Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000304

Physical Location & Seq

Genomic Coordinates Forward (+)

34198838 .. 34200200

Gene Structure 1363 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1210600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 657 bp

ATGGCGGATGAGGTGGTGTTGCTGGATTTCTGGCCAAGCCCATTTGGGATGAGACTGAGGATTGCTCTGGCTGAGAAGGGCATTGTGTATGAGTACAAAGATGAGGATATTTGGAACAAGAGCCCACTGTTGCTGCAGATGAACCCGGTTCACAAGAAGATCCCGGTTCTCATTCACAATGGAAAGCCCGTCTGTGAGTCCCTCATTGCGCTTCAGTACATTGATGAGGTTTGGAATGATAAGGCTCCGCTCTTGCCCTCCGACTCTTACCTCAGAGCCCAGGCCAGGTTCTGGGCTGACTTCGTCGACAAGAAGATATATGAGATTGGGAGGAAGCTGTGGACAACCAAAGGAGAAGAACAGGATGCAGTAAAAAAAGAATTTCTCGACTGCATTGGCGTGTTAGAAAGAGAGCTTGGAGACAAGCCTTTCTTTGGGGGGAATACCCTCGGATTCGTGGACGTGGCGCTTATTCCATACTATAGCTGGTTTCTTGTGTATGAGAAATTTGGCAACTTTAGTGTTGAGGCAGAGCACCCAGAATTTATTGCCTGGGTTAAGAGGTGCATGGAGAAGAAGAGCGTGTCCAAGTCGCTTCCCGAGCAGGAAAAGGTCTACGATTTTGTTATGCTTGTAAGGAAAAAGCATGGAATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009404 GO:0009407 GO:0009636 GO:0009987 GO:0016740 GO:0016765 GO:0019748 GO:0034641 GO:0042221 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044464 GO:0050896 GO:0051186 GO:0071704 GO:0098754 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

219

Amino Acids

25.4

Weight (kDa)

5.89

Isoelectric Point (pI)

31.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	5 - 76	8.2e-19	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	12 - 76	2.4e-11	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	13 - 76	1.9e-13	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	117 - 191	2.5e-08	Glutathione S-transferase, C-terminal domain
GST_C_2	PF13410	121 - 188	1e-09	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000304)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca	FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica	MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica	Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis	pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis	RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata	RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora	Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii	Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa	Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha	Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana	Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	291
AccBSI	CCGCTC	1 cut(s)	250
AccI	GTMKAC	2 cut(s)	306, 615
AciI	CCGC	2 cut(s)	5, 248
AclWI	GGATC	1 cut(s)	154
AcoI	YGGCCR	1 cut(s)	32
AcsI	RAATTY	4 cut(s)	380, 506, 542, 651
AcuI	CTGAAG	1 cut(s)	197
AfaI	GTAC	2 cut(s)	95, 218
AfiI	CCNNNNNNNGG	3 cut(s)	285, 291, 434
AjiI	CACGTC	1 cut(s)	463
AjnI	CCWGG	3 cut(s)	279, 284, 551
AjuI	GAANNNNNNNTTGG	2 cut(s)	399, 431
AluBI	AGCT	3 cut(s)	337, 415, 486
AluI	AGCT	3 cut(s)	337, 415, 486
Alw21I	GWGCWC	1 cut(s)	537
Alw26I	GTCTC	2 cut(s)	46, 414
AlwI	GGATC	1 cut(s)	154
AlwNI	CAGNNNCTG	1 cut(s)	291
Ama87I	CYCGRG	1 cut(s)	599
AoxI	GGCC	2 cut(s)	32, 282
ApeKI	GCWGC	1 cut(s)	133
ApoI	RAATTY	4 cut(s)	380, 506, 542, 651
AspLEI	GCGC	2 cut(s)	211, 469
AsuC2I	CCSGG	2 cut(s)	146, 164
AvaI	CYCGRG	1 cut(s)	599
BalI	TGGCCA	1 cut(s)	34
BanII	GRGCYC	2 cut(s)	125, 280
Bbv12I	GWGCWC	1 cut(s)	537
BbvI	GCAGC	1 cut(s)	120
BciT130I	CCWGG	3 cut(s)	281, 286, 553
BcnI	CCSGG	2 cut(s)	146, 164
BcoDI	GTCTC	2 cut(s)	46, 414
BfmI	CTRYAG	2 cut(s)	134, 481
BfoI	RGCGCY	1 cut(s)	470
BisI	GCNGC	1 cut(s)	134
BlsI	GCNGC	1 cut(s)	135
Bme1390I	CCNGG	5 cut(s)	146, 164, 281, 286, 553
BmeT110I	CYCGRG	1 cut(s)	599
BmgBI	CACGTC	1 cut(s)	463
BmiI	GGNNCC	1 cut(s)	246
BmrFI	CCNGG	5 cut(s)	146, 164, 281, 286, 553
BmsI	GCATC	1 cut(s)	355
BplI	GAGNNNNNCTC	2 cut(s)	49, 81
BpuMI	CCSGG	2 cut(s)	146, 164
BsaJI	CCNNGG	3 cut(s)	279, 448, 552
Bsc4I	CCNNNNNNNGG	3 cut(s)	285, 291, 434
Bse3DI	GCAATG	1 cut(s)	204
BseBI	CCWGG	3 cut(s)	281, 286, 553
BseDI	CCNNGG	3 cut(s)	279, 448, 552
BseGI	GGATG	3 cut(s)	13, 54, 370
BseLI	CCNNNNNNNGG	3 cut(s)	285, 291, 434
BseMI	GCAATG	1 cut(s)	204
BseMII	CTCAG	3 cut(s)	47, 63, 286
BseXI	GCAGC	1 cut(s)	120
BshFI	GGCC	2 cut(s)	34, 284
BsiHKAI	GWGCWC	1 cut(s)	537
BsiHKCI	CYCGRG	1 cut(s)	599
BsiSI	CCGG	2 cut(s)	146, 164
BslFI	GGGAC	1 cut(s)	184
BslI	CCNNNNNNNGG	3 cut(s)	285, 291, 434
BsmAI	GTCTC	2 cut(s)	46, 414
BsmFI	GGGAC	1 cut(s)	184
BsnI	GGCC	2 cut(s)	34, 284
BsoBI	CYCGRG	1 cut(s)	599
Bsp1286I	GDGCHC	3 cut(s)	125, 280, 537
Bsp143I	GATC	1 cut(s)	159
BspACI	CCGC	2 cut(s)	5, 248
BspANI	GGCC	2 cut(s)	34, 284
BspCNI	CTCAG	3 cut(s)	48, 64, 285
BspLI	GGNNCC	1 cut(s)	246
BspMAI	CTGCAG	1 cut(s)	138
BspPI	GGATC	1 cut(s)	154
BspQI	GCTCTTC	1 cut(s)	572
BsrBI	CCGCTC	1 cut(s)	250
BsrDI	GCAATG	1 cut(s)	204
BssECI	CCNNGG	3 cut(s)	279, 448, 552
BssMI	GATC	1 cut(s)	159
Bst2UI	CCWGG	3 cut(s)	281, 286, 553
Bst4CI	ACNGT	1 cut(s)	129
Bst6I	CTCTTC	1 cut(s)	572
BstDEI	CTNAG	3 cut(s)	56, 72, 272
BstF5I	GGATG	3 cut(s)	13, 54, 370
BstH2I	RGCGCY	1 cut(s)	470
BstHHI	GCGC	2 cut(s)	211, 469
BstKTI	GATC	1 cut(s)	162
BstMAI	GTCTC	2 cut(s)	46, 414
BstMBI	GATC	1 cut(s)	159
BstMWI	GCNNNNNNNGC	1 cut(s)	601
BstNI	CCWGG	3 cut(s)	281, 286, 553
BstSCI	CCNGG	5 cut(s)	144, 162, 279, 284, 551
BstSFI	CTRYAG	2 cut(s)	134, 481
BstV1I	GCAGC	1 cut(s)	120
BstX2I	RGATCY	1 cut(s)	159
BstYI	RGATCY	1 cut(s)	159
BsuRI	GGCC	2 cut(s)	34, 284
BtrI	CACGTC	1 cut(s)	463
BtsCI	GGATG	3 cut(s)	13, 54, 370
BtsIMutI	CAGTG	1 cut(s)	125
CaiI	CAGNNNCTG	1 cut(s)	291
CfoI	GCGC	2 cut(s)	211, 469
Csp6I	GTAC	2 cut(s)	94, 217
CviAII	CATG	2 cut(s)	568, 647
CviQI	GTAC	2 cut(s)	94, 217
DdeI	CTNAG	3 cut(s)	56, 72, 272
DpnI	GATC	1 cut(s)	161
DpnII	GATC	1 cut(s)	159
EaeI	YGGCCR	1 cut(s)	32
Eam1104I	CTCTTC	1 cut(s)	572
EarI	CTCTTC	1 cut(s)	572
EciI	GGCGGA	1 cut(s)	20
Eco24I	GRGCYC	2 cut(s)	125, 280
Eco57I	CTGAAG	1 cut(s)	197
Eco88I	CYCGRG	1 cut(s)	599
EcoRII	CCWGG	3 cut(s)	279, 284, 551
EcoT38I	GRGCYC	2 cut(s)	125, 280
FaeI	CATG	2 cut(s)	571, 650
FaiI	YATR	9 cut(s)	90, 319, 321, 478, 483, 501, 569, 629, 648
FalI	AAGNNNNNCTT	2 cut(s)	416, 448
FaqI	GGGAC	1 cut(s)	184
FatI	CATG	2 cut(s)	567, 646
FblI	GTMKAC	2 cut(s)	306, 615
Fnu4HI	GCNGC	1 cut(s)	134
FokI	GGATG	3 cut(s)	20, 61, 377
FriOI	GRGCYC	2 cut(s)	125, 280
Fsp4HI	GCNGC	1 cut(s)	134
GlaI	GCGC	2 cut(s)	210, 468
GluI	GCNGC	1 cut(s)	134
HaeII	RGCGCY	1 cut(s)	470
HaeIII	GGCC	2 cut(s)	34, 284
HapII	CCGG	2 cut(s)	146, 164
HhaI	GCGC	2 cut(s)	211, 469
Hin1II	CATG	2 cut(s)	571, 650
Hin6I	GCGC	2 cut(s)	209, 467
HinP1I	GCGC	2 cut(s)	209, 467
HincII	GTYRAC	1 cut(s)	307
HindII	GTYRAC	1 cut(s)	307
HinfI	GANTC	3 cut(s)	197, 263, 453
HpaII	CCGG	2 cut(s)	146, 164
Hpy166II	GTNNAC	5 cut(s)	151, 307, 342, 460, 616
Hpy188I	TCNGA	3 cut(s)	262, 275, 452
Hpy188III	TCNNGA	2 cut(s)	386, 599
Hpy8I	GTNNAC	5 cut(s)	151, 307, 342, 460, 616
Hpy99I	CGWCG	1 cut(s)	308
HpyAV	CCTTC	1 cut(s)	70
HpyCH4III	ACNGT	1 cut(s)	129
HpyCH4IV	ACGT	1 cut(s)	462
HpyCH4V	TGCA	4 cut(s)	136, 368, 393, 567
HpyF10VI	GCNNNNNNNGC	1 cut(s)	601
HpyF3I	CTNAG	3 cut(s)	56, 72, 272
HpySE526I	ACGT	1 cut(s)	462
Hsp92II	CATG	2 cut(s)	571, 650
HspAI	GCGC	2 cut(s)	209, 467
Kzo9I	GATC	1 cut(s)	159
LguI	GCTCTTC	1 cut(s)	572
LmnI	GCTCC	1 cut(s)	250
Lsp1109I	GCAGC	1 cut(s)	120
LweI	GCATC	1 cut(s)	355
MaeII	ACGT	1 cut(s)	462
MalI	GATC	1 cut(s)	161
MbiI	CCGCTC	1 cut(s)	250
MboI	GATC	1 cut(s)	159
MboII	GAAGA	5 cut(s)	169, 325, 368, 586, 589
MflI	RGATCY	1 cut(s)	159
MhlI	GDGCHC	3 cut(s)	125, 280, 537
MlsI	TGGCCA	1 cut(s)	34
MluCI	AATT	4 cut(s)	380, 506, 542, 651
MluNI	TGGCCA	1 cut(s)	34
MlyI	GAGTC	2 cut(s)	206, 257
MmeI	TCCRAC	1 cut(s)	285
Mox20I	TGGCCA	1 cut(s)	34
MscI	TGGCCA	1 cut(s)	34
MseI	TTAA	1 cut(s)	558
MslI	CAYNNNNRTG	1 cut(s)	398
Msp20I	TGGCCA	1 cut(s)	34
MspI	CCGG	2 cut(s)	146, 164
MspR9I	CCNGG	5 cut(s)	146, 164, 281, 286, 553
MvaI	CCWGG	3 cut(s)	281, 286, 553
MwoI	GCNNNNNNNGC	1 cut(s)	601
NciI	CCSGG	2 cut(s)	146, 164
NdeII	GATC	1 cut(s)	159
NlaIII	CATG	2 cut(s)	571, 650
NlaIV	GGNNCC	1 cut(s)	246
PciSI	GCTCTTC	1 cut(s)	572
PfeI	GAWTC	1 cut(s)	453
PflFI	GACNNNGTC	1 cut(s)	302
PflMI	CCANNNNNTGG	1 cut(s)	291
PkrI	GCNGC	1 cut(s)	135
PleI	GAGTC	2 cut(s)	205, 257
PpsI	GAGTC	2 cut(s)	205, 257
Psp6I	CCWGG	3 cut(s)	279, 284, 551
PspGI	CCWGG	3 cut(s)	279, 284, 551
PspN4I	GGNNCC	1 cut(s)	246
PstI	CTGCAG	1 cut(s)	138
PstNI	CAGNNNCTG	1 cut(s)	291
PsuI	RGATCY	1 cut(s)	159
PsyI	GACNNNGTC	1 cut(s)	302
RsaI	GTAC	2 cut(s)	95, 218
RsaNI	GTAC	2 cut(s)	94, 217
RseI	CAYNNNNRTG	1 cut(s)	398
SalI	GTCGAC	1 cut(s)	305
SapI	GCTCTTC	1 cut(s)	572
SaqAI	TTAA	1 cut(s)	558
SatI	GCNGC	1 cut(s)	134
Sau3AI	GATC	1 cut(s)	159
SchI	GAGTC	2 cut(s)	206, 257
ScrFI	CCNGG	5 cut(s)	146, 164, 281, 286, 553
SduI	GDGCHC	3 cut(s)	125, 280, 537
SfaNI	GCATC	1 cut(s)	355
SfcI	CTRYAG	2 cut(s)	134, 481
SmiMI	CAYNNNNRTG	1 cut(s)	398
Sse9I	AATT	4 cut(s)	380, 506, 542, 651
SsiI	CCGC	2 cut(s)	5, 248
StyD4I	CCNGG	5 cut(s)	144, 162, 279, 284, 551
TaaI	ACNGT	1 cut(s)	129
TaiI	ACGT	1 cut(s)	465
TaqI	TCGA	2 cut(s)	306, 387
TasI	AATT	4 cut(s)	380, 506, 542, 651
TatI	WGTACW	2 cut(s)	93, 216
TfiI	GAWTC	1 cut(s)	453
Tru1I	TTAA	1 cut(s)	558
Tru9I	TTAA	1 cut(s)	558
TscAI	CASTG	1 cut(s)	132
TseI	GCWGC	1 cut(s)	133
TspDTI	ATGAA	1 cut(s)	155
TspRI	CASTG	1 cut(s)	132
Tth111I	GACNNNGTC	1 cut(s)	302
Van91I	CCANNNNNTGG	1 cut(s)	291
XapI	RAATTY	4 cut(s)	380, 506, 542, 651
XcmI	CCANNNNNNNNNTGG	1 cut(s)	483
XmiI	GTMKAC	2 cut(s)	306, 615

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.