MD05G1210400.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000304
Physical Location & Seq
Forward (+)
34178564 .. 34182130
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1210400.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGCGGATGAGGTGGTTTTGTTGGATTCCTGGCAAAGCCCATTTGGGATGAGGCTAAGGATTGCTCTGGCTGAGAAGGGCATCGAGTATGAGTACAAAGATGAGGACTTGTGGAACAAGAGCCCACTGTTGCTGCAGATGAACCCGGTTCACAAGAAGATCCCGGTTCTCATTCACAATGAAAAACCGATCTGTGAGTCCCTATTTGCACTTCAGTACATTGATGAGGTTTGGAATGATAGGGCTCCACTCTTGCCCTCTGACTCTTACCTCAGAGCCCAGGCCAGATTCTGGGCTGACTTCGTCGACAAGAAGAAGATATTTCAGATTGGGAAGAAGGTGTGGACAACCAAAGGAGAAAAACAGGATGCAGCAAAAAAAAAATTTCTCGACTGCATAGGCGTGTTAGAAGGAGAGCTTGGAGACAAGCCTTTCTTTGGGGGGGATACCCTCGGATTCGTGGACGTGACCCTTATTCCAATCTATAGCTGGTTTCTTGTGTGTGAGAAATTTGGCGACTTCAGTGTTGAGGCTGAGCACCCAAAATTTATTGCCTGGGTTAAGAGGTGCATGGAGAAGGAGAGCGTGTCCAAGTCGCTTCCCGAGCAGGAAAAGGTCTATGATTACGCCATGCTTATCAGGAAAAAGCTTCGAATTGAGTAG

Protein Analysis

221

Amino Acids

25.61

Weight (kDa)

6.03

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 6 - 76 2.1e-19 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 12 - 76 2e-11 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 2.4e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 118 - 193 1.2e-08 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 123 - 189 3.1e-10 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 291
AccI GTMKAC 1 cut(s) 306
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 154
AcsI RAATTY 3 cut(s) 383, 509, 545
AcuI CTGAAG 2 cut(s) 197, 505
AfaI GTAC 2 cut(s) 95, 218
AfiI CCNNNNNNNGG 2 cut(s) 291, 437
AjiI CACGTC 1 cut(s) 466
AjnI CCWGG 3 cut(s) 29, 279, 554
AjuI GAANNNNNNNTTGG 2 cut(s) 402, 434
AluBI AGCT 3 cut(s) 418, 489, 649
AluI AGCT 3 cut(s) 418, 489, 649
Alw21I GWGCWC 1 cut(s) 540
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 1 cut(s) 154
AlwNI CAGNNNCTG 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 602
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 2 cut(s) 133, 371
ApoI RAATTY 3 cut(s) 383, 509, 545
AsuC2I CCSGG 2 cut(s) 146, 164
AsuII TTCGAA 1 cut(s) 652
AvaI CYCGRG 1 cut(s) 602
BanII GRGCYC 3 cut(s) 125, 247, 280
Bbv12I GWGCWC 1 cut(s) 540
BbvI GCAGC 2 cut(s) 120, 383
BciT130I CCWGG 3 cut(s) 31, 281, 556
BciVI GTATCC 1 cut(s) 439
BcnI CCSGG 2 cut(s) 146, 164
BcoDI GTCTC 1 cut(s) 417
BfmI CTRYAG 2 cut(s) 134, 484
BfuI GTATCC 1 cut(s) 439
BisI GCNGC 2 cut(s) 134, 372
BlpI GCTNAGC 1 cut(s) 534
BlsI GCNGC 2 cut(s) 135, 373
Bme1390I CCNGG 5 cut(s) 31, 146, 164, 281, 556
BmeT110I CYCGRG 1 cut(s) 602
BmgBI CACGTC 1 cut(s) 466
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 5 cut(s) 31, 146, 164, 281, 556
BmsI GCATC 2 cut(s) 90, 358
Bpu10I CCTNAGC 1 cut(s) 56
Bpu1102I GCTNAGC 1 cut(s) 534
Bpu14I TTCGAA 1 cut(s) 652
BpuMI CCSGG 2 cut(s) 146, 164
BsaJI CCNNGG 3 cut(s) 279, 451, 555
BsaXI ACNNNNNCTCC 2 cut(s) 572, 602
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 437
BseBI CCWGG 3 cut(s) 31, 281, 556
BseDI CCNNGG 3 cut(s) 279, 451, 555
BseGI GGATG 3 cut(s) 13, 54, 373
BseLI CCNNNNNNNGG 2 cut(s) 291, 437
BseMII CTCAG 3 cut(s) 63, 286, 525
BseXI GCAGC 2 cut(s) 120, 383
BshFI GGCC 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 540
BsiHKCI CYCGRG 1 cut(s) 602
BsiSI CCGG 2 cut(s) 146, 164
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 291, 437
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 602
Bsp119I TTCGAA 1 cut(s) 652
Bsp1286I GDGCHC 4 cut(s) 125, 247, 280, 540
Bsp143I GATC 2 cut(s) 159, 189
Bsp1720I GCTNAGC 1 cut(s) 534
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 3 cut(s) 64, 285, 526
BspLI GGNNCC 1 cut(s) 246
BspMAI CTGCAG 1 cut(s) 138
BspPI GGATC 1 cut(s) 154
BspT104I TTCGAA 1 cut(s) 652
BssECI CCNNGG 3 cut(s) 279, 451, 555
BssMI GATC 2 cut(s) 159, 189
Bst2UI CCWGG 3 cut(s) 31, 281, 556
Bst4CI ACNGT 1 cut(s) 129
BstBI TTCGAA 1 cut(s) 652
BstDEI CTNAG 4 cut(s) 56, 72, 272, 534
BstF5I GGATG 3 cut(s) 13, 54, 373
BstKTI GATC 2 cut(s) 162, 192
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 2 cut(s) 159, 189
BstMWI GCNNNNNNNGC 1 cut(s) 604
BstNI CCWGG 3 cut(s) 31, 281, 556
BstSCI CCNGG 5 cut(s) 29, 144, 162, 279, 554
BstSFI CTRYAG 2 cut(s) 134, 484
BstV1I GCAGC 2 cut(s) 120, 383
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuI GTATCC 1 cut(s) 439
BsuRI GGCC 1 cut(s) 284
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 3 cut(s) 13, 54, 373
BtsIMutI CAGTG 2 cut(s) 125, 529
CaiI CAGNNNCTG 1 cut(s) 291
Csp6I GTAC 2 cut(s) 94, 217
CviAII CATG 2 cut(s) 571, 631
CviQI GTAC 2 cut(s) 94, 217
DdeI CTNAG 4 cut(s) 56, 72, 272, 534
DpnI GATC 2 cut(s) 161, 191
DpnII GATC 2 cut(s) 159, 189
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 3 cut(s) 125, 247, 280
Eco57I CTGAAG 2 cut(s) 197, 505
Eco88I CYCGRG 1 cut(s) 602
EcoRII CCWGG 3 cut(s) 29, 279, 554
EcoT38I GRGCYC 3 cut(s) 125, 247, 280
FaeI CATG 2 cut(s) 574, 634
FaiI YATR 6 cut(s) 90, 398, 486, 572, 621, 632
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 419, 451
FaqI GGGAC 1 cut(s) 184
FatI CATG 2 cut(s) 570, 630
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 2 cut(s) 134, 372
FokI GGATG 3 cut(s) 20, 61, 380
FriOI GRGCYC 3 cut(s) 125, 247, 280
Fsp4HI GCNGC 2 cut(s) 134, 372
GluI GCNGC 2 cut(s) 134, 372
HaeIII GGCC 1 cut(s) 284
HapII CCGG 2 cut(s) 146, 164
Hin1II CATG 2 cut(s) 574, 634
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 5 cut(s) 26, 197, 263, 288, 456
HpaII CCGG 2 cut(s) 146, 164
Hpy166II GTNNAC 4 cut(s) 151, 307, 345, 463
Hpy188I TCNGA 4 cut(s) 262, 275, 327, 455
Hpy188III TCNNGA 3 cut(s) 389, 602, 640
Hpy8I GTNNAC 4 cut(s) 151, 307, 345, 463
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 4 cut(s) 70, 331, 404, 571
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 5 cut(s) 136, 209, 371, 396, 570
HpyF10VI GCNNNNNNNGC 1 cut(s) 604
HpyF3I CTNAG 4 cut(s) 56, 72, 272, 534
HpySE526I ACGT 1 cut(s) 465
Hsp92II CATG 2 cut(s) 574, 634
Kzo9I GATC 2 cut(s) 159, 189
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 2 cut(s) 120, 383
LweI GCATC 2 cut(s) 90, 358
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 1 cut(s) 466
MalI GATC 2 cut(s) 161, 191
MboI GATC 2 cut(s) 159, 189
MboII GAAGA 4 cut(s) 169, 325, 328, 346
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 4 cut(s) 125, 247, 280, 540
MluCI AATT 4 cut(s) 383, 509, 545, 654
MlyI GAGTC 2 cut(s) 206, 257
MnlI CCTC 9 cut(s) 4, 45, 97, 220, 268, 281, 461, 523, 558
MseI TTAA 1 cut(s) 561
MslI CAYNNNNRTG 1 cut(s) 401
MspI CCGG 2 cut(s) 146, 164
MspR9I CCNGG 5 cut(s) 31, 146, 164, 281, 556
MvaI CCWGG 3 cut(s) 31, 281, 556
MwoI GCNNNNNNNGC 1 cut(s) 604
NciI CCSGG 2 cut(s) 146, 164
NdeII GATC 2 cut(s) 159, 189
NlaIII CATG 2 cut(s) 574, 634
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 1 cut(s) 466
NspV TTCGAA 1 cut(s) 652
PfeI GAWTC 3 cut(s) 26, 288, 456
PflFI GACNNNGTC 1 cut(s) 302
PflMI CCANNNNNTGG 1 cut(s) 291
PkrI GCNGC 2 cut(s) 135, 373
PleI GAGTC 2 cut(s) 205, 257
PpsI GAGTC 2 cut(s) 205, 257
Psp6I CCWGG 3 cut(s) 29, 279, 554
PspGI CCWGG 3 cut(s) 29, 279, 554
PspN4I GGNNCC 1 cut(s) 246
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 291
PsuI RGATCY 1 cut(s) 159
PsyI GACNNNGTC 1 cut(s) 302
RsaI GTAC 2 cut(s) 95, 218
RsaNI GTAC 2 cut(s) 94, 217
RseI CAYNNNNRTG 1 cut(s) 401
SalI GTCGAC 1 cut(s) 305
SaqAI TTAA 1 cut(s) 561
SatI GCNGC 2 cut(s) 134, 372
Sau3AI GATC 2 cut(s) 159, 189
SchI GAGTC 2 cut(s) 206, 257
ScrFI CCNGG 5 cut(s) 31, 146, 164, 281, 556
SduI GDGCHC 4 cut(s) 125, 247, 280, 540
SfaNI GCATC 2 cut(s) 90, 358
SfcI CTRYAG 2 cut(s) 134, 484
SfuI TTCGAA 1 cut(s) 652
SmiMI CAYNNNNRTG 1 cut(s) 401
Sse9I AATT 4 cut(s) 383, 509, 545, 654
SsiI CCGC 1 cut(s) 5
StyD4I CCNGG 5 cut(s) 29, 144, 162, 279, 554
TaaI ACNGT 1 cut(s) 129
TaiI ACGT 1 cut(s) 468
TaqI TCGA 4 cut(s) 84, 306, 390, 652
TasI AATT 4 cut(s) 383, 509, 545, 654
TatI WGTACW 2 cut(s) 93, 216
TfiI GAWTC 3 cut(s) 26, 288, 456
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TscAI CASTG 2 cut(s) 132, 529
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 2 cut(s) 133, 371
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 2 cut(s) 155, 195
TspRI CASTG 2 cut(s) 132, 529
Tth111I GACNNNGTC 1 cut(s) 302
Van91I CCANNNNNTGG 1 cut(s) 291
XapI RAATTY 3 cut(s) 383, 509, 545
XcmI CCANNNNNNNNNTGG 1 cut(s) 486
XmiI GTMKAC 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.