FvH4_5g35250

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000316
Physical Location & Seq
Reverse (-)
25796754 .. 25803506
6753 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35250.t4

Sequence Viewer

Length: 1632 bp
ATGAAGAAGAAGAGTGTGGAGGAGGAAGAAAAGGAAGAGGACTGGTGCTTTTGCTGCTATGATGGTGGAAATCTTATGATCTGCGACCACCAGGGTTGCTTAAAAGTGTATCATCCAGGATGCGTGGGAAAAAAGAAAAACTCCATGCACTCTGGAAAGCGCTGGACTTGCAGTAGGCATTCTTGTGTAGTATGCTCTGGTACTTCAATGTTTTATTGCCTTTGTTGTCCACATGCGTTATGTAGAGATTGTGTGGATGCTTCTGAACTCTCACTACTAAGTGGGAAGAATGGCTTGTGCAAGAGCTGTGTAAAACGGATTCTGCTAGCAGAACAAAACTCAGAGAAAATAGACTTGGAGGATCAAGATGAATGGCTATTTAAAGAATATTGGGAGATTGTCAAGGAAAAAGAAGGATTGACTTCAGGTGATGTTTATTCAGCAAATGCCGATTTGAAGAAGAGAGAAAATCAAAGTTCCAGAGTTTCGCCAGTAATTATTAGTGACAGTGATGAAAGTAAGGACTTAATGTCAGATAGCGACACTAGTGGCACTGGTGTTACAGAAATTCCAAAGCCAATGACAATGAGGAAGGCATCTAAGCGGCTGGTGTTTATAGGATGGGGTTCTGAACCTCTCATCAAGTTCCTAAAATCCATTGGGAAGGATACAACCGAAAAGTTATCTCAGCTTGATTTGGATAAAATTATCTACGACTACATCAGAGATAATGAGCTTTATGACACCAAAAACAAGAGAAAAGCTCTATGTGATCAAAGTCTGCAATCCATTTTCAGAACAAAATCTGTACTCTTACAAAAGATATATGGTCTTCTATGGGAACATCTTGCTGAGAACGTAACACAGGATAGCGAAAGCGAGGATGAAGATGAAATCAAATTGTTAGATAAGAACAAAGGTACTGCACATGACCACAAGAAGAGAAAAGTGAGCTCTGATACAACCATAGAATCTGATGAACAGAAAGCGAGTCATAGATTCCAAAAGAGCTGTTTCGCAGCTATTGTTCCTGAGAATATGAAGCTTGTTTACATAAAGAAGAGCTTATTGGAGAAGTTACTGAAGGAGCCTGACCATTCGGAAAGCAAGATACTGGGGAGTTTTGTAAGAGTGAAAAATGATCCCCTAGACTACCTACAGAGAAGTACTCACCAACTTTCACAAGTTAAAGGCATAAAGGAAATCTTAGCAACCAATGGTGAGGGCAATAAAATTCTACTGCAAGTTTCCTATTTTGTAAAAGATATTCCCCTTTCTCTGCTTTCAGATTCAGACTTCAGTCAGGAAGAGTGTGAAGATTTGTGCCAAAACATGGCAAATGGCCGTCTAAAGAAACCTACTGTTGTTGAGCTCGAAAAGAAGGCAAGAGAACTACATCAGGACATAACTAAGCATTGGATTGAAAAGCAGTTGGTGAGATTGCAGAATTGGGTTGATCGGGCAAATGAGAAAGGATGGAGAACAGAGCTATATGAATATCTTGACCAGAGAGAGCTACTAAAGAAACCATCTGAACAGGAGCGGCTATTAAAGCAGATGCCAGAAGTCATTGCTGAGATTACAGATCTTGAATCTGGTTTTTCAGATTCATTGGTGATGAACAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plus-3 SWIB

Protein Analysis

544

Amino Acids

62.29

Weight (kDa)

6.01

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 210 - 283 2.5e-11 SWIB/MDM2 domain
SWIB_eIF2D PF26291 213 - 288 9e-06 eIF2D, SWIB domain
Plus-3 PF03126 346 - 445 2.7e-14 Plus-3 domain
NERD_plant PF25980 467 - 516 3.4e-23 Plant NERD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G33300
fragaria_vesca FvH4_3g26472 FvH4_5g30650 FvH4_5g30650 FvH4_5g30650 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250
malus_domestica MD08G1198100.v1.1 MD08G1222000.v1.1 MD15G1385400.v1.1 MD15G1440800.v1.1
prunus_persica Prupe.1G532100_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1
pyrus_communis pycom08g17030 pycom08g17040 pycom08g19270 pycom08g19280 pycom15g34550 pycom15g36860 pycom15g38860
rosa_chinensis RchiOBHm_Chr6g0258571 RchiOBHm_Chr6g0258581 RchiOBHm_Chr6g0258621 RchiOBHm_Chr7g0225511 RchiOBHm_Chr7g0230561 RchiOBHm_Chr7g0230571 RchiOBHm_Chr7g0236371 RchiOBHm_Chr7g0236391
rosa_laevigata RLG00000001080 RLG00000001810 RLG00000014605
rosa_multiflora Rmu_co8399019.1_g000001 Rmu_sc0002222.1_g000016 Rmu_sc0002449.1_g000032 Rmu_sc0003720.1_g000005 Rmu_sc0005578.1_g000003 Rmu_sc0011035.1_g000002 Rmu_sc0012681.1_g000001 Rmu_sc0023362.1_g000001 Rmu_sc0023858.1_g000001 Rmu_sc0029314.1_g000001
rosa_roxburghii Rroxscaffold_178G00437500 Rroxscaffold_178G00437540 Rroxscaffold_3G00225100 Rroxscaffold_3G00225110 Rroxscaffold_3G00225140 Rroxscaffold_3G00225170 Rroxscaffold_3G00225190 Rroxscaffold_3G00229880 Rroxscaffold_3G00233730 Rroxscaffold_7G00205760 Rroxscaffold_7G00205780 Rroxscaffold_7G00205820 Rroxscaffold_7G00205860
rosa_rugosa Rorug03G0221800 Rorug03G0270500 Rorug05G0313300 Rorug05G0582400 Rorug05G0582500 Rorug07G0230200.1 Rorug07G0230300 Rorug07G0230400 Rorug07G0295100 Rorug07G0296900
rosa_samantha Rh6AG098800 Rh6AG098900 Rh6AG184000 Rh6BG090700 Rh6BG090900 Rh6CG087500 Rh6CG087600 Rh6DG081800 Rh6DG082000 Rh7AG373100 Rh7AG412900 Rh7AG413000 Rh7AG451100 Rh7AG451200 Rh7BG364900 Rh7BG422800 Rh7BG422900 Rh7CG391400 Rh7CG431300 Rh7CG431400 Rh7CG469300 Rh7CG470800 Rh7CG471300 Rh7CG471400 Rh7DG367900 Rh7DG368000 Rh7DG375800 Rh7DG408800 Rh7DG440500 Rh7DG440600
rosa_wichuraiana Rw0G007810 Rw6G008570 Rw6G008580 Rw7G031720 Rw7G034090 Rw7G037500 Rw7G037510 Rw7G037580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1333
AccBSI CCGCTC 1 cut(s) 1543
AciI CCGC 2 cut(s) 604, 1543
AclWI GGATC 2 cut(s) 369, 1136
AcoI YGGCCR 1 cut(s) 1342
AcsI RAATTY 2 cut(s) 567, 1233
AcuI CTGAAG 3 cut(s) 408, 1103, 1282
AfaI GTAC 4 cut(s) 202, 810, 922, 1168
AfeI AGCGCT 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 1333
AgsI TTSAA 4 cut(s) 207, 457, 1424, 1592
AhdI GACNNNNNGTC 1 cut(s) 529
AhlI ACTAGT 1 cut(s) 545
AjnI CCWGG 2 cut(s) 90, 115
AjuI GAANNNNNNNTTGG 4 cut(s) 338, 370, 1052, 1084
Alw21I GWGCWC 2 cut(s) 956, 1374
AlwI GGATC 2 cut(s) 369, 1136
Aor51HI AGCGCT 1 cut(s) 161
AoxI GGCC 1 cut(s) 1342
ApeKI GCWGC 2 cut(s) 54, 1019
ApoI RAATTY 2 cut(s) 567, 1233
AspLEI GCGC 1 cut(s) 162
AsuHPI GGTGA 5 cut(s) 440, 1163, 1232, 1447, 1627
AsuNHI GCTAGC 1 cut(s) 325
BanII GRGCYC 2 cut(s) 956, 1374
BarI GAAGNNNNNNTAC 2 cut(s) 1034, 1066
BbsI GAAGAC 1 cut(s) 824
Bbv12I GWGCWC 2 cut(s) 956, 1374
BbvI GCAGC 2 cut(s) 41, 1031
BccI CCATC 4 cut(s) 56, 615, 1470, 1537
BceAI ACGGC 1 cut(s) 1329
BciT130I CCWGG 2 cut(s) 92, 117
BciVI GTATCC 1 cut(s) 661
BclI TGATCA 1 cut(s) 772
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 3 cut(s) 326, 546, 1148
BfmI CTRYAG 1 cut(s) 1157
BfoI RGCGCY 1 cut(s) 163
BfuI GTATCC 1 cut(s) 661
BglII AGATCT 1 cut(s) 1585
BisI GCNGC 4 cut(s) 55, 605, 1020, 1544
BlsI GCNGC 4 cut(s) 56, 606, 1021, 1545
BmcAI AGTACT 1 cut(s) 1168
Bme1390I CCNGG 2 cut(s) 92, 117
BmeRI GACNNNNNGTC 1 cut(s) 529
BmiI GGNNCC 1 cut(s) 1089
BmrFI CCNGG 2 cut(s) 92, 117
BmrI ACTGGG 1 cut(s) 1124
BmsI GCATC 4 cut(s) 110, 247, 605, 1548
BmtI GCTAGC 1 cut(s) 329
BmuI ACTGGG 1 cut(s) 1124
BoxI GACNNNNGTC 1 cut(s) 1299
BpiI GAAGAC 1 cut(s) 824
BplI GAGNNNNNCTC 4 cut(s) 748, 780, 1153, 1185
BsaJI CCNNGG 1 cut(s) 91
Bsc4I CCNNNNNNNGG 1 cut(s) 1333
Bse1I ACTGG 4 cut(s) 47, 491, 559, 1119
Bse3DI GCAATG 1 cut(s) 1569
BseBI CCWGG 2 cut(s) 92, 117
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 6 cut(s) 112, 125, 262, 626, 889, 1481
BseLI CCNNNNNNNGG 1 cut(s) 1333
BseMI GCAATG 1 cut(s) 1569
BseMII CTCAG 5 cut(s) 354, 701, 843, 1023, 1566
BseNI ACTGG 4 cut(s) 47, 491, 559, 1119
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 2 cut(s) 41, 1031
BsgI GTGCAG 1 cut(s) 909
BshFI GGCC 1 cut(s) 1344
BsiHKAI GWGCWC 2 cut(s) 956, 1374
BslI CCNNNNNNNGG 1 cut(s) 1333
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 1 cut(s) 1344
Bsp1286I GDGCHC 2 cut(s) 956, 1374
Bsp143I GATC 6 cut(s) 78, 361, 772, 1141, 1456, 1585
BspACI CCGC 2 cut(s) 604, 1543
BspANI GGCC 1 cut(s) 1344
BspCNI CTCAG 5 cut(s) 353, 700, 844, 1024, 1567
BspLI GGNNCC 1 cut(s) 1089
BspOI GCTAGC 1 cut(s) 329
BspPI GGATC 2 cut(s) 369, 1136
BspQI GCTCTTC 1 cut(s) 1055
BsrBI CCGCTC 1 cut(s) 1543
BsrDI GCAATG 1 cut(s) 1569
BsrI ACTGG 4 cut(s) 47, 491, 559, 1119
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 6 cut(s) 78, 361, 772, 1141, 1456, 1585
Bst2UI CCWGG 2 cut(s) 92, 117
Bst4CI ACNGT 2 cut(s) 509, 1363
Bst6I CTCTTC 6 cut(s) 5, 30, 455, 935, 1055, 1302
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 9 cut(s) 278, 340, 600, 687, 852, 1032, 1207, 1410, 1575
BstF5I GGATG 6 cut(s) 112, 125, 262, 626, 889, 1481
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 6 cut(s) 81, 364, 775, 1144, 1459, 1588
BstMBI GATC 6 cut(s) 78, 361, 772, 1141, 1456, 1585
BstMWI GCNNNNNNNGC 3 cut(s) 54, 168, 1552
BstNI CCWGG 2 cut(s) 92, 117
BstNSI RCATGY 1 cut(s) 236
BstPAI GACNNNNGTC 1 cut(s) 1299
BstSCI CCNGG 2 cut(s) 90, 115
BstSFI CTRYAG 1 cut(s) 1157
BstV1I GCAGC 2 cut(s) 41, 1031
BstV2I GAAGAC 1 cut(s) 824
BstX2I RGATCY 1 cut(s) 1585
BstYI RGATCY 1 cut(s) 1585
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 1 cut(s) 1344
BtsCI GGATG 6 cut(s) 112, 125, 262, 626, 889, 1481
BtsIMutI CAGTG 2 cut(s) 514, 552
Cac8I GCNNGC 1 cut(s) 327
CfoI GCGC 1 cut(s) 162
Csp6I GTAC 4 cut(s) 201, 809, 921, 1167
CspCI CAANNNNNGTGG 2 cut(s) 77, 112
CviAII CATG 4 cut(s) 145, 233, 929, 1333
CviQI GTAC 4 cut(s) 201, 809, 921, 1167
DdeI CTNAG 9 cut(s) 278, 340, 600, 687, 852, 1032, 1207, 1410, 1575
DpnI GATC 6 cut(s) 80, 363, 774, 1143, 1458, 1587
DpnII GATC 6 cut(s) 78, 361, 772, 1141, 1456, 1585
DraI TTTAAA 1 cut(s) 382
DriI GACNNNNNGTC 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 1342
Eam1104I CTCTTC 6 cut(s) 5, 30, 455, 935, 1055, 1302
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 6 cut(s) 5, 30, 455, 935, 1055, 1302
Ecl136II GAGCTC 2 cut(s) 954, 1372
Eco24I GRGCYC 2 cut(s) 956, 1374
Eco47III AGCGCT 1 cut(s) 161
Eco53kI GAGCTC 2 cut(s) 954, 1372
Eco57I CTGAAG 3 cut(s) 408, 1103, 1282
EcoICRI GAGCTC 2 cut(s) 954, 1372
EcoRII CCWGG 2 cut(s) 90, 115
EcoT38I GRGCYC 2 cut(s) 956, 1374
FaeI CATG 4 cut(s) 148, 236, 932, 1336
FalI AAGNNNNNCTT 6 cut(s) 278, 310, 1049, 1081, 1190, 1222
FatI CATG 4 cut(s) 144, 232, 928, 1332
FbaI TGATCA 1 cut(s) 772
Fnu4HI GCNGC 4 cut(s) 55, 605, 1020, 1544
FokI GGATG 6 cut(s) 99, 132, 269, 633, 896, 1488
FriOI GRGCYC 2 cut(s) 956, 1374
Fsp4HI GCNGC 4 cut(s) 55, 605, 1020, 1544
FspBI CTAG 3 cut(s) 326, 546, 1148
GlaI GCGC 1 cut(s) 161
GluI GCNGC 4 cut(s) 55, 605, 1020, 1544
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 1 cut(s) 1344
HhaI GCGC 1 cut(s) 162
Hin1II CATG 4 cut(s) 148, 236, 932, 1336
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 1043
HinfI GANTC 7 cut(s) 319, 971, 991, 999, 1289, 1592, 1607
HphI GGTGA 5 cut(s) 440, 1163, 1232, 1447, 1627
Hpy166II GTNNAC 2 cut(s) 230, 1051
Hpy188III TCNNGA 8 cut(s) 153, 365, 480, 1031, 1304, 1400, 1502, 1589
Hpy8I GTNNAC 2 cut(s) 230, 1051
HpyAV CCTTC 5 cut(s) 407, 586, 658, 1078, 1375
HpyCH4III ACNGT 2 cut(s) 509, 1363
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 7 cut(s) 148, 171, 300, 784, 926, 1243, 1444
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 168, 1552
HpyF3I CTNAG 9 cut(s) 278, 340, 600, 687, 852, 1032, 1207, 1410, 1575
HpySE526I ACGT 1 cut(s) 858
Hsp92II CATG 4 cut(s) 148, 236, 932, 1336
HspAI GCGC 1 cut(s) 160
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 6 cut(s) 78, 361, 772, 1141, 1456, 1585
LguI GCTCTTC 1 cut(s) 1055
LmnI GCTCC 2 cut(s) 1087, 1540
Lsp1109I GCAGC 2 cut(s) 41, 1031
LweI GCATC 4 cut(s) 110, 247, 605, 1548
MaeI CTAG 3 cut(s) 326, 546, 1148
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 4 cut(s) 503, 559, 859, 1077
MalI GATC 6 cut(s) 80, 363, 774, 1143, 1458, 1587
MbiI CCGCTC 1 cut(s) 1543
MboI GATC 6 cut(s) 78, 361, 772, 1141, 1456, 1585
MflI RGATCY 1 cut(s) 1585
MhlI GDGCHC 2 cut(s) 956, 1374
MluCI AATT 6 cut(s) 495, 567, 705, 899, 1233, 1447
MlyI GAGTC 1 cut(s) 1000
MnlI CCTC 8 cut(s) 13, 16, 31, 352, 582, 645, 874, 1216
MseI TTAA 5 cut(s) 101, 381, 527, 1188, 1550
MslI CAYNNNNRTG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 92, 117
Mva1269I GAATGC 1 cut(s) 178
MvaI CCWGG 2 cut(s) 92, 117
MwoI GCNNNNNNNGC 3 cut(s) 54, 168, 1552
NdeII GATC 6 cut(s) 78, 361, 772, 1141, 1456, 1585
NheI GCTAGC 1 cut(s) 325
NlaIII CATG 4 cut(s) 148, 236, 932, 1336
NlaIV GGNNCC 1 cut(s) 1089
NmuCI GTSAC 1 cut(s) 503
NspI RCATGY 1 cut(s) 236
PciSI GCTCTTC 1 cut(s) 1055
PctI GAATGC 1 cut(s) 178
PfeI GAWTC 6 cut(s) 319, 971, 999, 1289, 1592, 1607
PflMI CCANNNNNTGG 1 cut(s) 1333
PfoI TCCNGGA 1 cut(s) 115
PkrI GCNGC 4 cut(s) 56, 606, 1021, 1545
PleI GAGTC 1 cut(s) 999
PpsI GAGTC 1 cut(s) 999
PshAI GACNNNNGTC 1 cut(s) 1299
Psp124BI GAGCTC 2 cut(s) 956, 1374
Psp6I CCWGG 2 cut(s) 90, 115
PspGI CCWGG 2 cut(s) 90, 115
PspN4I GGNNCC 1 cut(s) 1089
PsrI GAACNNNNNNTAC 2 cut(s) 258, 290
PsuI RGATCY 1 cut(s) 1585
RsaI GTAC 4 cut(s) 202, 810, 922, 1168
RsaNI GTAC 4 cut(s) 201, 809, 921, 1167
RseI CAYNNNNRTG 1 cut(s) 183
SacI GAGCTC 2 cut(s) 956, 1374
SapI GCTCTTC 1 cut(s) 1055
SaqAI TTAA 5 cut(s) 101, 381, 527, 1188, 1550
SatI GCNGC 4 cut(s) 55, 605, 1020, 1544
Sau3AI GATC 6 cut(s) 78, 361, 772, 1141, 1456, 1585
ScaI AGTACT 1 cut(s) 1168
SchI GAGTC 1 cut(s) 1000
ScrFI CCNGG 2 cut(s) 92, 117
SduI GDGCHC 2 cut(s) 956, 1374
SfaNI GCATC 4 cut(s) 110, 247, 605, 1548
SfcI CTRYAG 1 cut(s) 1157
SmiMI CAYNNNNRTG 1 cut(s) 183
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 6 cut(s) 495, 567, 705, 899, 1233, 1447
SsiI CCGC 2 cut(s) 604, 1543
SspI AATATT 1 cut(s) 389
SspMI CTAG 3 cut(s) 326, 546, 1148
SstI GAGCTC 2 cut(s) 956, 1374
StyD4I CCNGG 2 cut(s) 90, 115
TaaI ACNGT 2 cut(s) 509, 1363
TaiI ACGT 1 cut(s) 861
TaqI TCGA 1 cut(s) 1374
TasI AATT 6 cut(s) 495, 567, 705, 899, 1233, 1447
TatI WGTACW 2 cut(s) 808, 1166
TauI GCSGC 2 cut(s) 607, 1546
TfiI GAWTC 6 cut(s) 319, 971, 999, 1289, 1592, 1607
Tru1I TTAA 5 cut(s) 101, 381, 527, 1188, 1550
Tru9I TTAA 5 cut(s) 101, 381, 527, 1188, 1550
TscAI CASTG 2 cut(s) 514, 559
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 2 cut(s) 54, 1019
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 9 cut(s) 17, 384, 528, 900, 906, 993, 1055, 1509, 1599
TspGWI ACGGA 1 cut(s) 331
TspRI CASTG 2 cut(s) 514, 559
Van91I CCANNNNNTGG 1 cut(s) 1333
XapI RAATTY 2 cut(s) 567, 1233
XceI RCATGY 1 cut(s) 236
XspI CTAG 3 cut(s) 326, 546, 1148
ZrmI AGTACT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.