Rw5G017550

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000304
Physical Location & Seq
Reverse (-)
22429894 .. 22430657
764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017550.1

Sequence Viewer

Length: 651 bp
ATGGCAGATGAGGTTGCGTTGTTGGATTTCTGGCCGAGTCCATTTGGGATGAGGCTGAGGATCGCTCTAGCTGAGAAAAGCATCAAGCACGAGTACAAGGAGGAAGACCTCAAGAACAAGAGCCCACTTTTGCTGAAGTCGAACCCGGTTCACAAGAAGATCCCGGTTCTCATTCACAATGGCAAACCCATCAGTGAATCCCTCATTGCCCTCCAGTACATTGACGAAACTTGGAATGACAACGCTCCACTGTTGCCCTCTGATCCTTATCTCAGATCCCAAGCCAGGTTCTGGGCCGAATTTGTTGACAAAAAGGTTGCTGATTTTGGGAGGAAGGTATGCATGACCAAAGGAGAAGAACAGGAGGCAGCAAAGAAAGGCCTACTTGATTGCATTGAACTACTGGAAGGAGAACTTGGAGAGAAGCCTTACTTTGGGGGTGAGACCCTTGGTTTCGTGGACGTGACACTCGTTCCCTGGTATGGTTGGCTTTCCGTGTACGAGAAGTTTGGCAACTTCAGTATAGAGGCAGAGCACCCAGTATTCATAGCTTGGGGGAAGAGGTGTATGCAGAAGAAGAGTGTGTCCAAGTCTCTTCCTGACCAGCAAAAGCTCTATGACTTCGTTGTGCAATTCATGGAAAAGGCTTGA

Protein Analysis

216

Amino Acids

24.76

Weight (kDa)

5.89

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 1.9e-17 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 12 - 78 2.5e-11 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 77 7.4e-12 Glutathione S-transferase, N-terminal domain
GST_C PF00043 117 - 191 2.7e-07 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 121 - 185 6.4e-09 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 291
AclWI GGATC 4 cut(s) 68, 154, 257, 270
AcoI YGGCCR 1 cut(s) 32
AcsI RAATTY 1 cut(s) 299
AcuI CTGAAG 2 cut(s) 155, 502
AfaI GTAC 3 cut(s) 95, 218, 500
AfiI CCNNNNNNNGG 4 cut(s) 285, 291, 434, 482
AgsI TTSAA 1 cut(s) 398
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 2 cut(s) 284, 476
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 3 cut(s) 71, 551, 613
AluI AGCT 3 cut(s) 71, 551, 613
Alw21I GWGCWC 1 cut(s) 537
Alw26I GTCTC 2 cut(s) 437, 597
AlwI GGATC 4 cut(s) 68, 154, 257, 270
AlwNI CAGNNNCTG 1 cut(s) 291
AoxI GGCC 3 cut(s) 32, 294, 379
ApeKI GCWGC 1 cut(s) 368
ApoI RAATTY 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 294
AsuC2I CCSGG 2 cut(s) 146, 164
AsuHPI GGTGA 1 cut(s) 452
BanII GRGCYC 1 cut(s) 125
BauI CACGAG 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 537
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 380
BccI CCATC 1 cut(s) 197
BciT130I CCWGG 2 cut(s) 286, 478
BcnI CCSGG 2 cut(s) 146, 164
BcoDI GTCTC 2 cut(s) 437, 597
BfaI CTAG 1 cut(s) 68
BisI GCNGC 1 cut(s) 369
BlsI GCNGC 1 cut(s) 370
Bme1390I CCNGG 4 cut(s) 146, 164, 286, 478
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 1 cut(s) 294
BmrFI CCNGG 4 cut(s) 146, 164, 286, 478
BmrI ACTGGG 1 cut(s) 533
BmsI GCATC 1 cut(s) 90
BmuI ACTGGG 1 cut(s) 533
BpiI GAAGAC 1 cut(s) 111
BplI GAGNNNNNCTC 2 cut(s) 49, 81
BpmI CTGGAG 1 cut(s) 197
Bpu10I CCTNAGC 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 2 cut(s) 146, 164
BsaBI GATNNNNATC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 448, 476
Bsc4I CCNNNNNNNGG 4 cut(s) 285, 291, 434, 482
Bse1I ACTGG 3 cut(s) 214, 408, 539
Bse3DI GCAATG 1 cut(s) 204
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 2 cut(s) 286, 478
BseDI CCNNGG 2 cut(s) 448, 476
BseGI GGATG 1 cut(s) 54
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 4 cut(s) 285, 291, 434, 482
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 3 cut(s) 47, 63, 286
BseNI ACTGG 3 cut(s) 214, 408, 539
BseXI GCAGC 1 cut(s) 380
BshFI GGCC 3 cut(s) 34, 296, 381
BsiHKAI GWGCWC 1 cut(s) 537
BsiSI CCGG 2 cut(s) 146, 164
BslI CCNNNNNNNGG 4 cut(s) 285, 291, 434, 482
BsmAI GTCTC 2 cut(s) 437, 597
BsnI GGCC 3 cut(s) 34, 296, 381
Bso31I GGTCTC 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 125, 537
Bsp143I GATC 4 cut(s) 60, 159, 262, 275
BspANI GGCC 3 cut(s) 34, 296, 381
BspCNI CTCAG 3 cut(s) 48, 64, 285
BspPI GGATC 4 cut(s) 68, 154, 257, 270
BspTNI GGTCTC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 3 cut(s) 214, 408, 539
BssECI CCNNGG 2 cut(s) 448, 476
BssMI GATC 4 cut(s) 60, 159, 262, 275
BssSI CACGAG 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 448
Bst2BI CACGAG 1 cut(s) 89
Bst2UI CCWGG 2 cut(s) 286, 478
Bst4CI ACNGT 1 cut(s) 252
Bst6I CTCTTC 3 cut(s) 554, 572, 600
BstDEI CTNAG 3 cut(s) 56, 72, 272
BstF5I GGATG 1 cut(s) 54
BstKTI GATC 4 cut(s) 63, 162, 265, 278
BstMAI GTCTC 2 cut(s) 437, 597
BstMBI GATC 4 cut(s) 60, 159, 262, 275
BstNI CCWGG 2 cut(s) 286, 478
BstSCI CCNGG 4 cut(s) 144, 162, 284, 476
BstV1I GCAGC 1 cut(s) 380
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 2 cut(s) 159, 275
BstYI RGATCY 2 cut(s) 159, 275
BsuRI GGCC 3 cut(s) 34, 296, 381
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 199, 248
CaiI CAGNNNCTG 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 294
Csp6I GTAC 3 cut(s) 94, 217, 499
CviAII CATG 2 cut(s) 343, 637
CviQI GTAC 3 cut(s) 94, 217, 499
DdeI CTNAG 3 cut(s) 56, 72, 272
DpnI GATC 4 cut(s) 62, 161, 264, 277
DpnII GATC 4 cut(s) 60, 159, 262, 275
EaeI YGGCCR 1 cut(s) 32
Eam1104I CTCTTC 3 cut(s) 554, 572, 600
EarI CTCTTC 3 cut(s) 554, 572, 600
Eco130I CCWWGG 1 cut(s) 448
Eco147I AGGCCT 1 cut(s) 381
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 437
Eco57I CTGAAG 2 cut(s) 155, 502
EcoRII CCWGG 2 cut(s) 284, 476
EcoT14I CCWWGG 1 cut(s) 448
EcoT22I ATGCAT 1 cut(s) 344
EcoT38I GRGCYC 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 448
FaeI CATG 2 cut(s) 346, 640
FaiI YATR 8 cut(s) 340, 344, 483, 524, 548, 569, 618, 638
FalI AAGNNNNNCTT 6 cut(s) 369, 401, 399, 431, 416, 448
FatI CATG 2 cut(s) 342, 636
Fnu4HI GCNGC 1 cut(s) 369
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 1 cut(s) 369
FspBI CTAG 1 cut(s) 68
GluI GCNGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 197
HaeIII GGCC 3 cut(s) 34, 296, 381
HapII CCGG 2 cut(s) 146, 164
Hin1II CATG 2 cut(s) 346, 640
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 2 cut(s) 37, 197
HpaII CCGG 2 cut(s) 146, 164
HphI GGTGA 1 cut(s) 452
Hpy166II GTNNAC 4 cut(s) 151, 307, 460, 499
Hpy188I TCNGA 2 cut(s) 262, 275
Hpy188III TCNNGA 2 cut(s) 112, 599
Hpy8I GTNNAC 4 cut(s) 151, 307, 460, 499
HpyAV CCTTC 2 cut(s) 328, 401
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 4 cut(s) 342, 393, 571, 631
HpyF3I CTNAG 3 cut(s) 56, 72, 272
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 2 cut(s) 346, 640
Kzo9I GATC 4 cut(s) 60, 159, 262, 275
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 1 cut(s) 380
LweI GCATC 1 cut(s) 90
MaeI CTAG 1 cut(s) 68
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 1 cut(s) 463
MalI GATC 4 cut(s) 62, 161, 264, 277
MboI GATC 4 cut(s) 60, 159, 262, 275
MboII GAAGA 7 cut(s) 116, 169, 368, 571, 586, 587, 589
MflI RGATCY 2 cut(s) 159, 275
MhlI GDGCHC 2 cut(s) 125, 537
MluCI AATT 2 cut(s) 299, 632
MlyI GAGTC 1 cut(s) 46
Mph1103I ATGCAT 1 cut(s) 344
MspI CCGG 2 cut(s) 146, 164
MspR9I CCNGG 4 cut(s) 146, 164, 286, 478
MvaI CCWGG 2 cut(s) 286, 478
NciI CCSGG 2 cut(s) 146, 164
NdeII GATC 4 cut(s) 60, 159, 262, 275
NlaIII CATG 2 cut(s) 346, 640
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 1 cut(s) 463
NsiI ATGCAT 1 cut(s) 344
PceI AGGCCT 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 468
PfeI GAWTC 1 cut(s) 197
PflMI CCANNNNNTGG 1 cut(s) 291
PkrI GCNGC 1 cut(s) 370
PleI GAGTC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 45
Psp6I CCWGG 2 cut(s) 284, 476
PspGI CCWGG 2 cut(s) 284, 476
PspPI GGNCC 1 cut(s) 294
PstNI CAGNNNCTG 1 cut(s) 291
PsuI RGATCY 2 cut(s) 159, 275
RsaI GTAC 3 cut(s) 95, 218, 500
RsaNI GTAC 3 cut(s) 94, 217, 499
SatI GCNGC 1 cut(s) 369
Sau3AI GATC 4 cut(s) 60, 159, 262, 275
Sau96I GGNCC 1 cut(s) 294
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 4 cut(s) 146, 164, 286, 478
SduI GDGCHC 2 cut(s) 125, 537
SfaNI GCATC 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 2 cut(s) 299, 632
SseBI AGGCCT 1 cut(s) 381
SspMI CTAG 1 cut(s) 68
StuI AGGCCT 1 cut(s) 381
StyD4I CCNGG 4 cut(s) 144, 162, 284, 476
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 1 cut(s) 252
TaiI ACGT 1 cut(s) 465
TaqI TCGA 1 cut(s) 140
TasI AATT 2 cut(s) 299, 632
TatI WGTACW 2 cut(s) 93, 216
TfiI GAWTC 1 cut(s) 197
TscAI CASTG 2 cut(s) 199, 255
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 1 cut(s) 368
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 2 cut(s) 535, 625
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 2 cut(s) 199, 255
Van91I CCANNNNNTGG 1 cut(s) 291
XapI RAATTY 1 cut(s) 299
XspI CTAG 1 cut(s) 68
Zsp2I ATGCAT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.