RchiOBHm_Chr5g0027361

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000304
Physical Location & Seq
Reverse (-)
21361645 .. 21362790
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30691

Sequence Viewer

Length: 765 bp
ATGGCGGATGAGGTTGTGTTGTTGGATTTCTGGCCCAGCCCTTTTGGGGTGAGGCTGAGGATCGCTCTGGCCGAGAAAGGCATCAAGTACGAGTACAAGGAAGAGGACCTGGAGAACAAGAGCCCACTCTTGCTGCAGTCGAATCCGGTTTACAAGCAGATCCCGGTTCTCATTCACAACGGCAAAGCGGTCTGCGAGTCCCTTATTGCGCTTCAGTACATTGACGAGGTTTGGAACGATAAGGCTCCACTCTTGCCCTTTGATCCTTACCTTAGATCCCAAGCAAAGTTCTGGGCCGACTTCGTGGACAAGAAGATATATGATTTGGGGAGGAAGGCATGCACAACTAAAGGAAAAGAACACGAAGCGGCACAGAAGGAATTCATGAACTGCATTAAGTCGCTAGAAGTGGAGCTTGGAGACAAGCCCTTCTTTGGGGGCGAGACCCTCGGGGTCGTGGACGTGTCACTCGTTCCTTTCTTTTCCTGGTTCTCTGTGTATGAGAAATTTGGTAACTTCAGTATAGAGCTGGAGTGCCCAAAGTTCAATGCATGGATTAAAAGGTGTTTGGAGAAGGAGAGTGTGTCCAAGTCCCTTCCAGACCAGGATAAGATCTTTGAAATTTTTGTGGATATCAGGAAAAAGCTTGGAATGGATCGAATAATGACATTTTGTGGTCAAGTTGTTGTGTGTAGAGCAAAGAGTTCGAATATTCTCGATCAGTTTCTTACCCTAAATGAATTTTGTTCTTGTTACAGTGAATAA

Protein Analysis

254

Amino Acids

29.25

Weight (kDa)

5.35

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 1.2e-19 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 12 - 76 7.3e-12 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 1.1e-12 Glutathione S-transferase, N-terminal domain
GST_C PF00043 115 - 192 3.8e-06 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 121 - 188 5.2e-11 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 188, 368
AclWI GGATC 5 cut(s) 68, 154, 257, 270, 663
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 4 cut(s) 380, 506, 621, 740
AcuI CTGAAG 2 cut(s) 197, 502
AfaI GTAC 3 cut(s) 89, 95, 218
AfiI CCNNNNNNNGG 3 cut(s) 46, 434, 435
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 2 cut(s) 547, 620
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 3 cut(s) 108, 485, 603
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 3 cut(s) 415, 529, 646
AluI AGCT 3 cut(s) 415, 529, 646
Alw26I GTCTC 2 cut(s) 414, 437
AlwI GGATC 5 cut(s) 68, 154, 257, 270, 663
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 3 cut(s) 32, 69, 294
ApeKI GCWGC 1 cut(s) 133
ApoI RAATTY 4 cut(s) 380, 506, 621, 740
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 3 cut(s) 33, 106, 294
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 61
AsuII TTCGAA 1 cut(s) 707
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 539
BanII GRGCYC 1 cut(s) 125
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 120
BceAI ACGGC 1 cut(s) 196
BcgI CGANNNNNNTGC 2 cut(s) 687, 721
BciT130I CCWGG 3 cut(s) 110, 487, 605
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 2 cut(s) 414, 437
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 1 cut(s) 134
BglII AGATCT 1 cut(s) 612
BisI GCNGC 2 cut(s) 134, 369
BlsI GCNGC 2 cut(s) 135, 370
Bme1390I CCNGG 4 cut(s) 110, 164, 487, 605
Bme18I GGWCC 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 449
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 3 cut(s) 33, 106, 294
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 4 cut(s) 110, 164, 487, 605
BmsI GCATC 1 cut(s) 90
BplI GAGNNNNNCTC 2 cut(s) 49, 81
BpmI CTGGAG 2 cut(s) 131, 551
Bpu10I CCTNAGC 1 cut(s) 56
Bpu14I TTCGAA 1 cut(s) 707
BpuMI CCSGG 1 cut(s) 164
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 448
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 2 cut(s) 569, 599
Bsc4I CCNNNNNNNGG 3 cut(s) 46, 434, 435
BseBI CCWGG 3 cut(s) 110, 487, 605
BseDI CCNNGG 1 cut(s) 448
BseGI GGATG 1 cut(s) 13
BseLI CCNNNNNNNGG 3 cut(s) 46, 434, 435
BseMII CTCAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 539
BseXI GCAGC 1 cut(s) 120
BseYI CCCAGC 1 cut(s) 35
BshFI GGCC 3 cut(s) 34, 71, 296
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 2 cut(s) 146, 164
BslFI GGGAC 2 cut(s) 184, 577
BslI CCNNNNNNNGG 3 cut(s) 46, 434, 435
BsmAI GTCTC 2 cut(s) 414, 437
BsmFI GGGAC 2 cut(s) 184, 577
BsnI GGCC 3 cut(s) 34, 71, 296
Bso31I GGTCTC 1 cut(s) 437
BsoBI CYCGRG 1 cut(s) 449
Bsp119I TTCGAA 1 cut(s) 707
Bsp1286I GDGCHC 2 cut(s) 125, 539
Bsp143I GATC 7 cut(s) 60, 159, 262, 275, 612, 655, 718
BspACI CCGC 3 cut(s) 5, 188, 368
BspANI GGCC 3 cut(s) 34, 71, 296
BspCNI CTCAG 1 cut(s) 48
BspHI TCATGA 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 246
BspMAI CTGCAG 1 cut(s) 138
BspPI GGATC 5 cut(s) 68, 154, 257, 270, 663
BspT104I TTCGAA 1 cut(s) 707
BspTNI GGTCTC 1 cut(s) 437
BssECI CCNNGG 1 cut(s) 448
BssMI GATC 7 cut(s) 60, 159, 262, 275, 612, 655, 718
Bst2UI CCWGG 3 cut(s) 110, 487, 605
Bst4CI ACNGT 1 cut(s) 758
Bst6I CTCTTC 1 cut(s) 96
BstBI TTCGAA 1 cut(s) 707
BstC8I GCNNGC 1 cut(s) 340
BstDEI CTNAG 2 cut(s) 56, 272
BstF5I GGATG 1 cut(s) 13
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 7 cut(s) 63, 162, 265, 278, 615, 658, 721
BstMAI GTCTC 2 cut(s) 414, 437
BstMBI GATC 7 cut(s) 60, 159, 262, 275, 612, 655, 718
BstNI CCWGG 3 cut(s) 110, 487, 605
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 4 cut(s) 108, 162, 485, 603
BstSFI CTRYAG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 539
BstV1I GCAGC 1 cut(s) 120
BstX2I RGATCY 3 cut(s) 159, 275, 612
BstYI RGATCY 3 cut(s) 159, 275, 612
BsuRI GGCC 3 cut(s) 34, 71, 296
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 1 cut(s) 13
BtsIMutI CAGTG 1 cut(s) 763
Cac8I GCNNGC 1 cut(s) 340
CciI TCATGA 1 cut(s) 384
CfoI GCGC 1 cut(s) 211
Cfr13I GGNCC 3 cut(s) 33, 106, 294
Csp6I GTAC 3 cut(s) 88, 94, 217
CviAII CATG 3 cut(s) 339, 385, 552
CviQI GTAC 3 cut(s) 88, 94, 217
DdeI CTNAG 2 cut(s) 56, 272
DpnI GATC 7 cut(s) 62, 161, 264, 277, 614, 657, 720
DpnII GATC 7 cut(s) 60, 159, 262, 275, 612, 655, 718
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 437
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 2 cut(s) 197, 502
Eco88I CYCGRG 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 106
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 3 cut(s) 108, 485, 603
EcoRV GATATC 1 cut(s) 634
EcoT22I ATGCAT 1 cut(s) 553
EcoT38I GRGCYC 1 cut(s) 125
FaeI CATG 3 cut(s) 342, 388, 555
FaiI YATR 7 cut(s) 319, 321, 340, 386, 501, 524, 553
FalI AAGNNNNNCTT 4 cut(s) 399, 431, 416, 448
FaqI GGGAC 2 cut(s) 184, 577
FatI CATG 3 cut(s) 338, 384, 551
Fnu4HI GCNGC 2 cut(s) 134, 369
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 2 cut(s) 134, 369
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 210
GluI GCNGC 2 cut(s) 134, 369
GsaI CCCAGC 1 cut(s) 39
GsuI CTGGAG 2 cut(s) 131, 551
HaeIII GGCC 3 cut(s) 34, 71, 296
HapII CCGG 2 cut(s) 146, 164
HhaI GCGC 1 cut(s) 211
Hin1II CATG 3 cut(s) 342, 388, 555
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 2 cut(s) 142, 197
HpaII CCGG 2 cut(s) 146, 164
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 3 cut(s) 151, 307, 460
Hpy188III TCNNGA 4 cut(s) 385, 599, 637, 716
Hpy8I GTNNAC 3 cut(s) 151, 307, 460
HpyAV CCTTC 5 cut(s) 328, 370, 439, 568, 605
HpyCH4III ACNGT 1 cut(s) 758
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 4 cut(s) 136, 342, 393, 551
HpyF3I CTNAG 2 cut(s) 56, 272
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 3 cut(s) 342, 388, 555
HspAI GCGC 1 cut(s) 209
Kzo9I GATC 7 cut(s) 60, 159, 262, 275, 612, 655, 718
LmnI GCTCC 2 cut(s) 250, 412
Lsp1109I GCAGC 1 cut(s) 120
LweI GCATC 1 cut(s) 90
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 3 cut(s) 465, 512, 752
MalI GATC 7 cut(s) 62, 161, 264, 277, 614, 657, 720
MboI GATC 7 cut(s) 60, 159, 262, 275, 612, 655, 718
MboII GAAGA 2 cut(s) 113, 325
MflI RGATCY 3 cut(s) 159, 275, 612
MhlI GDGCHC 2 cut(s) 125, 539
MluCI AATT 4 cut(s) 380, 506, 621, 740
MlyI GAGTC 1 cut(s) 206
MnlI CCTC 7 cut(s) 4, 45, 51, 97, 220, 324, 458
Mph1103I ATGCAT 1 cut(s) 553
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 2 cut(s) 396, 558
MspI CCGG 2 cut(s) 146, 164
MspR9I CCNGG 4 cut(s) 110, 164, 487, 605
MvaI CCWGG 3 cut(s) 110, 487, 605
NciI CCSGG 1 cut(s) 164
NdeII GATC 7 cut(s) 60, 159, 262, 275, 612, 655, 718
NlaIII CATG 3 cut(s) 342, 388, 555
NlaIV GGNNCC 1 cut(s) 246
NmeAIII GCCGAG 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 465
NsiI ATGCAT 1 cut(s) 553
NspI RCATGY 1 cut(s) 342
NspV TTCGAA 1 cut(s) 707
PaeI GCATGC 1 cut(s) 342
PagI TCATGA 1 cut(s) 384
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 468
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 142
PkrI GCNGC 2 cut(s) 135, 370
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PpuMI RGGWCCY 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 106
Psp6I CCWGG 3 cut(s) 108, 485, 603
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 3 cut(s) 108, 485, 603
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 3 cut(s) 33, 106, 294
PspPPI RGGWCCY 1 cut(s) 106
PstI CTGCAG 1 cut(s) 138
PsuI RGATCY 3 cut(s) 159, 275, 612
RsaI GTAC 3 cut(s) 89, 95, 218
RsaNI GTAC 3 cut(s) 88, 94, 217
SaqAI TTAA 2 cut(s) 396, 558
SatI GCNGC 2 cut(s) 134, 369
Sau3AI GATC 7 cut(s) 60, 159, 262, 275, 612, 655, 718
Sau96I GGNCC 3 cut(s) 33, 106, 294
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 4 cut(s) 110, 164, 487, 605
SduI GDGCHC 2 cut(s) 125, 539
SetI ASST 9 cut(s) 15, 111, 231, 273, 417, 465, 531, 566, 648
SfaNI GCATC 1 cut(s) 90
SfcI CTRYAG 1 cut(s) 134
SfuI TTCGAA 1 cut(s) 707
SinI GGWCC 1 cut(s) 106
SphI GCATGC 1 cut(s) 342
Sse9I AATT 4 cut(s) 380, 506, 621, 740
SsiI CCGC 3 cut(s) 5, 188, 368
SspI AATATT 1 cut(s) 712
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 4 cut(s) 108, 162, 485, 603
TaaI ACNGT 1 cut(s) 758
TaiI ACGT 1 cut(s) 465
TaqI TCGA 4 cut(s) 140, 658, 707, 717
TasI AATT 4 cut(s) 380, 506, 621, 740
TatI WGTACW 2 cut(s) 93, 216
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 2 cut(s) 396, 558
Tru9I TTAA 2 cut(s) 396, 558
TscAI CASTG 1 cut(s) 763
TseFI GTSAC 1 cut(s) 465
TseI GCWGC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 465
TspDTI ATGAA 3 cut(s) 373, 401, 753
TspRI CASTG 1 cut(s) 763
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 4 cut(s) 380, 506, 621, 740
XceI RCATGY 1 cut(s) 342
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 1 cut(s) 404
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.