MD10G1196900.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000304
Physical Location & Seq
Reverse (-)
29492441 .. 29499355
6915 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1196900.v1.1.491

Sequence Viewer

Length: 666 bp
ATGGCGGATGAGGTTGTTCTTCTAGACTACTGGGCCAGCAAGTTTGGCATGAGGGCCAGAGTAGCGCTGGCGGAGAAGGGCGTCAAGTATGAGTACAGAGAGGAGGACTTGAGGAATAATATTAAGAGCCAGATGCTTCTGCAGATGAACCCGGTTCACAAGAAGACCCCGGTTCTCATTCATAACGGTAAGCCGGTCTGCGAGTCACTCATTATTGTGCAGTATATTGATGAGGTTTGGAGGGATAAAGCTCCTTTGCTTCCCTCTGAGCCTTACCAGAAAGCTCACTCAAGGTTCTGGGCTGATTTCATTGACAAGAAGCTATATGATGCTTGCACGAAGATATGGATGACAAAGGGAGAGGAACAAGAGGCAGCAAAAAAGGACTTCATCGAAATCCTGAAGCAGTTGGAGGGACAGCTTGGAGACAAGCCATATTTTGAGGGTGAGAAATTTGGGTTCTTGGACATTGCTCTCATCACGTTCTACTGCTGGTTCCATGCATATGAGATCTGTGGAAACTTCAGCATCGAGGCCGAGTGTCCCAAGCTCATTGCATGGGCCAAGAGGTGCATGCAGAGGGAGAGTGTTTCCAAATCTCTTGCTGACGAAAAGAAGGTGTATGAGCTTGTTCTTGGCCTGCAGAAGATGTTTGGTTGGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016740 GO:0016765 GO:0018973 GO:0018974 GO:0019326 GO:0019439 GO:0019748 GO:0019899 GO:0031090 GO:0031668 GO:0033218 GO:0033554 GO:0034641 GO:0042221 GO:0042277 GO:0042537 GO:0042631 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046256 GO:0046260 GO:0046263 GO:0046686 GO:0048037 GO:0048046 GO:0048583 GO:0050789 GO:0050896 GO:0051186 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071944 GO:0072341 GO:0072490 GO:0072491 GO:0080167 GO:0098588 GO:0098754 GO:0098805 GO:0104004 GO:1900750 GO:1901360 GO:1901361 GO:1901564 GO:1901575 GO:1901681 GO:1901700 GO:1901701 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

222

Amino Acids

25.88

Weight (kDa)

6.16

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 6 - 78 4.2e-19 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 78 2.8e-13 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 78 3.5e-11 Glutathione S-transferase, N-terminal domain
GST_C PF00043 118 - 195 3.5e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 123 - 190 7.3e-09 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 71
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 2 cut(s) 422, 508
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 95
AfeI AGCGCT 1 cut(s) 66
AluBI AGCT 6 cut(s) 251, 284, 322, 421, 550, 628
AluI AGCT 6 cut(s) 251, 284, 322, 421, 550, 628
Alw26I GTCTC 1 cut(s) 420
Aor51HI AGCGCT 1 cut(s) 66
AoxI GGCC 5 cut(s) 33, 54, 534, 561, 637
ApeKI GCWGC 1 cut(s) 374
ApoI RAATTY 1 cut(s) 452
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 3 cut(s) 33, 54, 561
AsuC2I CCSGG 2 cut(s) 152, 170
AsuHPI GGTGA 1 cut(s) 458
BbsI GAAGAC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 386
BcnI CCSGG 2 cut(s) 152, 170
BcoDI GTCTC 1 cut(s) 420
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 2 cut(s) 140, 641
BfoI RGCGCY 1 cut(s) 68
BglII AGATCT 1 cut(s) 510
BisI GCNGC 1 cut(s) 375
BlsI GCNGC 1 cut(s) 376
Bme1390I CCNGG 2 cut(s) 152, 170
BmgT120I GGNCC 3 cut(s) 33, 54, 561
BmiI GGNNCC 1 cut(s) 497
BmrFI CCNGG 2 cut(s) 152, 170
BmrI ACTGGG 1 cut(s) 40
BmsI GCATC 3 cut(s) 123, 319, 537
BmuI ACTGGG 1 cut(s) 40
BpiI GAAGAC 1 cut(s) 170
BpuEI CTTGAG 2 cut(s) 130, 274
BpuMI CCSGG 2 cut(s) 152, 170
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 168
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 35
Bse3DI GCAATG 2 cut(s) 468, 552
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 2 cut(s) 13, 354
BseMI GCAATG 2 cut(s) 468, 552
BseMII CTCAG 1 cut(s) 258
BseNI ACTGG 1 cut(s) 35
BseRI GAGGAG 1 cut(s) 116
BseXI GCAGC 1 cut(s) 386
BsgI GTGCAG 1 cut(s) 239
BshFI GGCC 5 cut(s) 35, 56, 536, 563, 639
BsiSI CCGG 3 cut(s) 152, 170, 194
BslFI GGGAC 2 cut(s) 429, 528
BsmAI GTCTC 1 cut(s) 420
BsmFI GGGAC 2 cut(s) 429, 528
BsnI GGCC 5 cut(s) 35, 56, 536, 563, 639
Bsp143I GATC 1 cut(s) 510
BspACI CCGC 2 cut(s) 5, 71
BspANI GGCC 5 cut(s) 35, 56, 536, 563, 639
BspCNI CTCAG 1 cut(s) 259
BspLI GGNNCC 1 cut(s) 497
BspMAI CTGCAG 2 cut(s) 144, 645
BsrDI GCAATG 2 cut(s) 468, 552
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 1 cut(s) 510
BssNI GRCGYC 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 5 cut(s) 37, 69, 334, 575, 641
BstDEI CTNAG 1 cut(s) 267
BstF5I GGATG 2 cut(s) 13, 354
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 1 cut(s) 513
BstMAI GTCTC 1 cut(s) 420
BstMBI GATC 1 cut(s) 510
BstMWI GCNNNNNNNGC 2 cut(s) 45, 62
BstNSI RCATGY 1 cut(s) 577
BstSCI CCNGG 2 cut(s) 150, 168
BstSFI CTRYAG 2 cut(s) 140, 641
BstV1I GCAGC 1 cut(s) 386
BstV2I GAAGAC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 510
BstYI RGATCY 1 cut(s) 510
BsuRI GGCC 5 cut(s) 35, 56, 536, 563, 639
BtsCI GGATG 2 cut(s) 13, 354
Cac8I GCNNGC 5 cut(s) 37, 69, 334, 575, 641
CfoI GCGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 3 cut(s) 33, 54, 561
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 94
CviAII CATG 4 cut(s) 49, 500, 558, 574
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 1 cut(s) 267
DpnI GATC 1 cut(s) 512
DpnII GATC 1 cut(s) 510
EciI GGCGGA 2 cut(s) 20, 86
Eco47III AGCGCT 1 cut(s) 66
Eco57I CTGAAG 2 cut(s) 422, 508
EcoT22I ATGCAT 1 cut(s) 505
FaeI CATG 4 cut(s) 52, 503, 561, 577
FaqI GGGAC 2 cut(s) 429, 528
FatI CATG 4 cut(s) 48, 499, 557, 573
FauNDI CATATG 1 cut(s) 505
Fnu4HI GCNGC 1 cut(s) 375
FokI GGATG 2 cut(s) 20, 361
Fsp4HI GCNGC 1 cut(s) 375
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 66
GluI GCNGC 1 cut(s) 375
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 5 cut(s) 35, 56, 536, 563, 639
HapII CCGG 3 cut(s) 152, 170, 194
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 67
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 4 cut(s) 52, 503, 561, 577
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HinfI GANTC 1 cut(s) 203
HpaII CCGG 3 cut(s) 152, 170, 194
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 1 cut(s) 268
Hpy188III TCNNGA 2 cut(s) 23, 400
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 2 cut(s) 70, 610
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 8 cut(s) 142, 220, 336, 503, 557, 573, 577, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 62
HpyF3I CTNAG 1 cut(s) 267
HpySE526I ACGT 1 cut(s) 482
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 4 cut(s) 52, 503, 561, 577
HspAI GCGC 1 cut(s) 65
Kzo9I GATC 1 cut(s) 510
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 1 cut(s) 386
LweI GCATC 3 cut(s) 123, 319, 537
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 1 cut(s) 204
MalI GATC 1 cut(s) 512
MboI GATC 1 cut(s) 510
MboII GAAGA 4 cut(s) 11, 175, 352, 658
MflI RGATCY 1 cut(s) 510
MluCI AATT 1 cut(s) 452
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 390
Mph1103I ATGCAT 1 cut(s) 505
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 2 cut(s) 215, 504
MspI CCGG 3 cut(s) 152, 170, 194
MspR9I CCNGG 2 cut(s) 152, 170
MwoI GCNNNNNNNGC 2 cut(s) 45, 62
NciI CCSGG 2 cut(s) 152, 170
NdeI CATATG 1 cut(s) 505
NdeII GATC 1 cut(s) 510
NlaIII CATG 4 cut(s) 52, 503, 561, 577
NlaIV GGNNCC 1 cut(s) 497
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 1 cut(s) 204
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 1 cut(s) 577
PaeI GCATGC 1 cut(s) 577
PkrI GCNGC 1 cut(s) 376
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 497
PspPI GGNCC 3 cut(s) 33, 54, 561
PstI CTGCAG 2 cut(s) 144, 645
PsuI RGATCY 1 cut(s) 510
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 2 cut(s) 215, 504
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 1 cut(s) 375
Sau3AI GATC 1 cut(s) 510
Sau96I GGNCC 3 cut(s) 33, 54, 561
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 152, 170
SfaNI GCATC 3 cut(s) 123, 319, 537
SfcI CTRYAG 2 cut(s) 140, 641
SmiMI CAYNNNNRTG 2 cut(s) 215, 504
SmlI CTYRAG 2 cut(s) 109, 289
SmoI CTYRAG 2 cut(s) 109, 289
SphI GCATGC 1 cut(s) 577
Sse9I AATT 1 cut(s) 452
SsiI CCGC 2 cut(s) 5, 71
SspI AATATT 1 cut(s) 121
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 150, 168
TaaI ACNGT 1 cut(s) 188
TaiI ACGT 1 cut(s) 485
TaqI TCGA 2 cut(s) 393, 531
TasI AATT 1 cut(s) 452
TatI WGTACW 1 cut(s) 93
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseFI GTSAC 1 cut(s) 204
TseI GCWGC 1 cut(s) 374
Tsp45I GTSAC 1 cut(s) 204
TspDTI ATGAA 4 cut(s) 161, 170, 298, 379
XapI RAATTY 1 cut(s) 452
XbaI TCTAGA 1 cut(s) 22
XceI RCATGY 1 cut(s) 577
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XspI CTAG 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.