Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000304

Physical Location & Seq

Genomic Coordinates Forward (+)

34122009 .. 34124732

Gene Structure 2724 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1209700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 921 bp

ATGGCAGAGGAAGTGGTTCTGTTGGATTTCTGGCCAAGCCCTTTTGGGATGAGGGTGAGGATTGCCCTGGCGGAGAAGGGCGTCGAGTACGAGTCCCGGGAAGAAGACTTGAGCAACAAGAGCCCTCTGCTGCTCAAGATGAACCCTGTTCACAAGCAAATCCCAGTTCTGATCCACAAGGGAAGACCGATATGCGAATCGCTCATCATTGTTCAGTACATTGATGAGGTTTGGAGTGATAAAGCTCCTCTGCTGCCTTCTGATCCTTACCCTAGAGCCCATGCAAGGTTCTGGGCTGACTATGTTGACAAGAAGATTTATTCAATAGGGAAGTTGGTGTGGGTAACCACAGGTGAAGTCCAAGAGGCAGCAAAGAAGGAACTGATAGAGTGCTTTAAGCTGCTGGAAAACGAGCTAGGGGACAAGACCTACTTTGGGGGTGAGAGTTTTGGCCTAGTGGACGTGGCACTTATCCCTTTCTATAGCTGGTTCTATGCGCTGGAGACCTGTGGAAACATGTGCATGGTGAAGGAGTGTCCGAGGCTGGTGGGTTGGGCAAAGAGATGCATGCAGAGGGAGAGCGTGTCCGAGTCTCTGCCCGATCAGTACAAAATTCCAAGAGGCAGCAAAGAAGGAACTGATAGAGAGTGCTTCAAGCTGCTGGAAAACGAGCTAGGGGACAAGGCCTACTTTCGGGGTGAGAGTTTTGTCCTAGTGGACGTGGCACTTATCCCTTTCTATAGCTGGTTCTATGCACTGGAGACCTGTGGAAACCTGTGCATGGTGAAGGAGTGTCCGAGGCTGGTGGGTTGGGCAAAGAGATGTATGCAGAGGGAGAGTGTGTCCAAGTCTCTTCCTGATCAGTACAAAATGTATGACTTCCTTTTGGAGCTTAGAAAGAAGTTTGATGTTCATACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009404 GO:0009407 GO:0009636 GO:0009987 GO:0016740 GO:0016765 GO:0019748 GO:0034641 GO:0042221 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044464 GO:0050896 GO:0051186 GO:0071704 GO:0098754 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

307

Amino Acids

35.44

Weight (kDa)

5.78

Isoelectric Point (pI)

43.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	5 - 76	7.2e-21	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	12 - 76	2.6e-14	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	13 - 76	9.3e-15	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	119 - 193	5.2e-10	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	120 - 188	1.1e-08	Glutathione S-transferase, C-terminal domain
GST_C	PF00043	212 - 279	1.2e-06	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000304)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca	FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica	MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica	Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis	pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis	RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata	RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora	Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii	Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa	Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha	Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana	Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	71
AclWI	GGATC	2 cut(s)	166, 257
AcoI	YGGCCR	1 cut(s)	32
AcsI	RAATTY	1 cut(s)	612
AcyI	GRCGYC	1 cut(s)	81
AfaI	GTAC	4 cut(s)	89, 218, 608, 866
AfiI	CCNNNNNNNGG	4 cut(s)	285, 435, 693, 781
AflIII	ACRYGT	1 cut(s)	516
AgsI	TTSAA	2 cut(s)	324, 655
AjiI	CACGTC	2 cut(s)	463, 721
AjnI	CCWGG	1 cut(s)	66
AluBI	AGCT	8 cut(s)	245, 400, 415, 486, 658, 673, 744, 892
AluI	AGCT	8 cut(s)	245, 400, 415, 486, 658, 673, 744, 892
Alw26I	GTCTC	4 cut(s)	497, 597, 755, 855
AlwI	GGATC	2 cut(s)	166, 257
Ama87I	CYCGRG	1 cut(s)	96
AoxI	GGCC	3 cut(s)	32, 451, 684
ApeKI	GCWGC	6 cut(s)	130, 253, 368, 400, 624, 658
ApoI	RAATTY	1 cut(s)	612
Asp700I	GAANNNNTTC	1 cut(s)	15
AspLEI	GCGC	1 cut(s)	499
AsuC2I	CCSGG	2 cut(s)	97, 98
AsuHPI	GGTGA	6 cut(s)	67, 365, 452, 538, 710, 796
AvaI	CYCGRG	1 cut(s)	96
BalI	TGGCCA	1 cut(s)	34
BanII	GRGCYC	2 cut(s)	125, 280
BbsI	GAAGAC	2 cut(s)	111, 190
BbvI	GCAGC	6 cut(s)	117, 240, 380, 387, 636, 645
BciT130I	CCWGG	1 cut(s)	68
BclI	TGATCA	1 cut(s)	859
BcnI	CCSGG	2 cut(s)	97, 98
BcoDI	GTCTC	4 cut(s)	497, 597, 755, 855
BfaI	CTAG	5 cut(s)	273, 416, 455, 674, 713
BfmI	CTRYAG	2 cut(s)	481, 739
BisI	GCNGC	6 cut(s)	131, 254, 369, 401, 625, 659
BlsI	GCNGC	6 cut(s)	132, 255, 370, 402, 626, 660
Bme1390I	CCNGG	3 cut(s)	68, 97, 98
BmeT110I	CYCGRG	1 cut(s)	96
BmgBI	CACGTC	2 cut(s)	463, 721
BmrFI	CCNGG	3 cut(s)	68, 97, 98
BmrI	ACTGGG	1 cut(s)	158
BmsI	GCATC	1 cut(s)	554
BmuI	ACTGGG	1 cut(s)	158
BpiI	GAAGAC	2 cut(s)	111, 190
BpmI	CTGGAG	2 cut(s)	521, 779
BpuEI	CTTGAG	2 cut(s)	119, 130
BpuMI	CCSGG	2 cut(s)	97, 98
BsaHI	GRCGYC	1 cut(s)	81
BsaI	GGTCTC	2 cut(s)	497, 755
BsaJI	CCNNGG	4 cut(s)	66, 96, 539, 797
BsaXI	ACNNNNNCTCC	4 cut(s)	569, 599, 827, 857
Bsc4I	CCNNNNNNNGG	4 cut(s)	285, 435, 693, 781
Bse1I	ACTGG	2 cut(s)	164, 762
BseBI	CCWGG	1 cut(s)	68
BseDI	CCNNGG	4 cut(s)	66, 96, 539, 797
BseGI	GGATG	1 cut(s)	54
BseLI	CCNNNNNNNGG	4 cut(s)	285, 435, 693, 781
BseNI	ACTGG	2 cut(s)	164, 762
BseRI	GAGGAG	1 cut(s)	237
BseXI	GCAGC	6 cut(s)	117, 240, 380, 387, 636, 645
BshFI	GGCC	3 cut(s)	34, 453, 686
BsiHKCI	CYCGRG	1 cut(s)	96
BsiSI	CCGG	1 cut(s)	97
BslFI	GGGAC	3 cut(s)	79, 434, 692
BslI	CCNNNNNNNGG	4 cut(s)	285, 435, 693, 781
BsmAI	GTCTC	4 cut(s)	497, 597, 755, 855
BsmFI	GGGAC	3 cut(s)	79, 434, 692
BsnI	GGCC	3 cut(s)	34, 453, 686
Bso31I	GGTCTC	2 cut(s)	497, 755
BsoBI	CYCGRG	1 cut(s)	96
Bsp1286I	GDGCHC	2 cut(s)	125, 280
Bsp143I	GATC	4 cut(s)	171, 262, 601, 859
BspACI	CCGC	1 cut(s)	71
BspANI	GGCC	3 cut(s)	34, 453, 686
BspPI	GGATC	2 cut(s)	166, 257
BspTNI	GGTCTC	2 cut(s)	497, 755
BsrI	ACTGG	2 cut(s)	164, 762
BssECI	CCNNGG	4 cut(s)	66, 96, 539, 797
BssMI	GATC	4 cut(s)	171, 262, 601, 859
BssNI	GRCGYC	1 cut(s)	81
Bst2UI	CCWGG	1 cut(s)	68
Bst6I	CTCTTC	1 cut(s)	858
BstACI	GRCGYC	1 cut(s)	81
BstC8I	GCNNGC	1 cut(s)	569
BstDEI	CTNAG	1 cut(s)	893
BstEII	GGTNACC	1 cut(s)	343
BstF5I	GGATG	1 cut(s)	54
BstHHI	GCGC	1 cut(s)	499
BstKTI	GATC	4 cut(s)	174, 265, 604, 862
BstMAI	GTCTC	4 cut(s)	497, 597, 755, 855
BstMBI	GATC	4 cut(s)	171, 262, 601, 859
BstMWI	GCNNNNNNNGC	1 cut(s)	120
BstNI	CCWGG	1 cut(s)	68
BstNSI	RCATGY	2 cut(s)	520, 571
BstPI	GGTNACC	1 cut(s)	343
BstSCI	CCNGG	3 cut(s)	66, 95, 96
BstSFI	CTRYAG	2 cut(s)	481, 739
BstV1I	GCAGC	6 cut(s)	117, 240, 380, 387, 636, 645
BstV2I	GAAGAC	2 cut(s)	111, 190
BsuRI	GGCC	3 cut(s)	34, 453, 686
BtrI	CACGTC	2 cut(s)	463, 721
BtsCI	GGATG	1 cut(s)	54
BtsIMutI	CAGTG	1 cut(s)	755
Cac8I	GCNNGC	1 cut(s)	569
CfoI	GCGC	1 cut(s)	499
Cfr9I	CCCGGG	1 cut(s)	96
CseI	GACGC	1 cut(s)	70
Csp6I	GTAC	4 cut(s)	88, 217, 607, 865
CviAII	CATG	5 cut(s)	281, 517, 523, 568, 781
CviQI	GTAC	4 cut(s)	88, 217, 607, 865
DdeI	CTNAG	1 cut(s)	893
DpnI	GATC	4 cut(s)	173, 264, 603, 861
DpnII	GATC	4 cut(s)	171, 262, 601, 859
EaeI	YGGCCR	1 cut(s)	32
Eam1104I	CTCTTC	1 cut(s)	858
EarI	CTCTTC	1 cut(s)	858
EciI	GGCGGA	1 cut(s)	86
Eco147I	AGGCCT	1 cut(s)	686
Eco24I	GRGCYC	2 cut(s)	125, 280
Eco31I	GGTCTC	2 cut(s)	497, 755
Eco88I	CYCGRG	1 cut(s)	96
Eco91I	GGTNACC	1 cut(s)	343
EcoO65I	GGTNACC	1 cut(s)	343
EcoRII	CCWGG	1 cut(s)	66
EcoT22I	ATGCAT	1 cut(s)	569
EcoT38I	GRGCYC	2 cut(s)	125, 280
FaeI	CATG	5 cut(s)	284, 520, 526, 571, 784
FalI	AAGNNNNNCTT	4 cut(s)	416, 448, 674, 706
FaqI	GGGAC	3 cut(s)	79, 434, 692
FatI	CATG	5 cut(s)	280, 516, 522, 567, 780
FbaI	TGATCA	1 cut(s)	859
Fnu4HI	GCNGC	6 cut(s)	131, 254, 369, 401, 625, 659
FokI	GGATG	1 cut(s)	61
FriOI	GRGCYC	2 cut(s)	125, 280
Fsp4HI	GCNGC	6 cut(s)	131, 254, 369, 401, 625, 659
FspBI	CTAG	5 cut(s)	273, 416, 455, 674, 713
GlaI	GCGC	1 cut(s)	498
GluI	GCNGC	6 cut(s)	131, 254, 369, 401, 625, 659
GsuI	CTGGAG	2 cut(s)	521, 779
HaeIII	GGCC	3 cut(s)	34, 453, 686
HapII	CCGG	1 cut(s)	97
HgaI	GACGC	1 cut(s)	70
HhaI	GCGC	1 cut(s)	499
Hin1I	GRCGYC	1 cut(s)	81
Hin1II	CATG	5 cut(s)	284, 520, 526, 571, 784
Hin6I	GCGC	1 cut(s)	497
HinP1I	GCGC	1 cut(s)	497
HincII	GTYRAC	1 cut(s)	307
HindII	GTYRAC	1 cut(s)	307
HinfI	GANTC	3 cut(s)	92, 197, 590
HpaII	CCGG	1 cut(s)	97
HphI	GGTGA	6 cut(s)	67, 365, 452, 538, 710, 796
Hpy166II	GTNNAC	4 cut(s)	151, 307, 460, 718
Hpy188I	TCNGA	5 cut(s)	171, 262, 540, 589, 798
Hpy188III	TCNNGA	2 cut(s)	136, 857
Hpy8I	GTNNAC	4 cut(s)	151, 307, 460, 718
Hpy99I	CGWCG	1 cut(s)	86
HpyAV	CCTTC	6 cut(s)	70, 267, 370, 523, 626, 781
HpyCH4IV	ACGT	2 cut(s)	462, 720
HpyCH4V	TGCA	7 cut(s)	284, 522, 567, 571, 755, 780, 829
HpyF10VI	GCNNNNNNNGC	1 cut(s)	120
HpyF3I	CTNAG	1 cut(s)	893
HpySE526I	ACGT	2 cut(s)	462, 720
Hsp92I	GRCGYC	1 cut(s)	81
Hsp92II	CATG	5 cut(s)	284, 520, 526, 571, 784
HspAI	GCGC	1 cut(s)	497
Ksp22I	TGATCA	1 cut(s)	859
Kzo9I	GATC	4 cut(s)	171, 262, 601, 859
LmnI	GCTCC	2 cut(s)	250, 889
Lsp1109I	GCAGC	6 cut(s)	117, 240, 380, 387, 636, 645
LweI	GCATC	1 cut(s)	554
MaeI	CTAG	5 cut(s)	273, 416, 455, 674, 713
MaeII	ACGT	2 cut(s)	462, 720
MaeIII	GTNAC	1 cut(s)	343
MalI	GATC	4 cut(s)	173, 264, 603, 861
MboI	GATC	4 cut(s)	171, 262, 601, 859
MboII	GAAGA	5 cut(s)	113, 116, 195, 325, 845
MhlI	GDGCHC	2 cut(s)	125, 280
MlsI	TGGCCA	1 cut(s)	34
MluCI	AATT	1 cut(s)	612
MluNI	TGGCCA	1 cut(s)	34
MlyI	GAGTC	2 cut(s)	101, 599
Mox20I	TGGCCA	1 cut(s)	34
Mph1103I	ATGCAT	1 cut(s)	569
MroXI	GAANNNNTTC	1 cut(s)	15
MscI	TGGCCA	1 cut(s)	34
MseI	TTAA	1 cut(s)	396
MslI	CAYNNNNRTG	1 cut(s)	521
Msp20I	TGGCCA	1 cut(s)	34
MspI	CCGG	1 cut(s)	97
MspR9I	CCNGG	3 cut(s)	68, 97, 98
MvaI	CCWGG	1 cut(s)	68
MwoI	GCNNNNNNNGC	1 cut(s)	120
NciI	CCSGG	2 cut(s)	97, 98
NdeII	GATC	4 cut(s)	171, 262, 601, 859
NlaIII	CATG	5 cut(s)	284, 520, 526, 571, 784
NsiI	ATGCAT	1 cut(s)	569
NspI	RCATGY	2 cut(s)	520, 571
PaeI	GCATGC	1 cut(s)	571
PceI	AGGCCT	1 cut(s)	686
PciI	ACATGT	1 cut(s)	516
PdmI	GAANNNNTTC	1 cut(s)	15
PfeI	GAWTC	1 cut(s)	197
PkrI	GCNGC	6 cut(s)	132, 255, 370, 402, 626, 660
PleI	GAGTC	2 cut(s)	100, 598
PpsI	GAGTC	2 cut(s)	100, 598
PscI	ACATGT	1 cut(s)	516
Psp6I	CCWGG	1 cut(s)	66
PspEI	GGTNACC	1 cut(s)	343
PspGI	CCWGG	1 cut(s)	66
RsaI	GTAC	4 cut(s)	89, 218, 608, 866
RsaNI	GTAC	4 cut(s)	88, 217, 607, 865
RseI	CAYNNNNRTG	1 cut(s)	521
SaqAI	TTAA	1 cut(s)	396
SatI	GCNGC	6 cut(s)	131, 254, 369, 401, 625, 659
Sau3AI	GATC	4 cut(s)	171, 262, 601, 859
SchI	GAGTC	2 cut(s)	101, 599
ScrFI	CCNGG	3 cut(s)	68, 97, 98
SduI	GDGCHC	2 cut(s)	125, 280
SfaNI	GCATC	1 cut(s)	554
SfcI	CTRYAG	2 cut(s)	481, 739
SmaI	CCCGGG	1 cut(s)	98
SmiMI	CAYNNNNRTG	1 cut(s)	521
SmlI	CTYRAG	2 cut(s)	109, 134
SmoI	CTYRAG	2 cut(s)	109, 134
SphI	GCATGC	1 cut(s)	571
Sse9I	AATT	1 cut(s)	612
SseBI	AGGCCT	1 cut(s)	686
SsiI	CCGC	1 cut(s)	71
SspMI	CTAG	5 cut(s)	273, 416, 455, 674, 713
StuI	AGGCCT	1 cut(s)	686
StyD4I	CCNGG	3 cut(s)	66, 95, 96
TaiI	ACGT	2 cut(s)	465, 723
TaqI	TCGA	1 cut(s)	84
TaqII	GACCGA	1 cut(s)	202
TasI	AATT	1 cut(s)	612
TatI	WGTACW	3 cut(s)	216, 606, 864
TfiI	GAWTC	1 cut(s)	197
Tru1I	TTAA	1 cut(s)	396
Tru9I	TTAA	1 cut(s)	396
TscAI	CASTG	1 cut(s)	762
TseI	GCWGC	6 cut(s)	130, 253, 368, 400, 624, 658
TspDTI	ATGAA	2 cut(s)	155, 902
TspMI	CCCGGG	1 cut(s)	96
TspRI	CASTG	1 cut(s)	762
XapI	RAATTY	1 cut(s)	612
XceI	RCATGY	2 cut(s)	520, 571
XmaI	CCCGGG	1 cut(s)	96
XmnI	GAANNNNTTC	1 cut(s)	15
XspI	CTAG	5 cut(s)	273, 416, 455, 674, 713
Zsp2I	ATGCAT	1 cut(s)	569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.