FvH4_3g16230

glutathione transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000304
Physical Location & Seq
Forward (+)
10234244 .. 10235587
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16230.t1

Sequence Viewer

Length: 663 bp
ATGGCGGAGGAGGTTGTTGTTCTGGGCTTGTGGTCGAGCATGATCGCTATGAGAACCAGAGTTGCTCTGGCCGAGAAAGGGGTGGAGTATGAGTACAGAGAGGAGGACTTGCGCAACAAAACCTCACTGCTTCTGGAGATGAACCCGATCCACAAGAAGATCCCGGTTCTTATCCACAACGGCAAACCGGTCTGTGAGTCAGCCAACATCGTCCAGTACATCGATGAGGCTTGGAAGGACAAAGGGCCTGCTTTGCTTCCCTCCGATCCTTACGAGAGAGCTCAGGCCAGGTTCTGGGTTGATTACATTGATAAAAACCTATATGAAGCTGGGAAGAATATATGGTCCACAAAGGGAGAAGAACAAGAGGCAGCCAAGAAGATTTTTTTGGAAATCCTTAAGCTGCTGGAGGGAGAACTTGGGGACAAGACGTACTTTGGGGGAGAGAGCTTCGGGTTTGTAGACATCAATCTTGTCACATTTCACTGCTGGTTTTTTACCTATGAGACCATCGGAAACTTCAGCATAGAGGCAGTTTGCCCCACACTGATTGCATGGTGCAAAAGGTGTATGCAGAAGGAGAGAGTCTCCAGGATTCTTCCAGACCCAAGAAAGGTGTATGATAAGTGCCTTTTCTTACAGAAGTATTATGGTGTGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_N

Protein Analysis

221

Amino Acids

25.49

Weight (kDa)

5.77

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 6 - 76 3.6e-19 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 13 - 77 1.3e-12 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 15 - 76 1e-12 Glutathione S-transferase, N-terminal domain
GST_C PF00043 117 - 193 8.9e-10 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 122 - 189 5.9e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 113
AccB7I CCANNNNNTGG 1 cut(s) 294
AccI GTMKAC 1 cut(s) 462
AciI CCGC 1 cut(s) 5
AclWI GGATC 3 cut(s) 142, 154, 260
AcoI YGGCCR 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 505
AfaI GTAC 3 cut(s) 95, 218, 434
AfiI CCNNNNNNNGG 3 cut(s) 78, 294, 613
AflII CTTAAG 1 cut(s) 398
AgeI ACCGGT 1 cut(s) 187
AjnI CCWGG 2 cut(s) 287, 590
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 4 cut(s) 281, 329, 403, 450
AluI AGCT 4 cut(s) 281, 329, 403, 450
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 2 cut(s) 500, 592
AlwI GGATC 3 cut(s) 142, 154, 260
AlwNI CAGNNNCTG 1 cut(s) 294
AoxI GGCC 3 cut(s) 69, 245, 285
ApeKI GCWGC 2 cut(s) 371, 403
AsiGI ACCGGT 1 cut(s) 187
AspLEI GCGC 1 cut(s) 114
AspS9I GGNCC 2 cut(s) 245, 345
AsuC2I CCSGG 1 cut(s) 164
AvaII GGWCC 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 660
BanII GRGCYC 1 cut(s) 283
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 2 cut(s) 383, 390
BccI CCATC 1 cut(s) 518
BceAI ACGGC 1 cut(s) 196
BciT130I CCWGG 2 cut(s) 289, 592
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 2 cut(s) 500, 592
BfaI CTAG 1 cut(s) 661
BfrI CTTAAG 1 cut(s) 398
BisI GCNGC 2 cut(s) 372, 404
BlsI GCNGC 2 cut(s) 373, 405
Bme1390I CCNGG 3 cut(s) 164, 289, 592
Bme18I GGWCC 1 cut(s) 345
BmgT120I GGNCC 2 cut(s) 245, 345
BmrFI CCNGG 3 cut(s) 164, 289, 592
BplI GAGNNNNNCTC 2 cut(s) 572, 604
BpmI CTGGAG 3 cut(s) 155, 428, 574
Bpu10I CCTNAGC 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 164
Bsa29I ATCGAT 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 500
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 294, 613
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 214
BseBI CCWGG 2 cut(s) 289, 592
BseCI ATCGAT 1 cut(s) 222
BseLI CCNNNNNNNGG 3 cut(s) 78, 294, 613
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 1 cut(s) 214
BseRI GAGGAG 2 cut(s) 23, 116
BseSI GKGCMC 1 cut(s) 660
BseXI GCAGC 2 cut(s) 383, 390
BseYI CCCAGC 1 cut(s) 329
BshFI GGCC 3 cut(s) 71, 247, 287
BshTI ACCGGT 1 cut(s) 187
BshVI ATCGAT 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 283
BsiSI CCGG 2 cut(s) 164, 188
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 3 cut(s) 78, 294, 613
BsmAI GTCTC 2 cut(s) 500, 592
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 3 cut(s) 71, 247, 287
Bso31I GGTCTC 1 cut(s) 500
Bsp1286I GDGCHC 2 cut(s) 283, 660
Bsp143I GATC 4 cut(s) 42, 147, 159, 265
BspACI CCGC 1 cut(s) 5
BspANI GGCC 3 cut(s) 71, 247, 287
BspCNI CTCAG 1 cut(s) 295
BspDI ATCGAT 1 cut(s) 222
BspPI GGATC 3 cut(s) 142, 154, 260
BspTI CTTAAG 1 cut(s) 398
BspTNI GGTCTC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 187
BssMI GATC 4 cut(s) 42, 147, 159, 265
Bst2UI CCWGG 2 cut(s) 289, 592
BstAFI CTTAAG 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 1 cut(s) 282
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 4 cut(s) 45, 150, 162, 268
BstMAI GTCTC 2 cut(s) 500, 592
BstMBI GATC 4 cut(s) 42, 147, 159, 265
BstMWI GCNNNNNNNGC 1 cut(s) 253
BstNI CCWGG 2 cut(s) 289, 592
BstSCI CCNGG 3 cut(s) 162, 287, 590
BstSLI GKGCMC 1 cut(s) 660
BstV1I GCAGC 2 cut(s) 383, 390
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
Bsu15I ATCGAT 1 cut(s) 222
BsuRI GGCC 3 cut(s) 71, 247, 287
BsuTUI ATCGAT 1 cut(s) 222
BtsI GCAGTG 2 cut(s) 125, 484
BtsIMutI CAGTG 3 cut(s) 125, 484, 545
Cac8I GCNNGC 1 cut(s) 249
CaiI CAGNNNCTG 1 cut(s) 294
CfoI GCGC 1 cut(s) 114
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 245, 345
ClaI ATCGAT 1 cut(s) 222
Csp6I GTAC 3 cut(s) 94, 217, 433
CspAI ACCGGT 1 cut(s) 187
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CviAII CATG 2 cut(s) 40, 555
CviQI GTAC 3 cut(s) 94, 217, 433
DdeI CTNAG 1 cut(s) 282
DpnI GATC 4 cut(s) 44, 149, 161, 267
DpnII GATC 4 cut(s) 42, 147, 159, 265
EaeI YGGCCR 1 cut(s) 69
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 345
Eco53kI GAGCTC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 505
EcoICRI GAGCTC 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 287, 590
EcoT38I GRGCYC 1 cut(s) 283
FaeI CATG 2 cut(s) 43, 558
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 1 cut(s) 437
FatI CATG 2 cut(s) 39, 554
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 2 cut(s) 372, 404
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 2 cut(s) 372, 404
FspBI CTAG 1 cut(s) 661
FspI TGCGCA 1 cut(s) 113
GlaI GCGC 1 cut(s) 113
GluI GCNGC 2 cut(s) 372, 404
GsaI CCCAGC 1 cut(s) 333
GsuI CTGGAG 3 cut(s) 155, 428, 574
HaeIII GGCC 3 cut(s) 71, 247, 287
HapII CCGG 2 cut(s) 164, 188
HhaI GCGC 1 cut(s) 114
Hin1II CATG 2 cut(s) 43, 558
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HinfI GANTC 3 cut(s) 197, 585, 595
HpaII CCGG 2 cut(s) 164, 188
Hpy166II GTNNAC 2 cut(s) 348, 463
Hpy188I TCNGA 2 cut(s) 265, 515
Hpy188III TCNNGA 2 cut(s) 134, 602
Hpy8I GTNNAC 2 cut(s) 348, 463
HpyAV CCTTC 2 cut(s) 229, 571
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 3 cut(s) 554, 561, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 253
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 1 cut(s) 431
Hsp92II CATG 2 cut(s) 43, 558
HspAI GCGC 1 cut(s) 112
Kzo9I GATC 4 cut(s) 42, 147, 159, 265
Lsp1109I GCAGC 2 cut(s) 383, 390
MaeI CTAG 1 cut(s) 661
MaeII ACGT 1 cut(s) 431
MaeIII GTNAC 1 cut(s) 475
MalI GATC 4 cut(s) 44, 149, 161, 267
MboI GATC 4 cut(s) 42, 147, 159, 265
MboII GAAGA 5 cut(s) 169, 346, 371, 391, 590
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 283, 660
MlyI GAGTC 2 cut(s) 206, 594
MnlI CCTC 9 cut(s) 4, 94, 97, 133, 220, 271, 361, 403, 523
MseI TTAA 1 cut(s) 399
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 2 cut(s) 164, 188
MspR9I CCNGG 3 cut(s) 164, 289, 592
MvaI CCWGG 2 cut(s) 289, 592
MwoI GCNNNNNNNGC 1 cut(s) 253
NciI CCSGG 1 cut(s) 164
NdeII GATC 4 cut(s) 42, 147, 159, 265
NlaIII CATG 2 cut(s) 43, 558
NmeAIII GCCGAG 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 475
NsbI TGCGCA 1 cut(s) 113
PfeI GAWTC 1 cut(s) 595
PflMI CCANNNNNTGG 1 cut(s) 294
PfoI TCCNGGA 1 cut(s) 590
PinAI ACCGGT 1 cut(s) 187
PkrI GCNGC 2 cut(s) 373, 405
PleI GAGTC 2 cut(s) 205, 593
PpsI GAGTC 2 cut(s) 205, 593
Psp124BI GAGCTC 1 cut(s) 283
Psp6I CCWGG 2 cut(s) 287, 590
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 2 cut(s) 287, 590
PspPI GGNCC 2 cut(s) 245, 345
PstNI CAGNNNCTG 1 cut(s) 294
PsuI RGATCY 1 cut(s) 159
RsaI GTAC 3 cut(s) 95, 218, 434
RsaNI GTAC 3 cut(s) 94, 217, 433
SacI GAGCTC 1 cut(s) 283
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 2 cut(s) 372, 404
Sau3AI GATC 4 cut(s) 42, 147, 159, 265
Sau96I GGNCC 2 cut(s) 245, 345
SchI GAGTC 2 cut(s) 206, 594
ScrFI CCNGG 3 cut(s) 164, 289, 592
SduI GDGCHC 2 cut(s) 283, 660
SinI GGWCC 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 661
SstI GAGCTC 1 cut(s) 283
StyD4I CCNGG 3 cut(s) 162, 287, 590
TaiI ACGT 1 cut(s) 434
TaqI TCGA 2 cut(s) 35, 222
TatI WGTACW 2 cut(s) 93, 216
TfiI GAWTC 1 cut(s) 595
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 3 cut(s) 132, 491, 552
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 2 cut(s) 371, 403
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 2 cut(s) 155, 339
TspRI CASTG 3 cut(s) 132, 491, 552
Van91I CCANNNNNTGG 1 cut(s) 294
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 462
XspI CTAG 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.