FvH4_3g16210

glutathione s-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000304
Physical Location & Seq
Forward (+)
10230064 .. 10231584
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16210.t1

Sequence Viewer

Length: 663 bp
ATGGCGGAGGAGGTTGTTCTTCTGGGCTTCTGGTCGAGCATGTTCGCCATGAGACCCAGAGTTGCTCTGGCGGAGAAGGGCGTGGAGTATGAGTACAGAGAGGAAGACTTGCCCAACAAAACCTCACTGCTTCTGGAGATGAACCCGATCCACAAGAAGGTCCCAGTTCTTATCCACAACGGCAAACCGGTCTGTGAGTCGGTCAACATCGTCCAGTATATCGACGAGGCTTGGAAGGACAAAGGTCCTACTTTGCTTCCCTCCGATCCTTACGAGAGAGCTCAGGCCAGGTTCTGGGTTGATTACATTGATAAAAAGTTATATGAAGCTGGGAAGAGTATATGGTCCACAAAGGGAGAAGAACAAGAAGCAGCCAAGAAGGTTTTTCTTGAAATCCTTAAGCTGCTGGAGGGAGAACTTGGGGACAAGACGTACTTTGGGGGAGTGAGCTTCGGGTTCGTAGACATCAATCTTGTCACATTTTACTGCTGGTTTTATACCTATGAGACCATCGGAAACTTCAGCATAGAGGCAGAGTGCCCCAAACTAATTGCATGGTTCGAAAAGTGTATGCTGAAGGAGAGTGTCTCCAAAACTCTTCCAGACCCAAAAAAGGTGTATGAATTTTGCCTTTTCTTGCAGAAGTATTATGGTGTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016740 GO:0016765 GO:0018973 GO:0018974 GO:0019326 GO:0019439 GO:0019748 GO:0019899 GO:0031090 GO:0031668 GO:0033218 GO:0033554 GO:0034641 GO:0042221 GO:0042277 GO:0042537 GO:0042631 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046256 GO:0046260 GO:0046263 GO:0046686 GO:0048037 GO:0048046 GO:0048583 GO:0050789 GO:0050896 GO:0051186 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071944 GO:0072341 GO:0072490 GO:0072491 GO:0080167 GO:0098588 GO:0098754 GO:0098805 GO:0104004 GO:1900750 GO:1901360 GO:1901361 GO:1901564 GO:1901575 GO:1901681 GO:1901700 GO:1901701 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

221

Amino Acids

25.47

Weight (kDa)

5.19

Isoelectric Point (pI)

33.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 1.2e-20 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 77 3.6e-14 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 7e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 117 - 191 1.1e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 122 - 188 1.7e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 294
AccI GTMKAC 1 cut(s) 462
AciI CCGC 2 cut(s) 5, 71
AclWI GGATC 2 cut(s) 142, 260
AcsI RAATTY 1 cut(s) 623
AcuI CTGAAG 2 cut(s) 505, 596
AfaI GTAC 2 cut(s) 95, 434
AfiI CCNNNNNNNGG 3 cut(s) 157, 294, 613
AflII CTTAAG 1 cut(s) 398
AgeI ACCGGT 1 cut(s) 187
AgsI TTSAA 1 cut(s) 392
AjnI CCWGG 1 cut(s) 287
AluBI AGCT 4 cut(s) 281, 329, 403, 450
AluI AGCT 4 cut(s) 281, 329, 403, 450
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 3 cut(s) 46, 500, 592
AlwI GGATC 2 cut(s) 142, 260
AlwNI CAGNNNCTG 1 cut(s) 294
AoxI GGCC 2 cut(s) 285, 657
ApeKI GCWGC 2 cut(s) 371, 403
ApoI RAATTY 1 cut(s) 623
AsiGI ACCGGT 1 cut(s) 187
AspS9I GGNCC 3 cut(s) 160, 245, 345
AsuII TTCGAA 1 cut(s) 561
AvaII GGWCC 3 cut(s) 160, 245, 345
BaeGI GKGCMC 1 cut(s) 542
BanII GRGCYC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 2 cut(s) 383, 390
BccI CCATC 1 cut(s) 518
BceAI ACGGC 1 cut(s) 196
BciT130I CCWGG 1 cut(s) 289
BcoDI GTCTC 3 cut(s) 46, 500, 592
BfaI CTAG 1 cut(s) 661
BfrI CTTAAG 1 cut(s) 398
BisI GCNGC 2 cut(s) 372, 404
BlsI GCNGC 2 cut(s) 373, 405
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 3 cut(s) 160, 245, 345
BmgT120I GGNCC 3 cut(s) 160, 245, 345
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 289
BmrI ACTGGG 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 158
BoxI GACNNNNGTC 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 111
BplI GAGNNNNNCTC 2 cut(s) 572, 604
BpmI CTGGAG 2 cut(s) 155, 428
Bpu10I CCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 1 cut(s) 561
BsaI GGTCTC 2 cut(s) 46, 500
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 294, 613
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 2 cut(s) 164, 214
BseBI CCWGG 1 cut(s) 289
BseLI CCNNNNNNNGG 3 cut(s) 157, 294, 613
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 2 cut(s) 164, 214
BseRI GAGGAG 1 cut(s) 23
BseSI GKGCMC 1 cut(s) 542
BseXI GCAGC 2 cut(s) 383, 390
BseYI CCCAGC 1 cut(s) 329
BshFI GGCC 2 cut(s) 287, 659
BshTI ACCGGT 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 283
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 2 cut(s) 146, 437
BslI CCNNNNNNNGG 3 cut(s) 157, 294, 613
BsmAI GTCTC 3 cut(s) 46, 500, 592
BsmFI GGGAC 2 cut(s) 146, 437
BsnI GGCC 2 cut(s) 287, 659
Bso31I GGTCTC 2 cut(s) 46, 500
Bsp119I TTCGAA 1 cut(s) 561
Bsp1286I GDGCHC 2 cut(s) 283, 542
Bsp143I GATC 2 cut(s) 147, 265
BspACI CCGC 2 cut(s) 5, 71
BspANI GGCC 2 cut(s) 287, 659
BspCNI CTCAG 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 162
BspPI GGATC 2 cut(s) 142, 260
BspT104I TTCGAA 1 cut(s) 561
BspTI CTTAAG 1 cut(s) 398
BspTNI GGTCTC 2 cut(s) 46, 500
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 2 cut(s) 164, 214
BssAI RCCGGY 1 cut(s) 187
BssMI GATC 2 cut(s) 147, 265
Bst2UI CCWGG 1 cut(s) 289
Bst6I CTCTTC 2 cut(s) 329, 603
BstAFI CTTAAG 1 cut(s) 398
BstBI TTCGAA 1 cut(s) 561
BstDEI CTNAG 1 cut(s) 282
BstKTI GATC 2 cut(s) 150, 268
BstMAI GTCTC 3 cut(s) 46, 500, 592
BstMBI GATC 2 cut(s) 147, 265
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 1 cut(s) 43
BstPAI GACNNNNGTC 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 287
BstSLI GKGCMC 1 cut(s) 542
BstV1I GCAGC 2 cut(s) 383, 390
BstV2I GAAGAC 1 cut(s) 111
BsuRI GGCC 2 cut(s) 287, 659
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 1 cut(s) 125
CaiI CAGNNNCTG 1 cut(s) 294
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 160, 245, 345
Csp6I GTAC 2 cut(s) 94, 433
CspAI ACCGGT 1 cut(s) 187
CviAII CATG 3 cut(s) 40, 49, 555
CviJI RGCY 9 cut(s) 27, 230, 281, 287, 329, 374, 403, 450, 659
CviKI_1 RGCY 9 cut(s) 27, 230, 281, 287, 329, 374, 403, 450, 659
CviQI GTAC 2 cut(s) 94, 433
DdeI CTNAG 1 cut(s) 282
DpnI GATC 2 cut(s) 149, 267
DpnII GATC 2 cut(s) 147, 265
Eam1104I CTCTTC 2 cut(s) 329, 603
EarI CTCTTC 2 cut(s) 329, 603
EciI GGCGGA 2 cut(s) 20, 86
Ecl136II GAGCTC 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 283
Eco31I GGTCTC 2 cut(s) 46, 500
Eco47I GGWCC 3 cut(s) 160, 245, 345
Eco53kI GAGCTC 1 cut(s) 281
Eco57I CTGAAG 2 cut(s) 505, 596
EcoICRI GAGCTC 1 cut(s) 281
EcoO109I RGGNCCY 2 cut(s) 160, 245
EcoRII CCWGG 1 cut(s) 287
EcoT38I GRGCYC 1 cut(s) 283
FaeI CATG 3 cut(s) 43, 52, 558
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 2 cut(s) 146, 437
FatI CATG 3 cut(s) 39, 48, 554
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 2 cut(s) 372, 404
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 2 cut(s) 372, 404
FspBI CTAG 1 cut(s) 661
GluI GCNGC 2 cut(s) 372, 404
GsaI CCCAGC 1 cut(s) 333
GsuI CTGGAG 2 cut(s) 155, 428
HaeIII GGCC 2 cut(s) 287, 659
HapII CCGG 1 cut(s) 188
Hin1II CATG 3 cut(s) 43, 52, 558
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 1 cut(s) 197
HpaII CCGG 1 cut(s) 188
Hpy166II GTNNAC 3 cut(s) 205, 348, 463
Hpy188I TCNGA 2 cut(s) 265, 515
Hpy188III TCNNGA 3 cut(s) 134, 389, 602
Hpy8I GTNNAC 3 cut(s) 205, 348, 463
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 5 cut(s) 70, 151, 229, 373, 571
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 2 cut(s) 554, 640
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 3 cut(s) 43, 52, 558
Kzo9I GATC 2 cut(s) 147, 265
Lsp1109I GCAGC 2 cut(s) 383, 390
MaeI CTAG 1 cut(s) 661
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 1 cut(s) 475
MalI GATC 2 cut(s) 149, 267
MboI GATC 2 cut(s) 147, 265
MboII GAAGA 5 cut(s) 11, 116, 346, 371, 590
MhlI GDGCHC 2 cut(s) 283, 542
MluCI AATT 2 cut(s) 549, 623
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 7 cut(s) 4, 94, 133, 220, 271, 403, 523
MseI TTAA 1 cut(s) 399
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 1 cut(s) 188
MspR9I CCNGG 1 cut(s) 289
MvaI CCWGG 1 cut(s) 289
NdeII GATC 2 cut(s) 147, 265
NlaIII CATG 3 cut(s) 43, 52, 558
NlaIV GGNNCC 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 475
NspI RCATGY 1 cut(s) 43
NspV TTCGAA 1 cut(s) 561
PflMI CCANNNNNTGG 1 cut(s) 294
PinAI ACCGGT 1 cut(s) 187
PkrI GCNGC 2 cut(s) 373, 405
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PpuMI RGGWCCY 2 cut(s) 160, 245
PshAI GACNNNNGTC 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 283
Psp5II RGGWCCY 2 cut(s) 160, 245
Psp6I CCWGG 1 cut(s) 287
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 1 cut(s) 162
PspPI GGNCC 3 cut(s) 160, 245, 345
PspPPI RGGWCCY 2 cut(s) 160, 245
PstNI CAGNNNCTG 1 cut(s) 294
RsaI GTAC 2 cut(s) 95, 434
RsaNI GTAC 2 cut(s) 94, 433
SacI GAGCTC 1 cut(s) 283
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 2 cut(s) 372, 404
Sau3AI GATC 2 cut(s) 147, 265
Sau96I GGNCC 3 cut(s) 160, 245, 345
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 2 cut(s) 283, 542
SfuI TTCGAA 1 cut(s) 561
SinI GGWCC 3 cut(s) 160, 245, 345
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 2 cut(s) 549, 623
SsiI CCGC 2 cut(s) 5, 71
SspMI CTAG 1 cut(s) 661
SstI GAGCTC 1 cut(s) 283
StyD4I CCNGG 1 cut(s) 287
TaiI ACGT 1 cut(s) 434
TaqI TCGA 3 cut(s) 35, 222, 561
TaqII GACCGA 1 cut(s) 190
TasI AATT 2 cut(s) 549, 623
TatI WGTACW 1 cut(s) 93
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 1 cut(s) 132
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 2 cut(s) 371, 403
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 3 cut(s) 155, 339, 636
TspRI CASTG 1 cut(s) 132
Van91I CCANNNNNTGG 1 cut(s) 294
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 3 cut(s) 160, 245, 345
XapI RAATTY 1 cut(s) 623
XceI RCATGY 1 cut(s) 43
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 462
XspI CTAG 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.