338, 2534, 3216, 3413, 3523
AclI AACGTT 1 cut(s) 1760
AcoI YGGCCR 1 cut(s) 1574
AcuI CTGAAG 2 cut(s) 870, 1198
AcvI CACGTG 1 cut(s) 3353
AcyI GRCGYC 3 cut(s) 687, 727, 3075
AfiI CCNNNNNNNGG 5 cut(s) 159, 378, 477, 1235, 3017
AflII CTTAAG 1 cut(s) 3113
AflIII ACRYGT 6 cut(s) 258, 433, 735, 1258, 2101, 2841
AgeI ACCGGT 1 cut(s) 159
AhlI ACTAGT 1 cut(s) 2911
AjiI CACGTC 1 cut(s) 1366
AjnI CCWGG 3 cut(s) 195, 1087, 2064
AjuI GAANNNNNNNTTGG 4 cut(s) 1846, 1878, 3191, 3223
Alw21I GWGCWC 3 cut(s) 574, 809, 2733
Alw26I GTCTC 4 cut(s) 261, 718, 3001, 3095
AlwNI CAGNNNCTG 2 cut(s) 41, 1883
Aor13HI TCCGGA 1 cut(s) 1319
AoxI GGCC 8 cut(s) 59, 193, 819, 1574, 1729, 2164, 2817, 3109
ApeKI GCWGC 8 cut(s) 677, 858, 1785, 2298, 2565, 2602, 2952, 3295
AsiGI ACCGGT 1 cut(s) 159
Asp700I GAANNNNTTC 3 cut(s) 527, 1626, 1986
AspLEI GCGC 2 cut(s) 207, 3060
AspS9I GGNCC 4 cut(s) 1729, 2818, 2851, 3109
AsuC2I CCSGG 1 cut(s) 1653
AsuHPI GGTGA 5 cut(s) 215, 1615, 1632, 2442, 2805
AsuNHI GCTAGC 1 cut(s) 244
AvaII GGWCC 1 cut(s) 2851
AxyI CCTNAGG 2 cut(s) 1911, 2168
BaeGI GKGCMC 2 cut(s) 78, 2593
BaeI ACNNNNGTAYC 2 cut(s) 314, 347
BamHI GGATCC 2 cut(s) 463, 3438
BanI GGYRCC 1 cut(s) 2378
BanII GRGCYC 2 cut(s) 574, 2733
BbrPI CACGTG 1 cut(s) 3353
BbsI GAAGAC 1 cut(s) 343
Bbv12I GWGCWC 3 cut(s) 574, 809, 2733
BbvI GCAGC 8 cut(s) 689, 870, 1772, 2285, 2552, 2589, 2964, 3307
BccI CCATC 8 cut(s) 475, 538, 743, 781, 1233, 1474, 2264, 3331
BcgI CGANNNNNNTGC 2 cut(s) 3010, 3044
BciT130I CCWGG 3 cut(s) 197, 1089, 2066
BciVI GTATCC 1 cut(s) 2255
BclI TGATCA 3 cut(s) 220, 1426, 2401
BcnI CCSGG 1 cut(s) 1653
BcoDI GTCTC 4 cut(s) 261, 718, 3001, 3095
BcuI ACTAGT 1 cut(s) 2911
BfaI CTAG 6 cut(s) 245, 1544, 1559, 1665, 2267, 2912
BfmI CTRYAG 2 cut(s) 229, 2415
BfoI RGCGCY 1 cut(s) 3061
BfrI CTTAAG 1 cut(s) 3113
BfuI GTATCC 1 cut(s) 2255
BglII AGATCT 1 cut(s) 2330
BisI GCNGC 9 cut(s) 678, 822, 859, 1786, 2299, 2566, 2603, 2953, 3296
BlpI GCTNAGC 1 cut(s) 3179
BlsI GCNGC 9 cut(s) 679, 823, 860, 1787, 2300, 2567, 2604, 2954, 3297
BmcAI AGTACT 1 cut(s) 2618
Bme1390I CCNGG 4 cut(s) 197, 1089, 1653, 2066
Bme18I GGWCC 1 cut(s) 2851
BmgBI CACGTC 1 cut(s) 1366
BmgT120I GGNCC 4 cut(s) 1729, 2818, 2851, 3109
BmiI GGNNCC 7 cut(s) 7, 465, 1164, 2380, 2820, 2853, 3440
BmrFI CCNGG 4 cut(s) 197, 1089, 1653, 2066
BmsI GCATC 6 cut(s) 118, 1544, 1603, 1672, 1895, 3328
BmtI GCTAGC 1 cut(s) 248
BpiI GAAGAC 1 cut(s) 343
BplI GAGNNNNNCTC 8 cut(s) 102, 134, 556, 588, 1035, 1067, 3503, 3535
Bpu1102I GCTNAGC 1 cut(s) 3179
BpuEI CTTGAG 4 cut(s) 202, 358, 1211, 2225
BpuMI CCSGG 1 cut(s) 1653
Bsa29I ATCGAT 5 cut(s) 341, 711, 987, 2982, 3504
BsaAI YACGTR 1 cut(s) 3353
BsaBI GATNNNNATC 3 cut(s) 462, 2346, 2982
BsaHI GRCGYC 3 cut(s) 687, 727, 3075
BsaI GGTCTC 1 cut(s) 3001
BsaJI CCNNGG 6 cut(s) 56, 237, 357, 996, 1651, 2129
BsaWI WCCGGW 2 cut(s) 159, 1319
BsaXI ACNNNNNCTCC 2 cut(s) 588, 618
Bsc4I CCNNNNNNNGG 5 cut(s) 159, 378, 477, 1235, 3017
Bse118I RCCGGY 2 cut(s) 159, 2815
Bse1I ACTGG 4 cut(s) 446, 672, 1458, 3280
Bse21I CCTNAGG 2 cut(s) 1911, 2168
Bse3DI GCAATG 4 cut(s) 89, 1349, 1623, 2224
Bse8I GATNNNNATC 3 cut(s) 462, 2346, 2982
BseAI TCCGGA 1 cut(s) 1319
BseBI CCWGG 3 cut(s) 197, 1089, 2066
BseCI ATCGAT 5 cut(s) 341, 711, 987, 2982, 3504
BseDI CCNNGG 6 cut(s) 56, 237, 357, 996, 1651, 2129
BseJI GATNNNNATC 3 cut(s) 462, 2346, 2982
BseLI CCNNNNNNNGG 5 cut(s) 159, 378, 477, 1235, 3017
BseMI GCAATG 4 cut(s) 89, 1349, 1623, 2224
BseMII CTCAG 6 cut(s) 48, 1388, 1890, 1902, 2164, 2202
BseNI ACTGG 4 cut(s) 446, 672, 1458, 3280
BseRI GAGGAG 2 cut(s) 554, 2960
BseSI GKGCMC 2 cut(s) 78, 2593
BseXI GCAGC 8 cut(s) 689, 870, 1772, 2285, 2552, 2589, 2964, 3307
Bsh1236I CGCG 2 cut(s) 205, 815
BshFI GGCC 8 cut(s) 61, 195, 821, 1576, 1731, 2166, 2819, 3111
BshNI GGYRCC 1 cut(s) 2378
BshTI ACCGGT 1 cut(s) 159
BshVI ATCGAT 5 cut(s) 341, 711, 987, 2982, 3504
BsiHKAI GWGCWC 3 cut(s) 574, 809, 2733
BsiSI CCGG 4 cut(s) 160, 1320, 1653, 2816
BslFI GGGAC 2 cut(s) 13, 3305
BslI CCNNNNNNNGG 5 cut(s) 159, 378, 477, 1235, 3017
BsmAI GTCTC 4 cut(s) 261, 718, 3001, 3095
BsmBI CGTCTC 2 cut(s) 261, 718
BsmFI GGGAC 2 cut(s) 13, 3305
BsmI GAATGC 5 cut(s) 81, 1074, 1138, 2536, 3218
BsnI GGCC 8 cut(s) 61, 195, 821, 1576, 1731, 2166, 2819, 3111
Bso31I GGTCTC 1 cut(s) 3001
Bsp1286I GDGCHC 5 cut(s) 78, 574, 809, 2593, 2733
Bsp13I TCCGGA 1 cut(s) 1319
Bsp1407I TGTACA 1 cut(s) 1929
Bsp1720I GCTNAGC 1 cut(s) 3179
Bsp68I TCGCGA 1 cut(s) 815
BspACI CCGC 7 cut(s) 822, 832, 2338, 2534, 3216, 3413, 3523
BspANI GGCC 8 cut(s) 61, 195, 821, 1576, 1731, 2166, 2819, 3111
BspCNI CTCAG 6 cut(s) 47, 1389, 1889, 1903, 2163, 2201
BspDI ATCGAT 5 cut(s) 341, 711, 987, 2982, 3504
BspEI TCCGGA 1 cut(s) 1319
BspFNI CGCG 2 cut(s) 205, 815
BspHI TCATGA 2 cut(s) 1429, 2310
BspLI GGNNCC 7 cut(s) 7, 465, 1164, 2380, 2820, 2853, 3440
BspOI GCTAGC 1 cut(s) 248
BspQI GCTCTTC 2 cut(s) 562, 1299
BspT107I GGYRCC 1 cut(s) 2378
BspTI CTTAAG 1 cut(s) 3113
BspTNI GGTCTC 1 cut(s) 3001
BsrBI CCGCTC 1 cut(s) 3413
BsrDI GCAATG 4 cut(s) 89, 1349, 1623, 2224
BsrFI RCCGGY 2 cut(s) 159, 2815
BsrGI TGTACA 1 cut(s) 1929
BsrI ACTGG 4 cut(s) 446, 672, 1458, 3280
BssAI RCCGGY 2 cut(s) 159, 2815
BssECI CCNNGG 6 cut(s) 56, 237, 357, 996, 1651, 2129
BssNAI GTATAC 1 cut(s) 958
BssNI GRCGYC 3 cut(s) 687, 727, 3075
BssT1I CCWWGG 2 cut(s) 237, 357
Bst1107I GTATAC 1 cut(s) 958
Bst2UI CCWGG 3 cut(s) 197, 1089, 2066
Bst4CI ACNGT 4 cut(s) 1274, 3017, 3254, 3472
Bst6I CTCTTC 4 cut(s) 20, 562, 645, 1299
BstACI GRCGYC 3 cut(s) 687, 727, 3075
BstAFI CTTAAG 1 cut(s) 3113
BstAUI TGTACA 1 cut(s) 1929
BstBAI YACGTR 1 cut(s) 3353
BstC8I GCNNGC 6 cut(s) 246, 908, 1209, 1750, 2926, 3073
BstDSI CCRYGG 1 cut(s) 996
BstFNI CGCG 2 cut(s) 205, 815
BstH2I RGCGCY 1 cut(s) 3061
BstHHI GCGC 2 cut(s) 207, 3060
BstMAI GTCTC 4 cut(s) 261, 718, 3001, 3095
BstMWI GCNNNNNNNGC 3 cut(s) 821, 1078, 2728
BstNI CCWGG 3 cut(s) 197, 1089, 2066
BstNSI RCATGY 7 cut(s) 437, 739, 1211, 1262, 2083, 2105, 2845
BstSCI CCNGG 4 cut(s) 195, 1087, 1651, 2064
BstSFI CTRYAG 2 cut(s) 229, 2415
BstSLI GKGCMC 2 cut(s) 78, 2593
BstUI CGCG 2 cut(s) 205, 815
BstV1I GCAGC 8 cut(s) 689, 870, 1772, 2285, 2552, 2589, 2964, 3307
BstV2I GAAGAC 1 cut(s) 343
BstX2I RGATCY 6 cut(s) 463, 1307, 2330, 2341, 2623, 3438
BstYI RGATCY 6 cut(s) 463, 1307, 2330, 2341, 2623, 3438
BstZ17I GTATAC 1 cut(s) 958
Bsu15I ATCGAT 5 cut(s) 341, 711, 987, 2982, 3504
Bsu36I CCTNAGG 2 cut(s) 1911, 2168
BsuI GTATCC 1 cut(s) 2255
BsuRI GGCC 8 cut(s) 61, 195, 821, 1576, 1731, 2166, 2819, 3111
BsuTUI ATCGAT 5 cut(s) 341, 711, 987, 2982, 3504
BtgI CCRYGG 1 cut(s) 996
BtrI CACGTC 1 cut(s) 1366
BtsIMutI CAGTG 1 cut(s) 1270
BtuMI TCGCGA 1 cut(s) 815
Cac8I GCNNGC 6 cut(s) 246, 908, 1209, 1750, 2926, 3073
CaiI CAGNNNCTG 2 cut(s) 41, 1883
CciI TCATGA 2 cut(s) 1429, 2310
CfoI GCGC 2 cut(s) 207, 3060
Cfr10I RCCGGY 2 cut(s) 159, 2815
Cfr13I GGNCC 4 cut(s) 1729, 2818, 2851, 3109
ClaI ATCGAT 5 cut(s) 341, 711, 987, 2982, 3504
CseI GACGC 3 cut(s) 576, 735, 3064
CsiI ACCWGGT 1 cut(s) 2064
CspAI ACCGGT 1 cut(s) 159
CspCI CAANNNNNGTGG 4 cut(s) 1242, 1277, 2724, 2759
DraI TTTAAA 1 cut(s) 3235
DrdI GACNNNNNNGTC 1 cut(s) 3257
DseDI GACNNNNNNGTC 1 cut(s) 3257
EaeI YGGCCR 1 cut(s) 1574
Eam1104I CTCTTC 4 cut(s) 20, 562, 645, 1299
EarI CTCTTC 4 cut(s) 20, 562, 645, 1299
Ecl136II GAGCTC 2 cut(s) 572, 2731
Eco130I CCWWGG 2 cut(s) 237, 357
Eco24I GRGCYC 2 cut(s) 574, 2733
Eco31I GGTCTC 1 cut(s) 3001
Eco32I GATATC 2 cut(s) 931, 2980
Eco47I GGWCC 1 cut(s) 2851
Eco53kI GAGCTC 2 cut(s) 572, 2731
Eco57I CTGAAG 2 cut(s) 870, 1198
Eco72I CACGTG 1 cut(s) 3353
Eco81I CCTNAGG 2 cut(s) 1911, 2168
EcoICRI GAGCTC 2 cut(s) 572, 2731
EcoO109I RGGNCCY 2 cut(s) 1729, 3109
EcoRII CCWGG 3 cut(s) 195, 1087, 2064
EcoRV GATATC 2 cut(s) 931, 2980
EcoT14I CCWWGG 2 cut(s) 237, 357
EcoT22I ATGCAT 2 cut(s) 1596, 3343
EcoT38I GRGCYC 2 cut(s) 574, 2733
ErhI CCWWGG 2 cut(s) 237, 357
Esp3I CGTCTC 2 cut(s) 261, 718
FaqI GGGAC 2 cut(s) 13, 3305
FauI CCCGC 3 cut(s) 2331, 3209, 3530
FbaI TGATCA 3 cut(s) 220, 1426, 2401
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 9 cut(s) 678, 822, 859, 1786, 2299, 2566, 2603, 2953, 3296
FriOI GRGCYC 2 cut(s) 574, 2733
Fsp4HI GCNGC 9 cut(s) 678, 822, 859, 1786, 2299, 2566, 2603, 2953, 3296
FspBI CTAG 6 cut(s) 245, 1544, 1559, 1665, 2267, 2912
GlaI GCGC 2 cut(s) 206, 3059
GluI GCNGC 9 cut(s) 678, 822, 859, 1786, 2299, 2566, 2603, 2953, 3296
HaeII RGCGCY 1 cut(s) 3061
HaeIII GGCC 8 cut(s) 61, 195, 821, 1576, 1731, 2166, 2819, 3111
HapII CCGG 4 cut(s) 160, 1320, 1653, 2816
HgaI GACGC 3 cut(s) 576, 735, 3064
HhaI GCGC 2 cut(s) 207, 3060
Hin1I GRCGYC 3 cut(s) 687, 727, 3075
Hin6I GCGC 2 cut(s) 205, 3058
HinP1I GCGC 2 cut(s) 205, 3058
HincII GTYRAC 3 cut(s) 1549, 1963, 3468
HindII GTYRAC 3 cut(s) 1549, 1963, 3468
HindIII AAGCTT 4 cut(s) 2315, 2366, 2678, 2926
HpaII CCGG 4 cut(s) 160, 1320, 1653, 2816
HphI GGTGA 5 cut(s) 215, 1615, 1632, 2442, 2805
Hpy166II GTNNAC 8 cut(s) 958, 1549, 1963, 2531, 2552, 3135, 3289, 3468
Hpy8I GTNNAC 8 cut(s) 958, 1549, 1963, 2531, 2552, 3135, 3289, 3468
Hpy99I CGWCG 2 cut(s) 692, 2482
HpyCH4III ACNGT 4 cut(s) 1274, 3017, 3254, 3472
HpyF10VI GCNNNNNNNGC 3 cut(s) 821, 1078, 2728
Hsp92I GRCGYC 3 cut(s) 687, 727, 3075
HspAI GCGC 2 cut(s) 205, 3058
Kpn2I TCCGGA 1 cut(s) 1319
Ksp22I TGATCA 3 cut(s) 220, 1426, 2401
LguI GCTCTTC 2 cut(s) 562, 1299
LmnI GCTCC 7 cut(s) 241, 556, 697, 1246, 2736, 3065, 3410
Lsp1109I GCAGC 8 cut(s) 689, 870, 1772, 2285, 2552, 2589, 2964, 3307
LweI GCATC 6 cut(s) 118, 1544, 1603, 1672, 1895, 3328
MabI ACCWGGT 1 cut(s) 2064
MaeI CTAG 6 cut(s) 245, 1544, 1559, 1665, 2267, 2912
MaeIII GTNAC 7 cut(s) 330, 741, 1238, 2867, 3259, 3443, 3488
MbiI CCGCTC 1 cut(s) 3413
MfeI CAATTG 2 cut(s) 1598, 2750
MflI RGATCY 6 cut(s) 463, 1307, 2330, 2341, 2623, 3438
MhlI GDGCHC 5 cut(s) 78, 574, 809, 2593, 2733
MlyI GAGTC 7 cut(s) 311, 338, 2187, 2261, 2879, 3285, 3512
MmeI TCCRAC 5 cut(s) 285, 388, 685, 1482, 1747
Mph1103I ATGCAT 2 cut(s) 1596, 3343
MroI TCCGGA 1 cut(s) 1319
MroXI GAANNNNTTC 3 cut(s) 527, 1626, 1986
MslI CAYNNNNRTG 4 cut(s) 23, 71, 1616, 2160
MspCI CTTAAG 1 cut(s) 3113
MspI CCGG 4 cut(s) 160, 1320, 1653, 2816
MspR9I CCNGG 4 cut(s) 197, 1089, 1653, 2066
MunI CAATTG 2 cut(s) 1598, 2750
Mva1269I GAATGC 5 cut(s) 81, 1074, 1138, 2536, 3218
MvaI CCWGG 3 cut(s) 197, 1089, 2066
MvnI CGCG 2 cut(s) 205, 815
MwoI GCNNNNNNNGC 3 cut(s) 821, 1078, 2728
NciI CCSGG 1 cut(s) 1653
NheI GCTAGC 1 cut(s) 244
NlaIV GGNNCC 7 cut(s) 7, 465, 1164, 2380, 2820, 2853, 3440
NmeAIII GCCGAG 2 cut(s) 37, 2514
NmuCI GTSAC 2 cut(s) 330, 3259
NruI TCGCGA 1 cut(s) 815
NsiI ATGCAT 2 cut(s) 1596, 3343
NspI RCATGY 7 cut(s) 437, 739, 1211, 1262, 2083, 2105, 2845
PaeI GCATGC 1 cut(s) 1211
PagI TCATGA 2 cut(s) 1429, 2310
PciI ACATGT 5 cut(s) 433, 735, 1258, 2101, 2841
PciSI GCTCTTC 2 cut(s) 562, 1299
PcsI WCGNNNNNNNCGW 4 cut(s) 993, 2486, 2988, 2997
PctI GAATGC 5 cut(s) 81, 1074, 1138, 2536, 3218
PdmI GAANNNNTTC 3 cut(s) 527, 1626, 1986
PfoI TCCNGGA 1 cut(s) 1087
PinAI ACCGGT 1 cut(s) 159
PkrI GCNGC 9 cut(s) 679, 823, 860, 1787, 2300, 2567, 2604, 2954, 3297
PleI GAGTC 7 cut(s) 310, 337, 2187, 2260, 2879, 3284, 3512
PmaCI CACGTG 1 cut(s) 3353
PmlI CACGTG 1 cut(s) 3353
PpsI GAGTC 7 cut(s) 310, 337, 2187, 2260, 2879, 3284, 3512
Ppu21I YACGTR 1 cut(s) 3353
PscI ACATGT 5 cut(s) 433, 735, 1258, 2101, 2841
PsiI TTATAA 1 cut(s) 828
Psp124BI GAGCTC 2 cut(s) 574, 2733
Psp1406I AACGTT 1 cut(s) 1760
Psp6I CCWGG 3 cut(s) 195, 1087, 2064
PspCI CACGTG 1 cut(s) 3353
PspGI CCWGG 3 cut(s) 195, 1087, 2064
PspN4I GGNNCC 7 cut(s) 7, 465, 1164, 2380, 2820, 2853, 3440
PspPI GGNCC 4 cut(s) 1729, 2818, 2851, 3109
PstNI CAGNNNCTG 2 cut(s) 41, 1883
PsuI RGATCY 6 cut(s) 463, 1307, 2330, 2341, 2623, 3438
RruI TCGCGA 1 cut(s) 815
RseI CAYNNNNRTG 4 cut(s) 23, 71, 1616, 2160
SacI GAGCTC 2 cut(s) 574, 2733
SapI GCTCTTC 2 cut(s) 562, 1299
SatI GCNGC 9 cut(s) 678, 822, 859, 1786, 2299, 2566, 2603, 2953, 3296
Sau96I GGNCC 4 cut(s) 1729, 2818, 2851, 3109
ScaI AGTACT 1 cut(s) 2618
SchI GAGTC 7 cut(s) 311, 338, 2187, 2261, 2879, 3285, 3512
ScrFI CCNGG 4 cut(s) 197, 1089, 1653, 2066
SduI GDGCHC 5 cut(s) 78, 574, 809, 2593, 2733
SexAI ACCWGGT 1 cut(s) 2064
SfaNI GCATC 6 cut(s) 118, 1544, 1603, 1672, 1895, 3328
SfcI CTRYAG 2 cut(s) 229, 2415
SinI GGWCC 1 cut(s) 2851
SmiMI CAYNNNNRTG 4 cut(s) 23, 71, 1616, 2160
SmlI CTYRAG 5 cut(s) 181, 373, 1190, 2204, 3113
SmoI CTYRAG 5 cut(s) 181, 373, 1190, 2204, 3113
SpeI ACTAGT 1 cut(s) 2911
SphI GCATGC 1 cut(s) 1211
SsiI CCGC 7 cut(s) 822, 832, 2338, 2534, 3216, 3413, 3523
SspI AATATT 3 cut(s) 1693, 2325, 2500
SspMI CTAG 6 cut(s) 245, 1544, 1559, 1665, 2267, 2912
SstI GAGCTC 2 cut(s) 574, 2733
StyD4I CCNGG 4 cut(s) 195, 1087, 1651, 2064
StyI CCWWGG 2 cut(s) 237, 357
TaaI ACNGT 4 cut(s) 1274, 3017, 3254, 3472
TaqI TCGA 9 cut(s) 341, 711, 987, 1114, 2982, 2991, 3000, 3030, 3504
TatI WGTACW 3 cut(s) 1493, 1929, 2616
TauI GCSGC 1 cut(s) 824
TscAI CASTG 1 cut(s) 1277
TseFI GTSAC 2 cut(s) 330, 3259
TseI GCWGC 8 cut(s) 677, 858, 1785, 2298, 2565, 2602, 2952, 3295
Tsp45I GTSAC 2 cut(s) 330, 3259
TspGWI ACGGA 4 cut(s) 551, 980, 1730, 3431
TspRI CASTG 1 cut(s) 1277
Vha464I CTTAAG 1 cut(s) 3113
VpaK11BI GGWCC 1 cut(s) 2851
XceI RCATGY 7 cut(s) 437, 739, 1211, 1262, 2083, 2105, 2845
XmiI GTMKAC 1 cut(s) 957
XmnI GAANNNNTTC 3 cut(s) 527, 1626, 1986
XspI CTAG 6 cut(s) 245, 1544, 1559, 1665, 2267, 2912
ZraI GACGTC 1 cut(s) 688
ZrmI AGTACT 1 cut(s) 2618
Zsp2I ATGCAT 2 cut(s) 1596, 3343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.