Cyclic phosphodiesterase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 377, 639, 1161 |
| AccBSI | CCGCTC | 1 cut(s) | 1207 |
| AccI | GTMKAC | 1 cut(s) | 660 |
| AccIII | TCCGGA | 2 cut(s) | 1350, 1618 |
| AciI | CCGC | 4 cut(s) | 775, 1207, 1378, 1392 |
| AclWI | GGATC | 2 cut(s) | 551, 1400 |
| AcoI | YGGCCR | 1 cut(s) | 506 |
| AcsI | RAATTY | 4 cut(s) | 326, 899, 1019, 1385 |
| AcuI | CTGAAG | 1 cut(s) | 98 |
| AfaI | GTAC | 4 cut(s) | 673, 697, 1498, 1670 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 129, 525, 1037, 1425 |
| AgeI | ACCGGT | 1 cut(s) | 347 |
| AgsI | TTSAA | 1 cut(s) | 1117 |
| AhlI | ACTAGT | 1 cut(s) | 179 |
| AjnI | CCWGG | 2 cut(s) | 128, 321 |
| Alw21I | GWGCWC | 1 cut(s) | 1071 |
| Alw26I | GTCTC | 4 cut(s) | 100, 221, 1350, 1700 |
| AlwI | GGATC | 2 cut(s) | 551, 1400 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 425 |
| Aor13HI | TCCGGA | 2 cut(s) | 1350, 1618 |
| AoxI | GGCC | 5 cut(s) | 385, 506, 731, 1208, 1394 |
| ApeKI | GCWGC | 5 cut(s) | 419, 653, 959, 1239, 1635 |
| ApoI | RAATTY | 4 cut(s) | 326, 899, 1019, 1385 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 642, 674 |
| AsiGI | ACCGGT | 1 cut(s) | 347 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 83 |
| AspS9I | GGNCC | 5 cut(s) | 732, 1028, 1121, 1394, 1428 |
| AsuHPI | GGTGA | 4 cut(s) | 112, 440, 445, 1021 |
| AsuII | TTCGAA | 2 cut(s) | 259, 1198 |
| AvaII | GGWCC | 3 cut(s) | 1028, 1121, 1428 |
| BalI | TGGCCA | 1 cut(s) | 508 |
| BanII | GRGCYC | 1 cut(s) | 1071 |
| BbsI | GAAGAC | 1 cut(s) | 1632 |
| Bbv12I | GWGCWC | 1 cut(s) | 1071 |
| BbvCI | CCTCAGC | 1 cut(s) | 1631 |
| BbvI | GCAGC | 5 cut(s) | 431, 665, 946, 1251, 1647 |
| BccI | CCATC | 7 cut(s) | 278, 530, 617, 919, 997, 1339, 1360 |
| BceAI | ACGGC | 2 cut(s) | 641, 1403 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 72, 106, 1314, 1348 |
| BciT130I | CCWGG | 2 cut(s) | 130, 323 |
| BciVI | GTATCC | 1 cut(s) | 329 |
| BcoDI | GTCTC | 4 cut(s) | 100, 221, 1350, 1700 |
| BcuI | ACTAGT | 1 cut(s) | 179 |
| BfaI | CTAG | 4 cut(s) | 180, 315, 410, 1526 |
| BfuI | GTATCC | 1 cut(s) | 329 |
| BglII | AGATCT | 1 cut(s) | 1469 |
| BisI | GCNGC | 6 cut(s) | 420, 654, 960, 1208, 1240, 1636 |
| BlpI | GCTNAGC | 1 cut(s) | 141 |
| BlsI | GCNGC | 6 cut(s) | 421, 655, 961, 1209, 1241, 1637 |
| BmcAI | AGTACT | 1 cut(s) | 697 |
| Bme1390I | CCNGG | 2 cut(s) | 130, 323 |
| Bme18I | GGWCC | 3 cut(s) | 1028, 1121, 1428 |
| BmgT120I | GGNCC | 5 cut(s) | 732, 1028, 1121, 1394, 1428 |
| BmiI | GGNNCC | 3 cut(s) | 825, 1080, 1429 |
| BmrFI | CCNGG | 2 cut(s) | 130, 323 |
| BmrI | ACTGGG | 1 cut(s) | 870 |
| BmsI | GCATC | 2 cut(s) | 738, 1272 |
| BmuI | ACTGGG | 1 cut(s) | 870 |
| BpiI | GAAGAC | 1 cut(s) | 1632 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 227, 259 |
| BpmI | CTGGAG | 2 cut(s) | 588, 1460 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1631 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 141 |
| Bpu14I | TTCGAA | 2 cut(s) | 259, 1198 |
| BpuEI | CTTGAG | 5 cut(s) | 216, 345, 399, 1584, 1660 |
| BsaJI | CCNNGG | 2 cut(s) | 128, 667 |
| BsaWI | WCCGGW | 5 cut(s) | 347, 525, 892, 1350, 1618 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 158, 188 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 129, 525, 1037, 1425 |
| Bse118I | RCCGGY | 1 cut(s) | 347 |
| Bse1I | ACTGG | 3 cut(s) | 88, 865, 1477 |
| Bse3DI | GCAATG | 1 cut(s) | 1413 |
| BseAI | TCCGGA | 2 cut(s) | 1350, 1618 |
| BseBI | CCWGG | 2 cut(s) | 130, 323 |
| BseDI | CCNNGG | 2 cut(s) | 128, 667 |
| BseGI | GGATG | 5 cut(s) | 808, 871, 901, 1342, 1627 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 129, 525, 1037, 1425 |
| BseMI | GCAATG | 1 cut(s) | 1413 |
| BseMII | CTCAG | 1 cut(s) | 1645 |
| BseNI | ACTGG | 3 cut(s) | 88, 865, 1477 |
| BseRI | GAGGAG | 1 cut(s) | 836 |
| BseXI | GCAGC | 5 cut(s) | 431, 665, 946, 1251, 1647 |
| BseYI | CCCAGC | 1 cut(s) | 448 |
| BshFI | GGCC | 5 cut(s) | 387, 508, 733, 1210, 1396 |
| BshTI | ACCGGT | 1 cut(s) | 347 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1071 |
| BsiSI | CCGG | 6 cut(s) | 348, 526, 893, 1351, 1397, 1619 |
| BslFI | GGGAC | 2 cut(s) | 792, 1386 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 129, 525, 1037, 1425 |
| BsmAI | GTCTC | 4 cut(s) | 100, 221, 1350, 1700 |
| BsmBI | CGTCTC | 1 cut(s) | 100 |
| BsmFI | GGGAC | 2 cut(s) | 792, 1386 |
| BsnI | GGCC | 5 cut(s) | 387, 508, 733, 1210, 1396 |
| Bsp119I | TTCGAA | 2 cut(s) | 259, 1198 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1071 |
| Bsp13I | TCCGGA | 2 cut(s) | 1350, 1618 |
| Bsp1407I | TGTACA | 1 cut(s) | 1668 |
| Bsp143I | GATC | 4 cut(s) | 556, 690, 1405, 1469 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 141 |
| BspACI | CCGC | 4 cut(s) | 775, 1207, 1378, 1392 |
| BspANI | GGCC | 5 cut(s) | 387, 508, 733, 1210, 1396 |
| BspCNI | CTCAG | 1 cut(s) | 1644 |
| BspEI | TCCGGA | 2 cut(s) | 1350, 1618 |
| BspHI | TCATGA | 1 cut(s) | 457 |
| BspLI | GGNNCC | 3 cut(s) | 825, 1080, 1429 |
| BspPI | GGATC | 2 cut(s) | 551, 1400 |
| BspQI | GCTCTTC | 1 cut(s) | 1091 |
| BspT104I | TTCGAA | 2 cut(s) | 259, 1198 |
| BsrBI | CCGCTC | 1 cut(s) | 1207 |
| BsrDI | GCAATG | 1 cut(s) | 1413 |
| BsrFI | RCCGGY | 1 cut(s) | 347 |
| BsrGI | TGTACA | 1 cut(s) | 1668 |
| BsrI | ACTGG | 3 cut(s) | 88, 865, 1477 |
| BssAI | RCCGGY | 1 cut(s) | 347 |
| BssECI | CCNNGG | 2 cut(s) | 128, 667 |
| BssMI | GATC | 4 cut(s) | 556, 690, 1405, 1469 |
| BssT1I | CCWWGG | 1 cut(s) | 667 |
| Bst2UI | CCWGG | 2 cut(s) | 130, 323 |
| Bst4CI | ACNGT | 1 cut(s) | 1083 |
| Bst6I | CTCTTC | 2 cut(s) | 1091, 1578 |
| BstAUI | TGTACA | 1 cut(s) | 1668 |
| BstBI | TTCGAA | 2 cut(s) | 259, 1198 |
| BstC8I | GCNNGC | 2 cut(s) | 1205, 1394 |
| BstDEI | CTNAG | 3 cut(s) | 141, 940, 1631 |
| BstEII | GGTNACC | 1 cut(s) | 1569 |
| BstF5I | GGATG | 5 cut(s) | 808, 871, 901, 1342, 1627 |
| BstKTI | GATC | 4 cut(s) | 559, 693, 1408, 1472 |
| BstMAI | GTCTC | 4 cut(s) | 100, 221, 1350, 1700 |
| BstMBI | GATC | 4 cut(s) | 556, 690, 1405, 1469 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 298, 419, 1641 |
| BstNI | CCWGG | 2 cut(s) | 130, 323 |
| BstPI | GGTNACC | 1 cut(s) | 1569 |
| BstSCI | CCNGG | 2 cut(s) | 128, 321 |
| BstV1I | GCAGC | 5 cut(s) | 431, 665, 946, 1251, 1647 |
| BstV2I | GAAGAC | 1 cut(s) | 1632 |
| BstX2I | RGATCY | 1 cut(s) | 1469 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 395 |
| BstYI | RGATCY | 1 cut(s) | 1469 |
| BsuI | GTATCC | 1 cut(s) | 329 |
| BsuRI | GGCC | 5 cut(s) | 387, 508, 733, 1210, 1396 |
| BtgZI | GCGATG | 1 cut(s) | 1116 |
| BtsCI | GGATG | 5 cut(s) | 808, 871, 901, 1342, 1627 |
| BtsIMutI | CAGTG | 2 cut(s) | 95, 1131 |
| Cac8I | GCNNGC | 2 cut(s) | 1205, 1394 |
| CaiI | CAGNNNCTG | 1 cut(s) | 425 |
| CciI | TCATGA | 1 cut(s) | 457 |
| Cfr10I | RCCGGY | 1 cut(s) | 347 |
| Cfr13I | GGNCC | 5 cut(s) | 732, 1028, 1121, 1394, 1428 |
| CseI | GACGC | 1 cut(s) | 82 |
| Csp6I | GTAC | 4 cut(s) | 672, 696, 1497, 1669 |
| CspAI | ACCGGT | 1 cut(s) | 347 |
| CviAII | CATG | 7 cut(s) | 160, 293, 458, 510, 782, 1445, 1704 |
| CviQI | GTAC | 4 cut(s) | 672, 696, 1497, 1669 |
| DdeI | CTNAG | 3 cut(s) | 141, 940, 1631 |
| DpnI | GATC | 4 cut(s) | 558, 692, 1407, 1471 |
| DpnII | GATC | 4 cut(s) | 556, 690, 1405, 1469 |
| EaeI | YGGCCR | 1 cut(s) | 506 |
| Eam1104I | CTCTTC | 2 cut(s) | 1091, 1578 |
| EarI | CTCTTC | 2 cut(s) | 1091, 1578 |
| Ecl136II | GAGCTC | 1 cut(s) | 1069 |
| Eco130I | CCWWGG | 1 cut(s) | 667 |
| Eco24I | GRGCYC | 1 cut(s) | 1071 |
| Eco47I | GGWCC | 3 cut(s) | 1028, 1121, 1428 |
| Eco53kI | GAGCTC | 1 cut(s) | 1069 |
| Eco57I | CTGAAG | 1 cut(s) | 98 |
| Eco91I | GGTNACC | 1 cut(s) | 1569 |
| EcoICRI | GAGCTC | 1 cut(s) | 1069 |
| EcoO65I | GGTNACC | 1 cut(s) | 1569 |
| EcoRI | GAATTC | 2 cut(s) | 326, 1385 |
| EcoRII | CCWGG | 2 cut(s) | 128, 321 |
| EcoT14I | CCWWGG | 1 cut(s) | 667 |
| EcoT38I | GRGCYC | 1 cut(s) | 1071 |
| ErhI | CCWWGG | 1 cut(s) | 667 |
| Esp3I | CGTCTC | 1 cut(s) | 100 |
| FaeI | CATG | 7 cut(s) | 163, 296, 461, 513, 785, 1448, 1707 |
| FaqI | GGGAC | 2 cut(s) | 792, 1386 |
| FatI | CATG | 7 cut(s) | 159, 292, 457, 509, 781, 1444, 1703 |
| FauI | CCCGC | 2 cut(s) | 768, 1385 |
| FauNDI | CATATG | 1 cut(s) | 997 |
| FblI | GTMKAC | 1 cut(s) | 660 |
| Fnu4HI | GCNGC | 6 cut(s) | 420, 654, 960, 1208, 1240, 1636 |
| FokI | GGATG | 5 cut(s) | 815, 878, 908, 1349, 1634 |
| FriOI | GRGCYC | 1 cut(s) | 1071 |
| Fsp4HI | GCNGC | 6 cut(s) | 420, 654, 960, 1208, 1240, 1636 |
| FspBI | CTAG | 4 cut(s) | 180, 315, 410, 1526 |
| GluI | GCNGC | 6 cut(s) | 420, 654, 960, 1208, 1240, 1636 |
| GsaI | CCCAGC | 1 cut(s) | 452 |
| GsuI | CTGGAG | 2 cut(s) | 588, 1460 |
| HaeIII | GGCC | 5 cut(s) | 387, 508, 733, 1210, 1396 |
| HapII | CCGG | 6 cut(s) | 348, 526, 893, 1351, 1397, 1619 |
| HgaI | GACGC | 1 cut(s) | 82 |
| Hin1II | CATG | 7 cut(s) | 163, 296, 461, 513, 785, 1448, 1707 |
| HincII | GTYRAC | 1 cut(s) | 986 |
| HindII | GTYRAC | 1 cut(s) | 986 |
| HindIII | AAGCTT | 2 cut(s) | 467, 1178 |
| HpaII | CCGG | 6 cut(s) | 348, 526, 893, 1351, 1397, 1619 |
| HphI | GGTGA | 4 cut(s) | 112, 440, 445, 1021 |
| Hpy166II | GTNNAC | 2 cut(s) | 661, 986 |
| Hpy188I | TCNGA | 8 cut(s) | 78, 223, 502, 533, 561, 933, 1336, 1469 |
| Hpy188III | TCNNGA | 8 cut(s) | 233, 458, 494, 605, 745, 1351, 1601, 1619 |
| Hpy8I | GTNNAC | 2 cut(s) | 661, 986 |
| Hpy99I | CGWCG | 2 cut(s) | 821, 1580 |
| HpyAV | CCTTC | 2 cut(s) | 500, 943 |
| HpyCH4III | ACNGT | 1 cut(s) | 1083 |
| HpyCH4IV | ACGT | 2 cut(s) | 5, 196 |
| HpyCH4V | TGCA | 5 cut(s) | 163, 292, 444, 751, 1664 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 298, 419, 1641 |
| HpyF3I | CTNAG | 3 cut(s) | 141, 940, 1631 |
| HpySE526I | ACGT | 2 cut(s) | 5, 196 |
| Hsp92II | CATG | 7 cut(s) | 163, 296, 461, 513, 785, 1448, 1707 |
| Kpn2I | TCCGGA | 2 cut(s) | 1350, 1618 |
| Kzo9I | GATC | 4 cut(s) | 556, 690, 1405, 1469 |
| LguI | GCTCTTC | 1 cut(s) | 1091 |
| LmnI | GCTCC | 3 cut(s) | 607, 823, 1074 |
| Lsp1109I | GCAGC | 5 cut(s) | 431, 665, 946, 1251, 1647 |
| LweI | GCATC | 2 cut(s) | 738, 1272 |
| MaeI | CTAG | 4 cut(s) | 180, 315, 410, 1526 |
| MaeII | ACGT | 2 cut(s) | 5, 196 |
| MaeIII | GTNAC | 3 cut(s) | 888, 1521, 1569 |
| MalI | GATC | 4 cut(s) | 558, 692, 1407, 1471 |
| MbiI | CCGCTC | 1 cut(s) | 1207 |
| MboI | GATC | 4 cut(s) | 556, 690, 1405, 1469 |
| MflI | RGATCY | 1 cut(s) | 1469 |
| MhlI | GDGCHC | 1 cut(s) | 1071 |
| MlsI | TGGCCA | 1 cut(s) | 508 |
| MluNI | TGGCCA | 1 cut(s) | 508 |
| MlyI | GAGTC | 5 cut(s) | 210, 223, 352, 947, 1208 |
| MmeI | TCCRAC | 1 cut(s) | 662 |
| Mox20I | TGGCCA | 1 cut(s) | 508 |
| MroI | TCCGGA | 2 cut(s) | 1350, 1618 |
| MroXI | GAANNNNTTC | 1 cut(s) | 83 |
| MscI | TGGCCA | 1 cut(s) | 508 |
| MseI | TTAA | 9 cut(s) | 393, 567, 585, 630, 756, 1182, 1356, 1511, 1677 |
| MslI | CAYNNNNRTG | 2 cut(s) | 393, 1436 |
| Msp20I | TGGCCA | 1 cut(s) | 508 |
| MspI | CCGG | 6 cut(s) | 348, 526, 893, 1351, 1397, 1619 |
| MspR9I | CCNGG | 2 cut(s) | 130, 323 |
| MvaI | CCWGG | 2 cut(s) | 130, 323 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 298, 419, 1641 |
| NdeI | CATATG | 1 cut(s) | 997 |
| NdeII | GATC | 4 cut(s) | 556, 690, 1405, 1469 |
| NlaIII | CATG | 7 cut(s) | 163, 296, 461, 513, 785, 1448, 1707 |
| NlaIV | GGNNCC | 3 cut(s) | 825, 1080, 1429 |
| NspV | TTCGAA | 2 cut(s) | 259, 1198 |
| PagI | TCATGA | 1 cut(s) | 457 |
| PciSI | GCTCTTC | 1 cut(s) | 1091 |
| PdmI | GAANNNNTTC | 1 cut(s) | 83 |
| PfeI | GAWTC | 5 cut(s) | 84, 368, 399, 497, 701 |
| PfoI | TCCNGGA | 1 cut(s) | 321 |
| PinAI | ACCGGT | 1 cut(s) | 347 |
| PkrI | GCNGC | 6 cut(s) | 421, 655, 961, 1209, 1241, 1637 |
| PleI | GAGTC | 5 cut(s) | 209, 223, 352, 947, 1208 |
| PpsI | GAGTC | 5 cut(s) | 209, 223, 352, 947, 1208 |
| PsiI | TTATAA | 3 cut(s) | 377, 639, 1161 |
| Psp124BI | GAGCTC | 1 cut(s) | 1071 |
| Psp6I | CCWGG | 2 cut(s) | 128, 321 |
| PspEI | GGTNACC | 1 cut(s) | 1569 |
| PspFI | CCCAGC | 1 cut(s) | 448 |
| PspGI | CCWGG | 2 cut(s) | 128, 321 |
| PspN4I | GGNNCC | 3 cut(s) | 825, 1080, 1429 |
| PspPI | GGNCC | 5 cut(s) | 732, 1028, 1121, 1394, 1428 |
| PstNI | CAGNNNCTG | 1 cut(s) | 425 |
| PsuI | RGATCY | 1 cut(s) | 1469 |
| RsaI | GTAC | 4 cut(s) | 673, 697, 1498, 1670 |
| RsaNI | GTAC | 4 cut(s) | 672, 696, 1497, 1669 |
| RseI | CAYNNNNRTG | 2 cut(s) | 393, 1436 |
| SacI | GAGCTC | 1 cut(s) | 1071 |
| SapI | GCTCTTC | 1 cut(s) | 1091 |
| SaqAI | TTAA | 9 cut(s) | 393, 567, 585, 630, 756, 1182, 1356, 1511, 1677 |
| SatI | GCNGC | 6 cut(s) | 420, 654, 960, 1208, 1240, 1636 |
| Sau3AI | GATC | 4 cut(s) | 556, 690, 1405, 1469 |
| Sau96I | GGNCC | 5 cut(s) | 732, 1028, 1121, 1394, 1428 |
| ScaI | AGTACT | 1 cut(s) | 697 |
| SchI | GAGTC | 5 cut(s) | 210, 223, 352, 947, 1208 |
| ScrFI | CCNGG | 2 cut(s) | 130, 323 |
| SduI | GDGCHC | 1 cut(s) | 1071 |
| SfaNI | GCATC | 2 cut(s) | 738, 1272 |
| SfuI | TTCGAA | 2 cut(s) | 259, 1198 |
| SgrAI | CRCCGGYG | 1 cut(s) | 347 |
| SinI | GGWCC | 3 cut(s) | 1028, 1121, 1428 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 393, 1436 |
| SmlI | CTYRAG | 5 cut(s) | 231, 360, 414, 1599, 1639 |
| SmoI | CTYRAG | 5 cut(s) | 231, 360, 414, 1599, 1639 |
| SpeI | ACTAGT | 1 cut(s) | 179 |
| SsiI | CCGC | 4 cut(s) | 775, 1207, 1378, 1392 |
| SspMI | CTAG | 4 cut(s) | 180, 315, 410, 1526 |
| SstI | GAGCTC | 1 cut(s) | 1071 |
| StyD4I | CCNGG | 2 cut(s) | 128, 321 |
| StyI | CCWWGG | 1 cut(s) | 667 |
| TaaI | ACNGT | 1 cut(s) | 1083 |
| TaiI | ACGT | 2 cut(s) | 8, 199 |
| TaqI | TCGA | 4 cut(s) | 259, 1198, 1481, 1657 |
| TatI | WGTACW | 2 cut(s) | 695, 1668 |
| TauI | GCSGC | 1 cut(s) | 1210 |
| TfiI | GAWTC | 5 cut(s) | 84, 368, 399, 497, 701 |
| Tru1I | TTAA | 9 cut(s) | 393, 567, 585, 630, 756, 1182, 1356, 1511, 1677 |
| Tru9I | TTAA | 9 cut(s) | 393, 567, 585, 630, 756, 1182, 1356, 1511, 1677 |
| TscAI | CASTG | 2 cut(s) | 95, 1138 |
| TseI | GCWGC | 5 cut(s) | 419, 653, 959, 1239, 1635 |
| TspGWI | ACGGA | 1 cut(s) | 327 |
| TspRI | CASTG | 2 cut(s) | 95, 1138 |
| VpaK11BI | GGWCC | 3 cut(s) | 1028, 1121, 1428 |
| XapI | RAATTY | 4 cut(s) | 326, 899, 1019, 1385 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 501 |
| XmiI | GTMKAC | 1 cut(s) | 660 |
| XmnI | GAANNNNTTC | 1 cut(s) | 83 |
| XspI | CTAG | 4 cut(s) | 180, 315, 410, 1526 |
| ZrmI | AGTACT | 1 cut(s) | 697 |