MD10G1197100.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000304
Physical Location & Seq
Reverse (-)
29507624 .. 29509326
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1197100.v1.1.491

Sequence Viewer

Length: 660 bp
ATGGCGGATGAGGTTGTTCTTCTGGACTTCTGGGCAAGCATGTTTGGCATGAGGGCCAGAGTAGCGCTGGCGGAGAAGGGCATCGAGTATGAGTACAGAGAGGAGGACTTGAGGAACAAGAGCCAGCTGCTTCTGCAGATGAACCCGGTTCACAAGAAGATCCCGGTTCTCATTCACAACGGTAAGCCAGTCTGTGAGTCACTCATCATTGTGCAGTATATTGATGAAGTTTGGAGTGATAAAGCTCCTTTGCTTCCCTCTGAGCCTTACCAGAGAGCTTGCTCTAGGTTCTGGGCTGATTTCATTGACAAGAAGCTATATGATGCTGGGAGGAAGATATGGTCCACAAAGGGAGAGGAACAAGAGGCAGCTAAAAAGGACTTCATCGAAATCCTGAAGCAGCTGGAGGGACAGCTGGGAGGCAAGCCATATTTTGAGGGTGAGAAATTTGGGTTCTTGGACATTGCTCTCATCACGTTCTACAGCTGGTTCCATGCATATGAGACCTGTGGAAACTTCAGCATCGAGGCCGAGTGTCCCAAGCTCATTGCATGGGCCAAGCGGTGCAAGCAGAGGGAGAGTGTTTCCAAATCCCTTGCTGACGAAAAGAAGGTGTATGAGTTCGTTCTTAGCATTAAGAAGATGCTTGGTGTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016740 GO:0016765 GO:0018973 GO:0018974 GO:0019326 GO:0019439 GO:0019748 GO:0019899 GO:0031090 GO:0031668 GO:0033218 GO:0033554 GO:0034641 GO:0042221 GO:0042277 GO:0042537 GO:0042631 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046256 GO:0046260 GO:0046263 GO:0046686 GO:0048037 GO:0048046 GO:0048583 GO:0050789 GO:0050896 GO:0051186 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071944 GO:0072341 GO:0072490 GO:0072491 GO:0080167 GO:0098588 GO:0098754 GO:0098805 GO:0104004 GO:1900750 GO:1901360 GO:1901361 GO:1901564 GO:1901575 GO:1901681 GO:1901700 GO:1901701 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

220

Amino Acids

25.39

Weight (kDa)

6.05

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 2.4e-21 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 76 8.5e-15 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 3.2e-13 Glutathione S-transferase, N-terminal domain
GST_C PF00043 116 - 193 2.4e-08 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 121 - 188 1e-09 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 71, 562
AclWI GGATC 1 cut(s) 154
AcsI RAATTY 1 cut(s) 446
AcuI CTGAAG 2 cut(s) 416, 502
AfaI GTAC 1 cut(s) 95
AfeI AGCGCT 1 cut(s) 66
AluBI AGCT 9 cut(s) 127, 245, 278, 316, 371, 403, 415, 486, 544
AluI AGCT 9 cut(s) 127, 245, 278, 316, 371, 403, 415, 486, 544
Alw26I GTCTC 1 cut(s) 497
AlwI GGATC 1 cut(s) 154
Aor51HI AGCGCT 1 cut(s) 66
AoxI GGCC 3 cut(s) 54, 528, 555
ApeKI GCWGC 3 cut(s) 127, 368, 400
ApoI RAATTY 1 cut(s) 446
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 3 cut(s) 54, 342, 555
AsuC2I CCSGG 2 cut(s) 146, 164
AsuHPI GGTGA 1 cut(s) 452
AvaII GGWCC 1 cut(s) 342
BbvI GCAGC 3 cut(s) 114, 380, 412
BcnI CCSGG 2 cut(s) 146, 164
BcoDI GTCTC 1 cut(s) 497
BfaI CTAG 1 cut(s) 285
BfmI CTRYAG 2 cut(s) 134, 481
BfoI RGCGCY 1 cut(s) 68
BisI GCNGC 3 cut(s) 128, 369, 401
BlsI GCNGC 3 cut(s) 129, 370, 402
Bme1390I CCNGG 2 cut(s) 146, 164
Bme18I GGWCC 1 cut(s) 342
BmgT120I GGNCC 3 cut(s) 54, 342, 555
BmiI GGNNCC 1 cut(s) 491
BmrFI CCNGG 2 cut(s) 146, 164
BmsI GCATC 4 cut(s) 90, 313, 531, 633
BpmI CTGGAG 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 130
BpuMI CCSGG 2 cut(s) 146, 164
BsaI GGTCTC 1 cut(s) 497
Bse1I ACTGG 1 cut(s) 188
Bse3DI GCAATG 2 cut(s) 462, 546
BseGI GGATG 1 cut(s) 13
BseMI GCAATG 2 cut(s) 462, 546
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 116
BseXI GCAGC 3 cut(s) 114, 380, 412
BseYI CCCAGC 2 cut(s) 326, 415
BsgI GTGCAG 1 cut(s) 233
BshFI GGCC 3 cut(s) 56, 530, 557
BsiSI CCGG 2 cut(s) 146, 164
BslFI GGGAC 2 cut(s) 423, 522
BsmAI GTCTC 1 cut(s) 497
BsmFI GGGAC 2 cut(s) 423, 522
BsnI GGCC 3 cut(s) 56, 530, 557
Bso31I GGTCTC 1 cut(s) 497
Bsp143I GATC 1 cut(s) 159
BspACI CCGC 3 cut(s) 5, 71, 562
BspANI GGCC 3 cut(s) 56, 530, 557
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 491
BspMAI CTGCAG 1 cut(s) 138
BspPI GGATC 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 497
BsrDI GCAATG 2 cut(s) 462, 546
BsrI ACTGG 1 cut(s) 188
BssMI GATC 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 182
BstC8I GCNNGC 6 cut(s) 37, 69, 125, 280, 425, 569
BstDEI CTNAG 2 cut(s) 261, 629
BstF5I GGATG 1 cut(s) 13
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 1 cut(s) 162
BstMAI GTCTC 1 cut(s) 497
BstMBI GATC 1 cut(s) 159
BstMWI GCNNNNNNNGC 4 cut(s) 45, 62, 133, 568
BstNSI RCATGY 1 cut(s) 43
BstSCI CCNGG 2 cut(s) 144, 162
BstSFI CTRYAG 2 cut(s) 134, 481
BstV1I GCAGC 3 cut(s) 114, 380, 412
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 3 cut(s) 56, 530, 557
BtsCI GGATG 1 cut(s) 13
Cac8I GCNNGC 6 cut(s) 37, 69, 125, 280, 425, 569
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 3 cut(s) 54, 342, 555
Csp6I GTAC 1 cut(s) 94
CviAII CATG 4 cut(s) 40, 49, 494, 552
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 2 cut(s) 261, 629
DpnI GATC 1 cut(s) 161
DpnII GATC 1 cut(s) 159
EciI GGCGGA 2 cut(s) 20, 86
Eco31I GGTCTC 1 cut(s) 497
Eco47I GGWCC 1 cut(s) 342
Eco47III AGCGCT 1 cut(s) 66
Eco57I CTGAAG 2 cut(s) 416, 502
EcoT22I ATGCAT 1 cut(s) 499
FaeI CATG 4 cut(s) 43, 52, 497, 555
FaqI GGGAC 2 cut(s) 423, 522
FatI CATG 4 cut(s) 39, 48, 493, 551
FauNDI CATATG 1 cut(s) 499
Fnu4HI GCNGC 3 cut(s) 128, 369, 401
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 128, 369, 401
FspBI CTAG 1 cut(s) 285
GlaI GCGC 1 cut(s) 66
GluI GCNGC 3 cut(s) 128, 369, 401
GsaI CCCAGC 2 cut(s) 330, 419
GsuI CTGGAG 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 3 cut(s) 56, 530, 557
HapII CCGG 2 cut(s) 146, 164
HhaI GCGC 1 cut(s) 67
Hin1II CATG 4 cut(s) 43, 52, 497, 555
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HinfI GANTC 1 cut(s) 197
HpaII CCGG 2 cut(s) 146, 164
HphI GGTGA 1 cut(s) 452
Hpy166II GTNNAC 2 cut(s) 151, 345
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 2 cut(s) 23, 394
Hpy8I GTNNAC 2 cut(s) 151, 345
HpyAV CCTTC 2 cut(s) 70, 604
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4IV ACGT 1 cut(s) 476
HpyCH4V TGCA 5 cut(s) 136, 214, 497, 551, 567
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 62, 133, 568
HpyF3I CTNAG 2 cut(s) 261, 629
HpySE526I ACGT 1 cut(s) 476
Hsp92II CATG 4 cut(s) 43, 52, 497, 555
HspAI GCGC 1 cut(s) 65
Kzo9I GATC 1 cut(s) 159
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 3 cut(s) 114, 380, 412
LweI GCATC 4 cut(s) 90, 313, 531, 633
MaeI CTAG 1 cut(s) 285
MaeII ACGT 1 cut(s) 476
MaeIII GTNAC 1 cut(s) 198
MalI GATC 1 cut(s) 161
MboI GATC 1 cut(s) 159
MboII GAAGA 4 cut(s) 11, 169, 346, 652
MflI RGATCY 1 cut(s) 159
MluCI AATT 1 cut(s) 446
MlyI GAGTC 1 cut(s) 206
Mph1103I ATGCAT 1 cut(s) 499
MseI TTAA 1 cut(s) 636
MslI CAYNNNNRTG 2 cut(s) 209, 498
MspA1I CMGCKG 4 cut(s) 127, 403, 415, 486
MspI CCGG 2 cut(s) 146, 164
MspR9I CCNGG 2 cut(s) 146, 164
MwoI GCNNNNNNNGC 4 cut(s) 45, 62, 133, 568
NciI CCSGG 2 cut(s) 146, 164
NdeI CATATG 1 cut(s) 499
NdeII GATC 1 cut(s) 159
NlaIII CATG 4 cut(s) 43, 52, 497, 555
NlaIV GGNNCC 1 cut(s) 491
NmeAIII GCCGAG 1 cut(s) 556
NmuCI GTSAC 1 cut(s) 198
NsiI ATGCAT 1 cut(s) 499
NspI RCATGY 1 cut(s) 43
PkrI GCNGC 3 cut(s) 129, 370, 402
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PspFI CCCAGC 2 cut(s) 326, 415
PspN4I GGNNCC 1 cut(s) 491
PspPI GGNCC 3 cut(s) 54, 342, 555
PstI CTGCAG 1 cut(s) 138
PsuI RGATCY 1 cut(s) 159
PvuII CAGCTG 4 cut(s) 127, 403, 415, 486
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 2 cut(s) 209, 498
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 3 cut(s) 128, 369, 401
Sau3AI GATC 1 cut(s) 159
Sau96I GGNCC 3 cut(s) 54, 342, 555
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 2 cut(s) 146, 164
SfaNI GCATC 4 cut(s) 90, 313, 531, 633
SfcI CTRYAG 2 cut(s) 134, 481
SinI GGWCC 1 cut(s) 342
SmiMI CAYNNNNRTG 2 cut(s) 209, 498
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
Sse9I AATT 1 cut(s) 446
SsiI CCGC 3 cut(s) 5, 71, 562
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 144, 162
TaaI ACNGT 1 cut(s) 182
TaiI ACGT 1 cut(s) 479
TaqI TCGA 3 cut(s) 84, 387, 525
TasI AATT 1 cut(s) 446
TatI WGTACW 1 cut(s) 93
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TseFI GTSAC 1 cut(s) 198
TseI GCWGC 3 cut(s) 127, 368, 400
Tsp45I GTSAC 1 cut(s) 198
TspDTI ATGAA 4 cut(s) 155, 240, 292, 373
VpaK11BI GGWCC 1 cut(s) 342
XapI RAATTY 1 cut(s) 446
XceI RCATGY 1 cut(s) 43
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XspI CTAG 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.