Rroxscaffold_4G00329130

glutathione s-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000304
Physical Location & Seq
Reverse (-)
62323631 .. 62325059
1429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329130.1

Sequence Viewer

Length: 705 bp
ATGTTTGCCATGAGACCCAGAGTTGCTCTGACCGAGAAAGGGGTGGAGTATGAGTACAGAGAGGAGGACTTGCCCAACAAAACCTCACTGCTTCTGGAGATGAACCCGACCCACAAGAAAGTCCCCGTTCTTATCCACAACGGCAAACCCGTCTGTGAGTCGGCCAACATTGTCCAGTATATTGACGAGGCTTGGAAGGACATAGGTCCTACTTTGCTTCCCTCTGATCCTTACCAGAGAGCTCAGGCCAGGTTCTGGGTTGATTACATTGATAAAAAGTTATATGAAGCTGGGAAGAATATATGGTCCACAAAGGAGAAGAACAAGAAGCAGCCAAGAAAGTTTTTGGAAATCCTTAAGCTGCTGGAGGTAGAACTTGGGGACAAGACATACTTTGGGGGAGAGAGCTTTGGGTTCGTAGACATCAATCTTGTCCCATTTTACTGCTGGTTTTTGACCTATGAGACCATCGGAAACTTCAGCATAGAGGCCGAGTGCCCCAAACTGATTGCATGGTTCAACAGGTGTATGCAGAAGGAGAGCGTCTCCAAAACTCTTCCTGACCCAAAAAAGGTGTATGAGTTTTGCCTTTTCTTGCAAAAAGTATTATGGTGTGGCATAGTGGAAGAGAAAAGGGCTTTGCTTAACCATGATGTTATGGCTACTTGCAAAAGCATCTTGATGTTATGGCCTTTTCTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016740 GO:0016765 GO:0018973 GO:0018974 GO:0019326 GO:0019439 GO:0019748 GO:0019899 GO:0031090 GO:0031668 GO:0033218 GO:0033554 GO:0034641 GO:0042221 GO:0042277 GO:0042537 GO:0042631 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046256 GO:0046260 GO:0046263 GO:0046686 GO:0048037 GO:0048046 GO:0048583 GO:0050789 GO:0050896 GO:0051186 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071944 GO:0072341 GO:0072490 GO:0072491 GO:0080167 GO:0098588 GO:0098754 GO:0098805 GO:0104004 GO:1900750 GO:1901360 GO:1901361 GO:1901564 GO:1901575 GO:1901681 GO:1901700 GO:1901701 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

234

Amino Acids

27.38

Weight (kDa)

7.52

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 2 - 63 3.9e-17 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 2 - 63 2.6e-13 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 2 - 64 4.7e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 105 - 179 1.5e-10 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 112 - 175 9.6e-08 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 255
AccI GTMKAC 1 cut(s) 420
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 1 cut(s) 162
AcuI CTGAAG 1 cut(s) 463
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 3 cut(s) 39, 255, 571
AflII CTTAAG 1 cut(s) 356
AgsI TTSAA 1 cut(s) 520
AjnI CCWGG 1 cut(s) 248
AluBI AGCT 4 cut(s) 242, 290, 361, 408
AluI AGCT 4 cut(s) 242, 290, 361, 408
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 3 cut(s) 7, 458, 550
AlwI GGATC 1 cut(s) 221
AlwNI CAGNNNCTG 1 cut(s) 255
AoxI GGCC 4 cut(s) 162, 246, 489, 689
ApeKI GCWGC 2 cut(s) 331, 361
AspS9I GGNCC 2 cut(s) 206, 306
AvaII GGWCC 2 cut(s) 206, 306
BaeGI GKGCMC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 2 cut(s) 343, 348
BccI CCATC 1 cut(s) 476
BceAI ACGGC 1 cut(s) 157
BciT130I CCWGG 1 cut(s) 250
BcoDI GTCTC 3 cut(s) 7, 458, 550
BfrI CTTAAG 1 cut(s) 356
BisI GCNGC 2 cut(s) 332, 362
BlsI GCNGC 2 cut(s) 333, 363
Bme1390I CCNGG 1 cut(s) 250
Bme18I GGWCC 2 cut(s) 206, 306
BmgT120I GGNCC 2 cut(s) 206, 306
BmrFI CCNGG 1 cut(s) 250
BmsI GCATC 1 cut(s) 684
BoxI GACNNNNGTC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 530, 562
BpmI CTGGAG 2 cut(s) 116, 386
Bpu10I CCTNAGC 1 cut(s) 243
BsaI GGTCTC 2 cut(s) 7, 458
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 255, 571
Bse1I ACTGG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 250
BseLI CCNNNNNNNGG 3 cut(s) 39, 255, 571
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 1 cut(s) 175
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 500
BseXI GCAGC 2 cut(s) 343, 348
BseYI CCCAGC 1 cut(s) 290
BshFI GGCC 4 cut(s) 164, 248, 491, 691
BsiHKAI GWGCWC 1 cut(s) 244
BslFI GGGAC 3 cut(s) 107, 395, 419
BslI CCNNNNNNNGG 3 cut(s) 39, 255, 571
BsmAI GTCTC 3 cut(s) 7, 458, 550
BsmBI CGTCTC 1 cut(s) 550
BsmFI GGGAC 3 cut(s) 107, 395, 419
BsnI GGCC 4 cut(s) 164, 248, 491, 691
Bso31I GGTCTC 2 cut(s) 7, 458
Bsp1286I GDGCHC 2 cut(s) 244, 500
Bsp143I GATC 1 cut(s) 226
BspANI GGCC 4 cut(s) 164, 248, 491, 691
BspCNI CTCAG 1 cut(s) 256
BspPI GGATC 1 cut(s) 221
BspTI CTTAAG 1 cut(s) 356
BspTNI GGTCTC 2 cut(s) 7, 458
BsrI ACTGG 1 cut(s) 175
BssMI GATC 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 250
Bst6I CTCTTC 2 cut(s) 561, 621
BstAFI CTTAAG 1 cut(s) 356
BstDEI CTNAG 1 cut(s) 243
BstKTI GATC 1 cut(s) 229
BstMAI GTCTC 3 cut(s) 7, 458, 550
BstMBI GATC 1 cut(s) 226
BstNI CCWGG 1 cut(s) 250
BstPAI GACNNNNGTC 1 cut(s) 204
BstSCI CCNGG 1 cut(s) 248
BstSLI GKGCMC 1 cut(s) 500
BstV1I GCAGC 2 cut(s) 343, 348
BsuRI GGCC 4 cut(s) 164, 248, 491, 691
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 1 cut(s) 86
CaiI CAGNNNCTG 1 cut(s) 255
Cfr13I GGNCC 2 cut(s) 206, 306
CseI GACGC 1 cut(s) 532
Csp6I GTAC 1 cut(s) 55
CviAII CATG 3 cut(s) 10, 513, 650
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 1 cut(s) 243
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 162
Eam1104I CTCTTC 2 cut(s) 561, 621
EarI CTCTTC 2 cut(s) 561, 621
Ecl136II GAGCTC 1 cut(s) 242
Eco24I GRGCYC 1 cut(s) 244
Eco31I GGTCTC 2 cut(s) 7, 458
Eco47I GGWCC 2 cut(s) 206, 306
Eco53kI GAGCTC 1 cut(s) 242
Eco57I CTGAAG 1 cut(s) 463
EcoICRI GAGCTC 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 248
EcoT38I GRGCYC 1 cut(s) 244
Esp3I CGTCTC 1 cut(s) 550
FaeI CATG 3 cut(s) 13, 516, 653
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 3 cut(s) 107, 395, 419
FatI CATG 3 cut(s) 9, 512, 649
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 332, 362
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 2 cut(s) 332, 362
GluI GCNGC 2 cut(s) 332, 362
GsaI CCCAGC 1 cut(s) 294
GsuI CTGGAG 2 cut(s) 116, 386
HaeIII GGCC 4 cut(s) 164, 248, 491, 691
HgaI GACGC 1 cut(s) 532
Hin1II CATG 3 cut(s) 13, 516, 653
HinfI GANTC 1 cut(s) 158
Hpy166II GTNNAC 2 cut(s) 309, 421
Hpy188I TCNGA 3 cut(s) 30, 226, 473
Hpy188III TCNNGA 3 cut(s) 95, 560, 679
Hpy8I GTNNAC 2 cut(s) 309, 421
HpyAV CCTTC 2 cut(s) 190, 529
HpyCH4V TGCA 4 cut(s) 512, 532, 598, 669
HpyF3I CTNAG 1 cut(s) 243
Hsp92II CATG 3 cut(s) 13, 516, 653
Kzo9I GATC 1 cut(s) 226
Lsp1109I GCAGC 2 cut(s) 343, 348
LweI GCATC 1 cut(s) 684
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 4 cut(s) 307, 331, 548, 638
MhlI GDGCHC 2 cut(s) 244, 500
MlyI GAGTC 1 cut(s) 167
MnlI CCTC 7 cut(s) 55, 58, 94, 181, 232, 361, 481
MseI TTAA 3 cut(s) 357, 645, 703
MslI CAYNNNNRTG 1 cut(s) 680
MspCI CTTAAG 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 250
MvaI CCWGG 1 cut(s) 250
NdeII GATC 1 cut(s) 226
NlaIII CATG 3 cut(s) 13, 516, 653
NmeAIII GCCGAG 1 cut(s) 517
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PflMI CCANNNNNTGG 1 cut(s) 255
PkrI GCNGC 2 cut(s) 333, 363
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PpuMI RGGWCCY 1 cut(s) 206
PshAI GACNNNNGTC 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 248
PspPI GGNCC 2 cut(s) 206, 306
PspPPI RGGWCCY 1 cut(s) 206
PstNI CAGNNNCTG 1 cut(s) 255
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 680
SacI GAGCTC 1 cut(s) 244
SaqAI TTAA 3 cut(s) 357, 645, 703
SatI GCNGC 2 cut(s) 332, 362
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 2 cut(s) 206, 306
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 250
SduI GDGCHC 2 cut(s) 244, 500
SfaNI GCATC 1 cut(s) 684
SinI GGWCC 2 cut(s) 206, 306
SmiMI CAYNNNNRTG 1 cut(s) 680
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SstI GAGCTC 1 cut(s) 244
StyD4I CCNGG 1 cut(s) 248
TaqII GACCGA 1 cut(s) 47
TatI WGTACW 1 cut(s) 54
Tru1I TTAA 3 cut(s) 357, 645, 703
Tru9I TTAA 3 cut(s) 357, 645, 703
TscAI CASTG 1 cut(s) 93
TseI GCWGC 2 cut(s) 331, 361
TspDTI ATGAA 2 cut(s) 116, 300
TspRI CASTG 1 cut(s) 93
Van91I CCANNNNNTGG 1 cut(s) 255
Vha464I CTTAAG 1 cut(s) 356
VpaK11BI GGWCC 2 cut(s) 206, 306
XcmI CCANNNNNNNNNTGG 1 cut(s) 444
XmiI GTMKAC 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.