Rh5BG190100

glutathione s-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000304
Physical Location & Seq
Forward (+)
20989973 .. 20993731
3759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG190100.1

Sequence Viewer

Length: 396 bp
ATGGCAGAAGGCAAGGGGATTGGATTGGAGAAAGGATCATGCAGGAAGATATGGTTTACAAAAGGAGAAGAGCTGCAGGCAGCAAAGAAGGAATTCATTGAATGCCTGAAGGTGTTGGAAGGCGAGCTTGGAGACAAGCCTTATTTCATGGGTGAGAGATTTGGGTTCCTGGACATTGCTCTCATCACATTCTACACTTGGTTTCATGCTTATGAGACACTTGGAAACTTCAGTATAGAGGCTGAGTGCCCCAAGCTGATTTCATGGGCCAAGAGGTGCTTGCAGACGGAGAGTGTTTCAAAATCTCTTACTGACCAGAAAAAGGCTTATGAGTATCTTCTTGAGATGAAGAAGAGGCTTGGTGTGGAATGGTGGTATAAGAAAGTCCTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016740 GO:0016765 GO:0018973 GO:0018974 GO:0019326 GO:0019439 GO:0019748 GO:0019899 GO:0031090 GO:0031668 GO:0033218 GO:0033554 GO:0034641 GO:0042221 GO:0042277 GO:0042537 GO:0042631 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046256 GO:0046260 GO:0046263 GO:0046686 GO:0048037 GO:0048046 GO:0048583 GO:0050789 GO:0050896 GO:0051186 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071944 GO:0072341 GO:0072490 GO:0072491 GO:0080167 GO:0098588 GO:0098754 GO:0098805 GO:0104004 GO:1900750 GO:1901360 GO:1901361 GO:1901564 GO:1901575 GO:1901681 GO:1901700 GO:1901701 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

131

Amino Acids

15.3

Weight (kDa)

8.59

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_C PF00043 21 - 95 1e-08 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 24 - 92 5.6e-12 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 43
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 2 cut(s) 128, 214
AfiI CCNNNNNNNGG 1 cut(s) 322
AgsI TTSAA 2 cut(s) 101, 300
AjnI CCWGG 1 cut(s) 168
AjuI GAANNNNNNNTTGG 2 cut(s) 111, 143
AluBI AGCT 3 cut(s) 73, 127, 256
AluI AGCT 3 cut(s) 73, 127, 256
Alw26I GTCTC 2 cut(s) 126, 209
AlwI GGATC 1 cut(s) 43
AoxI GGCC 1 cut(s) 267
ApeKI GCWGC 2 cut(s) 73, 80
ApoI RAATTY 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 267
AsuHPI GGTGA 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 251
BbvI GCAGC 2 cut(s) 60, 92
BciT130I CCWGG 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 126, 209
BfmI CTRYAG 1 cut(s) 74
BisI GCNGC 2 cut(s) 74, 81
BlsI GCNGC 2 cut(s) 75, 82
Bme1390I CCNGG 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 1 cut(s) 167
BmrFI CCNGG 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 362
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse3DI GCAATG 1 cut(s) 174
BseBI CCWGG 1 cut(s) 170
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 1 cut(s) 234
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 2 cut(s) 60, 92
BshFI GGCC 1 cut(s) 269
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 2 cut(s) 126, 209
BsmI GAATGC 1 cut(s) 107
BsnI GGCC 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 1 cut(s) 35
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 167
BspMAI CTGCAG 1 cut(s) 78
BspPI GGATC 1 cut(s) 43
BspQI GCTCTTC 1 cut(s) 63
BsrDI GCAATG 1 cut(s) 174
BssMI GATC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 170
Bst6I CTCTTC 2 cut(s) 63, 347
BstC8I GCNNGC 3 cut(s) 78, 125, 281
BstDEI CTNAG 1 cut(s) 243
BstKTI GATC 1 cut(s) 38
BstMAI GTCTC 2 cut(s) 126, 209
BstMBI GATC 1 cut(s) 35
BstNI CCWGG 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 168
BstSFI CTRYAG 1 cut(s) 74
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 2 cut(s) 60, 92
BsuRI GGCC 1 cut(s) 269
Cac8I GCNNGC 3 cut(s) 78, 125, 281
Cfr13I GGNCC 1 cut(s) 267
CviAII CATG 4 cut(s) 39, 148, 206, 264
CviJI RGCY 8 cut(s) 73, 127, 139, 242, 256, 269, 326, 358
CviKI_1 RGCY 8 cut(s) 73, 127, 139, 242, 256, 269, 326, 358
DdeI CTNAG 1 cut(s) 243
DpnI GATC 1 cut(s) 37
DpnII GATC 1 cut(s) 35
Eam1104I CTCTTC 2 cut(s) 63, 347
EarI CTCTTC 2 cut(s) 63, 347
Eco57I CTGAAG 2 cut(s) 128, 214
EcoRI GAATTC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 168
FaeI CATG 4 cut(s) 42, 151, 209, 267
FaiI YATR 9 cut(s) 40, 52, 149, 207, 213, 236, 265, 330, 378
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 263, 295
FatI CATG 4 cut(s) 38, 147, 205, 263
Fnu4HI GCNGC 2 cut(s) 74, 81
Fsp4HI GCNGC 2 cut(s) 74, 81
GluI GCNGC 2 cut(s) 74, 81
HaeIII GGCC 1 cut(s) 269
Hin1II CATG 4 cut(s) 42, 151, 209, 267
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 1 cut(s) 57
Hpy188III TCNNGA 2 cut(s) 341, 388
Hpy8I GTNNAC 1 cut(s) 57
HpyAV CCTTC 3 cut(s) 82, 103, 113
HpyCH4V TGCA 3 cut(s) 42, 76, 283
HpyF3I CTNAG 1 cut(s) 243
Hsp92II CATG 4 cut(s) 42, 151, 209, 267
Kzo9I GATC 1 cut(s) 35
LguI GCTCTTC 1 cut(s) 63
LpnPI CCDG 6 cut(s) 28, 62, 119, 155, 182, 329
Lsp1109I GCAGC 2 cut(s) 60, 92
MalI GATC 1 cut(s) 37
MboI GATC 1 cut(s) 35
MboII GAAGA 5 cut(s) 58, 80, 329, 361, 364
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 3 cut(s) 232, 267, 348
MroXI GAANNNNTTC 1 cut(s) 92
MslI CAYNNNNRTG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 170
Mva1269I GAATGC 1 cut(s) 107
MvaI CCWGG 1 cut(s) 170
NdeII GATC 1 cut(s) 35
NlaIII CATG 4 cut(s) 42, 151, 209, 267
NlaIV GGNNCC 1 cut(s) 167
PciSI GCTCTTC 1 cut(s) 63
PctI GAATGC 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 92
PfoI TCCNGGA 1 cut(s) 168
PkrI GCNGC 2 cut(s) 75, 82
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 167
PspPI GGNCC 1 cut(s) 267
PstI CTGCAG 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 63
SatI GCNGC 2 cut(s) 74, 81
Sau3AI GATC 1 cut(s) 35
Sau96I GGNCC 1 cut(s) 267
ScrFI CCNGG 1 cut(s) 170
SduI GDGCHC 1 cut(s) 251
SetI ASST 5 cut(s) 75, 114, 129, 258, 278
SfcI CTRYAG 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 1 cut(s) 92
StyD4I CCNGG 1 cut(s) 168
TasI AATT 1 cut(s) 92
TseI GCWGC 2 cut(s) 73, 80
TspDTI ATGAA 5 cut(s) 85, 136, 194, 252, 362
TspGWI ACGGA 1 cut(s) 302
XapI RAATTY 1 cut(s) 92
XmnI GAANNNNTTC 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.