FvH4_5g30650

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000316
Physical Location & Seq
Forward (+)
21549228 .. 21555537
6310 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30650.t2

Sequence Viewer

Length: 1527 bp
ATGGGTTTAGATAGTGTGCAAGGGATGAGGAACAAGAATACGAAGAGGAAGGCGGTGGAGGTGGAGGTGGAGGTGGAGAAGGATTCAGAGGACTGGTGCTTCCAATGCAAAGATGGTGGAGATCTTCTTATTTGCGATTACAAGGATTGTTTAAAAGTTTATCATCCGGTATGCGTGGGGAAAAAGAAAACCTTCATGAACTCTGGAAGGCACTGGACTTGTAGTAGGCATTCTTGTTCAGTGTGCAGTAGTACTCCAAGCTTTTATTGCCTTTGCTGTCCAAATTCATCAATATGTAGAGATTGCTTTAGTGCTTCTGAGTTTACACTGCTAAAAGGGAATAATGGCTTGTGCAAGTGTTGTTTAAAGCTTATTCTGCTTGCAGAAGAAAACGCTGAATACGGTCTAGAAGGAGAGAAAATAGACTTCAACAATGGTGATACTTTTGAGTGCCTATTTAAGCAGTGTTGGGAAATTTTGAAGGAACAAGAAGGTTTCACTTTGGATGATGTCTATTCTGCATATGCCACATTAAAGGGAACAAATTATAATGGCAGATTTGATTCAGTAAATATTGTTGATAGTGACGGAAGTAAGGACCTGACAACATCAGACTGTGAGACTAGTGCCATAGAGTTTCAGAAGACAGTAGGAACAAAGAAGAGATTTCAGCCAATGGAATTTAAAGGATGGGGTTCCAAACCACTCATTGAATTCCTGAGATCAATAGGGAAGGACACAACCAAGAAGTTATCCCGGTTTGATGTGGAGTGTATCATCTCTTATTACATCAAGGATAACAACCTCCCAAAACAGAAGAAAATGGTTCAATGTGATAATAATCTGTATTCCATCTTCAAACAGAAATCCATCAATACGACAAAATTATATCAACTTCTGGAGGAACATTTTGCAGAAATTACTGTAATAGAGGACAGTGGAAATCAGGATGAAGATGAAATCAGTTTGGAAGATAAGAACAAAGGTACAAAGAGCAAGAAGAGAAGAGTTATGAGCTCAGATGTCATAACAACTGATGAAATCAAAAGTCCTAGCATACATAAAAGCTGCTTTGCATCCATTATTGCTGAGAATATGAAGCTTGTTTACATAAGGAGGAGCCTAGTAGAAGATTTTTTGAAGGAGCCCTATCATTCAGAAAGCAAGATACTGGGGAGTTTCGTGAGAGTGAAAAATGACCCTCGAGACTATCTTCTGAGAAATAGTCACCAGCTTTCACAAGTTAAAGGCATGAAGAAAATCACGACAACTAATGGAGCAGGCTTCGAAATTCTCCTGCAAGTTTCCTGTTGTACAAAAGATATTCCCATTTCTTTGCTAGCAGATACTGACTTCACTCAGGAAGAATGTGAAGATTTGCGTCAACACATCGCCAAAGGCTTGCTAGAGAAACCTACAGTCGCTGCGCTTCAAGAGAAGGCAAAAGTTCTCCATGAGGACATAACTAAGCATGCAAGTATCTCTAACTCTTTGCCTATTGACATATTTACGTGTAACCTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plus-3 SWIB

Protein Analysis

509

Amino Acids

57.65

Weight (kDa)

6.14

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 233 - 304 6.1e-10 SWIB/MDM2 domain
Plus-3 PF03126 365 - 464 9.6e-14 Plus-3 domain
NERD_plant PF25980 486 - 535 5.6e-22 Plant NERD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G33300
fragaria_vesca FvH4_3g26472 FvH4_5g30650 FvH4_5g30650 FvH4_5g30650 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250
malus_domestica MD08G1198100.v1.1 MD08G1222000.v1.1 MD15G1385400.v1.1 MD15G1440800.v1.1
prunus_persica Prupe.1G532100_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1
pyrus_communis pycom08g17030 pycom08g17040 pycom08g19270 pycom08g19280 pycom15g34550 pycom15g36860 pycom15g38860
rosa_chinensis RchiOBHm_Chr6g0258571 RchiOBHm_Chr6g0258581 RchiOBHm_Chr6g0258621 RchiOBHm_Chr7g0225511 RchiOBHm_Chr7g0230561 RchiOBHm_Chr7g0230571 RchiOBHm_Chr7g0236371 RchiOBHm_Chr7g0236391
rosa_laevigata RLG00000001080 RLG00000001810 RLG00000014605
rosa_multiflora Rmu_co8399019.1_g000001 Rmu_sc0002222.1_g000016 Rmu_sc0002449.1_g000032 Rmu_sc0003720.1_g000005 Rmu_sc0005578.1_g000003 Rmu_sc0011035.1_g000002 Rmu_sc0012681.1_g000001 Rmu_sc0023362.1_g000001 Rmu_sc0023858.1_g000001 Rmu_sc0029314.1_g000001
rosa_roxburghii Rroxscaffold_178G00437500 Rroxscaffold_178G00437540 Rroxscaffold_3G00225100 Rroxscaffold_3G00225110 Rroxscaffold_3G00225140 Rroxscaffold_3G00225170 Rroxscaffold_3G00225190 Rroxscaffold_3G00229880 Rroxscaffold_3G00233730 Rroxscaffold_7G00205760 Rroxscaffold_7G00205780 Rroxscaffold_7G00205820 Rroxscaffold_7G00205860
rosa_rugosa Rorug03G0221800 Rorug03G0270500 Rorug05G0313300 Rorug05G0582400 Rorug05G0582500 Rorug07G0230200.1 Rorug07G0230300 Rorug07G0230400 Rorug07G0295100 Rorug07G0296900
rosa_samantha Rh6AG098800 Rh6AG098900 Rh6AG184000 Rh6BG090700 Rh6BG090900 Rh6CG087500 Rh6CG087600 Rh6DG081800 Rh6DG082000 Rh7AG373100 Rh7AG412900 Rh7AG413000 Rh7AG451100 Rh7AG451200 Rh7BG364900 Rh7BG422800 Rh7BG422900 Rh7CG391400 Rh7CG431300 Rh7CG431400 Rh7CG469300 Rh7CG470800 Rh7CG471300 Rh7CG471400 Rh7DG367900 Rh7DG368000 Rh7DG375800 Rh7DG408800 Rh7DG440500 Rh7DG440600
rosa_wichuraiana Rw0G007810 Rw6G008570 Rw6G008580 Rw7G031720 Rw7G034090 Rw7G037500 Rw7G037510 Rw7G037580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
AciI CCGC 1 cut(s) 53
AcsI RAATTY 5 cut(s) 283, 474, 680, 713, 1290
AfaI GTAC 3 cut(s) 253, 988, 1315
AflIII ACRYGT 1 cut(s) 1511
AgsI TTSAA 7 cut(s) 430, 481, 713, 830, 859, 1141, 1433
AhlI ACTAGT 1 cut(s) 623
AluBI AGCT 6 cut(s) 261, 370, 1017, 1068, 1102, 1234
AluI AGCT 6 cut(s) 261, 370, 1017, 1068, 1102, 1234
Alw21I GWGCWC 1 cut(s) 1019
Alw26I GTCTC 2 cut(s) 614, 1200
AlwNI CAGNNNCTG 2 cut(s) 1349, 1424
Ama87I CYCGRG 1 cut(s) 1203
ApeKI GCWGC 2 cut(s) 1068, 1424
ApoI RAATTY 5 cut(s) 283, 474, 680, 713, 1290
Asp700I GAANNNNTTC 1 cut(s) 191
AspLEI GCGC 1 cut(s) 1429
AspS9I GGNCC 1 cut(s) 598
AsuC2I CCSGG 1 cut(s) 757
AsuHPI GGTGA 2 cut(s) 449, 1220
AsuII TTCGAA 1 cut(s) 1287
AsuNHI GCTAGC 1 cut(s) 1339
AvaI CYCGRG 1 cut(s) 1203
AvaII GGWCC 1 cut(s) 598
BanII GRGCYC 2 cut(s) 1019, 1149
BarI GAAGNNNNNNTAC 4 cut(s) 839, 871, 1091, 1123
BbsI GAAGAC 1 cut(s) 650
Bbv12I GWGCWC 1 cut(s) 1019
BbvI GCAGC 2 cut(s) 1055, 1411
BccI CCATC 4 cut(s) 107, 684, 860, 878
BcnI CCSGG 1 cut(s) 757
BcoDI GTCTC 2 cut(s) 614, 1200
BcuI ACTAGT 1 cut(s) 623
BfaI CTAG 6 cut(s) 407, 624, 1053, 1124, 1340, 1406
BfmI CTRYAG 1 cut(s) 1416
BglII AGATCT 1 cut(s) 121
BisI GCNGC 2 cut(s) 1069, 1425
BlsI GCNGC 2 cut(s) 1070, 1426
BmcAI AGTACT 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 757
Bme18I GGWCC 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 1203
BmgT120I GGNCC 1 cut(s) 598
BmiI GGNNCC 3 cut(s) 697, 1121, 1146
BmrFI CCNGG 1 cut(s) 757
BmrI ACTGGG 1 cut(s) 1181
BmsI GCATC 1 cut(s) 1085
BmtI GCTAGC 1 cut(s) 1343
BmuI ACTGGG 1 cut(s) 1181
BpiI GAAGAC 1 cut(s) 650
BpmI CTGGAG 1 cut(s) 920
Bpu14I TTCGAA 1 cut(s) 1287
BpuMI CCSGG 1 cut(s) 757
BsaAI YACGTR 1 cut(s) 1512
BsaBI GATNNNNATC 1 cut(s) 840
BsaWI WCCGGW 1 cut(s) 166
Bse1I ACTGG 3 cut(s) 98, 218, 1176
Bse8I GATNNNNATC 1 cut(s) 840
BseGI GGATG 6 cut(s) 30, 163, 511, 695, 955, 1076
BseJI GATNNNNATC 1 cut(s) 840
BseMII CTCAG 6 cut(s) 309, 710, 1032, 1080, 1208, 1373
BseNI ACTGG 3 cut(s) 98, 218, 1176
BseRI GAGGAG 1 cut(s) 1132
BseXI GCAGC 2 cut(s) 1055, 1411
BsgI GTGCAG 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 1019
BsiHKCI CYCGRG 1 cut(s) 1203
BsiSI CCGG 2 cut(s) 167, 757
BsmAI GTCTC 2 cut(s) 614, 1200
BsmI GAATGC 1 cut(s) 229
BsoBI CYCGRG 1 cut(s) 1203
Bsp119I TTCGAA 1 cut(s) 1287
Bsp1286I GDGCHC 2 cut(s) 1019, 1149
Bsp1407I TGTACA 1 cut(s) 1313
Bsp143I GATC 2 cut(s) 121, 722
BspACI CCGC 1 cut(s) 53
BspCNI CTCAG 6 cut(s) 310, 711, 1031, 1081, 1209, 1372
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 3 cut(s) 697, 1121, 1146
BspOI GCTAGC 1 cut(s) 1343
BspT104I TTCGAA 1 cut(s) 1287
BsrGI TGTACA 1 cut(s) 1313
BsrI ACTGG 3 cut(s) 98, 218, 1176
BssMI GATC 2 cut(s) 121, 722
Bst4CI ACNGT 6 cut(s) 404, 617, 649, 925, 938, 1420
Bst6I CTCTTC 4 cut(s) 38, 656, 995, 1000
BstAUI TGTACA 1 cut(s) 1313
BstBAI YACGTR 1 cut(s) 1512
BstBI TTCGAA 1 cut(s) 1287
BstC8I GCNNGC 5 cut(s) 381, 1282, 1341, 1403, 1473
BstDEI CTNAG 7 cut(s) 318, 719, 1018, 1089, 1217, 1359, 1467
BstF5I GGATG 6 cut(s) 30, 163, 511, 695, 955, 1076
BstHHI GCGC 1 cut(s) 1429
BstKTI GATC 2 cut(s) 124, 725
BstMAI GTCTC 2 cut(s) 614, 1200
BstMBI GATC 2 cut(s) 121, 722
BstMWI GCNNNNNNNGC 3 cut(s) 105, 267, 376
BstNSI RCATGY 1 cut(s) 1475
BstSCI CCNGG 1 cut(s) 755
BstSFI CTRYAG 1 cut(s) 1416
BstV1I GCAGC 2 cut(s) 1055, 1411
BstV2I GAAGAC 1 cut(s) 650
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BtgZI GCGATG 1 cut(s) 1375
BtsCI GGATG 6 cut(s) 30, 163, 511, 695, 955, 1076
BtsI GCAGTG 2 cut(s) 326, 470
BtsIMutI CAGTG 5 cut(s) 211, 246, 326, 470, 943
Cac8I GCNNGC 5 cut(s) 381, 1282, 1341, 1403, 1473
CaiI CAGNNNCTG 2 cut(s) 1349, 1424
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 1429
Cfr13I GGNCC 1 cut(s) 598
CseI GACGC 1 cut(s) 1370
Csp6I GTAC 3 cut(s) 252, 987, 1314
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 4 cut(s) 196, 1252, 1454, 1472
CviQI GTAC 3 cut(s) 252, 987, 1314
DdeI CTNAG 7 cut(s) 318, 719, 1018, 1089, 1217, 1359, 1467
DpnI GATC 2 cut(s) 123, 724
DpnII GATC 2 cut(s) 121, 722
DraI TTTAAA 3 cut(s) 153, 366, 685
Eam1104I CTCTTC 4 cut(s) 38, 656, 995, 1000
EarI CTCTTC 4 cut(s) 38, 656, 995, 1000
Ecl136II GAGCTC 1 cut(s) 1017
Eco24I GRGCYC 2 cut(s) 1019, 1149
Eco47I GGWCC 1 cut(s) 598
Eco53kI GAGCTC 1 cut(s) 1017
Eco88I CYCGRG 1 cut(s) 1203
EcoICRI GAGCTC 1 cut(s) 1017
EcoO109I RGGNCCY 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 713
EcoT38I GRGCYC 2 cut(s) 1019, 1149
FaeI CATG 4 cut(s) 199, 1255, 1457, 1475
FalI AAGNNNNNCTT 2 cut(s) 176, 208
FatI CATG 4 cut(s) 195, 1251, 1453, 1471
FauNDI CATATG 1 cut(s) 523
Fnu4HI GCNGC 2 cut(s) 1069, 1425
FokI GGATG 6 cut(s) 37, 150, 518, 702, 962, 1063
FriOI GRGCYC 2 cut(s) 1019, 1149
Fsp4HI GCNGC 2 cut(s) 1069, 1425
FspBI CTAG 6 cut(s) 407, 624, 1053, 1124, 1340, 1406
GlaI GCGC 1 cut(s) 1428
GluI GCNGC 2 cut(s) 1069, 1425
GsuI CTGGAG 1 cut(s) 920
HapII CCGG 2 cut(s) 167, 757
HgaI GACGC 1 cut(s) 1370
HhaI GCGC 1 cut(s) 1429
Hin1II CATG 4 cut(s) 199, 1255, 1457, 1475
Hin6I GCGC 1 cut(s) 1427
HinP1I GCGC 1 cut(s) 1427
HincII GTYRAC 1 cut(s) 1385
HindII GTYRAC 1 cut(s) 1385
HindIII AAGCTT 3 cut(s) 259, 368, 1100
HinfI GANTC 2 cut(s) 83, 563
HpaII CCGG 2 cut(s) 167, 757
HphI GGTGA 2 cut(s) 449, 1220
Hpy166II GTNNAC 3 cut(s) 324, 1108, 1385
Hpy188I TCNGA 7 cut(s) 88, 319, 613, 642, 1021, 1159, 1218
Hpy8I GTNNAC 3 cut(s) 324, 1108, 1385
HpyCH4III ACNGT 6 cut(s) 404, 617, 649, 925, 938, 1420
HpyCH4IV ACGT 1 cut(s) 1511
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 267, 376
HpyF3I CTNAG 7 cut(s) 318, 719, 1018, 1089, 1217, 1359, 1467
HpySE526I ACGT 1 cut(s) 1511
Hsp92II CATG 4 cut(s) 199, 1255, 1457, 1475
HspAI GCGC 1 cut(s) 1427
Kzo9I GATC 2 cut(s) 121, 722
LmnI GCTCC 3 cut(s) 1119, 1144, 1277
Lsp1109I GCAGC 2 cut(s) 1055, 1411
LweI GCATC 1 cut(s) 1085
MaeI CTAG 6 cut(s) 407, 624, 1053, 1124, 1340, 1406
MaeII ACGT 1 cut(s) 1511
MaeIII GTNAC 3 cut(s) 584, 1226, 1514
MalI GATC 2 cut(s) 123, 724
MboI GATC 2 cut(s) 121, 722
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 2 cut(s) 1019, 1149
MluCI AATT 8 cut(s) 283, 474, 544, 680, 713, 884, 918, 1290
MroXI GAANNNNTTC 1 cut(s) 191
MseI TTAA 6 cut(s) 152, 365, 459, 533, 684, 1245
MslI CAYNNNNRTG 1 cut(s) 292
MspI CCGG 2 cut(s) 167, 757
MspR9I CCNGG 1 cut(s) 757
Mva1269I GAATGC 1 cut(s) 229
MwoI GCNNNNNNNGC 3 cut(s) 105, 267, 376
NciI CCSGG 1 cut(s) 757
NdeI CATATG 1 cut(s) 523
NdeII GATC 2 cut(s) 121, 722
NheI GCTAGC 1 cut(s) 1339
NlaIII CATG 4 cut(s) 199, 1255, 1457, 1475
NlaIV GGNNCC 3 cut(s) 697, 1121, 1146
NmuCI GTSAC 2 cut(s) 584, 1226
NspI RCATGY 1 cut(s) 1475
NspV TTCGAA 1 cut(s) 1287
PaeI GCATGC 1 cut(s) 1475
PaeR7I CTCGAG 1 cut(s) 1203
PagI TCATGA 1 cut(s) 195
PctI GAATGC 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 2 cut(s) 83, 563
PkrI GCNGC 2 cut(s) 1070, 1426
Ppu21I YACGTR 1 cut(s) 1512
PpuMI RGGWCCY 1 cut(s) 598
PsiI TTATAA 1 cut(s) 549
Psp124BI GAGCTC 1 cut(s) 1019
Psp5II RGGWCCY 1 cut(s) 598
PspN4I GGNNCC 3 cut(s) 697, 1121, 1146
PspPI GGNCC 1 cut(s) 598
PspPPI RGGWCCY 1 cut(s) 598
PstNI CAGNNNCTG 2 cut(s) 1349, 1424
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 3 cut(s) 253, 988, 1315
RsaNI GTAC 3 cut(s) 252, 987, 1314
RseI CAYNNNNRTG 1 cut(s) 292
SacI GAGCTC 1 cut(s) 1019
SaqAI TTAA 6 cut(s) 152, 365, 459, 533, 684, 1245
SatI GCNGC 2 cut(s) 1069, 1425
Sau3AI GATC 2 cut(s) 121, 722
Sau96I GGNCC 1 cut(s) 598
ScaI AGTACT 1 cut(s) 253
ScrFI CCNGG 1 cut(s) 757
SduI GDGCHC 2 cut(s) 1019, 1149
SfaNI GCATC 1 cut(s) 1085
SfcI CTRYAG 1 cut(s) 1416
Sfr274I CTCGAG 1 cut(s) 1203
SfuI TTCGAA 1 cut(s) 1287
SinI GGWCC 1 cut(s) 598
SlaI CTCGAG 1 cut(s) 1203
SmiMI CAYNNNNRTG 1 cut(s) 292
SmlI CTYRAG 1 cut(s) 1203
SmoI CTYRAG 1 cut(s) 1203
SpeI ACTAGT 1 cut(s) 623
SphI GCATGC 1 cut(s) 1475
Sse9I AATT 8 cut(s) 283, 474, 544, 680, 713, 884, 918, 1290
SsiI CCGC 1 cut(s) 53
SspI AATATT 1 cut(s) 574
SspMI CTAG 6 cut(s) 407, 624, 1053, 1124, 1340, 1406
SstI GAGCTC 1 cut(s) 1019
StyD4I CCNGG 1 cut(s) 755
TaaI ACNGT 6 cut(s) 404, 617, 649, 925, 938, 1420
TaiI ACGT 1 cut(s) 1514
TaqI TCGA 2 cut(s) 1204, 1287
TasI AATT 8 cut(s) 283, 474, 544, 680, 713, 884, 918, 1290
TatI WGTACW 2 cut(s) 251, 1313
TfiI GAWTC 2 cut(s) 83, 563
Tru1I TTAA 6 cut(s) 152, 365, 459, 533, 684, 1245
Tru9I TTAA 6 cut(s) 152, 365, 459, 533, 684, 1245
TscAI CASTG 5 cut(s) 218, 246, 333, 470, 943
TseFI GTSAC 2 cut(s) 584, 1226
TseI GCWGC 2 cut(s) 1068, 1424
Tsp45I GTSAC 2 cut(s) 584, 1226
TspDTI ATGAA 8 cut(s) 184, 212, 276, 966, 972, 1053, 1112, 1268
TspGWI ACGGA 1 cut(s) 603
TspRI CASTG 5 cut(s) 218, 246, 333, 470, 943
VpaK11BI GGWCC 1 cut(s) 598
XapI RAATTY 5 cut(s) 283, 474, 680, 713, 1290
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 1 cut(s) 1475
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XhoI CTCGAG 1 cut(s) 1203
XmnI GAANNNNTTC 1 cut(s) 191
XspI CTAG 6 cut(s) 407, 624, 1053, 1124, 1340, 1406
ZrmI AGTACT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.