Rroxscaffold_4G00329120

glutathione s-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000304
Physical Location & Seq
Reverse (-)
62321379 .. 62322811
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329120.1

Sequence Viewer

Length: 543 bp
ATGGCGGACGAGGTTATTCTATTGGACGGTTATGTGAGCAGCTTTGGGATGAGGGTCAGAGTAGCTCTGGCCGAGAAGGGAATTAAGTATGAATACAGAGAAGAAGACCTGAGAAACAAGAGCCAACTGCTTCTTAAGATGAACCCGATCCACAAGAAGATCCCGGTGCTCATCCATAATGGCAAACCGGTCTGTGAGTCGCTCATCATTGTGCAGTATATTGATGAGGTATGGAAAGATAAGGCTCCTCTGCTTCCCTCTGATCCTTACCAGAGAGCCCAGGCCAGATTCTGGGCCGATTTCATTGATAAGAAGTTATATGATGCGAGCAGGAAGATATGGACTACAAAAGGAGAAGAGCAGGATGCAGCAAAGAAGGAATTCATTGAAGTCCTGAAGGTGTTGGAAGGAGAGCTTGGAGACAAGCCTTATTTCATGGGTGAGAGATTTGGGTTCCTGGACATTGCTCTCATCACATTCTACAGCTGGTTTCATGCTTTTGAGACACTTGGAAACTTCAGTATAGAGGGCCGAGTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009266 GO:0009404 GO:0009407 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016740 GO:0016765 GO:0018973 GO:0018974 GO:0019326 GO:0019439 GO:0019748 GO:0019899 GO:0031090 GO:0031668 GO:0033218 GO:0033554 GO:0034641 GO:0042221 GO:0042277 GO:0042537 GO:0042631 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043603 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046256 GO:0046260 GO:0046263 GO:0046686 GO:0048037 GO:0048046 GO:0048583 GO:0050789 GO:0050896 GO:0051186 GO:0051716 GO:0065007 GO:0070887 GO:0071214 GO:0071229 GO:0071462 GO:0071496 GO:0071704 GO:0071944 GO:0072341 GO:0072490 GO:0072491 GO:0080167 GO:0098588 GO:0098754 GO:0098805 GO:0104004 GO:1900750 GO:1901360 GO:1901361 GO:1901564 GO:1901575 GO:1901681 GO:1901700 GO:1901701 GO:2000030
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_C_2 GST_N GST_N_3

Protein Analysis

180

Amino Acids

20.92

Weight (kDa)

5.98

Isoelectric Point (pI)

29.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 5 - 76 1.6e-20 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 11 - 76 3.6e-16 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 13 - 76 6.8e-14 Glutathione S-transferase, N-terminal domain
GST_C PF00043 116 - 167 2.7e-07 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 121 - 166 1.9e-08 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17170 AT1G17180 AT1G78340 AT1G78370 AT1G78380
fragaria_vesca FvH4_3g16210 FvH4_3g16230 FvH4_3g16240 FvH4_3g16240 FvH4_3g16250 FvH4_3g16290 FvH4_4g11790
malus_domestica MD04G1033800.v1.1 MD05G1209700.v1.1 MD05G1210000.v1.1 MD05G1210100.v1.1 MD05G1210200.v1.1 MD05G1210400.v1.1 MD05G1210600.v1.1 MD05G1210700.v1.1 MD05G1211000.v1.1 MD05G1211400.v1.1 MD10G1196300.v1.1 MD10G1196600.v1.1 MD10G1196900.v1.1 MD10G1197100.v1.1
prunus_persica Prupe.1G180800_v2.0.a1 Prupe.4G145800_v2.0.a1 Prupe.4G145900_v2.0.a1 Prupe.4G146000_v2.0.a1 Prupe.4G146100_v2.0.a1 Prupe.4G146200_v2.0.a1 Prupe.4G146400_v2.0.a1 Prupe.4G146500_v2.0.a1 Prupe.4G146800_v2.0.a1 Prupe.4G147000_v2.0.a1 Prupe.4G147200_v2.0.a1 Prupe.4G147400_v2.0.a1 Prupe.4G147800_v2.0.a1
pyrus_communis pycom05g19570 pycom05g19590 pycom05g19600 pycom05g19620 pycom05g19630 pycom10g16980
rosa_chinensis RchiOBHm_Chr5g0026581 RchiOBHm_Chr5g0027291 RchiOBHm_Chr5g0027311 RchiOBHm_Chr5g0027331 RchiOBHm_Chr5g0027341 RchiOBHm_Chr5g0027351 RchiOBHm_Chr5g0027361 RchiOBHm_Chr5g0027381 RchiOBHm_Chr5g0027391
rosa_laevigata RLG00000023802 RLG00000032937 RLG00000033008 RLG00000033009 RLG00000033010 RLG00000033011 RLG00000033012 RLG00000033014 RLG00000033015
rosa_multiflora Rmu_sc0001348.1_g000012 Rmu_sc0001348.1_g000018 Rmu_sc0001526.1_g000022 Rmu_sc0003500.1_g000007 Rmu_sc0003500.1_g000014 Rmu_sc0003500.1_g000018 Rmu_sc0003500.1_g000022 Rmu_sc0003500.1_g000023 Rmu_sc0003500.1_g000027 Rmu_sc0003500.1_g000035 Rmu_sc0004371.1_g000001 Rmu_sc0006854.1_g000010 Rmu_sc0006854.1_g000013 Rmu_sc0007802.1_g000003 Rmu_sc0007802.1_g000006 Rmu_sc0007802.1_g000008 Rmu_sc0007802.1_g000011 Rmu_sc0007802.1_g000012 Rmu_sc0041677.1_g000002 Rmu_ssc0000372.1_g000062
rosa_roxburghii Rroxscaffold_1G00052130 Rroxscaffold_1G00052140 Rroxscaffold_1G00052160 Rroxscaffold_1G00052170 Rroxscaffold_1G00052180 Rroxscaffold_1G00052200 Rroxscaffold_1G00052900 Rroxscaffold_4G00329120 Rroxscaffold_4G00329130 Rroxscaffold_6G00405750
rosa_rugosa Rorug01G0093600 Rorug03G0151900 Rorug05G0098200 Rorug05G0098300 Rorug05G0098400 Rorug05G0098500
rosa_samantha Rh3AG202100 Rh5AG187300 Rh5AG192300 Rh5AG192500 Rh5AG192600 Rh5AG192700 Rh5BG190100 Rh5BG190200 Rh5CG203800 Rh5CG210500 Rh5CG210600 Rh5CG210700 Rh5CG210800
rosa_wichuraiana Rw5G016960 Rw5G017500 Rw5G017510 Rw5G017520 Rw5G017530 Rw5G017540 Rw5G017550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 291
AciI CCGC 1 cut(s) 5
AclWI GGATC 3 cut(s) 142, 154, 257
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 1 cut(s) 380
AcuI CTGAAG 2 cut(s) 416, 502
AfiI CCNNNNNNNGG 1 cut(s) 291
AflII CTTAAG 1 cut(s) 134
AgeI ACCGGT 1 cut(s) 187
AgsI TTSAA 1 cut(s) 389
AjnI CCWGG 2 cut(s) 279, 456
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 4 cut(s) 42, 65, 415, 486
AluI AGCT 4 cut(s) 42, 65, 415, 486
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 2 cut(s) 414, 497
AlwI GGATC 3 cut(s) 142, 154, 257
AlwNI CAGNNNCTG 1 cut(s) 291
AoxI GGCC 4 cut(s) 69, 282, 294, 529
ApeKI GCWGC 2 cut(s) 39, 368
ApoI RAATTY 1 cut(s) 380
AsiGI ACCGGT 1 cut(s) 187
Asp700I GAANNNNTTC 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 294, 529
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 452
BaeGI GKGCMC 1 cut(s) 540
BanII GRGCYC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 51, 380
BciT130I CCWGG 2 cut(s) 281, 458
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 2 cut(s) 414, 497
BfmI CTRYAG 1 cut(s) 481
BfrI CTTAAG 1 cut(s) 134
BisI GCNGC 2 cut(s) 40, 369
BlsI GCNGC 2 cut(s) 41, 370
Bme1390I CCNGG 3 cut(s) 164, 281, 458
BmgT120I GGNCC 2 cut(s) 294, 529
BmiI GGNNCC 2 cut(s) 246, 455
BmrFI CCNGG 3 cut(s) 164, 281, 458
BmsI GCATC 2 cut(s) 313, 355
BpiI GAAGAC 1 cut(s) 111
BpuMI CCSGG 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 279
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 291
Bse118I RCCGGY 1 cut(s) 187
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 2 cut(s) 281, 458
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 3 cut(s) 54, 171, 370
BseLI CCNNNNNNNGG 1 cut(s) 291
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 237
BseSI GKGCMC 1 cut(s) 540
BseXI GCAGC 2 cut(s) 51, 380
BsgI GTGCAG 1 cut(s) 233
BshFI GGCC 4 cut(s) 71, 284, 296, 531
BshTI ACCGGT 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 2 cut(s) 164, 188
BslI CCNNNNNNNGG 1 cut(s) 291
BsmAI GTCTC 2 cut(s) 414, 497
BsnI GGCC 4 cut(s) 71, 284, 296, 531
Bsp1286I GDGCHC 3 cut(s) 171, 280, 540
Bsp143I GATC 3 cut(s) 147, 159, 262
BspACI CCGC 1 cut(s) 5
BspANI GGCC 4 cut(s) 71, 284, 296, 531
BspCNI CTCAG 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 246, 455
BspPI GGATC 3 cut(s) 142, 154, 257
BspQI GCTCTTC 1 cut(s) 351
BspTI CTTAAG 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 187
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 3 cut(s) 147, 159, 262
Bst2UI CCWGG 2 cut(s) 281, 458
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 351
BstAFI CTTAAG 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 1 cut(s) 110
BstF5I GGATG 3 cut(s) 54, 171, 370
BstKTI GATC 3 cut(s) 150, 162, 265
BstMAI GTCTC 2 cut(s) 414, 497
BstMBI GATC 3 cut(s) 147, 159, 262
BstNI CCWGG 2 cut(s) 281, 458
BstSCI CCNGG 3 cut(s) 162, 279, 456
BstSFI CTRYAG 1 cut(s) 481
BstSLI GKGCMC 1 cut(s) 540
BstV1I GCAGC 2 cut(s) 51, 380
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 4 cut(s) 71, 284, 296, 531
BtsCI GGATG 3 cut(s) 54, 171, 370
Cac8I GCNNGC 1 cut(s) 328
CaiI CAGNNNCTG 1 cut(s) 291
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 294, 529
CspAI ACCGGT 1 cut(s) 187
CviAII CATG 2 cut(s) 436, 494
DdeI CTNAG 1 cut(s) 110
DpnI GATC 3 cut(s) 149, 161, 264
DpnII GATC 3 cut(s) 147, 159, 262
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 280
Eco57I CTGAAG 2 cut(s) 416, 502
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 2 cut(s) 279, 456
EcoT38I GRGCYC 1 cut(s) 280
FaeI CATG 2 cut(s) 439, 497
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FatI CATG 2 cut(s) 435, 493
Fnu4HI GCNGC 2 cut(s) 40, 369
FokI GGATG 3 cut(s) 61, 158, 377
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 2 cut(s) 40, 369
GluI GCNGC 2 cut(s) 40, 369
HaeIII GGCC 4 cut(s) 71, 284, 296, 531
HapII CCGG 2 cut(s) 164, 188
Hin1II CATG 2 cut(s) 439, 497
HinfI GANTC 2 cut(s) 197, 288
HpaII CCGG 2 cut(s) 164, 188
HphI GGTGA 1 cut(s) 452
Hpy188I TCNGA 2 cut(s) 59, 262
Hpy188III TCNNGA 1 cut(s) 394
HpyAV CCTTC 4 cut(s) 70, 370, 391, 401
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4V TGCA 2 cut(s) 214, 368
HpyF3I CTNAG 1 cut(s) 110
Hsp92II CATG 2 cut(s) 439, 497
Kzo9I GATC 3 cut(s) 147, 159, 262
LguI GCTCTTC 1 cut(s) 351
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 2 cut(s) 51, 380
LweI GCATC 2 cut(s) 313, 355
MalI GATC 3 cut(s) 149, 161, 264
MboI GATC 3 cut(s) 147, 159, 262
MboII GAAGA 5 cut(s) 113, 116, 169, 346, 368
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 3 cut(s) 171, 280, 540
MluCI AATT 2 cut(s) 81, 380
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 6 cut(s) 4, 45, 220, 258, 268, 520
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 2 cut(s) 84, 135
MslI CAYNNNNRTG 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 486
MspCI CTTAAG 1 cut(s) 134
MspI CCGG 2 cut(s) 164, 188
MspR9I CCNGG 3 cut(s) 164, 281, 458
MvaI CCWGG 2 cut(s) 281, 458
NciI CCSGG 1 cut(s) 164
NdeII GATC 3 cut(s) 147, 159, 262
NlaIII CATG 2 cut(s) 439, 497
NlaIV GGNNCC 2 cut(s) 246, 455
NmeAIII GCCGAG 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 288
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 187
PkrI GCNGC 2 cut(s) 41, 370
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 279, 456
PspGI CCWGG 2 cut(s) 279, 456
PspN4I GGNNCC 2 cut(s) 246, 455
PspPI GGNCC 2 cut(s) 294, 529
PstNI CAGNNNCTG 1 cut(s) 291
PsuI RGATCY 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 486
RseI CAYNNNNRTG 1 cut(s) 209
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 2 cut(s) 84, 135
SatI GCNGC 2 cut(s) 40, 369
Sau3AI GATC 3 cut(s) 147, 159, 262
Sau96I GGNCC 2 cut(s) 294, 529
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 3 cut(s) 164, 281, 458
SduI GDGCHC 3 cut(s) 171, 280, 540
SetI ASST 8 cut(s) 15, 44, 67, 111, 231, 402, 417, 488
SfaNI GCATC 2 cut(s) 313, 355
SfcI CTRYAG 1 cut(s) 481
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 2 cut(s) 81, 380
SsiI CCGC 1 cut(s) 5
StyD4I CCNGG 3 cut(s) 162, 279, 456
TaaI ACNGT 1 cut(s) 29
TasI AATT 2 cut(s) 81, 380
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 2 cut(s) 84, 135
Tru9I TTAA 2 cut(s) 84, 135
TseI GCWGC 2 cut(s) 39, 368
TspDTI ATGAA 6 cut(s) 105, 155, 292, 373, 424, 482
Van91I CCANNNNNTGG 1 cut(s) 291
Vha464I CTTAAG 1 cut(s) 134
XapI RAATTY 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.