Rh7AG451100

Chromosome-associated kinesin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000316
Physical Location & Seq
Reverse (-)
61706149 .. 61711412
5264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG451100.1

Sequence Viewer

Length: 1464 bp
ATGATGAAGAAGGCCAACCGCCGCTCCAAGCAGACCGCCGCCGCCGACGATTGCGAGAAGCGAGTTCGCGAACTCGAAATCGAAAACAAAGCCTTTCAGAAGGAGGTTGAGGAGCTGAGATACAAGCTTGCAAATGTTTCATCTACTAGTGGTGTAGAGAACAGTGCTCAGAAACTCAGAGAAAACTACCTGCAGAAGTTGACTTTCCTCGAAGATCAGGTTACGGTGTTGACGAGGAAGCTAGATGCTCAATCTCAACTCTCAACCCAAAGAAGAAGAGGGGACGAGTCAGCAAAGCCGTTCCAGTTTGAGATTCAGAGATTGAAGGCTCAGAAGGTTCAAATGCAATGTAAGATGAAACTAGAATCTGTGCAGTTCAGATTGCATAAAGCTTTGTTGGACAAGGAAGTTCTTCAGCTCAAGAAAGAGAGTAGGAAGAATAAACATGAGATGCACAAGCTATTGGCCTCCAATCAAAGACTGAAGACTGTTTTGCGTCGAAAGACTGAAGAAGCATCTGTGGCTACTAAACAGCTGAGACGGCTCTTAGAATCTCGCAAGGCTTTGTTGCTAAAAAGAGGTGGCAAAAATGGGAATAATACAGCAACTCAGCTGGTGCAGGAAATTGACCATGAGGCTGAAGTCACAGAGCAGTTGAATGACCTATGTGCTATATATGAACGTCAAATAGAAGAGATGGTGGAGGAGGCTGAAAAGCTTAAAGATGAAGTAGAGGCACTGCAACAAGAAAAGTCAAGGTGCTCATGCCAGGAGAAAGAGGTTGACAGCTTTGAGAAGGATTTAGATATAACAGACCTGAAGGCTCAAGTAGTCAGCCTCAGTAGTATGGTTGAACAATTAAGATTGCGCAAGGCTGAGCTTGATCATGGGAAGTCACAGGGCATTAGGAGTCAGCATACTGCATCTGTTGGGAGCAGTTACAAGTTAGTGGAGGACATTAGTCCATCTGCATCAGAAAATTCCACAGTTGGAACATCTAAAACAGCAGCTCCAGTTTGCTGCTCATGCACTAAGAATTCTTTGTGCAAGACGATGAAATGCAAATGTCGATCCAAGGGTGGAAGCTGTGGTGCCTCATGTGGCTGTGCAGCATCCAAGTGCAGCAATAGGAAAGCAGTCCCAATCAAGTCGAGTGACTCGCCACTATCAGAGATTGCTAATGGCGTTCTGAATAGTTCAAACACCAGTGAAACAGTGAAGAGTAGCATAGAGGCTTCTGAAGGTGCAATGCTACTTCAGAGTGCACTAGTTCAGAAGCCTGCTGAACCGGAAGGGAACTTTGGAGCAATAAAGAAACCCTTAACTGAAATTGGGAACATATTGGTGAGTACAAATGCTGTAAAACCTGGCCCAAGAAAAAAGGGGAGAAAGCCAGCGATTCAGCTTGTTACTGTAGACCCAATTTCCTCAGTGCCAGTAAATATGGAAGGAAATGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

487

Amino Acids

54.04

Weight (kDa)

9.23

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIF21A_4th PF25764 2 - 143 2.8e-25 KIF21A-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G33300
fragaria_vesca FvH4_3g26472 FvH4_5g30650 FvH4_5g30650 FvH4_5g30650 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35240 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250 FvH4_5g35250
malus_domestica MD08G1198100.v1.1 MD08G1222000.v1.1 MD15G1385400.v1.1 MD15G1440800.v1.1
prunus_persica Prupe.1G532100_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1 Prupe.1G559800_v2.0.a1
pyrus_communis pycom08g17030 pycom08g17040 pycom08g19270 pycom08g19280 pycom15g34550 pycom15g36860 pycom15g38860
rosa_chinensis RchiOBHm_Chr6g0258571 RchiOBHm_Chr6g0258581 RchiOBHm_Chr6g0258621 RchiOBHm_Chr7g0225511 RchiOBHm_Chr7g0230561 RchiOBHm_Chr7g0230571 RchiOBHm_Chr7g0236371 RchiOBHm_Chr7g0236391
rosa_laevigata RLG00000001080 RLG00000001810 RLG00000014605
rosa_multiflora Rmu_co8399019.1_g000001 Rmu_sc0002222.1_g000016 Rmu_sc0002449.1_g000032 Rmu_sc0003720.1_g000005 Rmu_sc0005578.1_g000003 Rmu_sc0011035.1_g000002 Rmu_sc0012681.1_g000001 Rmu_sc0023362.1_g000001 Rmu_sc0023858.1_g000001 Rmu_sc0029314.1_g000001
rosa_roxburghii Rroxscaffold_178G00437500 Rroxscaffold_178G00437540 Rroxscaffold_3G00225100 Rroxscaffold_3G00225110 Rroxscaffold_3G00225140 Rroxscaffold_3G00225170 Rroxscaffold_3G00225190 Rroxscaffold_3G00229880 Rroxscaffold_3G00233730 Rroxscaffold_7G00205760 Rroxscaffold_7G00205780 Rroxscaffold_7G00205820 Rroxscaffold_7G00205860
rosa_rugosa Rorug03G0221800 Rorug03G0270500 Rorug05G0313300 Rorug05G0582400 Rorug05G0582500 Rorug07G0230200.1 Rorug07G0230300 Rorug07G0230400 Rorug07G0295100 Rorug07G0296900
rosa_samantha Rh6AG098800 Rh6AG098900 Rh6AG184000 Rh6BG090700 Rh6BG090900 Rh6CG087500 Rh6CG087600 Rh6DG081800 Rh6DG082000 Rh7AG373100 Rh7AG412900 Rh7AG413000 Rh7AG451100 Rh7AG451200 Rh7BG364900 Rh7BG422800 Rh7BG422900 Rh7CG391400 Rh7CG431300 Rh7CG431400 Rh7CG469300 Rh7CG470800 Rh7CG471300 Rh7CG471400 Rh7DG367900 Rh7DG368000 Rh7DG375800 Rh7DG408800 Rh7DG440500 Rh7DG440600
rosa_wichuraiana Rw0G007810 Rw6G008570 Rw6G008580 Rw7G031720 Rw7G034090 Rw7G037500 Rw7G037510 Rw7G037580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 869
Acc36I ACCTGC 1 cut(s) 198
AccB1I GGYRCC 1 cut(s) 1091
AccBSI CCGCTC 1 cut(s) 24
AccI GTMKAC 1 cut(s) 1416
AccII CGCG 1 cut(s) 69
AciI CCGC 5 cut(s) 19, 22, 36, 39, 42
AclWI GGATC 1 cut(s) 1065
AcsI RAATTY 2 cut(s) 979, 1036
AcuI CTGAAG 7 cut(s) 398, 503, 528, 660, 839, 1241, 1260
AfaI GTAC 1 cut(s) 1351
AgsI TTSAA 5 cut(s) 325, 341, 658, 854, 1200
AhlI ACTAGT 2 cut(s) 146, 1267
AjnI CCWGG 2 cut(s) 768, 1366
AjuI GAANNNNNNNTTGG 2 cut(s) 1284, 1316
Alw21I GWGCWC 3 cut(s) 169, 764, 1267
Alw26I GTCTC 1 cut(s) 532
Alw44I GTGCAC 1 cut(s) 1263
AlwI GGATC 1 cut(s) 1065
AoxI GGCC 3 cut(s) 12, 465, 1369
ApaLI GTGCAC 1 cut(s) 1263
ApeKI GCWGC 4 cut(s) 1007, 1020, 1109, 1122
ApoI RAATTY 2 cut(s) 979, 1036
Asp700I GAANNNNTTC 1 cut(s) 411
AspLEI GCGC 1 cut(s) 870
AspS9I GGNCC 1 cut(s) 1370
AsuHPI GGTGA 1 cut(s) 1357
BaeGI GKGCMC 1 cut(s) 1267
BanI GGYRCC 1 cut(s) 1091
BbsI GAAGAC 1 cut(s) 491
Bbv12I GWGCWC 3 cut(s) 169, 764, 1267
BbvI GCAGC 4 cut(s) 1007, 1019, 1121, 1134
BccI CCATC 2 cut(s) 691, 973
BceAI ACGGC 2 cut(s) 283, 557
BciT130I CCWGG 2 cut(s) 770, 1368
BclI TGATCA 1 cut(s) 883
BcoDI GTCTC 1 cut(s) 532
BcuI ACTAGT 2 cut(s) 146, 1267
BfaI CTAG 4 cut(s) 147, 242, 362, 1268
BfmI CTRYAG 2 cut(s) 191, 1413
BfuAI ACCTGC 1 cut(s) 198
BisI GCNGC 7 cut(s) 22, 39, 42, 1008, 1021, 1110, 1123
BlpI GCTNAGC 1 cut(s) 876
BlsI GCNGC 7 cut(s) 23, 40, 43, 1009, 1022, 1111, 1124
Bme1390I CCNGG 2 cut(s) 770, 1368
BmgT120I GGNCC 1 cut(s) 1370
BmiI GGNNCC 1 cut(s) 1093
BmrFI CCNGG 2 cut(s) 770, 1368
BmsI GCATC 6 cut(s) 235, 441, 524, 932, 980, 1121
BoxI GACNNNNGTC 1 cut(s) 960
BpiI GAAGAC 1 cut(s) 491
BpmI CTGGAG 1 cut(s) 996
Bpu1102I GCTNAGC 1 cut(s) 876
BpuEI CTTGAG 2 cut(s) 404, 810
BsaJI CCNNGG 1 cut(s) 1074
BsaWI WCCGGW 1 cut(s) 1288
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 994, 1024
Bse1I ACTGG 4 cut(s) 304, 1013, 1206, 1436
Bse3DI GCAATG 2 cut(s) 353, 1254
BseBI CCWGG 2 cut(s) 770, 1368
BseDI CCNNGG 1 cut(s) 1074
BseGI GGATG 1 cut(s) 1112
BseMI GCAATG 2 cut(s) 353, 1254
BseMII CTCAG 9 cut(s) 107, 182, 190, 344, 527, 623, 853, 867, 1443
BseNI ACTGG 4 cut(s) 304, 1013, 1206, 1436
BseRI GAGGAG 2 cut(s) 125, 719
BseSI GKGCMC 1 cut(s) 1267
BseXI GCAGC 4 cut(s) 1007, 1019, 1121, 1134
BsgI GTGCAG 4 cut(s) 392, 638, 1128, 1141
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 3 cut(s) 14, 467, 1371
BshNI GGYRCC 1 cut(s) 1091
BsiHKAI GWGCWC 3 cut(s) 169, 764, 1267
BsiSI CCGG 1 cut(s) 1289
BslFI GGGAC 2 cut(s) 296, 1124
BsmAI GTCTC 1 cut(s) 532
BsmBI CGTCTC 1 cut(s) 532
BsmFI GGGAC 2 cut(s) 296, 1124
BsnI GGCC 3 cut(s) 14, 467, 1371
Bsp1286I GDGCHC 3 cut(s) 169, 764, 1267
Bsp143I GATC 3 cut(s) 214, 883, 1070
Bsp1720I GCTNAGC 1 cut(s) 876
Bsp68I TCGCGA 1 cut(s) 69
BspACI CCGC 5 cut(s) 19, 22, 36, 39, 42
BspANI GGCC 3 cut(s) 14, 467, 1371
BspCNI CTCAG 9 cut(s) 108, 181, 189, 343, 528, 622, 852, 868, 1442
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 1093
BspMAI CTGCAG 1 cut(s) 195
BspMI ACCTGC 1 cut(s) 198
BspPI GGATC 1 cut(s) 1065
BspT107I GGYRCC 1 cut(s) 1091
BsrBI CCGCTC 1 cut(s) 24
BsrDI GCAATG 2 cut(s) 353, 1254
BsrI ACTGG 4 cut(s) 304, 1013, 1206, 1436
BssECI CCNNGG 1 cut(s) 1074
BssMI GATC 3 cut(s) 214, 883, 1070
BssT1I CCWWGG 1 cut(s) 1074
Bst2UI CCWGG 2 cut(s) 770, 1368
Bst4CI ACNGT 6 cut(s) 164, 226, 490, 988, 1216, 1414
Bst6I CTCTTC 3 cut(s) 271, 687, 1214
BstC8I GCNNGC 3 cut(s) 129, 1281, 1395
BstF5I GGATG 1 cut(s) 1112
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 1 cut(s) 870
BstKTI GATC 3 cut(s) 217, 886, 1073
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 3 cut(s) 214, 883, 1070
BstMWI GCNNNNNNNGC 3 cut(s) 521, 541, 1026
BstNI CCWGG 2 cut(s) 770, 1368
BstPAI GACNNNNGTC 1 cut(s) 960
BstSCI CCNGG 2 cut(s) 768, 1366
BstSFI CTRYAG 2 cut(s) 191, 1413
BstSLI GKGCMC 1 cut(s) 1267
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 4 cut(s) 1007, 1019, 1121, 1134
BstV2I GAAGAC 1 cut(s) 491
BsuRI GGCC 3 cut(s) 14, 467, 1371
BtsCI GGATG 1 cut(s) 1112
BtsI GCAGTG 1 cut(s) 737
BtsIMutI CAGTG 5 cut(s) 169, 737, 1213, 1221, 1437
BtuMI TCGCGA 1 cut(s) 69
BveI ACCTGC 1 cut(s) 198
Cac8I GCNNGC 3 cut(s) 129, 1281, 1395
CfoI GCGC 1 cut(s) 870
Cfr13I GGNCC 1 cut(s) 1370
CseI GACGC 1 cut(s) 485
Csp6I GTAC 1 cut(s) 1350
CviAII CATG 6 cut(s) 446, 632, 765, 887, 1026, 1098
CviQI GTAC 1 cut(s) 1350
DpnI GATC 3 cut(s) 216, 885, 1072
DpnII GATC 3 cut(s) 214, 883, 1070
Eam1104I CTCTTC 3 cut(s) 271, 687, 1214
EarI CTCTTC 3 cut(s) 271, 687, 1214
Eco130I CCWWGG 1 cut(s) 1074
Eco57I CTGAAG 7 cut(s) 398, 503, 528, 660, 839, 1241, 1260
EcoRI GAATTC 1 cut(s) 1036
EcoRII CCWGG 2 cut(s) 768, 1366
EcoT14I CCWWGG 1 cut(s) 1074
ErhI CCWWGG 1 cut(s) 1074
Esp3I CGTCTC 1 cut(s) 532
FaeI CATG 6 cut(s) 449, 635, 768, 890, 1029, 1101
FalI AAGNNNNNCTT 2 cut(s) 1304, 1336
FaqI GGGAC 2 cut(s) 296, 1124
FatI CATG 6 cut(s) 445, 631, 764, 886, 1025, 1097
FbaI TGATCA 1 cut(s) 883
FblI GTMKAC 1 cut(s) 1416
Fnu4HI GCNGC 7 cut(s) 22, 39, 42, 1008, 1021, 1110, 1123
FokI GGATG 1 cut(s) 1099
Fsp4HI GCNGC 7 cut(s) 22, 39, 42, 1008, 1021, 1110, 1123
FspBI CTAG 4 cut(s) 147, 242, 362, 1268
FspI TGCGCA 1 cut(s) 869
GlaI GCGC 1 cut(s) 869
GluI GCNGC 7 cut(s) 22, 39, 42, 1008, 1021, 1110, 1123
GsuI CTGGAG 1 cut(s) 996
HaeIII GGCC 3 cut(s) 14, 467, 1371
HapII CCGG 1 cut(s) 1289
HgaI GACGC 1 cut(s) 485
HhaI GCGC 1 cut(s) 870
Hin1II CATG 6 cut(s) 449, 635, 768, 890, 1029, 1101
Hin6I GCGC 1 cut(s) 868
HinP1I GCGC 1 cut(s) 868
HincII GTYRAC 3 cut(s) 201, 231, 784
HindII GTYRAC 3 cut(s) 201, 231, 784
HindIII AAGCTT 3 cut(s) 125, 390, 716
HinfI GANTC 7 cut(s) 287, 313, 365, 551, 910, 1157, 1399
HpaII CCGG 1 cut(s) 1289
HphI GGTGA 1 cut(s) 1357
Hpy166II GTNNAC 5 cut(s) 201, 231, 784, 1265, 1417
Hpy188III TCNNGA 2 cut(s) 68, 421
Hpy8I GTNNAC 5 cut(s) 201, 231, 784, 1265, 1417
Hpy99I CGWCG 2 cut(s) 50, 501
HpyAV CCTTC 9 cut(s) 4, 94, 319, 328, 790, 814, 1235, 1286, 1442
HpyCH4III ACNGT 6 cut(s) 164, 226, 490, 988, 1216, 1414
HpyCH4IV ACGT 1 cut(s) 682
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 541, 1026
HpySE526I ACGT 1 cut(s) 682
Hsp92II CATG 6 cut(s) 449, 635, 768, 890, 1029, 1101
HspAI GCGC 1 cut(s) 868
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 3 cut(s) 214, 883, 1070
LmnI GCTCC 5 cut(s) 29, 112, 933, 1015, 1304
Lsp1109I GCAGC 4 cut(s) 1007, 1019, 1121, 1134
LweI GCATC 6 cut(s) 235, 441, 524, 932, 980, 1121
MaeI CTAG 4 cut(s) 147, 242, 362, 1268
MaeII ACGT 1 cut(s) 682
MaeIII GTNAC 6 cut(s) 220, 643, 894, 938, 1154, 1408
MalI GATC 3 cut(s) 216, 885, 1072
MbiI CCGCTC 1 cut(s) 24
MboI GATC 3 cut(s) 214, 883, 1070
MhlI GDGCHC 3 cut(s) 169, 764, 1267
MluCI AATT 6 cut(s) 624, 857, 979, 1036, 1329, 1422
MlyI GAGTC 3 cut(s) 296, 919, 1151
MmeI TCCRAC 2 cut(s) 378, 970
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 3 cut(s) 720, 860, 1322
MslI CAYNNNNRTG 2 cut(s) 1117, 1343
MspA1I CMGCKG 2 cut(s) 535, 613
MspI CCGG 1 cut(s) 1289
MspR9I CCNGG 2 cut(s) 770, 1368
MvaI CCWGG 2 cut(s) 770, 1368
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 3 cut(s) 521, 541, 1026
NdeII GATC 3 cut(s) 214, 883, 1070
NlaIII CATG 6 cut(s) 449, 635, 768, 890, 1029, 1101
NlaIV GGNNCC 1 cut(s) 1093
NmuCI GTSAC 3 cut(s) 643, 894, 1154
NruI TCGCGA 1 cut(s) 69
NsbI TGCGCA 1 cut(s) 869
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 4 cut(s) 313, 365, 551, 1399
PkrI GCNGC 7 cut(s) 23, 40, 43, 1009, 1022, 1111, 1124
PleI GAGTC 3 cut(s) 295, 918, 1151
PpsI GAGTC 3 cut(s) 295, 918, 1151
PshAI GACNNNNGTC 1 cut(s) 960
Psp6I CCWGG 2 cut(s) 768, 1366
PspGI CCWGG 2 cut(s) 768, 1366
PspN4I GGNNCC 1 cut(s) 1093
PspPI GGNCC 1 cut(s) 1370
PstI CTGCAG 1 cut(s) 195
PvuII CAGCTG 2 cut(s) 535, 613
RruI TCGCGA 1 cut(s) 69
RsaI GTAC 1 cut(s) 1351
RsaNI GTAC 1 cut(s) 1350
RseI CAYNNNNRTG 2 cut(s) 1117, 1343
SaqAI TTAA 3 cut(s) 720, 860, 1322
SatI GCNGC 7 cut(s) 22, 39, 42, 1008, 1021, 1110, 1123
Sau3AI GATC 3 cut(s) 214, 883, 1070
Sau96I GGNCC 1 cut(s) 1370
SchI GAGTC 3 cut(s) 296, 919, 1151
ScrFI CCNGG 2 cut(s) 770, 1368
SduI GDGCHC 3 cut(s) 169, 764, 1267
SfaNI GCATC 6 cut(s) 235, 441, 524, 932, 980, 1121
SfcI CTRYAG 2 cut(s) 191, 1413
SmiMI CAYNNNNRTG 2 cut(s) 1117, 1343
SmlI CTYRAG 2 cut(s) 419, 825
SmoI CTYRAG 2 cut(s) 419, 825
SpeI ACTAGT 2 cut(s) 146, 1267
Sse9I AATT 6 cut(s) 624, 857, 979, 1036, 1329, 1422
SsiI CCGC 5 cut(s) 19, 22, 36, 39, 42
SspMI CTAG 4 cut(s) 147, 242, 362, 1268
StyD4I CCNGG 2 cut(s) 768, 1366
StyI CCWWGG 1 cut(s) 1074
TaaI ACNGT 6 cut(s) 164, 226, 490, 988, 1216, 1414
TaiI ACGT 1 cut(s) 685
TaqI TCGA 6 cut(s) 75, 81, 210, 499, 1069, 1151
TasI AATT 6 cut(s) 624, 857, 979, 1036, 1329, 1422
TatI WGTACW 1 cut(s) 1349
TauI GCSGC 3 cut(s) 24, 41, 44
TfiI GAWTC 4 cut(s) 313, 365, 551, 1399
Tru1I TTAA 3 cut(s) 720, 860, 1322
Tru9I TTAA 3 cut(s) 720, 860, 1322
TscAI CASTG 5 cut(s) 169, 744, 1213, 1221, 1437
TseFI GTSAC 3 cut(s) 643, 894, 1154
TseI GCWGC 4 cut(s) 1007, 1020, 1109, 1122
Tsp45I GTSAC 3 cut(s) 643, 894, 1154
TspDTI ATGAA 6 cut(s) 20, 129, 371, 693, 741, 1070
TspRI CASTG 5 cut(s) 169, 744, 1213, 1221, 1437
VneI GTGCAC 1 cut(s) 1263
XapI RAATTY 2 cut(s) 979, 1036
XmiI GTMKAC 1 cut(s) 1416
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 4 cut(s) 147, 242, 362, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.