Rw5G016960

glutathione s-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000304
Physical Location & Seq
Forward (+)
21728272 .. 21747890
19619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016960.1

Sequence Viewer

Length: 660 bp
ATGGCGGACGAGGTTATTCTATTGGACGGTTATGTGAGCATGTTTGGGATGAGGGTCAGAGTAGCTCTGGCCGAGAAGGGAATCAAGTATGAGTACAGAGAAGAAGACATGAGGAACAAGAGCCCACTGCTTCTTAAGATGAACCCGATCCACAAGAAGATTCCGGTGCTCATCCATAATGGCAAACCGGTCTGTGAGTCGCTCATCATCGTGCAGTATATTGATGAAGTATGGAAGGATAAGGCTCCTCTGCTTCCCTCTGATCCTTACCAGAGAGCCCAGGCCAGATTCTGGGCCGATTTCATTGATAAGAAGTTATATGATGCGGGCAAGAAGATATGGACTACAAAAGGAGAAGAGCAGGAGGCAGCAAATAAGGAATTCATTGAAGTCCTGAAGGTGTTGGAAGGAGAGCTTGGAGACAAGCCTTATTTCATGGGTGAGAGATTTGGGTTCCTGGACATTGCTCTCATCACATTCTACAGCTGGTTTCATGCTTATGAGACACTTGGAAACTTCAGTATAGAGGCCGAGTGCCCCAAGCTGATTTCATGGGCCAAGAGGTGCTTGCAGAAGGAGAGTGTTTCAAAATCTCTTGCTGACCAGAAAAAGGTTTATGAGTATCTTCTTGAGATGAAGAAGAGGCTTGGTGTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016740 GO:0016765 GO:0018973 GO:0018974 GO:0019326 GO:0019439 GO:0019748 GO:0019899 GO:0031090 GO:0031668 GO:0033218 GO:0033554 GO:0034641 GO:0042221 GO:0042277 GO:0042537 GO:0042631 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046256 GO:0046260 GO:0046263 GO:0046686 GO:0048037 GO:0048046 GO:0048583 GO:0050789 GO:0050896 GO:0051186 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071944 GO:0072341 GO:0072490 GO:0072491 GO:0080167 GO:0098588 GO:0098754 GO:0098805 GO:0104004 GO:1900750 GO:1901360 GO:1901361 GO:1901564 GO:1901575 GO:1901681 GO:1901700 GO:1901701 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

219

Amino Acids

25.51

Weight (kDa)

6.86

Isoelectric Point (pI)

30.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 4.1e-21 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 11 - 76 5.3e-16 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 6.6e-14 Glutathione S-transferase, N-terminal domain
GST_C PF00043 116 - 192 2.7e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 121 - 188 1.1e-10 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 291
AciI CCGC 2 cut(s) 5, 326
AclWI GGATC 2 cut(s) 142, 257
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 2 cut(s) 416, 502
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 2 cut(s) 291, 610
AflII CTTAAG 1 cut(s) 134
AgeI ACCGGT 1 cut(s) 187
AgsI TTSAA 2 cut(s) 389, 588
AjnI CCWGG 2 cut(s) 279, 456
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 4 cut(s) 65, 415, 486, 544
AluI AGCT 4 cut(s) 65, 415, 486, 544
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 2 cut(s) 414, 497
AlwI GGATC 2 cut(s) 142, 257
AlwNI CAGNNNCTG 1 cut(s) 291
AoxI GGCC 5 cut(s) 69, 282, 294, 528, 555
ApeKI GCWGC 1 cut(s) 368
ApoI RAATTY 1 cut(s) 380
AsiGI ACCGGT 1 cut(s) 187
AspS9I GGNCC 2 cut(s) 294, 555
AsuHPI GGTGA 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 539
BanII GRGCYC 2 cut(s) 125, 280
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 380
BciT130I CCWGG 2 cut(s) 281, 458
BcoDI GTCTC 2 cut(s) 414, 497
BfmI CTRYAG 1 cut(s) 481
BfrI CTTAAG 1 cut(s) 134
BisI GCNGC 1 cut(s) 369
BlsI GCNGC 1 cut(s) 370
Bme1390I CCNGG 2 cut(s) 281, 458
BmgT120I GGNCC 2 cut(s) 294, 555
BmiI GGNNCC 2 cut(s) 246, 455
BmrFI CCNGG 2 cut(s) 281, 458
BmsI GCATC 1 cut(s) 313
BpiI GAAGAC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 650
BsaJI CCNNGG 1 cut(s) 279
BsaWI WCCGGW 2 cut(s) 163, 187
Bsc4I CCNNNNNNNGG 2 cut(s) 291, 610
Bse118I RCCGGY 1 cut(s) 187
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 2 cut(s) 281, 458
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 2 cut(s) 54, 171
BseLI CCNNNNNNNGG 2 cut(s) 291, 610
BseMI GCAATG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 237
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 1 cut(s) 380
BsgI GTGCAG 1 cut(s) 233
BshFI GGCC 5 cut(s) 71, 284, 296, 530, 557
BshTI ACCGGT 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 2 cut(s) 164, 188
BslI CCNNNNNNNGG 2 cut(s) 291, 610
BsmAI GTCTC 2 cut(s) 414, 497
BsnI GGCC 5 cut(s) 71, 284, 296, 530, 557
Bsp1286I GDGCHC 4 cut(s) 125, 171, 280, 539
Bsp143I GATC 2 cut(s) 147, 262
BspACI CCGC 2 cut(s) 5, 326
BspANI GGCC 5 cut(s) 71, 284, 296, 530, 557
BspLI GGNNCC 2 cut(s) 246, 455
BspPI GGATC 2 cut(s) 142, 257
BspQI GCTCTTC 1 cut(s) 351
BspTI CTTAAG 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 2 cut(s) 147, 262
Bst2UI CCWGG 2 cut(s) 281, 458
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 2 cut(s) 351, 635
BstAFI CTTAAG 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 328, 569
BstF5I GGATG 2 cut(s) 54, 171
BstKTI GATC 2 cut(s) 150, 265
BstMAI GTCTC 2 cut(s) 414, 497
BstMBI GATC 2 cut(s) 147, 262
BstNI CCWGG 2 cut(s) 281, 458
BstNSI RCATGY 1 cut(s) 43
BstSCI CCNGG 2 cut(s) 279, 456
BstSFI CTRYAG 1 cut(s) 481
BstSLI GKGCMC 1 cut(s) 539
BstV1I GCAGC 1 cut(s) 380
BstV2I GAAGAC 1 cut(s) 111
BsuRI GGCC 5 cut(s) 71, 284, 296, 530, 557
BtsCI GGATG 2 cut(s) 54, 171
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 1 cut(s) 125
Cac8I GCNNGC 2 cut(s) 328, 569
CaiI CAGNNNCTG 1 cut(s) 291
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 294, 555
Csp6I GTAC 1 cut(s) 94
CspAI ACCGGT 1 cut(s) 187
CviAII CATG 5 cut(s) 40, 109, 436, 494, 552
CviQI GTAC 1 cut(s) 94
DpnI GATC 2 cut(s) 149, 264
DpnII GATC 2 cut(s) 147, 262
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 351, 635
EarI CTCTTC 2 cut(s) 351, 635
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 2 cut(s) 125, 280
Eco57I CTGAAG 2 cut(s) 416, 502
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 2 cut(s) 279, 456
EcoT38I GRGCYC 2 cut(s) 125, 280
FaeI CATG 5 cut(s) 43, 112, 439, 497, 555
FalI AAGNNNNNCTT 4 cut(s) 399, 431, 551, 583
FatI CATG 5 cut(s) 39, 108, 435, 493, 551
FauI CCCGC 1 cut(s) 319
Fnu4HI GCNGC 1 cut(s) 369
FokI GGATG 2 cut(s) 61, 158
FriOI GRGCYC 2 cut(s) 125, 280
Fsp4HI GCNGC 1 cut(s) 369
GluI GCNGC 1 cut(s) 369
HaeIII GGCC 5 cut(s) 71, 284, 296, 530, 557
HapII CCGG 2 cut(s) 164, 188
Hin1II CATG 5 cut(s) 43, 112, 439, 497, 555
HinfI GANTC 4 cut(s) 81, 160, 197, 288
HpaII CCGG 2 cut(s) 164, 188
HphI GGTGA 1 cut(s) 452
Hpy188I TCNGA 2 cut(s) 59, 262
Hpy188III TCNNGA 2 cut(s) 394, 629
HpyAV CCTTC 5 cut(s) 70, 229, 391, 401, 568
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4V TGCA 2 cut(s) 214, 571
Hsp92II CATG 5 cut(s) 43, 112, 439, 497, 555
Kzo9I GATC 2 cut(s) 147, 262
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 1 cut(s) 380
LweI GCATC 1 cut(s) 313
MalI GATC 2 cut(s) 149, 264
MboI GATC 2 cut(s) 147, 262
MboII GAAGA 8 cut(s) 113, 116, 169, 346, 368, 617, 649, 652
MhlI GDGCHC 4 cut(s) 125, 171, 280, 539
MluCI AATT 1 cut(s) 380
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 9 cut(s) 4, 45, 105, 258, 268, 358, 520, 555, 636
MseI TTAA 1 cut(s) 135
MslI CAYNNNNRTG 2 cut(s) 209, 498
MspA1I CMGCKG 1 cut(s) 486
MspCI CTTAAG 1 cut(s) 134
MspI CCGG 2 cut(s) 164, 188
MspR9I CCNGG 2 cut(s) 281, 458
MvaI CCWGG 2 cut(s) 281, 458
NdeII GATC 2 cut(s) 147, 262
NlaIII CATG 5 cut(s) 43, 112, 439, 497, 555
NlaIV GGNNCC 2 cut(s) 246, 455
NmeAIII GCCGAG 2 cut(s) 97, 556
NspI RCATGY 1 cut(s) 43
PciSI GCTCTTC 1 cut(s) 351
PfeI GAWTC 3 cut(s) 81, 160, 288
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 187
PkrI GCNGC 1 cut(s) 370
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 279, 456
PspGI CCWGG 2 cut(s) 279, 456
PspN4I GGNNCC 2 cut(s) 246, 455
PspPI GGNCC 2 cut(s) 294, 555
PstNI CAGNNNCTG 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 486
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 2 cut(s) 209, 498
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 1 cut(s) 369
Sau3AI GATC 2 cut(s) 147, 262
Sau96I GGNCC 2 cut(s) 294, 555
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 2 cut(s) 281, 458
SduI GDGCHC 4 cut(s) 125, 171, 280, 539
SetI ASST 8 cut(s) 15, 67, 402, 417, 488, 546, 566, 615
SfaNI GCATC 1 cut(s) 313
SfcI CTRYAG 1 cut(s) 481
SmiMI CAYNNNNRTG 2 cut(s) 209, 498
SmlI CTYRAG 2 cut(s) 134, 629
SmoI CTYRAG 2 cut(s) 134, 629
Sse9I AATT 1 cut(s) 380
SsiI CCGC 2 cut(s) 5, 326
StyD4I CCNGG 2 cut(s) 279, 456
TaaI ACNGT 1 cut(s) 29
TasI AATT 1 cut(s) 380
TatI WGTACW 1 cut(s) 93
TfiI GAWTC 3 cut(s) 81, 160, 288
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 1 cut(s) 132
TseI GCWGC 1 cut(s) 368
TspDTI ATGAA 8 cut(s) 155, 240, 292, 373, 424, 482, 540, 650
TspRI CASTG 1 cut(s) 132
Van91I CCANNNNNTGG 1 cut(s) 291
Vha464I CTTAAG 1 cut(s) 134
XapI RAATTY 1 cut(s) 380
XceI RCATGY 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.