RchiOBHm_Chr7g0230561

Plus-3 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000316
Physical Location & Seq
Reverse (-)
54129085 .. 54129816
732 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20660

Sequence Viewer

Length: 714 bp
ATGTCAGACAGCGACACTAGTGGCACTGGTGTTACAGAAATTCAGAAGCCAATGAGATTAAGAAAGGGATCTAAGCGACTAGAGTTTATAGGATGGGGTTCTAAACCTCTCATTGAGTTTCTAAGATCCATTGGGAAGGATACAACTGAGAAGATATCCCAGCTTGATTTGGACAAGATTATCTGTAACTACATCAGAGATAAAGAGCTTTATGTGACCAAGGACAGGAGAAAGGCTCTATGTGATCAAAGTCTGCAATCCATTTTCAGAACGAAATTTGTATTCCTGGTAAGGATATATGGTCTTCTAGGGGAACATCTTGCCGAGAACATAACAGAGGATAGTGGAAGCGAGGATGAGGAAGAAATCAAATTGGAAGATAAGAACAATGGTACTACTCATGAACGCAAGAAGAGAAGAGTGAGCTCTGATACAACCATAGCATCTGATGAAAAGAAAGCAAGTCCTAGATTCCAAAAGGGCTGTTTTGCATCTATTATCCCTGAGAATATGAAGCTTGTTTACGTAAAGAGGAGCTTACTGGAGAAGTTACTGAAGGAGCCTGATCATTCGGAAAGCAAGGTACTAGGGAGTTTTGTGAGAGTGAAAAATAACCCCCTAGACTACCTTCAGAGAAATACTCACCAACTTTCACAAGTTAAAGGTATTGTGTGTACTATGCAGTCAATATCATGTGATGCATTCTGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plus-3 SWIB

Protein Analysis

237

Amino Acids

27.15

Weight (kDa)

8.91

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIB PF02201 34 - 107 1.6e-08 SWIB/MDM2 domain
Plus-3 PF03126 170 - 223 7.7e-08 Plus-3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G33300
fragaria_vesca FvH4_3g26472 FvH4_5g30650 FvH4_5g30650 FvH4_5g30650 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250
malus_domestica MD08G1198100.v1.1 MD08G1222000.v1.1 MD15G1385400.v1.1 MD15G1440800.v1.1
prunus_persica Prupe.1G532100_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1
pyrus_communis pycom08g17030 pycom08g17040 pycom08g19270 pycom08g19280 pycom15g34550 pycom15g36860 pycom15g38860
rosa_chinensis RchiOBHm_Chr6g0258571 RchiOBHm_Chr6g0258581 RchiOBHm_Chr6g0258621 RchiOBHm_Chr7g0225511 RchiOBHm_Chr7g0230561 RchiOBHm_Chr7g0230571 RchiOBHm_Chr7g0236371 RchiOBHm_Chr7g0236391
rosa_laevigata RLG00000001080 RLG00000001810 RLG00000014605
rosa_multiflora Rmu_co8399019.1_g000001 Rmu_sc0002222.1_g000016 Rmu_sc0002449.1_g000032 Rmu_sc0003720.1_g000005 Rmu_sc0005578.1_g000003 Rmu_sc0011035.1_g000002 Rmu_sc0012681.1_g000001 Rmu_sc0023362.1_g000001 Rmu_sc0023858.1_g000001 Rmu_sc0029314.1_g000001
rosa_roxburghii Rroxscaffold_178G00437500 Rroxscaffold_178G00437540 Rroxscaffold_3G00225100 Rroxscaffold_3G00225110 Rroxscaffold_3G00225140 Rroxscaffold_3G00225170 Rroxscaffold_3G00225190 Rroxscaffold_3G00229880 Rroxscaffold_3G00233730 Rroxscaffold_7G00205760 Rroxscaffold_7G00205780 Rroxscaffold_7G00205820 Rroxscaffold_7G00205860
rosa_rugosa Rorug03G0221800 Rorug03G0270500 Rorug05G0313300 Rorug05G0582400 Rorug05G0582500 Rorug07G0230200.1 Rorug07G0230300 Rorug07G0230400 Rorug07G0295100 Rorug07G0296900
rosa_samantha Rh6AG098800 Rh6AG098900 Rh6AG184000 Rh6BG090700 Rh6BG090900 Rh6CG087500 Rh6CG087600 Rh6DG081800 Rh6DG082000 Rh7AG373100 Rh7AG412900 Rh7AG413000 Rh7AG451100 Rh7AG451200 Rh7BG364900 Rh7BG422800 Rh7BG422900 Rh7CG391400 Rh7CG431300 Rh7CG431400 Rh7CG469300 Rh7CG470800 Rh7CG471300 Rh7CG471400 Rh7DG367900 Rh7DG368000 Rh7DG375800 Rh7DG408800 Rh7DG440500 Rh7DG440600
rosa_wichuraiana Rw0G007810 Rw6G008570 Rw6G008580 Rw7G031720 Rw7G034090 Rw7G037500 Rw7G037510 Rw7G037580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 76, 120
AcsI RAATTY 2 cut(s) 39, 275
AcuI CTGAAG 2 cut(s) 575, 614
AfaI GTAC 3 cut(s) 394, 585, 676
AfiI CCNNNNNNNGG 1 cut(s) 225
AhlI ACTAGT 1 cut(s) 17
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 5 cut(s) 163, 208, 426, 517, 537
AluI AGCT 5 cut(s) 163, 208, 426, 517, 537
Alw21I GWGCWC 1 cut(s) 428
AlwI GGATC 2 cut(s) 76, 120
ApoI RAATTY 2 cut(s) 39, 275
AsuHPI GGTGA 1 cut(s) 635
BanII GRGCYC 1 cut(s) 428
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 428
BccI CCATC 1 cut(s) 87
BciT130I CCWGG 1 cut(s) 287
BciVI GTATCC 1 cut(s) 133
BclI TGATCA 2 cut(s) 244, 565
BcuI ACTAGT 1 cut(s) 17
BfaI CTAG 6 cut(s) 18, 80, 308, 468, 587, 620
BfuI GTATCC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 561
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 3 cut(s) 452, 500, 688
BpiI GAAGAC 1 cut(s) 296
BplI GAGNNNNNCTC 4 cut(s) 220, 252, 625, 657
BpmI CTGGAG 1 cut(s) 563
BsaAI YACGTR 1 cut(s) 526
BsaJI CCNNGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 2 cut(s) 31, 546
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 2 cut(s) 98, 361
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMII CTCAG 2 cut(s) 138, 495
BseNI ACTGG 2 cut(s) 31, 546
BseRI GAGGAG 1 cut(s) 547
BseYI CCCAGC 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 428
BslI CCNNNNNNNGG 1 cut(s) 225
BsmI GAATGC 1 cut(s) 701
Bsp1286I GDGCHC 1 cut(s) 428
Bsp143I GATC 4 cut(s) 68, 125, 244, 565
BspCNI CTCAG 2 cut(s) 139, 496
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 1 cut(s) 561
BspPI GGATC 2 cut(s) 76, 120
BsrI ACTGG 2 cut(s) 31, 546
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 4 cut(s) 68, 125, 244, 565
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 287
Bst6I CTCTTC 2 cut(s) 407, 412
BstBAI YACGTR 1 cut(s) 526
BstDEI CTNAG 4 cut(s) 72, 122, 147, 504
BstF5I GGATG 2 cut(s) 98, 361
BstKTI GATC 4 cut(s) 71, 128, 247, 568
BstMBI GATC 4 cut(s) 68, 125, 244, 565
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstSNI TACGTA 1 cut(s) 526
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 2 cut(s) 68, 125
BstYI RGATCY 2 cut(s) 68, 125
BsuI GTATCC 1 cut(s) 133
BtsCI GGATG 2 cut(s) 98, 361
BtsIMutI CAGTG 1 cut(s) 24
CciI TCATGA 1 cut(s) 400
Csp6I GTAC 3 cut(s) 393, 584, 675
CviAII CATG 2 cut(s) 401, 693
CviJI RGCY 9 cut(s) 49, 163, 208, 236, 426, 483, 517, 537, 562
CviKI_1 RGCY 9 cut(s) 49, 163, 208, 236, 426, 483, 517, 537, 562
CviQI GTAC 3 cut(s) 393, 584, 675
DdeI CTNAG 4 cut(s) 72, 122, 147, 504
DpnI GATC 4 cut(s) 70, 127, 246, 567
DpnII GATC 4 cut(s) 68, 125, 244, 565
Eam1104I CTCTTC 2 cut(s) 407, 412
EarI CTCTTC 2 cut(s) 407, 412
Ecl136II GAGCTC 1 cut(s) 426
Eco105I TACGTA 1 cut(s) 526
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 428
Eco32I GATATC 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 426
Eco57I CTGAAG 2 cut(s) 575, 614
EcoICRI GAGCTC 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 285
EcoRV GATATC 1 cut(s) 156
EcoT14I CCWWGG 1 cut(s) 219
EcoT22I ATGCAT 1 cut(s) 703
EcoT38I GRGCYC 1 cut(s) 428
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 2 cut(s) 404, 696
FalI AAGNNNNNCTT 2 cut(s) 521, 553
FatI CATG 2 cut(s) 400, 692
FbaI TGATCA 2 cut(s) 244, 565
FokI GGATG 2 cut(s) 105, 368
FriOI GRGCYC 1 cut(s) 428
FspBI CTAG 6 cut(s) 18, 80, 308, 468, 587, 620
GsaI CCCAGC 1 cut(s) 163
GsuI CTGGAG 1 cut(s) 563
Hin1II CATG 2 cut(s) 404, 696
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 1 cut(s) 471
HphI GGTGA 1 cut(s) 635
Hpy166II GTNNAC 2 cut(s) 523, 675
Hpy188I TCNGA 8 cut(s) 7, 45, 197, 269, 430, 448, 574, 633
Hpy188III TCNNGA 1 cut(s) 401
Hpy8I GTNNAC 2 cut(s) 523, 675
HpyAV CCTTC 3 cut(s) 130, 550, 638
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 4 cut(s) 256, 491, 682, 701
HpyF3I CTNAG 4 cut(s) 72, 122, 147, 504
HpySE526I ACGT 1 cut(s) 525
Hsp92II CATG 2 cut(s) 404, 696
Ksp22I TGATCA 2 cut(s) 244, 565
Kzo9I GATC 4 cut(s) 68, 125, 244, 565
LmnI GCTCC 2 cut(s) 534, 559
LpnPI CCDG 8 cut(s) 12, 173, 211, 272, 299, 516, 527, 576
LweI GCATC 3 cut(s) 452, 500, 688
MaeI CTAG 6 cut(s) 18, 80, 308, 468, 587, 620
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 5 cut(s) 31, 185, 214, 549, 707
MalI GATC 4 cut(s) 70, 127, 246, 567
MboI GATC 4 cut(s) 68, 125, 244, 565
MboII GAAGA 6 cut(s) 163, 296, 374, 389, 424, 429
MflI RGATCY 2 cut(s) 68, 125
MhlI GDGCHC 1 cut(s) 428
MluCI AATT 3 cut(s) 39, 275, 371
MnlI CCTC 5 cut(s) 117, 331, 346, 352, 525
Mph1103I ATGCAT 1 cut(s) 703
MseI TTAA 2 cut(s) 59, 660
MspR9I CCNGG 1 cut(s) 287
Mva1269I GAATGC 1 cut(s) 701
MvaI CCWGG 1 cut(s) 287
NdeII GATC 4 cut(s) 68, 125, 244, 565
NlaIII CATG 2 cut(s) 404, 696
NlaIV GGNNCC 1 cut(s) 561
NmeAIII GCCGAG 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 703
PagI TCATGA 1 cut(s) 400
PctI GAATGC 1 cut(s) 701
PfeI GAWTC 1 cut(s) 471
Ppu21I YACGTR 1 cut(s) 526
Psp124BI GAGCTC 1 cut(s) 428
Psp6I CCWGG 1 cut(s) 285
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 1 cut(s) 561
PsuI RGATCY 2 cut(s) 68, 125
RsaI GTAC 3 cut(s) 394, 585, 676
RsaNI GTAC 3 cut(s) 393, 584, 675
SacI GAGCTC 1 cut(s) 428
SaqAI TTAA 2 cut(s) 59, 660
Sau3AI GATC 4 cut(s) 68, 125, 244, 565
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 428
SfaNI GCATC 3 cut(s) 452, 500, 688
SnaBI TACGTA 1 cut(s) 526
SpeI ACTAGT 1 cut(s) 17
Sse9I AATT 3 cut(s) 39, 275, 371
SspMI CTAG 6 cut(s) 18, 80, 308, 468, 587, 620
SstI GAGCTC 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 285
StyI CCWWGG 1 cut(s) 219
TaiI ACGT 1 cut(s) 528
TasI AATT 3 cut(s) 39, 275, 371
TatI WGTACW 1 cut(s) 674
TfiI GAWTC 1 cut(s) 471
Tru1I TTAA 2 cut(s) 59, 660
Tru9I TTAA 2 cut(s) 59, 660
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 1 cut(s) 214
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 3 cut(s) 417, 465, 527
TspRI CASTG 1 cut(s) 31
XapI RAATTY 2 cut(s) 39, 275
XspI CTAG 6 cut(s) 18, 80, 308, 468, 587, 620
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.