AT5G33300

Tesmin/TSO1-like CXC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000316
Physical Location & Seq
Reverse (-)
12562426 .. 12565780
3355 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G33300.1

Sequence Viewer

Length: 1320 bp
ATGACGAAGAAGCCAAATCTCCGATCAAAGGATTCATCTCTCGGAGACGATGCTGAGAAACTCTACGAGAAGAAGATTCTGGATCTGGAGTCCGTGAACGAAGCTCTCAAGAGTGATGTGGAAGAGCTAAGGTCAAAATTGGCTGATGTTTCGATAAGCTCATCGGTTGGTACGTTACAGTCAAGCAGAAAGTTTTCTCAAAAGTCTATTGCTAACAAAGAGGAGGGTATGAGTTCAAGGAGTAAGTCTATGTGCTCCACAAAGAAGTATAAAACTGAGAGTTCTGTTAAGCAATTTGATGGTGAAGTTCAGAAATTGAAGGCGCAAAAGGTTAAGTTGCATTGCAAGATCAAGCTAGACTCTATGCAGTTCAGACTATTGAAAGCTTCACTAGAAAAGGAAGTTCTTCAGCTCAAAAAAGAGCTAAGGAAAAGTGAGTTTGAGAAACATGTCCTGTCAGCTCTGAACAATCGCCAGAAGCTGATTTTGCAGCTGAAGAATACACAAGCTTTGACAGCCCTGAAGCGTCTAAAAATGCTGCTTCAATCTAAAAAGATTTCTTCCAACAAAAAGAAAGGCCCATCAAAAGGAACAAGCTCCGAGATTCAGGAGTCTAGCAATGAATCAGGCCTACTAATGAAACTGAATAAAATTCATTCTGATTACGAACGCCAAATGAAAGAAATGGCTGAAGAGATCAAAAGGTTCAGCTTAGAGGCAGGTTTGTTGAAGGCAGAATTTGAGGGAGAGCAAAGCTCATGTTCTGCGTCTTGTGACAACCAAATCAACCACACTCCAATGGACTCAGAACTAAAGGAACTCAAAGAGGAATTTAACAAACTGAGTACTCTTGTCAGCCAGATGGAAATGACCAAATCACAATTCAGTGAAACTGATAAAGTTCAGAGCGAACCAGTGGAACGCTCTATCACCAGCAAGAATATCGACGATGAATCAAACTCGGAGCCATTTCAACCGGAAACTTCTGAGGAAACACTTTGCAAAAAGGAACAGAGCAAAGCAGAAGTGTGTTGCTCTTGCACTAAAAAATCTTTGTGCAAGACCAAAAGTTGCAAATGCAAGGCAAATGGAAGTGGATGTGGGGACTCTTGTGGCTGCTTAGCTTCCAAATGTAGCAACAGAGACGAAAGTGCGAAACCCGACAAACCAATGGAACCAATAGATGTTAAGAAGCCTGCTGGAATTAGCCATGACGACAAAGGCGCAAATAAACAACCATTACGTGACATAGGGAATATACAAGAAGCTGTAAAAGTTGGTAAGTTGAGGAAAGTGCAGAAACGGGTTGCTAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

439

Amino Acids

49.1

Weight (kDa)

9.22

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIF21A_4th PF25764 17 - 141 2.8e-11 KIF21A-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G33300
fragaria_vesca FvH4_3g26472 FvH4_5g30650 FvH4_5g30650 FvH4_5g30650 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250
malus_domestica MD08G1198100.v1.1 MD08G1222000.v1.1 MD15G1385400.v1.1 MD15G1440800.v1.1
prunus_persica Prupe.1G532100_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1
pyrus_communis pycom08g17030 pycom08g17040 pycom08g19270 pycom08g19280 pycom15g34550 pycom15g36860 pycom15g38860
rosa_chinensis RchiOBHm_Chr6g0258571 RchiOBHm_Chr6g0258581 RchiOBHm_Chr6g0258621 RchiOBHm_Chr7g0225511 RchiOBHm_Chr7g0230561 RchiOBHm_Chr7g0230571 RchiOBHm_Chr7g0236371 RchiOBHm_Chr7g0236391
rosa_laevigata RLG00000001080 RLG00000001810 RLG00000014605
rosa_multiflora Rmu_co8399019.1_g000001 Rmu_sc0002222.1_g000016 Rmu_sc0002449.1_g000032 Rmu_sc0003720.1_g000005 Rmu_sc0005578.1_g000003 Rmu_sc0011035.1_g000002 Rmu_sc0012681.1_g000001 Rmu_sc0023362.1_g000001 Rmu_sc0023858.1_g000001 Rmu_sc0029314.1_g000001
rosa_roxburghii Rroxscaffold_178G00437500 Rroxscaffold_178G00437540 Rroxscaffold_3G00225100 Rroxscaffold_3G00225110 Rroxscaffold_3G00225140 Rroxscaffold_3G00225170 Rroxscaffold_3G00225190 Rroxscaffold_3G00229880 Rroxscaffold_3G00233730 Rroxscaffold_7G00205760 Rroxscaffold_7G00205780 Rroxscaffold_7G00205820 Rroxscaffold_7G00205860
rosa_rugosa Rorug03G0221800 Rorug03G0270500 Rorug05G0313300 Rorug05G0582400 Rorug05G0582500 Rorug07G0230200.1 Rorug07G0230300 Rorug07G0230400 Rorug07G0295100 Rorug07G0296900
rosa_samantha Rh6AG098800 Rh6AG098900 Rh6AG184000 Rh6BG090700 Rh6BG090900 Rh6CG087500 Rh6CG087600 Rh6DG081800 Rh6DG082000 Rh7AG373100 Rh7AG412900 Rh7AG413000 Rh7AG451100 Rh7AG451200 Rh7BG364900 Rh7BG422800 Rh7BG422900 Rh7CG391400 Rh7CG431300 Rh7CG431400 Rh7CG469300 Rh7CG470800 Rh7CG471300 Rh7CG471400 Rh7DG367900 Rh7DG368000 Rh7DG375800 Rh7DG408800 Rh7DG440500 Rh7DG440600
rosa_wichuraiana Rw0G007810 Rw6G008570 Rw6G008580 Rw7G031720 Rw7G034090 Rw7G037500 Rw7G037510 Rw7G037580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 710
AclWI GGATC 1 cut(s) 90
AcsI RAATTY 3 cut(s) 651, 737, 830
AcuI CTGAAG 4 cut(s) 392, 515, 542, 711
AfaI GTAC 2 cut(s) 172, 847
AfiI CCNNNNNNNGG 2 cut(s) 28, 587
AflIII ACRYGT 1 cut(s) 448
AgsI TTSAA 6 cut(s) 237, 319, 382, 545, 730, 974
AjuI GAANNNNNNNTTGG 2 cut(s) 866, 898
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 2 cut(s) 39, 1137
AlwI GGATC 1 cut(s) 90
AlwNI CAGNNNCTG 1 cut(s) 481
AoxI GGCC 2 cut(s) 577, 628
ApeKI GCWGC 3 cut(s) 490, 538, 1116
ApoI RAATTY 3 cut(s) 651, 737, 830
Asp700I GAANNNNTTC 2 cut(s) 193, 405
AspLEI GCGC 2 cut(s) 325, 1226
AspS9I GGNCC 1 cut(s) 578
AsuHPI GGTGA 2 cut(s) 314, 922
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 3 cut(s) 502, 525, 1103
BccI CCATC 3 cut(s) 293, 589, 856
BcgI CGANNNNNNTGC 2 cut(s) 925, 959
BcoDI GTCTC 2 cut(s) 39, 1137
BfaI CTAG 3 cut(s) 356, 392, 615
BfuAI ACCTGC 1 cut(s) 710
BisI GCNGC 3 cut(s) 491, 539, 1117
BlpI GCTNAGC 1 cut(s) 1120
BlsI GCNGC 3 cut(s) 492, 540, 1118
BmcAI AGTACT 1 cut(s) 847
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 2 cut(s) 966, 1176
BmsI GCATC 1 cut(s) 40
BplI GAGNNNNNCTC 2 cut(s) 740, 772
BpmI CTGGAG 1 cut(s) 107
Bpu10I CCTNAGC 2 cut(s) 128, 425
Bpu1102I GCTNAGC 1 cut(s) 1120
BpuEI CTTGAG 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 1244
BsaWI WCCGGW 1 cut(s) 976
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 587
Bse1I ACTGG 1 cut(s) 914
Bse3DI GCAATG 2 cut(s) 340, 625
BseGI GGATG 1 cut(s) 1103
BseLI CCNNNNNNNGG 2 cut(s) 28, 587
BseMI GCAATG 2 cut(s) 340, 625
BseMII CTCAG 5 cut(s) 45, 267, 819, 833, 978
BseNI ACTGG 1 cut(s) 914
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 3 cut(s) 502, 525, 1103
BsgI GTGCAG 1 cut(s) 1316
BshFI GGCC 2 cut(s) 579, 630
BsiHKAI GWGCWC 1 cut(s) 257
BsiSI CCGG 1 cut(s) 977
BslFI GGGAC 1 cut(s) 1118
BslI CCNNNNNNNGG 2 cut(s) 28, 587
BsmAI GTCTC 2 cut(s) 39, 1137
BsmBI CGTCTC 2 cut(s) 39, 1137
BsmFI GGGAC 1 cut(s) 1118
BsnI GGCC 2 cut(s) 579, 630
Bsp1286I GDGCHC 1 cut(s) 257
Bsp143I GATC 4 cut(s) 23, 82, 348, 696
Bsp1720I GCTNAGC 1 cut(s) 1120
BspANI GGCC 2 cut(s) 579, 630
BspCNI CTCAG 5 cut(s) 46, 268, 818, 834, 979
BspLI GGNNCC 2 cut(s) 966, 1176
BspMI ACCTGC 1 cut(s) 710
BspPI GGATC 1 cut(s) 90
BspQI GCTCTTC 1 cut(s) 117
BsrDI GCAATG 2 cut(s) 340, 625
BsrI ACTGG 1 cut(s) 914
BssMI GATC 4 cut(s) 23, 82, 348, 696
Bst4CI ACNGT 1 cut(s) 180
Bst6I CTCTTC 2 cut(s) 117, 687
BstBAI YACGTR 1 cut(s) 1244
BstC8I GCNNGC 1 cut(s) 1197
BstF5I GGATG 1 cut(s) 1103
BstHHI GCGC 2 cut(s) 325, 1226
BstKTI GATC 4 cut(s) 26, 85, 351, 699
BstMAI GTCTC 2 cut(s) 39, 1137
BstMBI GATC 4 cut(s) 23, 82, 348, 696
BstMWI GCNNNNNNNGC 2 cut(s) 487, 515
BstNSI RCATGY 1 cut(s) 452
BstV1I GCAGC 3 cut(s) 502, 525, 1103
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BsuRI GGCC 2 cut(s) 579, 630
BtsCI GGATG 1 cut(s) 1103
BtsIMutI CAGTG 2 cut(s) 892, 921
BveI ACCTGC 1 cut(s) 710
Cac8I GCNNGC 1 cut(s) 1197
CaiI CAGNNNCTG 1 cut(s) 481
CfoI GCGC 2 cut(s) 325, 1226
Cfr13I GGNCC 1 cut(s) 578
CseI GACGC 2 cut(s) 515, 756
Csp6I GTAC 2 cut(s) 171, 846
CviAII CATG 3 cut(s) 449, 759, 1211
CviQI GTAC 2 cut(s) 171, 846
DpnI GATC 4 cut(s) 25, 84, 350, 698
DpnII GATC 4 cut(s) 23, 82, 348, 696
Eam1104I CTCTTC 2 cut(s) 117, 687
EarI CTCTTC 2 cut(s) 117, 687
Eco147I AGGCCT 1 cut(s) 630
Eco57I CTGAAG 4 cut(s) 392, 515, 542, 711
Esp3I CGTCTC 2 cut(s) 39, 1137
FaeI CATG 3 cut(s) 452, 762, 1214
FaiI YATR 9 cut(s) 230, 251, 270, 365, 450, 760, 1212, 1250, 1259
FaqI GGGAC 1 cut(s) 1118
FatI CATG 3 cut(s) 448, 758, 1210
Fnu4HI GCNGC 3 cut(s) 491, 539, 1117
FokI GGATG 1 cut(s) 1110
Fsp4HI GCNGC 3 cut(s) 491, 539, 1117
FspBI CTAG 3 cut(s) 356, 392, 615
GlaI GCGC 2 cut(s) 324, 1225
GluI GCNGC 3 cut(s) 491, 539, 1117
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 2 cut(s) 579, 630
HapII CCGG 1 cut(s) 977
HgaI GACGC 2 cut(s) 515, 756
HhaI GCGC 2 cut(s) 325, 1226
Hin1II CATG 3 cut(s) 452, 762, 1214
Hin6I GCGC 2 cut(s) 323, 1224
HinP1I GCGC 2 cut(s) 323, 1224
HindIII AAGCTT 2 cut(s) 384, 507
HpaII CCGG 1 cut(s) 977
HphI GGTGA 2 cut(s) 314, 922
Hpy166II GTNNAC 1 cut(s) 97
Hpy188III TCNNGA 4 cut(s) 80, 86, 109, 608
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 1 cut(s) 950
HpyAV CCTTC 2 cut(s) 313, 724
HpyCH4III ACNGT 1 cut(s) 180
HpyCH4IV ACGT 2 cut(s) 173, 1243
HpyF10VI GCNNNNNNNGC 2 cut(s) 487, 515
HpySE526I ACGT 2 cut(s) 173, 1243
Hsp92II CATG 3 cut(s) 452, 762, 1214
HspAI GCGC 2 cut(s) 323, 1224
Kzo9I GATC 4 cut(s) 23, 82, 348, 696
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 3 cut(s) 260, 602, 964
Lsp1109I GCAGC 3 cut(s) 502, 525, 1103
LweI GCATC 1 cut(s) 40
MaeI CTAG 3 cut(s) 356, 392, 615
MaeII ACGT 2 cut(s) 173, 1243
MaeIII GTNAC 3 cut(s) 174, 773, 1244
MalI GATC 4 cut(s) 25, 84, 350, 698
MboI GATC 4 cut(s) 23, 82, 348, 696
MboII GAAGA 8 cut(s) 19, 82, 85, 134, 398, 508, 552, 704
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 257
MluCI AATT 8 cut(s) 137, 293, 314, 651, 737, 830, 881, 1203
MlyI GAGTC 5 cut(s) 98, 353, 620, 797, 1100
MmeI TCCRAC 1 cut(s) 588
MnlI CCTC 7 cut(s) 214, 217, 709, 736, 820, 982, 1281
MroXI GAANNNNTTC 2 cut(s) 193, 405
MseI TTAA 4 cut(s) 288, 333, 834, 1188
MslI CAYNNNNRTG 1 cut(s) 797
MspA1I CMGCKG 1 cut(s) 493
MspI CCGG 1 cut(s) 977
MwoI GCNNNNNNNGC 2 cut(s) 487, 515
NdeII GATC 4 cut(s) 23, 82, 348, 696
NlaIII CATG 3 cut(s) 452, 762, 1214
NlaIV GGNNCC 2 cut(s) 966, 1176
NmuCI GTSAC 2 cut(s) 773, 1244
NspI RCATGY 1 cut(s) 452
PceI AGGCCT 1 cut(s) 630
PciI ACATGT 1 cut(s) 448
PciSI GCTCTTC 1 cut(s) 117
PcsI WCGNNNNNNNCGW 1 cut(s) 170
PdmI GAANNNNTTC 2 cut(s) 193, 405
PfeI GAWTC 5 cut(s) 32, 76, 604, 623, 953
PkrI GCNGC 3 cut(s) 492, 540, 1118
PleI GAGTC 5 cut(s) 97, 353, 619, 797, 1100
PpsI GAGTC 5 cut(s) 97, 353, 619, 797, 1100
Ppu21I YACGTR 1 cut(s) 1244
PscI ACATGT 1 cut(s) 448
PspN4I GGNNCC 2 cut(s) 966, 1176
PspPI GGNCC 1 cut(s) 578
PstNI CAGNNNCTG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 2 cut(s) 172, 847
RsaNI GTAC 2 cut(s) 171, 846
RseI CAYNNNNRTG 1 cut(s) 797
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 4 cut(s) 288, 333, 834, 1188
SatI GCNGC 3 cut(s) 491, 539, 1117
Sau3AI GATC 4 cut(s) 23, 82, 348, 696
Sau96I GGNCC 1 cut(s) 578
ScaI AGTACT 1 cut(s) 847
SchI GAGTC 5 cut(s) 98, 353, 620, 797, 1100
SduI GDGCHC 1 cut(s) 257
SfaNI GCATC 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 797
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse9I AATT 8 cut(s) 137, 293, 314, 651, 737, 830, 881, 1203
SseBI AGGCCT 1 cut(s) 630
SspMI CTAG 3 cut(s) 356, 392, 615
StuI AGGCCT 1 cut(s) 630
TaaI ACNGT 1 cut(s) 180
TaiI ACGT 2 cut(s) 176, 1246
TaqI TCGA 2 cut(s) 152, 945
TasI AATT 8 cut(s) 137, 293, 314, 651, 737, 830, 881, 1203
TatI WGTACW 1 cut(s) 845
TfiI GAWTC 5 cut(s) 32, 76, 604, 623, 953
Tru1I TTAA 4 cut(s) 288, 333, 834, 1188
Tru9I TTAA 4 cut(s) 288, 333, 834, 1188
TscAI CASTG 2 cut(s) 892, 921
TseFI GTSAC 2 cut(s) 773, 1244
TseI GCWGC 3 cut(s) 490, 538, 1116
Tsp45I GTSAC 2 cut(s) 773, 1244
TspDTI ATGAA 6 cut(s) 24, 636, 644, 653, 692, 966
TspGWI ACGGA 1 cut(s) 82
TspRI CASTG 2 cut(s) 892, 921
XapI RAATTY 3 cut(s) 651, 737, 830
XceI RCATGY 1 cut(s) 452
XmnI GAANNNNTTC 2 cut(s) 193, 405
XspI CTAG 3 cut(s) 356, 392, 615
ZrmI AGTACT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.