Rroxscaffold_3G00225140

Chromosome-associated kinesin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000316
Physical Location & Seq
Forward (+)
8356747 .. 8361930
5184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225140.1

Sequence Viewer

Length: 1323 bp
ATGCATGTGGATTGTAAGGAGGTTGAGGAACTGAGATACAAGCTTGCAAATGTTTCATCTACTAGTGGTGTGGAGAACAGTGCTCAGAAACTCAGAGAAAACTACCTGCAGAAGTTGACTTTCCTCGAAGATCAGGTTACGGTGTTGACGAGGAAGCTAGATGCTCAATCTCAACTCTCAACCCAAAGAAGAAGAGGGGACGAGTCAGCAAAGCCGTTCCAGTTTGAGATTCAGAGATTGAAGGCTCAGAAGGTTCAAATGCAATGTAAGATGAAACTAGAATCTGTGCAGTTCAGATTGCATAAAGCTTTGTTGGACAAGGAAGTTCTTCAGCTCAAGAAAGAGAATAGGAAGAATAAACATGAGATGCACAAGCTATTGGCCTCCAATCAAAGACTGACGACTGTTTTGCGTCGAAAGACTGAAGAAGCATCTGTGGCTACTAAACAGCTGAGACGGCTCTTAGAATCTCGCAAGGCTTTGTTGCTAAAAAGAGGTGGCAAAAATGGGAATAATACAGCAACTCAGATCACCTCTCGTTTTGGAATGTCTAGGATGGTGGAGGAGGCTGAAAAGCTTAAAGATGAAGTAGAGGCACTGCAACAAGAAAAGTCAAGGTGCTCATGCCAGGAGAAAGAGGTTGACAGCTTTGAGAAGGATTTAGATATAACAGACCTGAAGGCTCAAGTAGTCAGCCTCAGTAGTATGGTTGAACAATTAAGATTGCGCAAGGCTGAGCTTGATCATGGGAAGTCACAGGGCATTAGGAGTCAGCATACTGCATCTGTTGGGAGCAGTTACAAGTTAGTGGAGGACATTAGTCCATCTGCATCAGAAAATTCCACAGTTGGAACATCTAAACCGGCAGCTCCAGTTTGCTGCTCATGCACTAAGAATTCTTTGTGCAAGACGATGAAATGCAAATGTCGATCCAAGGGTGGAAGCTGTGGTGCCTCATGTGGCTGTGCAGCATCCAAGTGCAGCAATAGGAAAGCAGTCCCAATTAAGTCGAGTGACTCGCCACTATCAGAGATTGCTAATGGCGTTCTGAATAGTTCAAACACCAGTGAAACAGTGAAGAGTAGCATAGAGGCTTCTGAAGGTGCAATGCTACTTCAGAGTGCACTAGTTCAGAAGCCTGCTGAACCGGAAGGGAACTTTGGAGCAATAAAGAAACCCTTAACTGAAATTGGGAACATATTGGTGAGTACAAATGCTGCAAAACCTGGCCCAAGAAAAAAGGGGAGAAAGCCAGCGATTCAGCTTGTTACTGTAGACCCAATTTCCTCAGTGCCAGTAAATATGGAAGGAAATGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

440

Amino Acids

48.44

Weight (kDa)

9.49

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIF21A_4th PF25764 6 - 115 2.6e-22 KIF21A-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G33300
fragaria_vesca FvH4_3g26472 FvH4_5g30650 FvH4_5g30650 FvH4_5g30650 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250
malus_domestica MD08G1198100.v1.1 MD08G1222000.v1.1 MD15G1385400.v1.1 MD15G1440800.v1.1
prunus_persica Prupe.1G532100_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1
pyrus_communis pycom08g17030 pycom08g17040 pycom08g19270 pycom08g19280 pycom15g34550 pycom15g36860 pycom15g38860
rosa_chinensis RchiOBHm_Chr6g0258571 RchiOBHm_Chr6g0258581 RchiOBHm_Chr6g0258621 RchiOBHm_Chr7g0225511 RchiOBHm_Chr7g0230561 RchiOBHm_Chr7g0230571 RchiOBHm_Chr7g0236371 RchiOBHm_Chr7g0236391
rosa_laevigata RLG00000001080 RLG00000001810 RLG00000014605
rosa_multiflora Rmu_co8399019.1_g000001 Rmu_sc0002222.1_g000016 Rmu_sc0002449.1_g000032 Rmu_sc0003720.1_g000005 Rmu_sc0005578.1_g000003 Rmu_sc0011035.1_g000002 Rmu_sc0012681.1_g000001 Rmu_sc0023362.1_g000001 Rmu_sc0023858.1_g000001 Rmu_sc0029314.1_g000001
rosa_roxburghii Rroxscaffold_178G00437500 Rroxscaffold_178G00437540 Rroxscaffold_3G00225100 Rroxscaffold_3G00225110 Rroxscaffold_3G00225140 Rroxscaffold_3G00225170 Rroxscaffold_3G00225190 Rroxscaffold_3G00229880 Rroxscaffold_3G00233730 Rroxscaffold_7G00205760 Rroxscaffold_7G00205780 Rroxscaffold_7G00205820 Rroxscaffold_7G00205860
rosa_rugosa Rorug03G0221800 Rorug03G0270500 Rorug05G0313300 Rorug05G0582400 Rorug05G0582500 Rorug07G0230200.1 Rorug07G0230300 Rorug07G0230400 Rorug07G0295100 Rorug07G0296900
rosa_samantha Rh6AG098800 Rh6AG098900 Rh6AG184000 Rh6BG090700 Rh6BG090900 Rh6CG087500 Rh6CG087600 Rh6DG081800 Rh6DG082000 Rh7AG373100 Rh7AG412900 Rh7AG413000 Rh7AG451100 Rh7AG451200 Rh7BG364900 Rh7BG422800 Rh7BG422900 Rh7CG391400 Rh7CG431300 Rh7CG431400 Rh7CG469300 Rh7CG470800 Rh7CG471300 Rh7CG471400 Rh7DG367900 Rh7DG368000 Rh7DG375800 Rh7DG408800 Rh7DG440500 Rh7DG440600
rosa_wichuraiana Rw0G007810 Rw6G008570 Rw6G008580 Rw7G031720 Rw7G034090 Rw7G037500 Rw7G037510 Rw7G037580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 728
Acc36I ACCTGC 1 cut(s) 114
AccB1I GGYRCC 1 cut(s) 950
AccI GTMKAC 1 cut(s) 1275
AclWI GGATC 1 cut(s) 924
AcsI RAATTY 2 cut(s) 838, 895
AcuI CTGAAG 5 cut(s) 314, 444, 698, 1100, 1119
AfaI GTAC 1 cut(s) 1210
AgsI TTSAA 4 cut(s) 241, 257, 713, 1059
AhlI ACTAGT 2 cut(s) 62, 1126
AjnI CCWGG 2 cut(s) 627, 1225
AjuI GAANNNNNNNTTGG 2 cut(s) 1143, 1175
Alw21I GWGCWC 3 cut(s) 85, 623, 1126
Alw26I GTCTC 1 cut(s) 448
Alw44I GTGCAC 1 cut(s) 1122
AlwI GGATC 1 cut(s) 924
AoxI GGCC 2 cut(s) 381, 1228
ApaLI GTGCAC 1 cut(s) 1122
ApeKI GCWGC 5 cut(s) 866, 879, 968, 981, 1217
ApoI RAATTY 2 cut(s) 838, 895
ArsI GACNNNNNNTTYG 2 cut(s) 391, 423
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 729
AspS9I GGNCC 1 cut(s) 1229
AsuHPI GGTGA 2 cut(s) 523, 1216
BaeGI GKGCMC 1 cut(s) 1126
BanI GGYRCC 1 cut(s) 950
Bbv12I GWGCWC 3 cut(s) 85, 623, 1126
BbvI GCAGC 5 cut(s) 866, 878, 980, 993, 1204
BccI CCATC 2 cut(s) 550, 832
BceAI ACGGC 2 cut(s) 199, 473
BcgI CGANNNNNNTGC 2 cut(s) 391, 425
BciT130I CCWGG 2 cut(s) 629, 1227
BclI TGATCA 1 cut(s) 742
BcoDI GTCTC 1 cut(s) 448
BcuI ACTAGT 2 cut(s) 62, 1126
BfaI CTAG 5 cut(s) 63, 158, 278, 552, 1127
BfmI CTRYAG 2 cut(s) 107, 1272
BfuAI ACCTGC 1 cut(s) 114
BisI GCNGC 5 cut(s) 867, 880, 969, 982, 1218
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 5 cut(s) 868, 881, 970, 983, 1219
Bme1390I CCNGG 2 cut(s) 629, 1227
BmgT120I GGNCC 1 cut(s) 1229
BmiI GGNNCC 1 cut(s) 952
BmrFI CCNGG 2 cut(s) 629, 1227
BmsI GCATC 6 cut(s) 151, 357, 440, 791, 839, 980
BoxI GACNNNNGTC 1 cut(s) 819
BpmI CTGGAG 1 cut(s) 855
Bpu1102I GCTNAGC 1 cut(s) 735
BpuEI CTTGAG 2 cut(s) 320, 669
BsaJI CCNNGG 1 cut(s) 933
BsaWI WCCGGW 1 cut(s) 1147
Bse118I RCCGGY 1 cut(s) 862
Bse1I ACTGG 4 cut(s) 220, 872, 1065, 1295
Bse3DI GCAATG 2 cut(s) 269, 1113
BseBI CCWGG 2 cut(s) 629, 1227
BseDI CCNNGG 1 cut(s) 933
BseGI GGATG 2 cut(s) 561, 971
BseMI GCAATG 2 cut(s) 269, 1113
BseMII CTCAG 9 cut(s) 23, 98, 106, 260, 443, 539, 712, 726, 1302
BseNI ACTGG 4 cut(s) 220, 872, 1065, 1295
BseRI GAGGAG 1 cut(s) 578
BseSI GKGCMC 1 cut(s) 1126
BseXI GCAGC 5 cut(s) 866, 878, 980, 993, 1204
BsgI GTGCAG 3 cut(s) 308, 987, 1000
BshFI GGCC 2 cut(s) 383, 1230
BshNI GGYRCC 1 cut(s) 950
BsiHKAI GWGCWC 3 cut(s) 85, 623, 1126
BsiSI CCGG 2 cut(s) 863, 1148
BslFI GGGAC 2 cut(s) 212, 983
BsmAI GTCTC 1 cut(s) 448
BsmBI CGTCTC 1 cut(s) 448
BsmFI GGGAC 2 cut(s) 212, 983
BsnI GGCC 2 cut(s) 383, 1230
Bsp1286I GDGCHC 3 cut(s) 85, 623, 1126
Bsp143I GATC 4 cut(s) 130, 528, 742, 929
Bsp1720I GCTNAGC 1 cut(s) 735
BspANI GGCC 2 cut(s) 383, 1230
BspCNI CTCAG 9 cut(s) 24, 97, 105, 259, 444, 538, 711, 727, 1301
BspLI GGNNCC 1 cut(s) 952
BspMAI CTGCAG 1 cut(s) 111
BspMI ACCTGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 924
BspT107I GGYRCC 1 cut(s) 950
BsrDI GCAATG 2 cut(s) 269, 1113
BsrFI RCCGGY 1 cut(s) 862
BsrI ACTGG 4 cut(s) 220, 872, 1065, 1295
BssAI RCCGGY 1 cut(s) 862
BssECI CCNNGG 1 cut(s) 933
BssMI GATC 4 cut(s) 130, 528, 742, 929
BssT1I CCWWGG 1 cut(s) 933
Bst2UI CCWGG 2 cut(s) 629, 1227
Bst4CI ACNGT 6 cut(s) 80, 142, 406, 847, 1075, 1273
Bst6I CTCTTC 2 cut(s) 187, 1073
BstC8I GCNNGC 3 cut(s) 45, 1140, 1254
BstF5I GGATG 2 cut(s) 561, 971
BstHHI GCGC 1 cut(s) 729
BstKTI GATC 4 cut(s) 133, 531, 745, 932
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 4 cut(s) 130, 528, 742, 929
BstMWI GCNNNNNNNGC 3 cut(s) 437, 457, 885
BstNI CCWGG 2 cut(s) 629, 1227
BstNSI RCATGY 1 cut(s) 8
BstPAI GACNNNNGTC 1 cut(s) 819
BstSCI CCNGG 2 cut(s) 627, 1225
BstSFI CTRYAG 2 cut(s) 107, 1272
BstSLI GKGCMC 1 cut(s) 1126
BstV1I GCAGC 5 cut(s) 866, 878, 980, 993, 1204
BsuRI GGCC 2 cut(s) 383, 1230
BtsCI GGATG 2 cut(s) 561, 971
BtsI GCAGTG 1 cut(s) 596
BtsIMutI CAGTG 5 cut(s) 85, 596, 1072, 1080, 1296
BveI ACCTGC 1 cut(s) 114
Cac8I GCNNGC 3 cut(s) 45, 1140, 1254
CfoI GCGC 1 cut(s) 729
Cfr10I RCCGGY 1 cut(s) 862
Cfr13I GGNCC 1 cut(s) 1229
CseI GACGC 1 cut(s) 401
Csp6I GTAC 1 cut(s) 1209
CviAII CATG 6 cut(s) 5, 362, 624, 746, 885, 957
CviQI GTAC 1 cut(s) 1209
DpnI GATC 4 cut(s) 132, 530, 744, 931
DpnII GATC 4 cut(s) 130, 528, 742, 929
Eam1104I CTCTTC 2 cut(s) 187, 1073
EarI CTCTTC 2 cut(s) 187, 1073
Eco130I CCWWGG 1 cut(s) 933
Eco57I CTGAAG 5 cut(s) 314, 444, 698, 1100, 1119
EcoRI GAATTC 1 cut(s) 895
EcoRII CCWGG 2 cut(s) 627, 1225
EcoT14I CCWWGG 1 cut(s) 933
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 933
Esp3I CGTCTC 1 cut(s) 448
FaeI CATG 6 cut(s) 8, 365, 627, 749, 888, 960
FalI AAGNNNNNCTT 2 cut(s) 1163, 1195
FaqI GGGAC 2 cut(s) 212, 983
FatI CATG 6 cut(s) 4, 361, 623, 745, 884, 956
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 1 cut(s) 1275
Fnu4HI GCNGC 5 cut(s) 867, 880, 969, 982, 1218
FokI GGATG 2 cut(s) 568, 958
Fsp4HI GCNGC 5 cut(s) 867, 880, 969, 982, 1218
FspBI CTAG 5 cut(s) 63, 158, 278, 552, 1127
FspI TGCGCA 1 cut(s) 728
GlaI GCGC 1 cut(s) 728
GluI GCNGC 5 cut(s) 867, 880, 969, 982, 1218
GsuI CTGGAG 1 cut(s) 855
HaeIII GGCC 2 cut(s) 383, 1230
HapII CCGG 2 cut(s) 863, 1148
HgaI GACGC 1 cut(s) 401
HhaI GCGC 1 cut(s) 729
Hin1II CATG 6 cut(s) 8, 365, 627, 749, 888, 960
Hin6I GCGC 1 cut(s) 727
HinP1I GCGC 1 cut(s) 727
HincII GTYRAC 3 cut(s) 117, 147, 643
HindII GTYRAC 3 cut(s) 117, 147, 643
HindIII AAGCTT 3 cut(s) 41, 306, 575
HinfI GANTC 7 cut(s) 203, 229, 281, 467, 769, 1016, 1258
HpaII CCGG 2 cut(s) 863, 1148
HphI GGTGA 2 cut(s) 523, 1216
Hpy166II GTNNAC 5 cut(s) 117, 147, 643, 1124, 1276
Hpy188III TCNNGA 1 cut(s) 337
Hpy8I GTNNAC 5 cut(s) 117, 147, 643, 1124, 1276
Hpy99I CGWCG 1 cut(s) 417
HpyAV CCTTC 7 cut(s) 235, 244, 649, 673, 1094, 1145, 1301
HpyCH4III ACNGT 6 cut(s) 80, 142, 406, 847, 1075, 1273
HpyF10VI GCNNNNNNNGC 3 cut(s) 437, 457, 885
Hsp92II CATG 6 cut(s) 8, 365, 627, 749, 888, 960
HspAI GCGC 1 cut(s) 727
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 4 cut(s) 130, 528, 742, 929
LmnI GCTCC 3 cut(s) 792, 874, 1163
Lsp1109I GCAGC 5 cut(s) 866, 878, 980, 993, 1204
LweI GCATC 6 cut(s) 151, 357, 440, 791, 839, 980
MaeI CTAG 5 cut(s) 63, 158, 278, 552, 1127
MaeIII GTNAC 5 cut(s) 136, 753, 797, 1013, 1267
MalI GATC 4 cut(s) 132, 530, 744, 931
MboI GATC 4 cut(s) 130, 528, 742, 929
MboII GAAGA 7 cut(s) 140, 201, 204, 320, 364, 437, 1090
MhlI GDGCHC 3 cut(s) 85, 623, 1126
MluCI AATT 6 cut(s) 716, 838, 895, 1002, 1188, 1281
MlyI GAGTC 3 cut(s) 212, 778, 1010
MmeI TCCRAC 2 cut(s) 294, 829
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 4 cut(s) 579, 719, 1005, 1181
MslI CAYNNNNRTG 2 cut(s) 976, 1202
MspA1I CMGCKG 1 cut(s) 451
MspI CCGG 2 cut(s) 863, 1148
MspR9I CCNGG 2 cut(s) 629, 1227
MvaI CCWGG 2 cut(s) 629, 1227
MwoI GCNNNNNNNGC 3 cut(s) 437, 457, 885
NdeII GATC 4 cut(s) 130, 528, 742, 929
NlaIII CATG 6 cut(s) 8, 365, 627, 749, 888, 960
NlaIV GGNNCC 1 cut(s) 952
NmuCI GTSAC 2 cut(s) 753, 1013
NsbI TGCGCA 1 cut(s) 728
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 4 cut(s) 229, 281, 467, 1258
PkrI GCNGC 5 cut(s) 868, 881, 970, 983, 1219
PleI GAGTC 3 cut(s) 211, 777, 1010
PpsI GAGTC 3 cut(s) 211, 777, 1010
PshAI GACNNNNGTC 1 cut(s) 819
Psp6I CCWGG 2 cut(s) 627, 1225
PspGI CCWGG 2 cut(s) 627, 1225
PspN4I GGNNCC 1 cut(s) 952
PspPI GGNCC 1 cut(s) 1229
PstI CTGCAG 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 451
RsaI GTAC 1 cut(s) 1210
RsaNI GTAC 1 cut(s) 1209
RseI CAYNNNNRTG 2 cut(s) 976, 1202
SaqAI TTAA 4 cut(s) 579, 719, 1005, 1181
SatI GCNGC 5 cut(s) 867, 880, 969, 982, 1218
Sau3AI GATC 4 cut(s) 130, 528, 742, 929
Sau96I GGNCC 1 cut(s) 1229
SchI GAGTC 3 cut(s) 212, 778, 1010
ScrFI CCNGG 2 cut(s) 629, 1227
SduI GDGCHC 3 cut(s) 85, 623, 1126
SfaNI GCATC 6 cut(s) 151, 357, 440, 791, 839, 980
SfcI CTRYAG 2 cut(s) 107, 1272
SmiMI CAYNNNNRTG 2 cut(s) 976, 1202
SmlI CTYRAG 2 cut(s) 335, 684
SmoI CTYRAG 2 cut(s) 335, 684
SpeI ACTAGT 2 cut(s) 62, 1126
Sse9I AATT 6 cut(s) 716, 838, 895, 1002, 1188, 1281
SspMI CTAG 5 cut(s) 63, 158, 278, 552, 1127
StyD4I CCNGG 2 cut(s) 627, 1225
StyI CCWWGG 1 cut(s) 933
TaaI ACNGT 6 cut(s) 80, 142, 406, 847, 1075, 1273
TaqI TCGA 4 cut(s) 126, 415, 928, 1010
TasI AATT 6 cut(s) 716, 838, 895, 1002, 1188, 1281
TatI WGTACW 1 cut(s) 1208
TfiI GAWTC 4 cut(s) 229, 281, 467, 1258
Tru1I TTAA 4 cut(s) 579, 719, 1005, 1181
Tru9I TTAA 4 cut(s) 579, 719, 1005, 1181
TscAI CASTG 5 cut(s) 85, 603, 1072, 1080, 1296
TseFI GTSAC 2 cut(s) 753, 1013
TseI GCWGC 5 cut(s) 866, 879, 968, 981, 1217
Tsp45I GTSAC 2 cut(s) 753, 1013
TspDTI ATGAA 4 cut(s) 45, 287, 600, 929
TspRI CASTG 5 cut(s) 85, 603, 1072, 1080, 1296
VneI GTGCAC 1 cut(s) 1122
XapI RAATTY 2 cut(s) 838, 895
XceI RCATGY 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 1275
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 5 cut(s) 63, 158, 278, 552, 1127
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.