FvH4_3g16250

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000304
Physical Location & Seq
Reverse (-)
10243839 .. 10245768
1930 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16250.t1

Sequence Viewer

Length: 657 bp
ATGGCGGATGAGGTTGTCTTGTTGGACTTCTGGCCTAGCCCATTTGGGATGAGGCTGAGGATCGCTCTGGCCGAGAAAGGCGTCAAGTACGAGTACAAGGACGAGGACCTGAGGAACAAGAGCCCGCTGTTGCTTCAGTCGAACCCGGTTCACAAGAAGATCCCGGTTCTCATTCACAACGGCAAACCTGTCTGCGAGTCTGTCATTGCTCTTCAGTACATTGACGAGGTCTGGACTAACAAGCCACTATTGCCCTCCGACCCTTACCTCAGATCCCAGGCCAGGTTCTGGGCCGACTTTGTGGACAAGAAGATATATGATATCGGTAGGAAGACATGGACAACGAAAGGAGATGAGCAGGAGGCAGCAAAGAAGGAATTCATCGACTGCATTAAGTTGCTAGAAGTGGAGCTTGGGGACAAGCCTTTCTTTGGCGGTGAGACCCTCGGATTTGTGGACGTGACGCTCGTTCCTTTCTATTCCTGGTTCTCTGTGTATGAGAAATACGGCAACTTCAGCATTGCGCCAGAGTGCCCAAAGTTCATGGCTTGGGTTAAGAGGTGTATGGAGAAGGAGAGTGTGTCAAAGTCTCTTCCTGACCAGGACAAGGTCTGTGGCTTTGTTGCCGAGATGAGGAAGAAGCTTGGAGTTGAGTAG

Protein Analysis

219

Amino Acids

25.14

Weight (kDa)

6.22

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 1.2e-20 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 12 - 76 8.2e-12 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 5e-12 Glutathione S-transferase, N-terminal domain
GST_C PF00043 115 - 191 6.3e-07 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 120 - 187 3e-10 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 288
AciI CCGC 3 cut(s) 5, 125, 435
AclWI GGATC 3 cut(s) 68, 154, 267
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 3 cut(s) 119, 197, 499
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 3 cut(s) 89, 95, 218
AfiI CCNNNNNNNGG 5 cut(s) 282, 288, 431, 607, 633
AjiI CACGTC 1 cut(s) 460
AjnI CCWGG 4 cut(s) 276, 281, 482, 600
AjuI GAANNNNNNNTTGG 2 cut(s) 396, 428
AluBI AGCT 2 cut(s) 412, 643
AluI AGCT 2 cut(s) 412, 643
Alw26I GTCTC 2 cut(s) 434, 594
AlwI GGATC 3 cut(s) 68, 154, 267
AlwNI CAGNNNCTG 1 cut(s) 288
AoxI GGCC 4 cut(s) 32, 69, 279, 291
ApeKI GCWGC 1 cut(s) 365
ApoI RAATTY 1 cut(s) 377
Asp700I GAANNNNTTC 1 cut(s) 377
AspLEI GCGC 1 cut(s) 526
AspS9I GGNCC 2 cut(s) 106, 291
AsuC2I CCSGG 2 cut(s) 146, 164
AsuHPI GGTGA 1 cut(s) 449
AvaII GGWCC 1 cut(s) 106
AxyI CCTNAGG 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 536
BanII GRGCYC 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 338
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 377
BceAI ACGGC 2 cut(s) 196, 523
BciT130I CCWGG 4 cut(s) 278, 283, 484, 602
BcnI CCSGG 2 cut(s) 146, 164
BcoDI GTCTC 2 cut(s) 434, 594
BfaI CTAG 2 cut(s) 36, 401
BisI GCNGC 1 cut(s) 366
BlsI GCNGC 1 cut(s) 367
Bme1390I CCNGG 6 cut(s) 146, 164, 278, 283, 484, 602
Bme18I GGWCC 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 460
BmgT120I GGNCC 2 cut(s) 106, 291
BmrFI CCNGG 6 cut(s) 146, 164, 278, 283, 484, 602
BpiI GAAGAC 1 cut(s) 338
BplI GAGNNNNNCTC 2 cut(s) 49, 81
Bpu10I CCTNAGC 1 cut(s) 56
BpuMI CCSGG 2 cut(s) 146, 164
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 2 cut(s) 276, 445
BsaXI ACNNNNNCTCC 2 cut(s) 566, 596
Bsc4I CCNNNNNNNGG 5 cut(s) 282, 288, 431, 607, 633
Bse21I CCTNAGG 1 cut(s) 110
Bse3DI GCAATG 2 cut(s) 204, 519
BseBI CCWGG 4 cut(s) 278, 283, 484, 602
BseDI CCNNGG 2 cut(s) 276, 445
BseGI GGATG 2 cut(s) 13, 54
BseLI CCNNNNNNNGG 5 cut(s) 282, 288, 431, 607, 633
BseMI GCAATG 2 cut(s) 204, 519
BseMII CTCAG 3 cut(s) 47, 101, 283
BseSI GKGCMC 1 cut(s) 536
BseXI GCAGC 1 cut(s) 377
BshFI GGCC 4 cut(s) 34, 71, 281, 293
BsiSI CCGG 2 cut(s) 146, 164
BslFI GGGAC 1 cut(s) 431
BslI CCNNNNNNNGG 5 cut(s) 282, 288, 431, 607, 633
BsmAI GTCTC 2 cut(s) 434, 594
BsmFI GGGAC 1 cut(s) 431
BsnI GGCC 4 cut(s) 34, 71, 281, 293
Bso31I GGTCTC 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 125, 536
Bsp143I GATC 3 cut(s) 60, 159, 272
BspACI CCGC 3 cut(s) 5, 125, 435
BspANI GGCC 4 cut(s) 34, 71, 281, 293
BspCNI CTCAG 3 cut(s) 48, 102, 282
BspPI GGATC 3 cut(s) 68, 154, 267
BspQI GCTCTTC 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 434
BsrDI GCAATG 2 cut(s) 204, 519
BssECI CCNNGG 2 cut(s) 276, 445
BssMI GATC 3 cut(s) 60, 159, 272
BssNI GRCGYC 1 cut(s) 81
Bst2UI CCWGG 4 cut(s) 278, 283, 484, 602
Bst6I CTCTTC 2 cut(s) 216, 597
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 3 cut(s) 56, 110, 269
BstF5I GGATG 2 cut(s) 13, 54
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 3 cut(s) 63, 162, 275
BstMAI GTCTC 2 cut(s) 434, 594
BstMBI GATC 3 cut(s) 60, 159, 272
BstMWI GCNNNNNNNGC 2 cut(s) 250, 516
BstNI CCWGG 4 cut(s) 278, 283, 484, 602
BstSCI CCNGG 6 cut(s) 144, 162, 276, 281, 482, 600
BstSLI GKGCMC 1 cut(s) 536
BstV1I GCAGC 1 cut(s) 377
BstV2I GAAGAC 1 cut(s) 338
BstX2I RGATCY 2 cut(s) 159, 272
BstYI RGATCY 2 cut(s) 159, 272
Bsu36I CCTNAGG 1 cut(s) 110
BsuRI GGCC 4 cut(s) 34, 71, 281, 293
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 2 cut(s) 13, 54
Cac8I GCNNGC 1 cut(s) 125
CaiI CAGNNNCTG 1 cut(s) 288
CfoI GCGC 1 cut(s) 526
Cfr13I GGNCC 2 cut(s) 106, 291
CseI GACGC 2 cut(s) 70, 472
Csp6I GTAC 3 cut(s) 88, 94, 217
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CviAII CATG 2 cut(s) 336, 544
CviQI GTAC 3 cut(s) 88, 94, 217
DdeI CTNAG 3 cut(s) 56, 110, 269
DpnI GATC 3 cut(s) 62, 161, 274
DpnII GATC 3 cut(s) 60, 159, 272
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 216, 597
EarI CTCTTC 2 cut(s) 216, 597
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 434
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 3 cut(s) 119, 197, 499
Eco81I CCTNAGG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 106
EcoRI GAATTC 1 cut(s) 377
EcoRII CCWGG 4 cut(s) 276, 281, 482, 600
EcoRV GATATC 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 125
FaeI CATG 2 cut(s) 339, 547
FaiI YATR 6 cut(s) 316, 318, 337, 498, 545, 566
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 413, 445
FaqI GGGAC 1 cut(s) 431
FatI CATG 2 cut(s) 335, 543
FauI CCCGC 1 cut(s) 132
Fnu4HI GCNGC 1 cut(s) 366
FokI GGATG 2 cut(s) 20, 61
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 1 cut(s) 366
FspBI CTAG 2 cut(s) 36, 401
GlaI GCGC 1 cut(s) 525
GluI GCNGC 1 cut(s) 366
HaeIII GGCC 4 cut(s) 34, 71, 281, 293
HapII CCGG 2 cut(s) 146, 164
HgaI GACGC 2 cut(s) 70, 472
HhaI GCGC 1 cut(s) 526
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 2 cut(s) 339, 547
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 1 cut(s) 197
HpaII CCGG 2 cut(s) 146, 164
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 3 cut(s) 151, 304, 457
Hpy188I TCNGA 3 cut(s) 259, 272, 449
Hpy188III TCNNGA 2 cut(s) 232, 596
Hpy8I GTNNAC 3 cut(s) 151, 304, 457
HpyAV CCTTC 2 cut(s) 367, 565
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 1 cut(s) 390
HpyF10VI GCNNNNNNNGC 2 cut(s) 250, 516
HpyF3I CTNAG 3 cut(s) 56, 110, 269
HpySE526I ACGT 1 cut(s) 459
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 2 cut(s) 339, 547
HspAI GCGC 1 cut(s) 524
Kzo9I GATC 3 cut(s) 60, 159, 272
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 1 cut(s) 377
MaeI CTAG 2 cut(s) 36, 401
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 460
MalI GATC 3 cut(s) 62, 161, 274
MboI GATC 3 cut(s) 60, 159, 272
MboII GAAGA 6 cut(s) 169, 203, 322, 343, 584, 649
MflI RGATCY 2 cut(s) 159, 272
MhlI GDGCHC 2 cut(s) 125, 536
MluCI AATT 1 cut(s) 377
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 282
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 2 cut(s) 393, 555
MspA1I CMGCKG 1 cut(s) 127
MspI CCGG 2 cut(s) 146, 164
MspR9I CCNGG 6 cut(s) 146, 164, 278, 283, 484, 602
MvaI CCWGG 4 cut(s) 278, 283, 484, 602
MwoI GCNNNNNNNGC 2 cut(s) 250, 516
NciI CCSGG 2 cut(s) 146, 164
NdeII GATC 3 cut(s) 60, 159, 272
NlaIII CATG 2 cut(s) 339, 547
NmeAIII GCCGAG 2 cut(s) 97, 652
NmuCI GTSAC 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 216
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 465
PdmI GAANNNNTTC 1 cut(s) 377
PflFI GACNNNGTC 2 cut(s) 227, 608
PflMI CCANNNNNTGG 1 cut(s) 288
PkrI GCNGC 1 cut(s) 367
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
PpuMI RGGWCCY 1 cut(s) 106
Psp5II RGGWCCY 1 cut(s) 106
Psp6I CCWGG 4 cut(s) 276, 281, 482, 600
PspGI CCWGG 4 cut(s) 276, 281, 482, 600
PspPI GGNCC 2 cut(s) 106, 291
PspPPI RGGWCCY 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 288
PsuI RGATCY 2 cut(s) 159, 272
PsyI GACNNNGTC 2 cut(s) 227, 608
RsaI GTAC 3 cut(s) 89, 95, 218
RsaNI GTAC 3 cut(s) 88, 94, 217
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 2 cut(s) 393, 555
SatI GCNGC 1 cut(s) 366
Sau3AI GATC 3 cut(s) 60, 159, 272
Sau96I GGNCC 2 cut(s) 106, 291
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 6 cut(s) 146, 164, 278, 283, 484, 602
SduI GDGCHC 2 cut(s) 125, 536
SinI GGWCC 1 cut(s) 106
Sse9I AATT 1 cut(s) 377
SsiI CCGC 3 cut(s) 5, 125, 435
SspMI CTAG 2 cut(s) 36, 401
StyD4I CCNGG 6 cut(s) 144, 162, 276, 281, 482, 600
TaiI ACGT 1 cut(s) 462
TaqI TCGA 2 cut(s) 140, 384
TasI AATT 1 cut(s) 377
TatI WGTACW 2 cut(s) 93, 216
Tru1I TTAA 2 cut(s) 393, 555
Tru9I TTAA 2 cut(s) 393, 555
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 1 cut(s) 460
TspDTI ATGAA 2 cut(s) 370, 532
Tth111I GACNNNGTC 2 cut(s) 227, 608
Van91I CCANNNNNTGG 1 cut(s) 288
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 377
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 2 cut(s) 36, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.