belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 306, 794 |
| Acc65I | GGTACC | 1 cut(s) | 356 |
| AccB1I | GGYRCC | 1 cut(s) | 356 |
| AciI | CCGC | 2 cut(s) | 392, 566 |
| AclWI | GGATC | 3 cut(s) | 43, 140, 155 |
| AcoI | YGGCCR | 1 cut(s) | 696 |
| AcsI | RAATTY | 2 cut(s) | 513, 664 |
| AfaI | GTAC | 4 cut(s) | 358, 403, 488, 815 |
| AflIII | ACRYGT | 1 cut(s) | 489 |
| AgsI | TTSAA | 3 cut(s) | 427, 548, 732 |
| AjnI | CCWGG | 1 cut(s) | 754 |
| AleI | CACNNNNGTG | 1 cut(s) | 804 |
| AluBI | AGCT | 7 cut(s) | 138, 153, 168, 553, 703, 819, 885 |
| AluI | AGCT | 7 cut(s) | 138, 153, 168, 553, 703, 819, 885 |
| Alw21I | GWGCWC | 1 cut(s) | 475 |
| Alw26I | GTCTC | 2 cut(s) | 454, 708 |
| AlwI | GGATC | 3 cut(s) | 43, 140, 155 |
| AoxI | GGCC | 1 cut(s) | 696 |
| ApeKI | GCWGC | 3 cut(s) | 135, 764, 829 |
| ApoI | RAATTY | 2 cut(s) | 513, 664 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 552, 648, 668 |
| Asp718I | GGTACC | 1 cut(s) | 356 |
| BalI | TGGCCA | 1 cut(s) | 698 |
| BanI | GGYRCC | 1 cut(s) | 356 |
| BbsI | GAAGAC | 1 cut(s) | 431 |
| Bbv12I | GWGCWC | 1 cut(s) | 475 |
| BbvI | GCAGC | 3 cut(s) | 147, 751, 816 |
| BccI | CCATC | 1 cut(s) | 230 |
| BceAI | ACGGC | 1 cut(s) | 591 |
| BciT130I | CCWGG | 1 cut(s) | 756 |
| BciVI | GTATCC | 1 cut(s) | 10 |
| BcoDI | GTCTC | 2 cut(s) | 454, 708 |
| BfaI | CTAG | 1 cut(s) | 579 |
| BfuI | GTATCC | 1 cut(s) | 10 |
| BisI | GCNGC | 3 cut(s) | 136, 765, 830 |
| BlsI | GCNGC | 3 cut(s) | 137, 766, 831 |
| Bme1390I | CCNGG | 1 cut(s) | 756 |
| BmiI | GGNNCC | 1 cut(s) | 358 |
| BmrFI | CCNGG | 1 cut(s) | 756 |
| BmsI | GCATC | 1 cut(s) | 31 |
| BpiI | GAAGAC | 1 cut(s) | 431 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 736, 768 |
| BsaBI | GATNNNNATC | 1 cut(s) | 92 |
| BsaJI | CCNNGG | 1 cut(s) | 360 |
| Bse3DI | GCAATG | 1 cut(s) | 561 |
| Bse8I | GATNNNNATC | 1 cut(s) | 92 |
| BseBI | CCWGG | 1 cut(s) | 756 |
| BseDI | CCNNGG | 1 cut(s) | 360 |
| BseGI | GGATG | 1 cut(s) | 664 |
| BseJI | GATNNNNATC | 1 cut(s) | 92 |
| BseMI | GCAATG | 1 cut(s) | 561 |
| BseMII | CTCAG | 1 cut(s) | 341 |
| BseXI | GCAGC | 3 cut(s) | 147, 751, 816 |
| BseYI | CCCAGC | 1 cut(s) | 781 |
| BsgI | GTGCAG | 1 cut(s) | 750 |
| BshFI | GGCC | 1 cut(s) | 698 |
| BshNI | GGYRCC | 1 cut(s) | 356 |
| BsiHKAI | GWGCWC | 1 cut(s) | 475 |
| BslFI | GGGAC | 1 cut(s) | 586 |
| BsmAI | GTCTC | 2 cut(s) | 454, 708 |
| BsmFI | GGGAC | 1 cut(s) | 586 |
| BsmI | GAATGC | 2 cut(s) | 611, 831 |
| BsnI | GGCC | 1 cut(s) | 698 |
| Bsp1286I | GDGCHC | 1 cut(s) | 475 |
| Bsp1407I | TGTACA | 1 cut(s) | 813 |
| Bsp143I | GATC | 4 cut(s) | 35, 87, 145, 160 |
| BspACI | CCGC | 2 cut(s) | 392, 566 |
| BspANI | GGCC | 1 cut(s) | 698 |
| BspCNI | CTCAG | 1 cut(s) | 342 |
| BspHI | TCATGA | 3 cut(s) | 157, 556, 862 |
| BspLI | GGNNCC | 1 cut(s) | 358 |
| BspPI | GGATC | 3 cut(s) | 43, 140, 155 |
| BspT107I | GGYRCC | 1 cut(s) | 356 |
| BsrDI | GCAATG | 1 cut(s) | 561 |
| BsrGI | TGTACA | 1 cut(s) | 813 |
| BssECI | CCNNGG | 1 cut(s) | 360 |
| BssMI | GATC | 4 cut(s) | 35, 87, 145, 160 |
| BssT1I | CCWWGG | 1 cut(s) | 360 |
| Bst2UI | CCWGG | 1 cut(s) | 756 |
| Bst4CI | ACNGT | 1 cut(s) | 465 |
| BstAUI | TGTACA | 1 cut(s) | 813 |
| BstC8I | GCNNGC | 1 cut(s) | 611 |
| BstDEI | CTNAG | 1 cut(s) | 350 |
| BstF5I | GGATG | 1 cut(s) | 664 |
| BstKTI | GATC | 4 cut(s) | 38, 90, 148, 163 |
| BstMAI | GTCTC | 2 cut(s) | 454, 708 |
| BstMBI | GATC | 4 cut(s) | 35, 87, 145, 160 |
| BstNI | CCWGG | 1 cut(s) | 756 |
| BstNSI | RCATGY | 1 cut(s) | 493 |
| BstSCI | CCNGG | 1 cut(s) | 754 |
| BstV1I | GCAGC | 3 cut(s) | 147, 751, 816 |
| BstV2I | GAAGAC | 1 cut(s) | 431 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 277, 777 |
| BsuI | GTATCC | 1 cut(s) | 10 |
| BsuRI | GGCC | 1 cut(s) | 698 |
| BtsCI | GGATG | 1 cut(s) | 664 |
| BtsI | GCAGTG | 1 cut(s) | 345 |
| BtsIMutI | CAGTG | 1 cut(s) | 345 |
| Cac8I | GCNNGC | 1 cut(s) | 611 |
| CciI | TCATGA | 3 cut(s) | 157, 556, 862 |
| Csp6I | GTAC | 4 cut(s) | 357, 402, 487, 814 |
| CviAII | CATG | 6 cut(s) | 158, 388, 443, 490, 557, 863 |
| CviQI | GTAC | 4 cut(s) | 357, 402, 487, 814 |
| DdeI | CTNAG | 1 cut(s) | 350 |
| DpnI | GATC | 4 cut(s) | 37, 89, 147, 162 |
| DpnII | GATC | 4 cut(s) | 35, 87, 145, 160 |
| EaeI | YGGCCR | 1 cut(s) | 696 |
| EciI | GGCGGA | 1 cut(s) | 407 |
| Eco130I | CCWWGG | 1 cut(s) | 360 |
| EcoRII | CCWGG | 1 cut(s) | 754 |
| EcoT14I | CCWWGG | 1 cut(s) | 360 |
| ErhI | CCWWGG | 1 cut(s) | 360 |
| FaeI | CATG | 6 cut(s) | 161, 391, 446, 493, 560, 866 |
| FaqI | GGGAC | 1 cut(s) | 586 |
| FatI | CATG | 6 cut(s) | 157, 387, 442, 489, 556, 862 |
| Fnu4HI | GCNGC | 3 cut(s) | 136, 765, 830 |
| FokI | GGATG | 1 cut(s) | 671 |
| Fsp4HI | GCNGC | 3 cut(s) | 136, 765, 830 |
| FspBI | CTAG | 1 cut(s) | 579 |
| GluI | GCNGC | 3 cut(s) | 136, 765, 830 |
| GsaI | CCCAGC | 1 cut(s) | 785 |
| HaeIII | GGCC | 1 cut(s) | 698 |
| Hin1II | CATG | 6 cut(s) | 161, 391, 446, 493, 560, 866 |
| HindIII | AAGCTT | 3 cut(s) | 151, 166, 551 |
| HinfI | GANTC | 2 cut(s) | 415, 859 |
| Hpy166II | GTNNAC | 3 cut(s) | 231, 404, 814 |
| Hpy188I | TCNGA | 6 cut(s) | 16, 40, 126, 142, 249, 351 |
| Hpy188III | TCNNGA | 6 cut(s) | 158, 285, 557, 838, 863, 878 |
| Hpy8I | GTNNAC | 3 cut(s) | 231, 404, 814 |
| HpyAV | CCTTC | 2 cut(s) | 337, 849 |
| HpyCH4III | ACNGT | 1 cut(s) | 465 |
| HpyCH4V | TGCA | 6 cut(s) | 44, 111, 254, 598, 708, 767 |
| HpyF3I | CTNAG | 1 cut(s) | 350 |
| Hsp92II | CATG | 6 cut(s) | 161, 391, 446, 493, 560, 866 |
| KpnI | GGTACC | 1 cut(s) | 360 |
| Kzo9I | GATC | 4 cut(s) | 35, 87, 145, 160 |
| Lsp1109I | GCAGC | 3 cut(s) | 147, 751, 816 |
| LweI | GCATC | 1 cut(s) | 31 |
| MaeI | CTAG | 1 cut(s) | 579 |
| MaeIII | GTNAC | 1 cut(s) | 8 |
| MalI | GATC | 4 cut(s) | 37, 89, 147, 162 |
| MboI | GATC | 4 cut(s) | 35, 87, 145, 160 |
| MboII | GAAGA | 6 cut(s) | 97, 431, 614, 661, 864, 866 |
| MhlI | GDGCHC | 1 cut(s) | 475 |
| MlsI | TGGCCA | 1 cut(s) | 698 |
| MluCI | AATT | 5 cut(s) | 190, 372, 383, 513, 664 |
| MluNI | TGGCCA | 1 cut(s) | 698 |
| MnlI | CCTC | 4 cut(s) | 281, 345, 575, 738 |
| Mox20I | TGGCCA | 1 cut(s) | 698 |
| MroXI | GAANNNNTTC | 3 cut(s) | 552, 648, 668 |
| MscI | TGGCCA | 1 cut(s) | 698 |
| MseI | TTAA | 1 cut(s) | 870 |
| MslI | CAYNNNNRTG | 1 cut(s) | 804 |
| Msp20I | TGGCCA | 1 cut(s) | 698 |
| MspR9I | CCNGG | 1 cut(s) | 756 |
| Mva1269I | GAATGC | 2 cut(s) | 611, 831 |
| MvaI | CCWGG | 1 cut(s) | 756 |
| NdeII | GATC | 4 cut(s) | 35, 87, 145, 160 |
| NlaIII | CATG | 6 cut(s) | 161, 391, 446, 493, 560, 866 |
| NlaIV | GGNNCC | 1 cut(s) | 358 |
| NspI | RCATGY | 1 cut(s) | 493 |
| OliI | CACNNNNGTG | 1 cut(s) | 804 |
| PagI | TCATGA | 3 cut(s) | 157, 556, 862 |
| PciI | ACATGT | 1 cut(s) | 489 |
| PctI | GAATGC | 2 cut(s) | 611, 831 |
| PdmI | GAANNNNTTC | 3 cut(s) | 552, 648, 668 |
| PfeI | GAWTC | 2 cut(s) | 415, 859 |
| PkrI | GCNGC | 3 cut(s) | 137, 766, 831 |
| PscI | ACATGT | 1 cut(s) | 489 |
| PsiI | TTATAA | 2 cut(s) | 306, 794 |
| Psp6I | CCWGG | 1 cut(s) | 754 |
| PspFI | CCCAGC | 1 cut(s) | 781 |
| PspGI | CCWGG | 1 cut(s) | 754 |
| PspN4I | GGNNCC | 1 cut(s) | 358 |
| RsaI | GTAC | 4 cut(s) | 358, 403, 488, 815 |
| RsaNI | GTAC | 4 cut(s) | 357, 402, 487, 814 |
| RseI | CAYNNNNRTG | 1 cut(s) | 804 |
| SaqAI | TTAA | 1 cut(s) | 870 |
| SatI | GCNGC | 3 cut(s) | 136, 765, 830 |
| Sau3AI | GATC | 4 cut(s) | 35, 87, 145, 160 |
| ScrFI | CCNGG | 1 cut(s) | 756 |
| SduI | GDGCHC | 1 cut(s) | 475 |
| SfaNI | GCATC | 1 cut(s) | 31 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 804 |
| Sse9I | AATT | 5 cut(s) | 190, 372, 383, 513, 664 |
| SsiI | CCGC | 2 cut(s) | 392, 566 |
| SspI | AATATT | 1 cut(s) | 649 |
| SspMI | CTAG | 1 cut(s) | 579 |
| StyD4I | CCNGG | 1 cut(s) | 754 |
| StyI | CCWWGG | 1 cut(s) | 360 |
| TaaI | ACNGT | 1 cut(s) | 465 |
| TaqI | TCGA | 2 cut(s) | 5, 837 |
| TasI | AATT | 5 cut(s) | 190, 372, 383, 513, 664 |
| TatI | WGTACW | 3 cut(s) | 401, 486, 813 |
| TfiI | GAWTC | 2 cut(s) | 415, 859 |
| Tru1I | TTAA | 1 cut(s) | 870 |
| Tru9I | TTAA | 1 cut(s) | 870 |
| TscAI | CASTG | 1 cut(s) | 345 |
| TseI | GCWGC | 3 cut(s) | 135, 764, 829 |
| TspDTI | ATGAA | 5 cut(s) | 146, 376, 407, 431, 545 |
| TspRI | CASTG | 1 cut(s) | 345 |
| XapI | RAATTY | 2 cut(s) | 513, 664 |
| XceI | RCATGY | 1 cut(s) | 493 |
| XmnI | GAANNNNTTC | 3 cut(s) | 552, 648, 668 |
| XspI | CTAG | 1 cut(s) | 579 |